##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364527_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1189487 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.40933780697057 31.0 30.0 33.0 26.0 34.0 2 29.293559324313758 31.0 30.0 34.0 24.0 34.0 3 29.565954062549654 31.0 30.0 34.0 25.0 34.0 4 33.08130984197389 35.0 33.0 37.0 27.0 37.0 5 32.47625741180862 35.0 33.0 37.0 25.0 37.0 6 32.47222121805451 35.0 33.0 37.0 24.0 37.0 7 32.235914305915074 35.0 32.0 37.0 22.0 37.0 8 32.14645305076895 35.0 32.0 37.0 19.0 37.0 9 33.29075307254304 37.0 33.0 39.0 18.0 39.0 10-11 33.204893369999 37.0 33.0 39.0 17.0 39.0 12-13 33.08124594888385 37.0 32.0 39.0 17.0 39.0 14-15 33.92866756845598 38.0 33.0 41.0 17.0 41.0 16-17 33.95049126219959 38.0 33.0 41.0 17.0 41.0 18-19 33.98815581843265 38.5 33.0 41.0 17.5 41.0 20-21 34.01745122056819 39.0 33.0 41.0 18.0 41.0 22-23 33.950114208898455 39.0 33.0 41.0 18.0 41.0 24-25 33.88984410926727 38.0 33.0 41.0 17.5 41.0 26-27 33.71351221156684 38.0 32.0 41.0 17.0 41.0 28-29 33.518497049568424 38.0 32.0 40.0 16.0 41.0 30-31 33.26699156863421 38.0 32.0 40.0 13.0 41.0 32-33 32.95162620524646 38.0 32.0 40.0 9.5 41.0 34-35 32.70532002451477 38.0 31.0 40.0 8.5 41.0 36-37 32.43626285953525 37.0 31.0 40.0 8.0 41.0 38-39 32.22596547923601 37.0 31.0 40.0 4.0 41.0 40-41 32.19024671980442 37.0 31.0 40.0 2.0 41.0 42-43 31.991168882047468 37.0 30.0 40.0 2.0 41.0 44-45 31.760286997672104 37.0 30.0 40.0 2.0 41.0 46-47 31.413218051143055 37.0 30.0 40.0 2.0 41.0 48-49 31.043450243676475 37.0 29.0 40.0 2.0 41.0 50-51 30.84677806482963 36.5 29.0 40.0 2.0 41.0 52-53 30.659660425040375 36.0 29.0 40.0 2.0 41.0 54-55 30.44935547845416 36.0 28.0 40.0 2.0 41.0 56-57 30.21179634581967 35.0 28.0 40.0 2.0 41.0 58-59 29.992618666702537 35.0 28.0 39.5 2.0 41.0 60-61 29.734198860517182 35.0 28.0 39.0 2.0 41.0 62-63 29.535052505828144 35.0 27.5 39.0 2.0 41.0 64-65 29.387477963189173 35.0 28.0 39.0 2.0 41.0 66-67 29.081224931420017 35.0 27.0 38.5 2.0 40.5 68-69 28.802235333383216 35.0 26.5 37.5 2.0 40.0 70-71 28.49820594928738 34.5 26.0 37.0 2.0 40.0 72-73 28.239111062163772 34.0 26.0 36.5 2.0 39.0 74-75 27.914591332229776 34.0 26.0 36.0 2.0 39.0 76-77 27.623274991656068 34.0 26.0 36.0 2.0 39.0 78-79 27.364232648192036 34.0 26.0 35.0 2.0 37.5 80-81 27.11415383270267 34.0 25.0 35.0 2.0 37.0 82-83 26.844866316319557 34.0 25.0 35.0 2.0 36.5 84-85 26.63907339886859 34.0 24.5 35.0 2.0 36.0 86-87 26.43485132666435 33.5 24.0 35.0 2.0 36.0 88-89 26.256320161548636 33.0 24.0 35.0 2.0 35.5 90-91 26.110388764231978 33.0 23.0 35.0 2.0 35.0 92-93 25.998950387856276 33.0 23.0 35.0 2.0 35.0 94-95 25.875685064233572 33.0 23.0 35.0 2.0 35.0 96-97 25.75001618344715 33.0 20.0 35.0 2.0 35.0 98-99 25.652036129861024 33.0 20.0 35.0 2.0 35.0 100-101 25.524838018406253 33.0 20.0 35.0 2.0 35.0 102-103 24.274976103143622 30.5 17.5 34.0 2.0 35.0 104-105 25.175954844399307 32.5 19.0 35.0 2.0 35.0 106-107 25.32211112857896 33.0 19.0 35.0 2.0 35.0 108-109 25.31421024357559 33.0 18.5 35.0 2.0 35.0 110-111 25.209249869901896 33.0 18.0 35.0 2.0 35.0 112-113 25.100783362911912 33.0 17.0 35.0 2.0 35.0 114-115 24.981708080878562 33.0 12.5 35.0 2.0 35.0 116-117 24.832433645764937 33.0 7.0 35.0 2.0 35.0 118-119 24.675497504386342 33.0 2.0 35.0 2.0 35.0 120-121 24.46157629297336 33.0 2.0 35.0 2.0 35.0 122-123 24.142740105608553 33.0 2.0 35.0 2.0 35.0 124-125 22.474367941810208 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1281896501692152 1101 2 