##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364526_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2099461 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0959784439911 31.0 30.0 34.0 26.0 34.0 2 30.00844359576101 31.0 30.0 34.0 26.0 34.0 3 30.261974382948765 31.0 30.0 34.0 26.0 34.0 4 33.84367225683163 35.0 35.0 37.0 30.0 37.0 5 33.256768284812146 35.0 33.0 37.0 27.0 37.0 6 33.29282896895918 35.0 33.0 37.0 27.0 37.0 7 33.081854342614605 35.0 33.0 37.0 26.0 37.0 8 33.01797985292416 35.0 33.0 37.0 26.0 37.0 9 34.27906924682097 38.0 34.0 39.0 25.0 39.0 10-11 34.20288755066181 38.0 34.0 39.0 25.0 39.0 12-13 34.07924557779354 38.0 34.0 39.0 25.0 39.0 14-15 35.04984993767448 39.0 34.0 41.0 25.0 41.0 16-17 35.048962090746144 39.0 34.0 41.0 25.0 41.0 18-19 35.04570149195436 39.0 34.0 41.0 25.0 41.0 20-21 35.05134960830422 39.0 34.0 41.0 25.0 41.0 22-23 34.9996811086274 39.0 34.0 41.0 25.0 41.0 24-25 34.95194409422228 39.0 34.0 41.0 24.5 41.0 26-27 34.82896110001567 39.0 34.0 41.0 24.0 41.0 28-29 34.67523569144652 39.0 34.0 41.0 24.0 41.0 30-31 34.52971596042984 38.5 33.5 41.0 23.5 41.0 32-33 34.33858880922294 38.0 33.0 40.5 22.5 41.0 34-35 34.16011704909022 38.0 33.0 40.0 21.5 41.0 36-37 33.961772807401516 38.0 33.0 40.0 19.5 41.0 38-39 33.78715370278371 38.0 33.0 40.0 18.0 41.0 40-41 33.8221257741868 38.0 33.0 40.0 18.0 41.0 42-43 33.69038386519207 38.0 33.0 40.0 16.5 41.0 44-45 33.538770189110444 38.0 33.0 40.0 15.0 41.0 46-47 33.32012264100166 38.0 32.0 40.0 13.0 41.0 48-49 33.06295782584196 38.0 32.0 40.0 9.0 41.0 50-51 32.89439956255439 38.0 32.0 40.0 8.0 41.0 52-53 32.70479208711188 38.0 31.5 40.0 7.0 41.0 54-55 32.50826140614186 37.5 31.0 40.0 4.0 41.0 56-57 32.28469640541073 37.0 31.0 40.0 2.0 41.0 58-59 32.07254481031084 37.0 31.0 40.0 2.0 41.0 60-61 31.821504900543523 36.0 31.0 40.0 2.0 41.0 62-63 31.64091997898508 36.0 30.5 39.5 2.0 41.0 64-65 31.49594562604402 36.0 31.0 39.0 2.0 41.0 66-67 31.195427064375096 35.0 30.0 39.0 2.0 41.0 68-69 30.906304761079152 35.0 30.0 39.0 2.0 40.5 70-71 30.589477013385817 35.0 30.0 38.0 2.0 40.0 72-73 30.30097653635862 35.0 30.0 37.0 2.0 40.0 74-75 29.947952355390264 35.0 29.0 37.0 2.0 39.0 76-77 29.63853912980522 35.0 29.0 36.0 2.0 39.0 78-79 29.362711429266845 34.0 29.0 36.0 2.0 38.5 80-81 29.092997917084432 34.0 29.0 35.5 2.0 37.0 82-83 28.820663017793613 34.0 29.0 35.0 2.0 37.0 84-85 28.610040148399996 34.0 29.0 35.0 2.0 36.5 86-87 28.402164650831807 34.0 29.0 35.0 2.0 36.0 88-89 28.233939806455087 34.0 28.5 35.0 2.0 36.0 90-91 28.10031050826855 34.0 28.0 35.0 2.0 35.5 92-93 27.987331510325745 34.0 28.0 35.0 2.0 35.0 94-95 27.879010374567567 34.0 28.0 35.0 2.0 35.0 96-97 27.76870349103889 34.0 27.0 35.0 2.0 35.0 98-99 27.6872866416666 34.0 27.0 35.0 2.0 35.0 100-101 27.59024054269167 34.0 27.0 35.0 2.0 35.0 102-103 26.313670985076648 31.5 24.5 34.0 2.0 35.0 104-105 27.254859223391144 33.5 26.5 35.0 2.0 35.0 106-107 27.417017034372158 34.0 27.0 35.0 2.0 35.0 108-109 27.42725228046627 34.0 27.0 35.0 2.0 35.0 110-111 27.348566846442967 34.0 27.0 35.0 2.0 35.0 112-113 27.266443625292396 34.0 27.0 35.0 2.0 35.0 114-115 27.16572944198535 34.0 27.0 35.0 2.0 35.0 116-117 27.044756249342093 34.0 26.5 35.0 2.0 35.0 118-119 26.90748863636905 34.0 25.5 35.0 2.0 35.0 120-121 26.70980003915291 34.0 25.0 35.0 2.0 35.0 122-123 26.41607703119991 34.0 25.0 35.0 2.0 35.0 124-125 24.61349198675279 31.