FastQCFastQC Report
Thu 26 May 2016
SRR1364526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099461
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22640.10783720202471014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.057.261511
GTATCAA42850.052.767391
ATCAACG50850.043.1765173
TCAACGC52050.042.181094
CAACGCA53150.041.6439485
AACGCAG53300.041.408216
TATCAAC57700.038.4622652
GTACATG79050.036.883071
ACGCAGA64000.034.3931437
TACATGG80700.034.283192
CGCAGAG66550.032.183678
ACATGGG83750.032.1118853
GCAGAGT75650.027.2911859
GAGTACA62450.026.0113621
CATGGGA47250.025.1849084
AGTACAT61300.024.9444832
CATGGGG46850.022.986944
AGAGTAC72700.022.30521210-11
CAGAGTA76650.021.8544810-11
GAGTACT38250.021.54882612-13
Produced by FastQC (version 0.11.5)