1.4690564004371396 1101 3 1.1435757776531474 1101 4 1.0422735914220738 1101 5 1.267550456502093 1101 6 1.1048183476484112 1101 7 1.316714696844791 1101 8 1.185807685169486 1101 9 1.5344835017527174 1101 10-11 1.5757766608893817 1101 12-13 1.5181860956628412 1101 14-15 1.7695734232783593 1101 16-17 1.6661119260271207 1101 18-19 1.7323235138535011 1101 20-21 1.7303657833465635 1101 22-23 1.6838285599907579 1101 24-25 1.683408792256401 1101 26-27 1.85454778831609 1101 28-29 2.060070157229603 1101 30-31 2.094034433454759 1101 32-33 2.0413630010427255 1101 34-35 2.1002957370537487 1101 36-37 1.8777090960908822 1101 38-39 1.9603506731603986 1101 40-41 1.840017958739054 1101 42-43 1.9164466538632077 1101 44-45 1.8822814049935985 1101 46-47 2.059907703555318 1101 48-49 2.2322793323608394 1101 50-51 2.0965172657090605 1101 52-53 2.0874974295852233 1101 54-55 1.9959765234750293 1101 56-57 1.9475675223282245 1101 58-59 1.9895770418971281 1101 60-61 1.8316503051374013 1101 62-63 1.8757500787972425 1101 64-65 1.6930047266549622 1101 66-67 1.894375218415373 1101 68-69 1.9606355305963916 1101 70-71 1.9628628139914603 1101 72-73 1.858889439628733 1101 74-75 1.753714614051649 1101 76-77 1.9563414519778384 1101 78-79 1.9957479589773826 1101 80-81 1.7402219140448807 1101 82-83 1.8812266063605776 1101 84-85 1.8385299046110433 1101 86-87 1.9108599846374332 1101 88-89 2.0056166046099584 1101 90-91 1.8095845716413024 1101 92-93 1.7221912113507614 1101 94-95 1.8577272489665084 1101 96-97 1.9351445682972894 1101 98-99 1.951515489568859 1101 100-101 1.8938218218358465 1101 102-103 1.7017281222125789 1101 104-105 1.5986363916335904 1101 106-107 1.7484160344366693 1101 108-109 1.7064279301458285 1101 110-111 1.6125822177331486 1101 112-113 1.6355336897632355 1101 114-115 1.7279290277444659 1101 116-117 1.7917270510078893 1101 118-119 1.8666685108686636 1101 120-121 1.7712071251417 1101 122-123 1.7946511420801237 1101 124-125 2.0195101992628643 1102 1 0.9344011769639557 1102 2 -0.015679157365354968 1102 3 0.5186630712156699 1102 4 0.7308675920961534 1102 5 1.063598720742064 1102 6 0.9038573769709615 1102 7 0.8418374470975749 1102 8 0.9346939676096611 1102 9 1.5140092179643787 1102 10-11 1.5469362159955438 1102 12-13 1.6177170697107002 1102 14-15 1.7336868037974043 1102 16-17 1.7571872209378014 1102 18-19 1.9095061225491605 1102 20-21 1.6737092279033519 1102 22-23 1.7637010237588697 1102 24-25 1.9099459004464876 1102 26-27 1.9671989511067949 1102 28-29 1.413237666498219 1102 30-31 1.6426849221638875 1102 32-33 1.9254201631593446 1102 34-35 1.9408104657359146 1102 36-37 1.6012433057302502 1102 38-39 0.9754362141108501 1102 40-41 0.4856246995546911 1102 42-43 0.8202994344496553 1102 44-45 0.6723178053980448 1102 46-47 0.7578238478087691 1102 48-49 0.4654863428091005 1102 50-51 0.3519447008287102 1102 52-53 0.17949095840220863 1102 54-55 0.22925930069106926 1102 56-57 0.31152869523014814 1102 58-59 0.30408179417215564 1102 60-61 0.2761708309210249 1102 62-63 0.1245916022215745 1102 64-65 0.15809714323737012 1102 66-67 -0.029219928197367295 1102 68-69 0.4015787055205564 1102 70-71 -0.18957089008673478 1102 72-73 -0.11782104233958535 1102 74-75 0.23233962247766726 1102 76-77 -0.18002983214753954 1102 78-79 -0.05123485194273769 1102 80-81 -0.15625803202859245 1102 82-83 -0.430874236241376 1102 84-85 -0.2265847566629695 1102 86-87 -0.05323371269994581 1102 88-89 -0.31361224608097515 1102 90-91 -0.23821549576685186 1102 92-93 0.008087672422547598 1102 94-95 