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6232676762577967 1101 2 0.7684096960660263 1101 3 0.6450633967757753 1101 4 0.6449657460654876 1101 5 0.6476790794436909 1101 6 0.6227619075217703 1101 7 0.6902633196886399 1101 8 0.7638734688157882 1101 9 0.9091294362404341 1101 10-11 0.7893441070428011 1101 12-13 0.8414027262156338 1101 14-15 0.8233276180575686 1101 16-17 0.8904246851551463 1101 18-19 1.0451040070455804 1101 20-21 1.008829503095086 1101 22-23 0.9898872140766173 1101 24-25 1.0300202466687551 1101 26-27 1.0449935691406154 1101 28-29 1.1102092570462432 1101 30-31 1.0213147855234936 1101 32-33 1.1575133155800188 1101 34-35 1.2389255229820648 1101 36-37 1.16691836496436 1101 38-39 1.1464569792925872 1101 40-41 0.9508797667868691 1101 42-43 1.056057456090393 1101 44-45 0.959261304047196 1101 46-47 1.069042633498924 1101 48-49 1.0914458680735848 1101 50-51 1.022260893223617 1101 52-53 1.035390669257886 1101 54-55 0.9520515031352232 1101 56-57 1.0312981760331184 1101 58-59 1.0867928900190336 1101 60-61 0.9721833779507989 1101 62-63 0.9528567538049266 1101 64-65 0.9059135890030952 1101 66-67 1.0105846532527138 1101 68-69 1.091526142785515 1101 70-71 1.08463947113394 1101 72-73 1.0162334237228805 1101 74-75 1.0494624517314932 1101 76-77 1.0368191662992103 1101 78-79 1.022159007221564 1101 80-81 0.8192301597375895 1101 82-83 1.1149544553140558 1101 84-85 1.0666017635073963 1101 86-87 1.075282384914587 1101 88-89 1.0525967012587572 1101 90-91 1.030736348210624 1101 92-93 0.8614127166003058 1101 94-95 0.9792005894593601 1101 96-97 1.0367179976067327 1101 98-99 1.0232071158974883 1101 100-101 1.1138888409199446 1101 102-103 0.9541228168009113 1101 104-105 1.0559649762419596 1101 106-107 1.0131446034591853 1101 108-109 0.9361769431805129 1101 110-111 0.9772346868043265 1101 112-113 1.0456563086622452 1101 114-115 1.0264422319260973 1101 116-117 1.044222497286242 1101 118-119 0.9763563460338247 1101 120-121 1.0035210938031724 1101 122-123 0.9436592980566978 1101 124-125 1.2329428250128167 1102 1 0.38325766808873496 1102 2 -0.420753966011425 1102 3 0.12648453641580204 1102 4 0.2040534548159556 1102 5 0.39085141648966015 1102 6 0.34438908428327153 1102 7 0.410328171157758 1102 8 0.409322980006138 1102 9 0.7962607516059066 1102 10-11 0.788100927068264 1102 12-13 0.8171729876154004 1102 14-15 0.9970690459915161 1102 16-17 0.9847303954123419 1102 18-19 1.0836776168846214 1102 20-21 0.8687498104521865 1102 22-23 1.1312514309821893 1102 24-25 1.0348460212275157 1102 26-27 1.1026880466638858 1102 28-29 0.7261440381628574 1102 30-31 0.8972822775076921 1102 32-33 1.0629253236313616 1102 34-35 1.1710673770676863 1102 36-37 0.8187295502847363 1102 38-39 0.24516096824246603 1102 40-41 -0.20328646454277077 1102 42-43 0.1019485466814345 1102 44-45 -0.16165108496223013 1102 46-47 0.06910164585109158 1102 48-49 -0.32247904092316304 1102 50-51 -0.3437438473165315 1102 52-53 -0.4921718670329298 1102 54-55 -0.43809214077111136 1102 56-57 -0.46602625789127927 1102 58-59 -0.44949054157317647 1102 60-61 -0.5758213629966917 1102 62-63 -0.5820979579062389 1102 64-65 -0.5014607452367557 1102 66-67 -0.7031620068721836 1102 68-69 -0.44883912076191734 1102 70-71 -0.9562724331493513 1102 72-73 -0.7304165687720143 1102 74-75 -0.6005907357603775 1102 76-77 -0.7838692303550374 1102 78-79 -0.5847244297881744 1102 80-81 -0.8544860135573025 1102 82-83 -1.1249675689511704 1102 84-85 -0.8473832780069515 1102 86-87 -0.6061537140257549 1102 88-89 -0.777899456675609 1102 90-91 -0.6727318774819402 1102 92-93 -0.5622633853217174 