FastQCFastQC Report
Thu 26 May 2016
SRR1364526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099461
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47570.22658196556163704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40980.19519295666840203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27190.12950943123020622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36550.060.546781
GTATCAA60650.050.8468781
ATCAACG79950.038.1483423
TCAACGC81300.037.368344
TATCAAC83100.036.558922
CAACGCA83850.036.1178135
GTACATG83800.035.5902061
AACGCAG85200.035.545526
TACATGG86550.033.0368272
ACATGGG87850.031.7342533
ACGCAGA99100.030.2596287
CGCAGAG100900.029.7198148
GTGGTAT20950.026.4791151
GCAGAGT110950.026.2751039
CATGGGG46450.025.9053734
GAGTACT67700.025.792612-13
TGGTATC19750.025.637522
CATGGGA48350.025.1337834
GTACTTT75450.023.53752314-15
AGAGTAC107450.022.92290310-11
Produced by FastQC (version 0.11.5)