0.14995967228972873 1102 96-97 -0.052700123310398084 1102 98-99 -0.1416923297772854 1102 100-101 0.15090763241892802 1102 102-103 0.09846314074982132 1102 104-105 0.0765960815560689 1102 106-107 0.2239279993844292 1102 108-109 0.1540906197312637 1102 110-111 0.24979435120130233 1102 112-113 0.41460507500084987 1102 114-115 0.47620904122609886 1102 116-117 0.35968566239986544 1102 118-119 0.5820807117449718 1102 120-121 0.8204339535879441 1102 122-123 0.7591897332496558 1102 124-125 0.9681454537286562 1103 1 0.8355858630282746 1103 2 0.40948715197614405 1103 3 0.7210275428848867 1103 4 0.8032530949037096 1103 5 0.8777945349329812 1103 6 0.7420107205135835 1103 7 1.0765906885472916 1103 8 1.0951782963488128 1103 9 1.181254955585615 1103 10-11 1.3652921118607821 1103 12-13 1.2360443795626708 1103 14-15 1.3629217195707568 1103 16-17 1.3228223408969697 1103 18-19 1.562460895392448 1103 20-21 1.5819277067190782 1103 22-23 1.4845365520896507 1103 24-25 1.512043297077426 1103 26-27 1.627907475864319 1103 28-29 1.6356491009035352 1103 30-31 1.7136946209898198 1103 32-33 1.657112250959237 1103 34-35 1.6188327219891079 1103 36-37 1.6560406513147328 1103 38-39 1.6162848103334113 1103 40-41 1.5804330711728625 1103 42-43 1.672569776460893 1103 44-45 1.5895327109621107 1103 46-47 1.4104147615402596 1103 48-49 1.2155678129469294 1103 50-51 1.224304884595938 1103 52-53 1.374412040587 1103 54-55 1.3434502144225675 1103 56-57 1.427095004571644 1103 58-59 1.5092930243916527 1103 60-61 1.6002316782921326 1103 62-63 1.4331747568923277 1103 64-65 1.461364932925985 1103 66-67 1.4499906894647019 1103 68-69 1.2838468014878899 1103 70-71 1.1424870986830484 1103 72-73 1.0691621123311634 1103 74-75 1.2057235938916548 1103 76-77 1.3146343842894161 1103 78-79 1.1595339076284716 1103 80-81 1.187239670881052 1103 82-83 1.293906978664186 1103 84-85 1.437340919702521 1103 86-87 1.4749092173664131 1103 88-89 1.3238068452921752 1103 90-91 1.2127827686537707 1103 92-93 1.2374038541479209 1103 94-95 1.2306502286960601 1103 96-97 1.2983005913399346 1103 98-99 1.2034915395437977 1103 100-101 1.1142219764720167 1103 102-103 1.0323142892949981 1103 104-105 1.0610816354100763 1103 106-107 1.094786600711636 1103 108-109 1.343385974504109 1103 110-111 1.3609241112829764 1103 112-113 1.168168321555214 1103 114-115 1.2812352791025674 1103 116-117 1.3302796389184977 1103 118-119 1.2939969955632442 1103 120-121 1.3105758627252584 1103 122-123 1.3402147460112666 1103 124-125 1.5313605634785254 1104 1 0.8838932436643105 1104 2 1.0568784306420547 1104 3 0.6551252575050412 1104 4 0.6087011107082461 1104 5 0.9170420085978819 1104 6 0.9145771344242206 1104 7 1.008754880144565 1104 8 0.7627482711299578 1104 9 1.0823937892967024 1104 10-11 1.1545335884058545 1104 12-13 1.0197210447822869 1104 14-15 1.239989919849279 1104 16-17 1.3139272579935621 1104 18-19 1.3154133484208046 1104 20-21 1.093382463544252 1104 22-23 1.1416465181162891 1104 24-25 1.2208467594661983 1104 26-27 1.1138224983528815 1104 28-29 1.3370160057267881 1104 30-31 1.325613000317837 1104 32-33 1.4487124656690256 1104 34-35 1.6560438832868627 1104 36-37 1.62446446119597 1104 38-39 1.7491069569102464 1104 40-41 1.628096116095037 1104 42-43 1.6464872060514786 1104 44-45 1.5649625754839533 1104 46-47 1.6163518585886223 1104 48-49 1.6273682360211623 1104 50-51 1.5352333651372803 1104 52-53 1.7526907562799288 1104 54-55 1.2216965585654194 1104 56-57 1.2904202566247882 1104 58-59 1.4163975091797347 1104 60-61 