1102 94-95 -0.37870795167993876 1102 96-97 -0.6050136844805394 1102 98-99 -0.8213611204060953 1102 100-101 -0.6656502782104639 1102 102-103 -0.5973904859783801 1102 104-105 -0.48886918733763096 1102 106-107 -0.45626490030955935 1102 108-109 -0.3377414403433612 1102 110-111 -0.28530347774146136 1102 112-113 -0.07837772685188682 1102 114-115 -0.2359197102289663 1102 116-117 -0.31950520149271355 1102 118-119 -0.031614802170373935 1102 120-121 0.16791031137575985 1102 122-123 0.11844757151311214 1102 124-125 0.27538283862588386 1103 1 0.3696097800663196 1103 2 -0.00524433079815978 1103 3 0.30340692986641926 1103 4 0.1901267179773285 1103 5 0.22942533053934255 1103 6 0.2585099511425142 1103 7 0.48465496667964203 1103 8 0.6119958580069422 1103 9 0.7380818751011162 1103 10-11 0.8223480869223039 1103 12-13 0.7479179034545069 1103 14-15 0.843359085953999 1103 16-17 0.7481003644589848 1103 18-19 0.8902264830991768 1103 20-21 0.928681126238402 1103 22-23 0.9385814148236307 1103 24-25 0.8359763455971887 1103 26-27 0.8945621194063307 1103 28-29 0.9018994818620527 1103 30-31 0.9635484928917961 1103 32-33 0.9388197268184371 1103 34-35 0.8292878381032338 1103 36-37 1.0233390792895563 1103 38-39 0.9392508507519537 1103 40-41 0.8200237570898636 1103 42-43 0.9585248431889468 1103 44-45 0.7244770387850608 1103 46-47 0.9009312205627396 1103 48-49 0.8114839493610404 1103 50-51 0.9187713917351061 1103 52-53 0.866984220115846 1103 54-55 0.9615773526104974 1103 56-57 0.8798973216224795 1103 58-59 0.9392442865303252 1103 60-61 1.06285839788967 1103 62-63 0.9881525204450092 1103 64-65 0.8564253865030373 1103 66-67 0.770698008749136 1103 68-69 0.858933673759875 1103 70-71 0.8133785047497071 1103 72-73 0.7429728442713746 1103 74-75 0.8275756808825285 1103 76-77 0.9801197024774204 1103 78-79 0.8094827159322868 1103 80-81 0.8146929786609682 1103 82-83 0.8415986232902419 1103 84-85 0.886773790640067 1103 86-87 0.9248110574194648 1103 88-89 1.0793764931047392 1103 90-91 0.9838737606572252 1103 92-93 0.8817320150675805 1103 94-95 0.9445020021586501 1103 96-97 0.9966316681769456 1103 98-99 0.997534425451569 1103 100-101 0.9064366350085606 1103 102-103 0.8115050392161614 1103 104-105 0.8186441300437721 1103 106-107 0.9991832414559774 1103 108-109 1.07808175772918 1103 110-111 0.9976790474572148 1103 112-113 0.9214592927822132 1103 114-115 0.9981774870253197 1103 116-117 1.0002937876674132 1103 118-119 0.7708479835489257 1103 120-121 0.954526033117908 1103 122-123 1.0686223815157163 1103 124-125 1.377068949289292 1104 1 0.3747759678429752 1104 2 0.4317887514414913 1104 3 0.20501444714528816 1104 4 -0.07512139882005897 1104 5 0.28162693029083385 1104 6 0.3997287909343967 1104 7 0.45890168563592226 1104 8 0.4165834948788856 1104 9 0.4279048152269169 1104 10-11 0.4114313361263129 1104 12-13 0.41569087076055666 1104 14-15 0.48264230409486686 1104 16-17 0.531803866827552 1104 18-19 0.43568349948498764 1104 20-21 0.3130874320549708 1104 22-23 0.31191856633407156 1104 24-25 0.5272505165997501 1104 26-27 0.3929905188782641 1104 28-29 0.5476457737002178 1104 30-31 0.48502670986780316 1104 32-33 0.4842262656085268 1104 34-35 0.5409899038017727 1104 36-37 0.5830851381542033 1104 38-39 0.6592914269731267 1104 40-41 0.5206401661913631 1104 42-43 0.610144979866746 1104 44-45 0.5784570030624607 1104 46-47 0.5637447640899467 1104 48-49 0.4555809828315702 1104 50-51 0.397817365176131 1104 52-53 0.43936423875373265 1104 54-55 0.08775775177178957 1104 56-57 