##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364526_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2099461 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.292442203022585 31.0 28.0 33.0 16.0 34.0 2 28.212238760329438 31.0 28.0 34.0 16.0 34.0 3 28.430246144129374 31.0 28.0 34.0 16.0 34.0 4 31.785445883491047 35.0 32.0 37.0 18.0 37.0 5 31.86974799722405 35.0 32.0 37.0 19.0 37.0 6 31.860042172729095 35.0 32.0 37.0 19.0 37.0 7 31.69580192249344 35.0 32.0 37.0 17.0 37.0 8 31.6009356687264 35.0 32.0 37.0 17.0 37.0 9 32.72522185456172 37.0 32.0 39.0 16.0 39.0 10-11 32.63676677013767 37.0 32.0 39.0 14.0 39.0 12-13 32.543176081861006 37.0 32.0 39.0 12.0 39.0 14-15 33.26617379413097 38.0 32.0 41.0 10.5 41.0 16-17 33.36073449328185 38.0 32.0 41.0 10.0 41.0 18-19 33.147375921724674 38.0 32.0 41.0 10.0 41.0 20-21 32.86015672594061 38.0 31.5 40.0 10.0 41.0 22-23 32.74787814586696 38.0 31.0 40.0 9.5 41.0 24-25 32.561807292443156 38.0 31.0 40.0 8.0 41.0 26-27 32.457133045100626 38.0 30.5 40.0 8.0 41.0 28-29 32.512585849415636 38.0 31.0 40.0 8.0 41.0 30-31 32.462401540204844 38.0 30.5 40.0 8.0 41.0 32-33 32.445129726153525 38.0 31.0 40.0 4.5 41.0 34-35 31.985568915069152 38.0 30.0 40.0 2.0 41.0 36-37 31.84304852531197 38.0 30.0 40.0 2.0 41.0 38-39 31.63538165271944 37.0 30.0 40.0 2.0 41.0 40-41 31.83377995590297 37.5 30.0 40.0 2.0 41.0 42-43 31.71330855872055 37.0 30.0 40.0 2.0 41.0 44-45 31.559590771155072 37.0 30.0 40.0 2.0 41.0 46-47 31.516402305163087 37.0 30.0 40.0 2.0 41.0 48-49 31.372731858319824 37.0 30.0 40.0 2.0 41.0 50-51 31.21194654246971 37.0 30.0 40.0 2.0 41.0 52-53 31.14152989743558 37.0 29.5 40.0 2.0 41.0 54-55 31.164496268327916 37.0 30.0 40.0 2.0 41.0 56-57 31.02848635911789 36.5 29.5 40.0 2.0 41.0 58-59 30.823659977489463 36.0 29.0 40.0 2.0 41.0 60-61 30.603500374619962 36.0 29.0 40.0 2.0 41.0 62-63 30.312744318660837 35.0 28.5 39.0 2.0 41.0 64-65 30.01537537491766 35.0 28.0 39.0 2.0 41.0 66-67 29.76414994134209 35.0 28.0 39.0 2.0 40.5 68-69 29.72879848684972 35.0 29.0 38.5 2.0 40.0 70-71 29.491940312299207 35.0 28.5 37.5 2.0 40.0 72-73 29.213196863385413 35.0 28.0 37.0 2.0 40.0 74-75 28.917854868463856 34.5 28.0 37.0 2.0 39.0 76-77 28.611799171311112 34.0 28.0 36.0 2.0 39.0 78-79 28.31664103310326 34.0 28.0 36.0 2.0 38.5 80-81 28.03133804343115 34.0 27.0 35.0 2.0 37.0 82-83 27.80301015355846 34.0 27.0 35.0 2.0 37.0 84-85 27.5736705754477 34.0 26.5 35.0 2.0 36.5 86-87 27.386982897038813 34.0 26.0 35.0 2.0 36.0 88-89 27.203173100143324 34.0 26.0 35.0 2.0 36.0 90-91 27.07288918441448 34.0 26.0 35.0 2.0 35.5 92-93 26.954469742471996 34.0 26.0 35.0 2.0 35.0 94-95 26.833537512723503 34.0 25.5 35.0 2.0 35.0 96-97 26.721407542221552 34.0 25.0 35.0 2.0 35.0 98-99 26.637963267714902 34.0 25.0 35.0 2.0 35.0 100-101 26.547915155366066 33.5 25.0 35.0 2.0 35.0 102-103 25.371592280113802 31.5 23.5 34.0 2.0 35.0 104-105 26.252088512241954 33.0 24.5 35.0 2.0 35.0 106-107 26.40362907431955 33.0 25.0 35.0 2.0 35.0 108-109 26.362407779901602 34.0 25.0 35.0 2.0 35.0 110-111 26.27517110344036 33.0 24.5 35.0 2.0 35.0 112-113 26.186407606523773 33.0 24.0 35.0 2.0 35.0 114-115 26.06426411350342 33.0 24.0 35.0 2.0 35.0 116-117 25.95634617647101 33.0 23.5 35.0 2.0 35.0 118-119 25.80678231222204 33.0 23.0 35.0 2.0 35.0 120-121 25.630499209082714 33.0 21.5 35.0 2.0 35.0 122-123 25.388632844334808 33.0 18.5 35.0 2.0 35.0 124-125 23.951304168069804 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7615609176582758 1101 2 0.7171112871986338 1101 3 0.6711885281840999 1101 4 0.8559740396677284 1101 5 0.8706629516127506 1101 6 0.8369010595518667 1101 7 0.8461722556368905 1101 8 0.8540485529453363 