1.458783867808343 1104 62-63 1.523419158226293 1104 64-65 1.53614140801756 1104 66-67 1.439694473015365 1104 68-69 1.159920898547341 1104 70-71 0.8894502990660378 1104 72-73 0.9498730792706525 1104 74-75 1.3861050932541588 1104 76-77 1.5700778990298225 1104 78-79 1.5014564062684812 1104 80-81 1.3912097126235885 1104 82-83 1.3996319453318087 1104 84-85 1.4331132726447606 1104 86-87 1.538104673690487 1104 88-89 1.5048098526220777 1104 90-91 1.6439675596483418 1104 92-93 1.6509084910177982 1104 94-95 1.481991068617841 1104 96-97 1.567523423935686 1104 98-99 1.609699551644308 1104 100-101 1.5339041626530552 1104 102-103 1.4072115321933083 1104 104-105 1.5111538716857673 1104 106-107 1.5786721462599544 1104 108-109 1.6193344802668896 1104 110-111 1.5308955134044346 1104 112-113 1.4773349385683119 1104 114-115 1.3767169071516534 1104 116-117 1.377894319971027 1104 118-119 1.3886988690634823 1104 120-121 1.4331144319440163 1104 122-123 1.5901151164398186 1104 124-125 1.950875991037062 1105 1 0.7114912425211948 1105 2 0.4652737422816422 1105 3 0.7491139032882863 1105 4 0.787003951148094 1105 5 0.6037677575549765 1105 6 1.0662723038494306 1105 7 1.1960579211268225 1105 8 1.336502137049905 1105 9 1.3749133026844973 1105 10-11 1.429936921996557 1105 12-13 1.4948317444459462 1105 14-15 1.6877636570500485 1105 16-17 1.6066730504889861 1105 18-19 1.698876167934941 1105 20-21 1.7124888547313972 1105 22-23 1.7118712708592199 1105 24-25 1.6763229049850707 1105 26-27 1.7864808472237854 1105 28-29 1.7294494668007943 1105 30-31 1.667897226752892 1105 32-33 1.7547885948285256 1105 34-35 1.8406527249391402 1105 36-37 1.8573582830373567 1105 38-39 1.870962588291384 1105 40-41 1.7540539481679076 1105 42-43 1.5847528888117388 1105 44-45 1.6148231004056086 1105 46-47 1.5836166869413972 1105 48-49 1.4283986978267507 1105 50-51 1.2405393553924995 1105 52-53 1.28043135174563 1105 54-55 1.332839734377405 1105 56-57 1.3646264259411858 1105 58-59 1.1226524447016573 1105 60-61 1.176071183921529 1105 62-63 1.2210277091303006 1105 64-65 0.9728017322439264 1105 66-67 1.1999395271732816 1105 68-69 1.2233328154550023 1105 70-71 1.2621987518698958 1105 72-73 1.3055778482302571 1105 74-75 1.3127180390182644 1105 76-77 1.523876572290181 1105 78-79 1.457162525767803 1105 80-81 1.3869815040278866 1105 82-83 1.3253117445957407 1105 84-85 1.2744363225099988 1105 86-87 1.43788277746647 1105 88-89 1.4567094886643623 1105 90-91 1.1677387150248784 1105 92-93 1.3110250400473618 1105 94-95 1.4248429804087408 1105 96-97 1.4325670476427028 1105 98-99 1.336150534569665 1105 100-101 1.2819281064481771 1105 102-103 0.8997908260750833 1105 104-105 1.2145519060643934 1105 106-107 1.2135618874328422 1105 108-109 1.2665440740933427 1105 110-111 1.1757239528150194 1105 112-113 1.0841586806488621 1105 114-115 1.1206451481348232 1105 116-117 0.9259303421779776 1105 118-119 1.2486606411448697 1105 120-121 1.2712438376020643 1105 122-123 1.1298079884185945 1105 124-125 1.573652665024671 1106 1 0.04891745127306635 1106 2 -0.22756342894502168 1106 3 0.336741542727097 1106 4 0.1543301645970061 1106 5 0.4235121973712026 1106 6 0.7407941221091363 1106 7 0.7808188186431195 1106 8 0.8959560624578344 1106 9 0.7312352841533922 1106 10-11 0.7448457349310615 1106 12-13 0.796894415326534 1106 14-15 0.9346696765370766 1106 16-17 0.8751001516781614 1106 18-19 0.9142913835522251 1106 20-21 1.0495365905782066 1106 22-23 1.068413135666077 1106 24-25 1.095305572913233 1106 26-27 1.0495264843718886 1106 28-29 