FastQCFastQC Report
Thu 26 May 2016
SRR1364525_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364525_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431735
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64890.45322633029156933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38840.2712792520962329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27290.19060789880808948No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20950.14632596115901336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17120.11957520071800996No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16850.11768937687491052No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70950.042.7653731
GTACATG85600.036.5583421
ATCAACG82800.035.8545883
TCAACGC82750.035.588674
AACGCAG83000.035.406086
CAACGCA83600.035.3691525
GGTATCA45000.035.299881
TATCAAC87100.034.357722
TACATGG90000.033.7794762
ACATGGG92150.031.7001023
ACGCAGA93400.031.3999567
CGCAGAG92400.030.7794468
ATGGGAG15450.029.6508225
GAGTACT37250.029.08048812-13
CATGGGG42000.028.4722184
GCAGAGT97200.027.7291669
CATGGGA53500.026.9113884
GTACTTT41150.026.25205814-15
GAACAAA23000.023.7976721
AGTACTT42200.023.624312-13
Produced by FastQC (version 0.11.5)