1101 9 1.2697543820436081 1101 10-11 1.3036398125775008 1101 12-13 1.088354180960188 1101 14-15 1.2990040185970173 1101 16-17 1.0789879112453207 1101 18-19 1.5320675359010352 1101 20-21 1.8126375536333796 1101 22-23 1.7281843722222732 1101 24-25 1.7927692030836226 1101 26-27 1.961299307198928 1101 28-29 1.823811034057215 1101 30-31 1.875744366822758 1101 32-33 1.7552908517663184 1101 34-35 2.130275758250434 1101 36-37 1.8808437517230416 1101 38-39 1.7375083474495305 1101 40-41 1.6084064820480606 1101 42-43 1.598990617381702 1101 44-45 1.5580167746253721 1101 46-47 1.3870907373051757 1101 48-49 1.3246705250170692 1101 50-51 1.406915238845901 1101 52-53 1.1307618373845116 1101 54-55 1.140707156254809 1101 56-57 1.1641552968475182 1101 58-59 1.1145661224894319 1101 60-61 1.162328502228501 1101 62-63 1.0106474448281801 1101 64-65 1.0975132976381623 1101 66-67 1.095126988732396 1101 68-69 0.9312011656533343 1101 70-71 0.7388678025610389 1101 72-73 0.7183548130729029 1101 74-75 0.7016408650445705 1101 76-77 0.8415829780621422 1101 78-79 0.8715745926245191 1101 80-81 0.9603116333629735 1101 82-83 0.8317597352888377 1101 84-85 0.8710013496217499 1101 86-87 0.9451774505834791 1101 88-89 0.866140097147035 1101 90-91 0.8773101971917292 1101 92-93 0.7246935302562569 1101 94-95 0.813817456486003 1101 96-97 0.683380302366217 1101 98-99 0.808199343307983 1101 100-101 0.877581010828905 1101 102-103 0.8011432826363496 1101 104-105 0.8467964385662832 1101 106-107 0.848181657753269 1101 108-109 0.7918425723396254 1101 110-111 0.6721584966738554 1101 112-113 0.6785715030981407 1101 114-115 0.8923082963328319 1101 116-117 1.0135828099628732 1101 118-119 0.9478124447764031 1101 120-121 0.937043502365956 1101 122-123 1.1380057072829608 1101 124-125 1.368861178915541 1102 1 0.15356291603342598 1102 2 0.3149767813983182 1102 3 0.41641076798952525 1102 4 0.6365302690197652 1102 5 0.648582413346773 1102 6 -0.24845921314935637 1102 7 -0.7129469195791849 1102 8 0.23016854017952326 1102 9 0.39857483357618406 1102 10-11 0.5116300952441222 1102 12-13 0.65066760976827 1102 14-15 0.8239689421436935 1102 16-17 0.7523271997854835 1102 18-19 0.7779840236976412 1102 20-21 1.193692633390178 1102 22-23 1.0959096359652278 1102 24-25 1.241975504767801 1102 26-27 0.8430410483718731 1102 28-29 1.3386085213835628 1102 30-31 1.2330372243016683 1102 32-33 1.0803869976029006 1102 34-35 1.3839582003088537 1102 36-37 1.3558030642591845 1102 38-39 1.538941609316307 1102 40-41 1.3332556619545741 1102 42-43 1.271460453872173 1102 44-45 1.2416353122636465 1102 46-47 1.0015793339191355 1102 48-49 0.5057284153707968 1102 50-51 1.0166923686928335 1102 52-53 0.8381929538289654 1102 54-55 0.6883760003194332 1102 56-57 0.6348380213913032 1102 58-59 0.8710011674559297 1102 60-61 0.7667020048982636 1102 62-63 0.5890669178656935 1102 64-65 0.6582678634834203 1102 66-67 0.6666483811428492 1102 68-69 0.5761337714086636 1102 70-71 0.5587924500500705 1102 72-73 0.7958737240113969 1102 74-75 0.4534302275054678 1102 76-77 0.6412325801284595 1102 78-79 0.5584818341682372 1102 80-81 0.21054147094509545 1102 82-83 0.5411742064821503 1102 84-85 0.1416266504116308 1102 86-87 0.6133908620595534 1102 88-89 0.786643788703163 1102 90-91 0.19725775715571459 1102 92-93 0.6633688002008533 1102 94-95 0.556815862388742 1102 96-97 0.7130108677300058 1102 98-99 0.7294131179213252 1102 100-101 0.8334300463359092 1102 102-103 0.6319124045223674 1102 104-105 0.8635087468830633 1102 106-107 0.7959602067479814 1102 108-109 0.319486677041283 1102 110-111 0.5259899411027504 1102 112-113 0.297413775453542 1102 114-115 0.43939058103856254 1102 116-117 0.3992650385788643 1102 118-119 