1.1085245992856656 1106 30-31 1.1653979893764443 1106 32-33 0.9239268844148825 1106 34-35 1.2175864466298094 1106 36-37 1.2063207537002256 1106 38-39 1.0658939418488202 1106 40-41 1.0677251430893193 1106 42-43 0.7989921673053395 1106 44-45 0.8169667306795247 1106 46-47 0.9187247007599808 1106 48-49 1.0474571377596291 1106 50-51 1.15037608943328 1106 52-53 0.8927572838883897 1106 54-55 0.9620467452492427 1106 56-57 0.38966912511755325 1106 58-59 0.86073112887021 1106 60-61 0.888796828873943 1106 62-63 0.9539534582434115 1106 64-65 0.9593200705659264 1106 66-67 0.7643939919663971 1106 68-69 0.9930010187242431 1106 70-71 1.0505295534478982 1106 72-73 1.1071704281483008 1106 74-75 0.9312897418908399 1106 76-77 0.7521314779855217 1106 78-79 0.821680194116631 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2216 122-123 -4.695133093875462 2216 124-125 -4.822869637006779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54231.0 3 16597.0 4 7979.0 5 6806.0 6 7732.0 7 9211.0 8 9646.0 9 10030.0 10 10702.0 11 11384.0 12 12405.0 13 14423.0 14 16381.0 15 13986.0 16 9423.0 17 8178.0 18 7782.0 19 7392.0 20 7396.0 21 7478.0 22 8415.0 23 10365.0 24 13375.0 25 16907.0 26 20216.0 27 22450.0 28 25390.0 29 29494.0 30 33826.0 31 39775.0 32 47500.0 33 58348.0 34 75818.0 35 107224.0 36 158468.0 37 206776.0 38 65828.0 39 150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63163297762235 19.71393903491869 14.817345412136135 22.837082575322825 2 15.831278525952786 21.671106956192038 38.049512100594626 24.44810241726055 3 19.40382702795407 26.210542864276785 29.844966779796668 24.540663327972478 4 12.867572193341333 17.88378719057292 35.651620742406216 33.597019873679535 5 12.713800150821319 39.435151456047855 33.20296901101063 14.64807938212019 6 30.842455613218135 37.96897317919405 16.367980482342386 14.820590725245422 7 26.668538650439817 34.54482630484696 20.526133930350436 18.260501114362796 8 26.590490058675215 35.05211324089122 18.92832611278185 19.429070587651722 9 26.590356887637146 16.017655218798605 19.476732943797554 37.9152549497667 10-11 25.003163142932443 27.382266979351925 26.1015408814681 21.513028996247524 12-13 26.214326007766374 24.89035189119343 27.33989526577424 21.55542683526596 14-15 23.012451171156627 25.550437100379032 26.42371308963994 25.013398638824402 16-17 21.4903088311328 28.523226438332554 27.1863873861377 22.800077344396946 18-19 21.458125519403463 25.885996693472823 31.14924308439379 21.506634702729922 20-21 22.23826572154342 24.941266080105123 31.478396552427146 21.342071645924314 22-23 21.903223826742117 24.863323432706704 31.619723460617898 21.613729279933285 24-25 21.67909359244784 25.165470492741832 31.56058031739733 21.594855597413 26-27 21.50624596990131 25.349751615612444 31.611946999000413 21.532055415485836 28-29 21.312296813668414 25.2809404390296 31.795849807522064 21.610912939779922 30-31 21.582497328680347 25.115364858968615 31.56032810783136 21.741809704519678 32-33 21.389094626507056 25.21692124420023 31.34641236095897 22.047571768333743 34-35 21.736975687838537 25.261099953173087 31.44405949791801 21.55786486107036 36-37 22.055011950529934 24.926417859127508 31.198407380660736 21.82016280968182 38-39 21.755765300503494 25.19064941441142 31.033462324514687 22.020122960570397 40-41 21.679471906796795 25.20948106200404 31.372389946254142 21.738657084945025 42-43 21.824912756507636 25.168623112316485 30.86725622053877 22.139207910637108 44-45 21.84563597584505 25.376696004243847 30.65039802873003 