0.17180925763241817 1104 58-59 0.3397849792382459 1104 60-61 0.4851549343500352 1104 62-63 0.4712425258810562 1104 64-65 0.4764368410343742 1104 66-67 0.6527842866088847 1104 68-69 0.30847452178348433 1104 70-71 -0.1150539717587975 1104 72-73 -0.1361993231589267 1104 74-75 0.21337788636517274 1104 76-77 0.34172178370751993 1104 78-79 0.40120730240429126 1104 80-81 0.36624419400615693 1104 82-83 0.3649707208284845 1104 84-85 0.39684212953887865 1104 86-87 0.41068800857268073 1104 88-89 0.4128013979738867 1104 90-91 0.43418147984647604 1104 92-93 0.4218869424574976 1104 94-95 0.5069544367319203 1104 96-97 0.5613934527540998 1104 98-99 0.49102378632754906 1104 100-101 0.4861524073157746 1104 102-103 0.4093995410411182 1104 104-105 0.471478641187673 1104 106-107 0.4736765240528591 1104 108-109 0.41480032571862324 1104 110-111 0.40911901794723704 1104 112-113 0.4762109818402216 1104 114-115 0.4184607439951087 1104 116-117 0.4490570865724379 1104 118-119 0.4923135811260053 1104 120-121 0.49408202442022997 1104 122-123 0.6026636954863598 1104 124-125 0.9957883715149656 1105 1 0.10297173950352345 1105 2 -0.11041938138401264 1105 3 0.17394086552435084 1105 4 0.17582621447161273 1105 5 -0.029607969854367866 1105 6 0.34314871323998375 1105 7 0.32768784356386504 1105 8 0.530673018297442 1105 9 0.610002412173877 1105 10-11 0.5189960878006872 1105 12-13 0.5215793389947976 1105 14-15 0.4920713034182711 1105 16-17 0.4709051736444323 1105 18-19 0.501511834376771 1105 20-21 0.47364763761825657 1105 22-23 0.5521668539402214 1105 24-25 0.5844821021890354 1105 26-27 0.5798583015467784 1105 28-29 0.6276833665419872 1105 30-31 0.6289861977841227 1105 32-33 0.7740047377198849 1105 34-35 0.8456884388592769 1105 36-37 0.9273679093054028 1105 38-39 1.060045754521191 1105 40-41 0.7844495488173067 1105 42-43 0.7102818193312501 1105 44-45 0.7377430811673875 1105 46-47 0.6732066428957992 1105 48-49 0.6913458774271746 1105 50-51 0.579003459245655 1105 52-53 0.6319817496438773 1105 54-55 0.5260558869539764 1105 56-57 0.5853590253854364 1105 58-59 0.40471473497980526 1105 60-61 0.31945906766208054 1105 62-63 0.5845322986515207 1105 64-65 0.3363080979048547 1105 66-67 0.6106326000624875 1105 68-69 0.6414684172229279 1105 70-71 0.7197821249714984 1105 72-73 0.636836270306226 1105 74-75 0.8047461669773028 1105 76-77 0.7698531267868631 1105 78-79 0.6749272832467668 1105 80-81 0.6803059694221893 1105 82-83 0.6296876151808171 1105 84-85 0.5680809448132038 1105 86-87 0.7526958236265422 1105 88-89 0.7278449965477165 1105 90-91 0.5518484049876875 1105 92-93 0.6032733542986719 1105 94-95 0.515339845083691 1105 96-97 0.5140616322570679 1105 98-99 0.5039245168982447 1105 100-101 0.576701171579387 1105 102-103 0.1809141258473801 1105 104-105 0.4037071858999681 1105 106-107 0.38813513391594157 1105 108-109 0.4867923282121396 1105 110-111 0.332951701293112 1105 112-113 0.330050849257983 1105 114-115 0.3518218253309655 1105 116-117 0.08108018203316902 1105 118-119 0.19617169380570232 1105 120-121 0.3602781153728607 1105 122-123 0.2625575688794477 1105 124-125 0.762253960190737 1106 1 -0.14673440317659825 1106 2 -0.6093944212140734 1106 3 -0.030695943350377064 1106 4 -0.1272793499350655 1106 5 0.2350070062475993 1106 6 0.39065682677040314 1106 7 0.4003360998924208 1106 8 0.4799655184449563 1106 9 0.5126589295515842 1106 10-11 0.42677947129038785 1106 12-13 0.585253145568231 1106 14-15 0.48819834655274974 1106 16-17 0.4416758115154451 1106 18-19 0.6198236299748103 1106 20-21 