0.6354820607368481 1102 120-121 0.9157068965202022 1102 122-123 0.8442761795793352 1102 124-125 1.109421493816452 1103 1 0.8552922126194638 1103 2 0.6907128017638868 1103 3 0.6488514545010524 1103 4 0.7600676300086207 1103 5 0.8399851086442638 1103 6 0.813399041974062 1103 7 0.8875067392396296 1103 8 1.006579211441668 1103 9 1.2691005446974941 1103 10-11 1.3929376678646577 1103 12-13 1.254158902329138 1103 14-15 1.6680168268600042 1103 16-17 1.392735275759641 1103 18-19 1.4853264570694122 1103 20-21 1.8341419322904855 1103 22-23 1.8445842000766959 1103 24-25 1.9763771731081832 1103 26-27 1.8023581012322651 1103 28-29 1.6832171515426353 1103 30-31 1.6714475380918188 1103 32-33 1.6390739285922038 1103 34-35 2.036332341239607 1103 36-37 1.9854430197094999 1103 38-39 1.987858758466846 1103 40-41 1.707511996234281 1103 42-43 1.7264462583081297 1103 44-45 1.4077390122020645 1103 46-47 1.4429677716632163 1103 48-49 1.3279074344511201 1103 50-51 1.3843713733089693 1103 52-53 1.4089377456893502 1103 54-55 1.2541154610946244 1103 56-57 1.2926700674122102 1103 58-59 1.2718284155001633 1103 60-61 1.179937198739001 1103 62-63 1.2077664740218594 1103 64-65 1.3295421478162304 1103 66-67 1.3988618766403533 1103 68-69 1.3707807316569536 1103 70-71 1.2727689333522 1103 72-73 1.3202972745967863 1103 74-75 1.3583730009922022 1103 76-77 1.4205088646513673 1103 78-79 1.3928465155222476 1103 80-81 1.2904886353450173 1103 82-83 1.2215658346120968 1103 84-85 1.2263104220097567 1103 86-87 1.1859657865358457 1103 88-89 1.2592580247669254 1103 90-91 1.2812481260750737 1103 92-93 1.369823804188833 1103 94-95 1.208713201805299 1103 96-97 1.34912649028918 1103 98-99 1.3132630292765413 1103 100-101 1.2235948140617658 1103 102-103 1.1154229757750045 1103 104-105 1.1882780947962956 1103 106-107 1.2040717214787193 1103 108-109 1.3808141999050285 1103 110-111 1.3606756756973617 1103 112-113 1.4098212563298205 1103 114-115 1.5314454560886688 1103 116-117 1.466120772801542 1103 118-119 1.4923956826926528 1103 120-121 1.4820238398660557 1103 122-123 1.5660377003023775 1103 124-125 1.5452099158565638 1104 1 0.725856008534663 1104 2 0.7871075365623668 1104 3 0.7239465853545113 1104 4 0.6790433231048425 1104 5 0.6983294435048677 1104 6 0.6009873777665611 1104 7 0.7594470203610051 1104 8 0.7824839252725297 1104 9 1.0036272201653773 1104 10-11 0.8820354779135826 1104 12-13 0.8371877522165292 1104 14-15 0.8643591426167774 1104 16-17 0.9416189443159766 1104 18-19 1.2594741622802559 1104 20-21 1.404431984321235 1104 22-23 1.172166323242024 1104 24-25 1.574485233991929 1104 26-27 1.5971870598406355 1104 28-29 1.4884211975160753 1104 30-31 1.4464446259228367 1104 32-33 1.4956699913397387 1104 34-35 1.7556992357026076 1104 36-37 1.7836190312028535 1104 38-39 1.7950984105597279 1104 40-41 1.4011421119444663 1104 42-43 1.4536983356553392 1104 44-45 1.3781047806263942 1104 46-47 1.2584973981290126 1104 48-49 1.198087794783497 1104 50-51 1.0697979089927507 1104 52-53 1.1632642580757775 1104 54-55 0.9699683383175817 1104 56-57 1.0051011460795962 1104 58-59 0.8548019341768729 1104 60-61 0.9234731543187173 1104 62-63 1.007637367863591 1104 64-65 1.015475400903128 1104 66-67 0.9652302077733808 1104 68-69 0.943098624023186 1104 70-71 0.9208303995322105 1104 72-73 0.7894040152972579 1104 74-75 0.8712942947718112 1104 76-77 0.9151164984169995 1104 78-79 1.0128133167958389 1104 80-81 0.8843420173439291 1104 82-83 0.8827699194144607 1104 84-85 0.9134464008868264 1104 86-87 0.9689829877473102 1104 88-89 1.012108067478735 1104 90-91 0.9583186782223159 1104 92-93 0.9744065874458059 1104 94-95 1.0428117884326191 1104 96-97 0.859627771643531 1104 98-99 