22.127269991181073 46-47 22.205539026487887 25.67295817440628 29.82655548148067 22.294947317625162 48-49 22.11100247417584 25.929581407783353 29.419783486494598 22.539632631546205 50-51 22.1722987188169 26.105382448644853 29.25552328672919 22.466795545809052 52-53 22.388769276166954 25.92281378442976 29.185690974344404 22.502725965058886 54-55 22.15749310417012 26.088515469273727 29.09594640378583 22.65804502277032 56-57 22.152448421429646 26.268434730481527 28.834394513531624 22.744722334557203 58-59 22.21807942045239 25.81393794512642 29.006931182924966 22.96105145149622 60-61 22.210036763747734 26.038031521151556 29.119275788638294 22.632655926462416 62-63 22.089774835706486 26.041436350292184 29.289349105959122 22.579439708042205 64-65 22.006339721114482 26.03106639893164 29.17309977557505 22.78949410437883 66-67 21.99187548918147 26.217856941689988 29.121755849370356 22.66851171975818 68-69 22.36486080338028 25.9427912801028 29.100834687909447 22.59151322860747 70-71 22.139964539335026 26.048876532488375 28.99649176493732 22.81466716323928 72-73 22.156147986484932 26.130634466791147 28.84638503825599 22.866832508467937 74-75 22.13341538647788 26.444358131710427 28.77715593376226 22.645070548049432 76-77 22.192466163984978 26.08010848374131 28.932934954312238 22.794490397961475 78-79 22.17397079581366 26.272334207940062 28.82700693660376 22.72668805964252 80-81 22.17596508071554 26.273281064258008 28.812318934396874 22.738434920629583 82-83 22.13723226903699 26.336395437697092 28.665550779453664 22.860821513812258 84-85 22.16964119826446 26.389233341768342 28.55159409056173 22.889531369405468 86-87 22.224664918574142 26.222985202864763 28.541589777778153 23.010760100782942 88-89 22.197766998984005 26.322358047727757 28.344341686735035 23.1355332665532 90-91 22.041770948316376 26.492345019323455 28.504809216073816 22.96107481628635 92-93 21.95098882815656 26.368064257541317 28.684318602462373 22.99662831183975 94-95 21.841180273512865 26.202177913672024 28.80998279090062 23.14665902191449 96-97 22.15611454447849 25.973114778632233 28.47069862311715 23.400072053772124 98-99 21.960327025233102 26.10678301180504 28.314737195652274 23.61815276730958 100-101 21.829169201072585 26.095588473290686 28.516301677184714 23.558940648452012 102-103 21.87018437359971 25.871320998043696 28.657606178125526 23.600888450231068 104-105 21.672241899238916 26.015921149201294 28.488499664140924 23.823337287418862 106-107 21.74110610794069 26.119954467039047 28.422496443788315 23.716442981231943 108-109 21.739035399293982 26.206885825570183 28.168067410572796 23.886011364563043 110-111 21.759440632340354 26.134649756281288 28.1973053907318 23.908604220646566 112-113 21.780860152317764 26.231728467818478 28.309136627806776 23.67827475205698 114-115 21.58359023679956 26.544930713828734 28.0476373428209 23.82384170655081 116-117 21.45357620554071 26.581879415243716 28.085216568150805 23.879327811064773 118-119 21.38674067055798 26.56968928622171 27.941709325112424 24.10186071810789 120-121 21.312977651149094 26.689152692417146 27.970847753155898 24.027021903277863 122-123 21.320274939224912 26.600214473109517 27.82954642426413 24.249964163401437 124-125 21.129869583508622 26.58502313840976 27.878796802692467 24.406310475389144 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2112.0 1 1534.5 2 1634.5 3 2176.0 4 1357.5 5 842.5 6 1817.5 7 4525.0 8 6945.0 9 7080.5 10 4895.0 11 2430.5 12 1628.0 13 1493.5 14 1502.0 15 1534.5 16 1608.0 17 