0.5585806641126254 1106 22-23 0.5604043000958825 1106 24-25 0.6273079710743801 1106 26-27 0.6726930215562206 1106 28-29 0.6819587545162662 1106 30-31 0.5763574139285694 1106 32-33 0.5220887643912704 1106 34-35 0.58228311638333 1106 36-37 0.6115367887530923 1106 38-39 0.5874149054033566 1106 40-41 0.6558329795130078 1106 42-43 0.4440397115835637 1106 44-45 0.45446196946620177 1106 46-47 0.3636527395500764 1106 48-49 0.638015494468533 1106 50-51 0.6890828243250269 1106 52-53 0.48823949437745995 1106 54-55 0.5382329684433245 1106 56-57 0.0884486577687511 1106 58-59 0.5307597576976946 1106 60-61 0.5498842166560038 1106 62-63 0.4154581262316235 1106 64-65 0.5366559470817016 1106 66-67 0.5067106669769856 1106 68-69 0.5520605574247348 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2216 84-85 -1.4302192743661806 2216 86-87 -1.5492454012595296 2216 88-89 -1.5523559969681493 2216 90-91 -1.452088702756953 2216 92-93 -1.502207042742679 2216 94-95 -1.4972584982048787 2216 96-97 -1.4930914447404113 2216 98-99 -1.5212588017440467 2216 100-101 -1.6006097474227872 2216 102-103 -1.5508970878881065 2216 104-105 -1.5726768744117727 2216 106-107 -1.623550767977104 2216 108-109 -1.6319842365662254 2216 110-111 -1.5874044241410026 2216 112-113 -1.4735251873728963 2216 114-115 -1.5236516641836424 2216 116-117 -1.5954203116223091 2216 118-119 -1.6076430494295764 2216 120-121 -1.7125907711922252 2216 122-123 -1.7303494870176799 2216 124-125 -1.994135912663321 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62905.0 3 21793.0 4 10907.0 5 8626.0 6 9098.0 7 10751.0 8 11940.0 9 13002.0 10 14271.0 11 15611.0 12 16653.0 13 18493.0 14 20375.0 15 18311.0 16 13318.0 17 11782.0 18 10949.0 19 10200.0 20 10354.0 21 10705.0 22 12268.0 23 15354.0 24 19834.0 25 25909.0 26 32462.0 27 37561.0 28 42954.0 29 50817.0 30 59280.0 31 69546.0 32 84030.0 33 105193.0 34 135475.0 35 186910.0 36 301221.0 37 445731.0 38 154296.0 39 576.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64703638721489 19.072345335265847 13.671189640704858 24.6094286368144 2 15.716224307095963 20.106160581215846 39.15209665718963 25.025518454498556 3 19.521439074124263 24.25860732826187 28.707225330692022 27.512728266921844 4 13.08844086175299 16.54347191789051 36.1715801538218 34.1965070665347 5 13.603110512650629 38.10349418255448 33.90632167018106 14.387073634613836 6 32.88629795933337 36.55409650381693 16.63931837743116 13.920287159418537 7 28.908890246940455 31.954455840751677 20.497824868189348 18.638829044118523 8 26.31648894363641 36.00003620195738 18.92138173344499 18.762093120961218 9 26.3291267065704 15.855826417819305 19.326603111576432 38.48844376403386 10-11 25.500107170925716 26.479887589606804 26.811283907690107 21.20872133177737 12-13 26.236376860537064 23.443898219590647 27.623232820233383 22.696492099638906 14-15 22.858330237299114 24.96632467396378 25.40829546645328 26.76704962228382 16-17 22.83946618603468 28.303519289264184 25.935289012688035 22.9217255120131 18-19 22.74217725536969 26.590949036949674 28.340405911529025 22.326467796151608 20-21 23.222425663297823 25.899766317969508 28.5507665399341 22.32704147879857 22-23 23.015216762778635 25.73038984767995 28.445300960579882 22.809092428961527 24-25 22.872561100206195 25.97197566423001 28.386762126088556 22.76870110947524 26-27 22.684870069032005 26.332187166134545 28.254061399568748 22.7288813652647 28-29 22.59968153730886 26.341832498912815 28.456851544277317 22.601634419501003 30-31 22.722760746686888 