0.995322126892443 1104 100-101 1.023841377702368 1104 102-103 1.02158415462101 1104 104-105 1.0493142445206125 1104 106-107 1.04080582103607 1104 108-109 1.0862269513222813 1104 110-111 1.0685870681024277 1104 112-113 1.1448061130511036 1104 114-115 1.1000746010758569 1104 116-117 1.1300933595841762 1104 118-119 1.0553965757578112 1104 120-121 1.0801386958107635 1104 122-123 1.1442381740151966 1104 124-125 1.4658727255171975 1105 1 0.3152878206692833 1105 2 0.5678279963429667 1105 3 0.5997408244130575 1105 4 0.703755770174574 1105 5 0.6467440062647682 1105 6 0.6185929152035605 1105 7 0.8033299161881651 1105 8 0.7047036134305955 1105 9 0.7028362120386333 1105 10-11 0.9649054576157283 1105 12-13 0.8692203504178551 1105 14-15 0.9642781792669481 1105 16-17 0.9030900825922572 1105 18-19 1.0196048439688497 1105 20-21 1.4060189419998324 1105 22-23 1.633872939430134 1105 24-25 1.6539874424288357 1105 26-27 1.34637818656158 1105 28-29 1.104393213845583 1105 30-31 1.0309065047286836 1105 32-33 1.0979407662312184 1105 34-35 1.6935667066812385 1105 36-37 1.6318422087672673 1105 38-39 1.6922295331754889 1105 40-41 1.376947944346533 1105 42-43 0.8370387010167022 1105 44-45 1.1264957765896284 1105 46-47 1.0825235572733085 1105 48-49 1.057652113132903 1105 50-51 1.0357758170214275 1105 52-53 1.0687986541956178 1105 54-55 0.7991162845852884 1105 56-57 0.8206369866160088 1105 58-59 0.7682830921428341 1105 60-61 0.7578772564617715 1105 62-63 0.737522916855923 1105 64-65 0.9306873880402193 1105 66-67 0.7510072658769396 1105 68-69 0.4979165135025134 1105 70-71 0.7795727308205542 1105 72-73 0.8100791279997637 1105 74-75 0.8485415028666239 1105 76-77 0.9311799311109858 1105 78-79 0.9878410647983635 1105 80-81 0.8536033123013986 1105 82-83 0.882401403134363 1105 84-85 0.7917822429436754 1105 86-87 0.835238565856038 1105 88-89 0.9827518211372883 1105 90-91 0.8759042599040718 1105 92-93 0.8845013487749753 1105 94-95 0.9445340651372227 1105 96-97 1.0547458336926248 1105 98-99 0.83636978503705 1105 100-101 0.9437083773450645 1105 102-103 0.8237866998350505 1105 104-105 0.918992139625562 1105 106-107 0.9174769729962939 1105 108-109 0.881935323616851 1105 110-111 1.0076824832201439 1105 112-113 1.003301563138912 1105 114-115 0.9475597958060185 1105 116-117 1.00659612298114 1105 118-119 1.0567948090082808 1105 120-121 0.8956796678270926 1105 122-123 1.101674258581987 1105 124-125 1.2242450200869506 1106 1 0.5566243404072893 1106 2 0.8331811561942679 1106 3 0.5709209742920933 1106 4 0.7410561773262856 1106 5 0.6878834409729748 1106 6 0.7541756142063356 1106 7 0.5232360942727787 1106 8 0.720755422010285 1106 9 0.9187971490776405 1106 10-11 1.102129450062769 1106 12-13 0.9426454404042843 1106 14-15 1.3491731459277148 1106 16-17 1.1477714421249203 1106 18-19 1.2079384723396558 1106 20-21 1.5412325491279404 1106 22-23 1.5393169708221208 1106 24-25 1.737953926172274 1106 26-27 1.8348932629762942 1106 28-29 1.7576309550387137 1106 30-31 1.5867494267797824 1106 32-33 1.4820420820983315 1106 34-35 1.7522962753364482 1106 36-37 1.6538381372501831 1106 38-39 1.9359349087581812 1106 40-41 1.525568054100468 1106 42-43 1.4636735036366417 1106 44-45 1.4069805231524413 1106 46-47 1.1226462928607255 1106 48-49 1.1984855939849766 1106 50-51 1.2031653254889996 1106 52-53 1.1158550653598311 1106 54-55 1.052506560150288 1106 56-57 1.1606216446675788 1106 58-59 1.0173206799164767 1106 60-61 1.0115903030186892 1106 62-63 0.9242718989766487 1106 64-65 1.002474134957076 1106 66-67 0.8185012176088087 1106 68-69 0.8208837053358771 1106 70-71 0.9648108260586525 1106 72-73 0.9601611484163541 1106 74-75 1.0491309877311998 1106 76-77 0.9213050269981622 