1753.5 18 1946.0 19 2055.5 20 2266.0 21 2610.5 22 3022.5 23 3642.5 24 4238.0 25 5158.5 26 6743.5 27 8413.0 28 9974.5 29 11937.5 30 14178.5 31 16892.0 32 20271.0 33 22196.0 34 22923.5 35 24127.5 36 26079.5 37 27962.0 38 28978.5 39 29721.5 40 30573.5 41 31823.5 42 32410.5 43 33591.0 44 35464.0 45 36973.5 46 37653.0 47 38136.5 48 40296.5 49 40796.5 50 40755.0 51 40268.0 52 38821.0 53 38950.0 54 38665.0 55 36973.0 56 35108.0 57 33232.0 58 32254.0 59 30029.5 60 25295.0 61 21077.5 62 17803.0 63 13655.0 64 9983.5 65 8147.5 66 6449.5 67 5064.0 68 4000.5 69 3027.5 70 2272.0 71 1912.0 72 1623.0 73 1400.5 74 1104.5 75 840.0 76 627.0 77 413.0 78 285.5 79 191.5 80 130.5 81 82.0 82 59.0 83 45.0 84 26.0 85 16.0 86 11.5 87 7.0 88 4.0 89 3.0 90 2.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08987067534155481 2 0.0 3 0.0 4 0.09087951360544504 5 0.0 6 0.0 7 0.0015132573958353476 8 0.004876051608802786 9 0.003110584646994881 10-11 4.623842042830228E-4 12-13 0.0 14-15 4.203492766209299E-5 16-17 1.6813971064837195E-4 18-19 0.0018075018894699985 20-21 0.0010508731915523247 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.203492766209299E-5 52-53 0.0 54-55 0.0 56-57 0.007398147268528365 58-59 6.305239149313949E-4 60-61 0.0 62-63 0.0 64-65 2.101746383104649E-4 66-67 0.0 68-69 4.203492766209299E-5 70-71 0.0 72-73 0.0 74-75 0.0011769779745386035 76-77 0.0 78-79 0.0 80-81 8.406985532418597E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.668033362281387E-4 90-91 0.0 92-93 0.0019756416001183706 94-95 0.0 96-97 0.008196810894108132 98-99 0.003152619574656974 100-101 0.0019756416001183706 102-103 0.0 104-105 0.0 106-107 0.0015973272511595334 108-109 0.0 110-111 8.406985532418597E-5 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 6.305239149313949E-4 122-123 0.005086226247113251 124-125 0.08297694720497156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1189487.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.130805237691895 #Duplication Level Percentage of deduplicated Percentage of total 1 73.00212839417489 35.136512236770095 2 12.270194545443692 11.811486877906798 3 5.3409057089236 7.711862774072356 4 2.80290968218472 5.396252000482947 5 1.7150761272984236 4.127399752540764 6 1.1143902497277969 3.2181900041058857 7 0.7527888753645406 2.5362634321690276 8 0.541987897778787 2.0869051159341483 9 0.4300405667874724 1.8628378887919022 >10 1.8912969784975129 15.545338884261062 >50 0.09103351408091191 2.963114687148396 >100 0.04354523600335318 3.8103194591233023 >500 0.002291852787972638 0.7585845833323599 >1k 8.814818415279378E-4 0.8723722708322138 >5k 5.288891049167626E-4 2.1625600325289174 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9976 0.8386808767140793 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8346 0.7016470125356561 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7164 0.6022764435424683 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2486 0.20899766033592634 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2255 0.18957752375603937 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1928 0.16208668106503055 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1812 0.152334577847425 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1800 0.15132573958353474 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.203492766209299E-5 0.0 12-13 0.0 0.0 0.0 1.2610478298627896E-4 0.0 14-15 0.0 0.0 0.0 1.6813971064837195E-4 0.0 16-17 0.0 0.0 0.0 3.783143489588369E-4 0.0 18-19 8.406985532418597E-5 