26.017225373560166 28.418937050985942 22.841076828767 32-33 22.498584160410694 26.172812926746435 28.385976210084397 22.94262670275847 34-35 22.947437461329358 26.18343470062078 28.166372226014204 22.70275561203566 36-37 22.990639025921414 26.006413074593908 28.036696085328565 22.96625181415611 38-39 22.790802020137548 26.19300858648958 28.192069297786432 22.82412009558644 40-41 22.948151930424046 26.06407073053512 28.09654477982682 22.891232559214007 42-43 22.773226080408257 26.101104045276387 27.928501648756516 23.197168225558844 44-45 22.977183191304814 26.228779672496895 27.81854485508423 22.975492281114057 46-47 23.102262914147964 26.56212713644121 27.332777317606755 23.00283263180407 48-49 23.18049728001616 26.582179902365414 27.171426380390013 23.06589643722841 50-51 23.123059471707137 26.716696980009864 27.080885779942037 23.079357768340962 52-53 23.443421906860856 26.54483698434979 26.97451869789436 23.037222410894987 54-55 23.118743334598737 26.45764793916153 27.114125958996144 23.30948276724359 56-57 23.067146952373786 26.754603664258568 27.035315480449253 23.14293390291839 58-59 23.15032883651532 26.509450700720073 26.981360572245794 23.358859890518815 60-61 23.222627140966182 26.41701846331034 27.222177501749258 23.138176893974215 62-63 23.104835002888837 26.485083552397498 27.288623127555123 23.121458317158545 64-65 22.91627916613776 26.35852227680505 27.242130653151442 23.483067903905745 66-67 22.86948888309904 26.763631236779347 27.093858852343526 23.273021027778082 68-69 22.940769083112283 26.44104844052831 27.33030049141184 23.287881984947564 70-71 22.812283724251127 26.402895790872037 27.345709208220587 23.439111276656245 72-73 23.016979119878865 26.50747025069768 27.01807749703376 23.457473132389694 74-75 22.983876153464 26.64707252468162 26.927457218292332 23.44159410356205 76-77 23.190285506613364 26.492394952799792 27.013314369735852 23.304005170850996 78-79 23.079137931116605 26.62871565606601 26.879922989757848 23.41222342305954 80-81 22.951931199434902 26.7254500503582 26.98075385593292 23.34186489427398 82-83 23.043795526566104 26.844461507024896 26.78804226418114 23.323700702227857 84-85 23.101858048327642 26.684134642177206 26.751937759262972 23.46206955023218 86-87 23.064681839767445 26.609282096690535 26.92164798488755 23.404388078654474 88-89 23.02300757488072 26.661431934912137 26.817449804447758 23.498110685759386 90-91 22.951610663787196 26.913080765272795 26.857447425183757 23.277861145756255 92-93 22.93635664741371 26.663008509977526 26.94230019938859 23.45833464322017 94-95 22.86908401727872 26.54836169855025 27.033533845115482 23.54902043905555 96-97 22.994409772644396 26.495476919918342 26.917381770061617 23.592731537375638 98-99 22.74422037559193 26.484611286334843 26.784557056025367 23.98661128204786 100-101 22.676237911207107 26.42137911373819 27.060891053620452 23.841491921434248 102-103 22.8337892440012 26.336688321431073 26.97075582732901 23.858766607238717 104-105 22.80047116855231 26.266789428334224 27.015171989382036 23.91756741373143 106-107 22.73006027578392 26.433368811157866 27.040318257282266 23.79625265577595 108-109 22.71286426033361 26.31667190610919 26.798748249549888 24.171715584007316 110-111 22.599822144657267 26.393513757591837 26.979998180483637 24.02666591726726 112-113 22.715782765195446 26.434689665585598 27.006979410429626 23.842548158789327 114-115 22.610684361367035 26.50587460305288 26.84100823973391 24.04243279584617 116-117 22.409632758122203 