1106 78-79 1.0105896338666334 1106 80-81 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0.48147332721786995 2201 86-87 0.6086716295174597 2201 88-89 0.6123147480316042 2201 90-91 0.5728452194578857 2201 92-93 0.5644791864798435 2201 94-95 0.5715856292572958 2201 96-97 0.7617310948467662 2201 98-99 0.7619177500831569 2201 100-101 0.7642518363643589 2201 102-103 0.8259310858084348 2201 104-105 0.7144573179239835 2201 106-107 0.6036658283965153 2201 108-109 0.7397250599912937 2201 110-111 0.695194983996057 2201 112-113 0.5773374803107814 2201 114-115 0.6094451312176368 2201 116-117 0.6044007568319749 2201 118-119 0.5371369796717431 2201 120-121 0.47699362294614644 2201 122-123 0.542503631899411 2201 124-125 0.06670248296395798 2202 1 0.5283666086058219 2202 2 0.8612426749382607 2202 3 0.7209514386833398 2202 4 0.8119562246249217 2202 5 0.6681911364104351 2202 6 0.6336938801263905 2202 7 0.4333686830340149 2202 8 0.3318130297164039 2202 9 -0.596255820310482 2202 10-11 0.6275807451808362 2202 12-13 0.29714420199372427 2202 14-15 0.7878999988391229 2202 16-17 0.812863216739288 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2216 100-101 -3.1067103629483555 2216 102-103 -2.965651901650844 2216 104-105 -3.15681824009679 2216 106-107 -3.2088605504774357 2216 108-109 -3.2957345221545893 2216 110-111 -3.3780624164336572 2216 112-113 -3.3260715309038247 2216 114-115 -3.487481530731113 2216 116-117 -3.4117086860079517 2216 118-119 -3.575667692337639 2216 120-121 -3.695293417455769 2216 122-123 -3.8395753102678967 2216 124-125 -3.865041104817923 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 140238.0 3 23989.0 4 14975.0 5 11747.0 6 10736.0 7 11403.0 8 12063.0 9 12582.0 10 13173.0 11 13995.0 12 15066.0 13 16135.0 14 17051.0 15 15432.0 16 12310.0 17 11248.0 18 10221.0 19 9763.0 20 9940.0 21 10689.0 22 12643.0 23 16789.0 24 22422.0 25 28477.0 26 34444.0 27 39702.0 28 46138.0 29 53571.0 30 61109.0 31 70153.0 32 86363.0 33 107829.0 34 139781.0 35 190308.0 36 291935.0 37 379171.0 38 125386.0 39 484.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.97444252306842 18.20173495491118 12.71585706867887 24.107965453341524 2 15.20007240284562 19.634603796862386 40.64805310177125 24.517270698520743 3 19.425418270176596 24.523162709873432 28.235493623830028 27.815925396119944 4 12.38440205363497 15.915956827555064 37.07527549633164 34.62436562247832 5 13.838003930209016 37.939760614541775 33.82490323349015 14.397332221759065 6 32.7116158351723 36.13093505784602 17.287502982919836 13.869946124061839 7 29.432993201977624 31.703097289698835 20.550929910965007 18.31297959735853 8 26.105319412935035 36.13236921285987 19.2632299433045 18.4990814309006 9 26.510771851289434 16.42968704972404 19.330887245779405 37.72865385320712 10-11 25.312139639650365 26.62292845639905 27.063160496908495 21.001771407042096 12-13 25.948278161594068 23.642959077519883 27.493374246921952 22.915388513964096 14-15 22.669582869041964 25.256195937547787 25.112251718255212 26.961969475155033 16-17 22.998475598498516 28.58094395686413 25.513940567161214 22.906639877476138 18-19 22.843294260661466 27.31246927599965 27.429149026168275 22.415087437170612 20-21 23.43737718974408 26.595372211835038 27.605625751747624 22.361624846673255 22-23 23.254168423108343 26.443104388603494 27.34111645386929 22.961610734418876 24-25 22.97916348592706 26.649425370857365 27.45074547803033 22.920665665185247 26-27 22.87081683904729 26.94942630969534 27.21177865313252 22.96797819812485 28-29 22.75618507522688 26.99665483657855 27.403880732516008 22.84327935567856 30-31 22.909406234050667 26.726410108689464 27.229429630972152 23.13475402628772 