0.0 0.0 5.884889872693018E-4 0.0 20-21 1.6813971064837195E-4 0.0 0.0 7.566286979176737E-4 0.0 22-23 1.6813971064837195E-4 0.0 0.0 0.0018495368171320913 0.0 24-25 1.6813971064837195E-4 0.0 0.0 0.0034888989959537178 0.0 26-27 1.6813971064837195E-4 0.0 0.0 0.004539772187506043 0.0 28-29 1.6813971064837195E-4 0.0 0.0 0.005968959728017204 0.0 30-31 1.6813971064837195E-4 0.0 0.0 0.010634836698509526 0.0 32-33 1.6813971064837195E-4 0.0 0.0 0.015847167728609057 0.0 34-35 1.6813971064837195E-4 0.0 0.0 0.023581594418434167 0.0 36-37 1.6813971064837195E-4 0.0 0.0 0.03581375836810323 0.0 38-39 1.6813971064837195E-4 0.0 0.0 0.05805023510135041 0.0 40-41 1.6813971064837195E-4 0.0 0.0 0.08398578546886179 0.0 42-43 1.6813971064837195E-4 0.0 0.0 0.10979523105338687 0.0 44-45 1.6813971064837195E-4 0.0 0.0 0.13871526128490685 0.0 46-47 2.1017463831046493E-4 0.0 0.0 0.17347814646145776 0.0 48-49 2.522095659725579E-4 0.0 0.0 0.2158493535448475 0.0 50-51 2.522095659725579E-4 0.0 0.0 0.26221387875613605 0.0 52-53 2.942444936346509E-4 0.0 0.0 0.3094611374483286 0.0 54-55 3.362794212967439E-4 0.0 0.0 0.35708671048947993 0.0 56-57 4.2034927662092987E-4 0.0 0.0 0.41328740877369824 0.0 58-59 4.2034927662092987E-4 0.0 0.0 0.47810526722864566 0.0 60-61 4.2034927662092987E-4 0.0 0.0 0.5550712197779379 0.0 62-63 4.2034927662092987E-4 0.0 0.0 0.6298933910164635 0.0 64-65 4.2034927662092987E-4 0.0 0.0 0.7041270732677196 0.0 66-67 4.2034927662092987E-4 0.0 0.0 0.7960994949923791 0.0 68-69 4.2034927662092987E-4 0.0 0.0 0.9019014079178671 0.0 70-71 4.2034927662092987E-4 0.0 0.0 1.0227938598740465 0.0 72-73 4.2034927662092987E-4 0.0 0.0 1.1497813763412295 0.0 74-75 4.2034927662092987E-4 0.0 0.0 1.2749193559912801 0.0 76-77 4.2034927662092987E-4 0.0 0.0 1.4174177607657756 0.0 78-79 4.2034927662092987E-4 0.0 0.0 1.5814380485032622 0.0 80-81 4.2034927662092987E-4 0.0 0.0 1.7700487689230735 0.0 82-83 4.623842042830228E-4 0.0 0.0 1.965132868202847 0.0 84-85 5.044191319451158E-4 0.0 0.0 2.162192609082739 0.0 86-87 5.044191319451158E-4 0.0 0.0 2.364296541282082 0.0 88-89 5.044191319451158E-4 0.0 0.0 2.6003226601047342 0.0 90-91 5.044191319451158E-4 0.0 0.0 2.874516493244567 0.0 92-93 5.044191319451158E-4 0.0 0.0 3.1735109337050345 0.0 94-95 5.044191319451158E-4 0.0 0.0 3.477675670267939 0.0 96-97 5.044191319451158E-4 0.0 0.0 3.785791690031081 0.0 98-99 5.044191319451158E-4 0.0 0.0 4.107989410561023 0.0 100-101 5.464540596072087E-4 0.0 0.0 4.486471899230509 0.0 102-103 6.305239149313948E-4 0.0 0.0 4.9081242586089635 0.0 104-105 6.725588425934878E-4 0.0 0.0 5.325404985510561 0.0 106-107 7.566286979176737E-4 0.0 0.0 5.7422653631355365 0.0 108-109 7.986636255797667E-4 0.0 0.0 6.18699489780048 0.0 110-111 8.406985532418597E-4 0.0 0.0 6.689648562783788 0.0 112-113 8.406985532418597E-4 0.0 0.0 7.224164702934963 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4850 0.0 50.87429 1 GTATCAA 7880 0.0 45.228764 1 TATCAAC 10485 0.0 33.845203 2 ATCAACG 10760 0.0 33.588898 3 TCAACGC 10775 0.0 33.37636 4 CAACGCA 11050 0.0 32.46726 5 AACGCAG 11140 0.0 32.41859 6 ACGCAGA 12425 0.0 28.778534 7 CGCAGAG 12440 0.0 28.696007 8 GTACATG 6270 0.0 28.516317 1 TACATGG 6415 0.0 27.473522 2 GCGTATC 110 7.6763873E-4 27.090502 1 GCAGAGT 12920 0.0 26.801004 9 GAGTACT 8435 0.0 26.591747 12-13 AGTACTT 8675 0.0 26.507612 12-13 TAAGGTG 595 0.0 25.998423 5 CATGGGG 2700 0.0 24.919195 4 GTACTTT 8950 0.0 24.762463 14-15 ACATGGG 6730 0.0 24.595125 3 AGAGTAC 12320 0.0 24.122114 10-11 >>END_MODULE