26.36691036413632 27.071495969679837 24.15196090806164 118-119 22.381649385246977 26.485154999306964 26.876398275557396 24.256797339888667 120-121 22.438642058869902 26.532370728807592 26.963985209410307 24.065002002912202 122-123 22.402570879356297 26.554884954196744 26.876887927008212 24.165656239438743 124-125 22.363031215692555 26.442098448868528 26.774262439107638 24.420607896331276 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196.0 1 161.0 2 266.5 3 428.5 4 304.0 5 209.5 6 427.5 7 1036.0 8 1723.5 9 1932.0 10 1512.5 11 995.0 12 896.5 13 951.0 14 1072.0 15 1249.5 16 1435.0 17 1659.0 18 1885.5 19 2132.0 20 2492.5 21 2956.0 22 3609.5 23 4633.0 24 5898.0 25 7618.5 26 10025.5 27 12953.0 28 15617.5 29 18839.5 30 22978.0 31 26585.5 32 30367.0 33 33954.0 34 37416.5 35 40515.0 36 44336.0 37 48777.0 38 51318.0 39 53076.0 40 55191.5 41 57996.5 42 60840.5 43 64057.0 44 68126.5 45 71742.0 46 73582.5 47 75239.0 48 79261.5 49 81329.0 50 79879.5 51 78363.0 52 77855.0 53 78374.5 54 78111.5 55 74014.0 56 68275.0 57 63733.5 58 60084.0 59 54668.0 60 46414.5 61 37554.5 62 29524.5 63 22855.0 64 17010.0 65 12664.0 66 9646.5 67 7565.5 68 5684.0 69 3991.0 70 2890.5 71 2231.5 72 1708.0 73 1408.0 74 1111.5 75 769.5 76 533.0 77 317.0 78 195.5 79 123.5 80 78.0 81 39.5 82 24.0 83 17.0 84 12.5 85 9.5 86 5.0 87 4.5 88 4.0 89 2.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09626280269078587 2 0.0 3 0.0 4 0.09378597649587203 5 0.0 6 0.0 7 0.0022863011029973885 8 0.006049171668347257 9 0.0035723454734334194 10-11 5.239440027702348E-4 12-13 0.0 14-15 4.763127297911226E-5 16-17 1.4289381893733678E-4 18-19 0.0018099883732062658 20-21 0.0012860443704360309 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.381563648955613E-5 52-53 0.0 54-55 0.0 56-57 0.0073828473117624 58-59 6.430221852180154E-4 60-61 0.0 62-63 0.0 64-65 7.144690946866839E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0011431505514986943 76-77 0.0 78-79 0.0 80-81 7.144690946866839E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0010478880055404696 90-91 2.381563648955613E-5 92-93 0.002048144738101827 94-95 0.0 96-97 0.008216394588896865 98-99 0.003881948747797649 100-101 0.002262485466507832 102-103 0.0 104-105 0.0 106-107 0.001309860006925587 108-109 4.763127297911226E-5 110-111 9.526254595822452E-5 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.573629136240206E-4 122-123 0.004834574207379894 124-125 0.08106842661044907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2099461.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.69374115652162 #Duplication Level Percentage of deduplicated Percentage of total 1 78.75059648265962 45.434165293922455 2 11.96384093105605 13.804774838282926 3 3.867849370712683 6.694521012789377 4 1.778072372748969 4.103345889237649 5 0.9185071692726378 2.6496057437212475 6 0.5695846596319019 1.9716881951717047 7 0.37358255241659943 1.508736255580117 8 0.26716698701889 1.233109039570849 9 0.20531938601233987 1.0661079159910694 >10 1.1703376721951402 12.644312597360061 >50 0.09293218281956335 3.6710983747657515 >100 0.04054956124795362 4.040258636733439 >500 0.0012455041557187703 0.49327980574787966 >1k 4.1516805190625083E-4 0.6849964011254595 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4757 0.22658196556163704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4098 0.19519295666840203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2719 0.12950943123020622 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.763127297911226E-5 