32-33 22.669475579606242 26.90980674234377 27.211530674948907 23.20918700310108 34-35 23.209820321686557 26.64871447560937 27.30010463113654 22.84136057156753 36-37 23.144969956862784 26.589897302893938 27.099321823248175 23.1658109169951 38-39 22.896757786879583 26.71404708160809 27.411797599479105 22.977397532033223 40-41 23.190822463220915 26.69800295480933 27.054943159702155 23.0562314222676 42-43 22.88619519541478 26.72846764737476 26.978769479134492 23.406567678075973 44-45 23.096702488931637 26.79269129563504 26.982557160439395 23.12804905499393 46-47 23.23074556069836 27.112857029217775 26.493243458880343 23.163153951203526 48-49 23.196498465553624 27.034246156023052 26.480640105804504 23.28861527261882 50-51 23.13285580741773 27.154219435662014 26.501949996725195 23.21097476019506 52-53 23.43684879118974 27.00221628313172 26.416351625488637 23.144583300189904 54-55 23.194855364287438 26.835407338842472 26.615672245278198 23.354065051591892 56-57 23.087228437489507 27.160573804674815 26.515423423998463 23.236774333837218 58-59 23.221543130152682 26.816026170620116 26.50599310725386 23.45643759197334 60-61 23.304685816085083 26.845109300777498 26.67422449856921 23.17598038456821 62-63 23.170111473332277 26.91758786907154 26.682968604278607 23.22933205331758 64-65 23.070764824531402 26.8389116973668 26.619272248111354 23.471051229990444 66-67 22.988926224606374 27.11335204803042 26.507470279284345 23.39025144807886 68-69 22.99636835884407 26.850848311927628 26.833292759442244 23.319490569786062 70-71 22.92652267604587 26.781226600412687 26.789157452905503 23.503093270635937 72-73 23.08551230689348 26.898958189431365 26.603183071888264 23.412346431786894 74-75 23.043015098402662 27.060737746673492 26.528434328723975 23.367812826199874 76-77 23.294487513843738 26.828742572017195 26.616293332380646 23.26047658175842 78-79 23.1859648454292 26.949749754994478 26.48815102104487 23.376134378531443 80-81 23.045912104567964 27.14111921561641 26.59995970240752 23.213008977408105 82-83 23.19728573235189 27.206193887896426 26.40471233902434 23.191808040727352 84-85 23.22110294023085 27.090215059960627 26.33099638431007 23.357685615498454 86-87 23.120467783887882 26.9402297049247 26.6191772626278 23.320125248559613 88-89 23.14604718256148 27.023857106039046 26.471078636173296 23.359017075226177 90-91 23.15571051627981 27.235363631664487 26.52022258890014 23.088703263155562 92-93 23.08451514332361 27.084381769184144 26.576393015482118 23.254710072010127 94-95 23.00195953346619 26.897634028897045 26.68543461099235 23.414971826644415 96-97 23.16527730899478 26.876299805894067 26.5616190469158 23.39680383819535 98-99 22.929358506958504 26.942202847798896 26.375553360267425 23.75288528497518 100-101 22.855804309029317 26.834579032572687 26.729638765877695 23.579977892520304 102-103 23.008024440558792 26.706211737203024 26.747579497785384 23.5381843244528 104-105 22.974116784079488 26.720848039706013 26.732494057910955 23.57254111830354 106-107 22.883331217710452 26.89095603370485 26.726247052516587 23.499465696068107 108-109 22.890113224299 26.77520563611327 26.47736728617488 23.85731385341285 110-111 22.84262883449098 26.900315440133653 26.631054122572724 23.626001602802642 112-113 22.984137357159767 26.919266421238596 26.736529042454233 23.36006717914741 114-115 22.8611534103277 27.03205727565313 26.56798578301764 23.538803531001527 116-117 22.627283860000258 26.93455605986489 26.84115113355285 23.597008946582005 118-119 22.749124656280824 27.04348878116812 26.521092794769707 23.68629376778135 120-121 22.809264570588493 27.11622819290956 26.633246969876307 23.44126026662564 122-123 22.764271401088184 27.142466566418715 26.56817630810956 23.525085724383544 124-125 22.81666696045358 27.095244606191343 26.45449534474783 23.633593088607245 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 598.0 1 616.5 2 753.0 3 803.0 4 651.0 5 516.5 6 478.0 7 549.0 8 709.0 9 827.5 10 770.0 11 745.5 12 827.0 13 888.5 14 1010.5 15 1117.5 16 1193.0 17 1328.5 18 1500.0 19 1733.5 20 2058.5 21 2498.0 22 3221.0 23 4203.5 24 5386.5 25 7216.5 26 9646.5 27 12547.0 28 15266.0 29 18404.5 30 22303.0 31 26137.5 32 30269.5 33 33873.0 34 37486.0 35 40933.5 36 44933.0 37 48984.5 38 51471.5 39 53679.5 40 55652.5 41 58752.5 42 61754.0 43 64407.5 44 68439.5 45 72203.0 46 74114.0 47 75925.5 48 79483.5 49 80788.5 50 79386.5 51 78340.5 52 77612.0 53 77893.5 54 77451.0 55 73186.5 56 67970.5 57 63777.0 58 59782.5 59 54213.5 60 46332.5 61 37812.5 62 30081.5 63 23355.5 64 17531.0 65 13233.0 66 10169.0 67 8090.5 68 6200.0 69 4470.5 70 3302.5 71 2521.0 72 1848.0 73 1495.0 74 1200.0 75 850.0 76 618.0 77 391.0 78 265.0 79 194.0 80 137.0 81 99.0 82 64.5 83 44.5 84 33.5 85 25.5 86 21.5 87 16.5 88 16.5 89 12.0 90 8.0 91 9.0 92 7.5 93 6.0 94 5.5 95 4.5 96 3.5 97 2.0 98 4.5 99 4.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012908074977339423 2 0.004572602205994777 3 0.007621003676657961 4 0.007573372403678849 5 0.01600410772098172 6 3.810501838328981E-4 7 0.008287841498365532 8 0.0 9 0.0025720887408720618 10-11 0.0 12-13 0.0036676080193916443 14-15 0.018290408823979108 16-17 0.028793104515873358 18-19 0.005953909122389032 20-21 0.00885941677411488 22-23 0.002191038557039164 24-25 0.011312427332539161 26-27 0.01788554300365665 28-29 5.715752757493471E-4 30-31 0.0018814352826749343 32-33 0.009097573139010441 34-35 0.008645076045708876 36-37 0.010931377148706263 38-39 0.0 40-41 0.024291949219347252 42-43 0.009311913867416446 44-45 0.01655186736024151 46-47 0.0041915520221618785 48-49 0.023601295761150123 50-51 0.004691680388442558 52-53 0.0 54-55 0.016313710995345947 56-57 0.005025099299296343 58-59 3.5723454734334193E-4 60-61 0.0044535240235469955 62-63 0.02517312776946083 64-65 0.016147001539919055 66-67 0.0019766978286331585 68-69 0.019528821921436026 70-71 0.003096032743642297 72-73 0.011002824058174931 74-75 2.381563648955613E-5 76-77 0.006477853125159267 78-79 8.811785501135767E-4 80-81 0.0038105018383289804 82-83 0.0017385414637375975 84-85 0.0 86-87 0.005501412029087466 88-89 0.016099370266939944 90-91 0.020695788109424275 92-93 0.004929836753338118 94-95 0.02343458630572323 96-97 0.016647129906199733 98-99 7.144690946866839E-4 100-101 0.008168763315917751 102-103 0.0 104-105 0.00147656946235248 106-107 2.381563648955613E-5 108-109 0.0 110-111 6.430221852180154E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.381563648955613E-5 122-123 0.0 124-125 0.02067197247293472 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2099461.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.482305643512916 #Duplication Level Percentage of deduplicated Percentage of total 1 80.66873899366594 49.59700066685338 2 10.984519253707283 13.50707140206967 3 3.5590347405929075 6.5645298515104145 4 1.5488622975223785 3.8091050070393804 5 0.8329706949842262 2.560647943055478 6 0.5210888169515085 1.9222645146737523 7 0.3548095029508816 1.52701544139543 8 0.2296885707951025 1.1297426329956917 9 0.1864225302851934 1.0315518287248173 >10 1.010608804442901 11.484632467896567 >50 0.07250486274536547 3.045153238414605 >100 0.02948701285435254 3.1373519815363107 >500 0.0010269337827977763 0.4350711566190706 >1k 2.3698471910717865E-4 0.2488618672154626 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2264 0.10783720202471014 