0.0 12-13 0.0 0.0 0.0 9.526254595822452E-5 0.0 14-15 0.0 0.0 0.0 9.526254595822452E-5 0.0 16-17 0.0 0.0 0.0 1.1907818244778065E-4 0.0 18-19 0.0 0.0 0.0 1.9052509191644904E-4 0.0 20-21 0.0 0.0 0.0 2.619720013851174E-4 0.0 22-23 0.0 0.0 0.0 4.524970933015665E-4 0.0 24-25 0.0 0.0 0.0 0.0010240723690509135 0.0 26-27 0.0 0.0 0.0 0.0011431505514986943 0.0 28-29 0.0 0.0 0.0 0.0015718320083107046 0.0 30-31 0.0 0.0 0.0 0.0028340607422571796 0.0 32-33 0.0 0.0 0.0 0.005739568393983027 0.0 34-35 0.0 0.0 0.0 0.009883489143165794 0.0 36-37 2.381563648955613E-5 0.0 0.0 0.016456604814283286 0.0 38-39 4.763127297911226E-5 0.0 0.0 0.027959557238738893 0.0 40-41 4.763127297911226E-5 0.0 0.0 0.043796955504293716 0.0 42-43 4.763127297911226E-5 0.0 0.0 0.05975343195229633 0.0 44-45 4.763127297911226E-5 0.0 0.0 0.07802002513978587 0.0 46-47 4.763127297911226E-5 0.0 0.0 0.09993041071017753 0.0 48-49 4.763127297911226E-5 0.0 0.0 0.12565129811889814 0.0 50-51 4.763127297911226E-5 0.0 0.0 0.1538966429955117 0.0 52-53 4.763127297911226E-5 0.0 0.0 0.18352329478851953 0.0 54-55 4.763127297911226E-5 0.0 0.0 0.21445980658845293 0.0 56-57 4.763127297911226E-5 0.0 0.0 0.2514454900567336 0.0 58-59 4.763127297911226E-5 0.0 0.0 0.29529007683400643 0.0 60-61 4.763127297911226E-5 0.0 0.0 0.3467556672879372 0.0 62-63 4.763127297911226E-5 0.0 0.0 0.3970066602809007 0.0 64-65 4.763127297911226E-5 0.0 0.0 0.4491152729200495 0.0 66-67 4.763127297911226E-5 0.0 0.0 0.509773699058949 0.0 68-69 4.763127297911226E-5 0.0 0.0 0.5795535139733483 0.0 70-71 4.763127297911226E-5 0.0 0.0 0.6607648344027348 0.0 72-73 4.763127297911226E-5 0.0 0.0 0.7460486286718353 0.0 74-75 4.763127297911226E-5 0.0 0.0 0.8326660985843509 0.0 76-77 4.763127297911226E-5 0.0 0.0 0.9330728220243196 0.0 78-79 4.763127297911226E-5 0.0 0.0 1.0466257768065232 0.0 80-81 4.763127297911226E-5 0.0 0.0 1.1826845080713575 0.0 82-83 4.763127297911226E-5 0.0 0.0 1.3226490037204788 0.0 84-85 4.763127297911226E-5 0.0 0.0 1.4665668950268664 0.0 86-87 4.763127297911226E-5 0.0 0.0 1.617034086367882 0.0 88-89 4.763127297911226E-5 0.0 0.0 1.7926982211148481 0.0 90-91 4.763127297911226E-5 0.0 0.0 1.9921541767148807 0.0 92-93 4.763127297911226E-5 0.0 0.0 2.208304893494092 0.0 94-95 4.763127297911226E-5 0.0 0.0 2.4233362753582943 0.0 96-97 4.763127297911226E-5 0.0 0.0 2.6488941685508802 0.0 98-99 4.763127297911226E-5 0.0 0.0 2.8925281298390395 0.0 100-101 4.763127297911226E-5 0.0 0.0 3.182578766645344 0.0 102-103 4.763127297911226E-5 0.0 0.0 3.4948493922964037 0.0 104-105 4.763127297911226E-5 0.0 0.0 3.8054529233931946 0.0 106-107 4.763127297911226E-5 0.0 0.0 4.118056967955109 0.0 108-109 4.763127297911226E-5 0.0 0.0 4.451594956991341 0.0 110-111 4.763127297911226E-5 0.0 0.0 4.832025934275512 0.0 112-113 4.763127297911226E-5 0.0 0.0 5.252633890317563 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3655 0.0 60.54678 1 GTATCAA 6065 0.0 50.846878 1 ATCAACG 7995 0.0 38.148342 3 TCAACGC 8130 0.0 37.36834 4 TATCAAC 8310 0.0 36.55892 2 CAACGCA 8385 0.0 36.117813 5 GTACATG 8380 0.0 35.590206 1 AACGCAG 8520 0.0 35.54552 6 TACATGG 8655 0.0 33.036827 2 ACATGGG 8785 0.0 31.734253 3 ACGCAGA 9910 0.0 30.259628 7 CGCAGAG 10090 0.0 29.719814 8 GTGGTAT 2095 0.0 26.479115 1 GCAGAGT 11095 0.0 26.275103 9 CATGGGG 4645 0.0 25.905373 4 GAGTACT 6770 0.0 25.7926 12-13 TGGTATC 1975 0.0 25.63752 2 CATGGGA 4835 0.0 25.133783 4 GTACTTT 7545 0.0 23.537523 14-15 AGAGTAC 10745 0.0 22.922903 10-11 >>END_MODULE