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.381563648955613E-5 0.0 12-13 0.0 0.0 0.0 4.763127297911226E-5 0.0 14-15 0.0 0.0 0.0 4.763127297911226E-5 0.0 16-17 0.0 0.0 0.0 7.144690946866839E-5 0.0 18-19 0.0 0.0 0.0 1.4289381893733678E-4 0.0 20-21 0.0 0.0 0.0 2.1434072840600515E-4 0.0 22-23 0.0 0.0 0.0 3.810501838328981E-4 0.0 24-25 0.0 0.0 0.0 9.28809823092689E-4 2.381563648955613E-5 26-27 0.0 0.0 0.0 0.0010478880055404696 4.763127297911226E-5 28-29 0.0 0.0 0.0 0.0015242007353315924 4.763127297911226E-5 30-31 0.0 0.0 0.0 0.0028102451057676232 4.763127297911226E-5 32-33 0.0 0.0 0.0 0.005739568393983027 4.763127297911226E-5 34-35 0.0 0.0 0.0 0.00997875168912402 4.763127297911226E-5 36-37 0.0 0.0 0.0 0.016504236087262397 4.763127297911226E-5 38-39 0.0 0.0 0.0 0.027959557238738893 4.763127297911226E-5 40-41 0.0 0.0 0.0 0.043868402413762386 4.763127297911226E-5 42-43 0.0 0.0 0.0 0.06006303522666056 4.763127297911226E-5 44-45 0.0 0.0 0.0 0.07868686296149345 4.763127297911226E-5 46-47 0.0 0.0 0.0 0.10100211435220754 4.763127297911226E-5 48-49 0.0 0.0 0.0 0.12748510212859396 4.763127297911226E-5 50-51 0.0 0.0 0.0 0.1565163630093629 4.763127297911226E-5 52-53 0.0 0.0 0.0 0.18692893080652606 4.763127297911226E-5 54-55 0.0 0.0 0.0 0.21888951497551037 4.763127297911226E-5 56-57 0.0 0.0 0.0 0.2570421646317793 4.763127297911226E-5 58-59 0.0 0.0 0.0 0.30250621469034195 4.763127297911226E-5 60-61 0.0 0.0 0.0 0.35494824624034454 4.763127297911226E-5 62-63 0.0 0.0 0.0 0.406056602146932 4.763127297911226E-5 64-65 0.0 0.0 0.0 0.4594036278835377 4.763127297911226E-5 66-67 0.0 0.0 0.0 0.521848226759154 4.763127297911226E-5 68-69 0.0 0.0 0.0 0.5930331642264371 4.763127297911226E-5 70-71 0.0 0.0 0.0 0.6767213108507374 4.763127297911226E-5 72-73 0.0 0.0 0.0 0.76457719386071 4.763127297911226E-5 74-75 0.0 0.0 0.0 0.8541716183344201 4.763127297911226E-5 76-77 0.0 0.0 0.0 0.9563645145111055 4.763127297911226E-5 78-79 0.0 0.0 0.0 1.073727971131638 4.763127297911226E-5 80-81 0.0 0.0 0.0 1.2121444504089383 4.763127297911226E-5 82-83 0.0 0.0 0.0 1.3551097162557437 4.763127297911226E-5 84-85 0.0 0.0 0.0 1.5021236403057736 4.763127297911226E-5 86-87 0.0 0.0 0.0 1.6555201549350047 4.763127297911226E-5 88-89 0.0 0.0 0.0 1.835137685339237 4.763127297911226E-5 90-91 0.0 0.0 0.0 2.038642299142494 4.763127297911226E-5 92-93 0.0 0.0 0.0 2.256603004294912 4.763127297911226E-5 94-95 0.0 0.0 0.0 2.4749209439946727 4.763127297911226E-5 96-97 0.0 0.0 0.0 2.704313154662078 4.763127297911226E-5 98-99 0.0 0.0 0.0 2.952543533792721 4.763127297911226E-5 100-101 0.0 0.0 0.0 3.246404672437354 4.763127297911226E-5 102-103 0.0 0.0 0.0 3.5629144813835554 4.763127297911226E-5 104-105 0.0 0.0 0.0 3.877114173590269 4.763127297911226E-5 106-107 2.381563648955613E-5 0.0 0.0 4.192742803986357 4.763127297911226E-5 108-109 4.763127297911226E-5 0.0 0.0 4.528852881763462 4.763127297911226E-5 110-111 4.763127297911226E-5 0.0 0.0 4.912213182335847 4.763127297911226E-5 112-113 4.763127297911226E-5 0.0 0.0 5.335893355485051 4.763127297911226E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2390 0.0 57.26151 1 GTATCAA 4285 0.0 52.76739 1 ATCAACG 5085 0.0 43.176517 3 TCAACGC 5205 0.0 42.18109 4 CAACGCA 5315 0.0 41.643948 5 AACGCAG 5330 0.0 41.40821 6 TATCAAC 5770 0.0 38.462265 2 GTACATG 7905 0.0 36.88307 1 ACGCAGA 6400 0.0 34.393143 7 TACATGG 8070 0.0 34.28319 2 CGCAGAG 6655 0.0 32.18367 8 ACATGGG 8375 0.0 32.111885 3 GCAGAGT 7565 0.0 27.291185 9 GAGTACA 6245 0.0 26.011362 1 CATGGGA 4725 0.0 25.184908 4 AGTACAT 6130 0.0 24.944483 2 CATGGGG 4685 0.0 22.98694 4 AGAGTAC 7270 0.0 22.305212 10-11 CAGAGTA 7665 0.0 21.85448 10-11 GAGTACT 3825 0.0 21.548826 12-13 >>END_MODULE