##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364525_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1431735 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.694558699759384 31.0 30.0 33.0 20.0 34.0 2 28.69621543092821 31.0 28.0 34.0 16.0 34.0 3 28.91436404083158 31.0 28.0 34.0 22.0 34.0 4 32.15428274087034 35.0 32.0 37.0 20.0 37.0 5 32.42358606865097 35.0 33.0 37.0 25.0 37.0 6 32.416888600194866 35.0 33.0 37.0 23.0 37.0 7 32.325340234051694 35.0 33.0 37.0 22.0 37.0 8 32.19798216848788 35.0 33.0 37.0 19.0 37.0 9 33.314747491679675 38.0 33.0 39.0 17.0 39.0 10-11 33.31406754741624 38.0 33.0 39.0 17.0 39.0 12-13 33.24816324249949 38.0 33.0 39.0 17.0 39.0 14-15 34.023680709069765 39.0 33.0 41.0 16.5 41.0 16-17 34.17247849636979 39.0 33.0 41.0 17.0 41.0 18-19 33.95897564842656 39.0 33.0 41.0 17.5 41.0 20-21 33.66370766936619 39.0 32.0 41.0 14.5 41.0 22-23 33.57833432862925 38.5 32.0 41.0 14.5 41.0 24-25 33.38732412073463 38.0 32.0 41.0 13.0 41.0 26-27 33.29156687515497 38.0 32.0 41.0 12.5 41.0 28-29 33.33734280435975 38.0 32.0 40.5 12.5 41.0 30-31 33.2538933531694 38.0 32.0 40.0 12.5 41.0 32-33 33.216983939066935 38.0 32.0 40.0 10.0 41.0 34-35 32.7125072027994 38.0 31.0 40.0 9.0 41.0 36-37 32.5554030599238 38.0 30.5 40.0 8.0 41.0 38-39 32.32999193286467 38.0 30.0 40.0 8.0 41.0 40-41 32.52197822921141 38.0 31.0 40.0 7.5 41.0 42-43 32.36755195619301 38.0 31.0 40.0 2.0 41.0 44-45 32.17794529015495 38.0 30.5 40.0 2.0 41.0 46-47 32.082001906777435 37.5 30.0 40.0 2.0 41.0 48-49 31.88980642367477 37.0 30.0 40.0 2.0 41.0 50-51 31.706554983987957 37.0 30.0 40.0 2.0 41.0 52-53 31.617092548551234 37.0 30.0 40.0 2.0 41.0 54-55 31.61281976064006 37.0 30.0 40.0 2.0 41.0 56-57 31.459969198210562 36.5 30.0 40.0 2.0 41.0 58-59 31.241386150370005 36.0 30.0 40.0 2.0 41.0 60-61 31.00784397950738 36.0 30.0 40.0 2.0 41.0 62-63 30.711213667333688 35.0 29.0 39.0 2.0 41.0 64-65 30.41488997614782 35.0 29.0 39.0 2.0 41.0 66-67 30.151038774633573 35.0 29.0 39.0 2.0 40.5 68-69 30.106497012366116 35.0 29.0 38.5 2.0 40.0 70-71 29.863580201643458 35.0 29.0 37.5 2.0 40.0 72-73 29.58353955166284 35.0 29.0 37.0 2.0 39.5 74-75 29.304931429349704 35.0 29.0 36.5 2.0 39.0 76-77 29.011289100287414 34.5 29.0 36.0 2.0 39.0 78-79 28.72213049202541 34.0 29.0 36.0 2.0 38.0 80-81 28.454527548743307 34.0 28.0 35.0 2.0 37.0 82-83 28.23262929243191 34.0 28.0 35.0 2.0 37.0 84-85 28.01626872291311 34.0 28.0 35.0 2.0 36.0 86-87 27.83007365189787 34.0 27.5 35.0 2.0 36.0 88-89 27.642130003108115 34.0 27.0 35.0 2.0 36.0 90-91 27.519741083370874 34.0 27.0 35.0 2.0 35.5 92-93 27.40517169727638 34.0 27.0 35.0 2.0 35.0 94-95 27.29990640726112 34.0 27.0 35.0 2.0 35.0 96-97 27.18719735146518 34.0 26.5 35.0 2.0 35.0 98-99 27.088877830045362 34.0 26.0 35.0 2.0 35.0 100-101 26.995066824517107 34.0 26.0 35.0 2.0 35.0 102-103 25.817853862621227 32.0 24.5 34.0 2.0 35.0 104-105 26.68397678341313 33.5 25.0 35.0 2.0 35.0 106-107 26.831478939887617 34.0 25.0 35.0 2.0 35.0 108-109 26.775768211296086 34.0 25.0 35.0 2.0 35.0 110-111 26.688323258144838 34.0 25.0 35.0 2.0 35.0 112-113 26.59322884472336 34.0 25.0 35.0 2.0 35.0 114-115 26.46322189511327 34.0 25.0 35.0 2.0 35.0 116-117 26.348616538675103 34.0 25.0 35.0 2.0 35.0 118-119 26.194788840113567 34.0 24.5 35.0 2.0 35.0 120-121 26.0121859142928 34.0 24.0 35.0 2.0 35.0 122-123 25.773373215015347 34.0 23.0 35.0 2.0 35.0 124-125 24.33634680998928 32.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2786350521556251 1101 2 1.3165503950180408 1101 3 1.2395045215308471 1101 4 1.443664333755322 1101 5 1.192493152504369 1101 6 1.1679507944268792 1101 7 1.3133694106275726 1101 8 1.2828164457826219 1101 9 1.5651627498610239 1101 10-11 1.5759629317712651 1101 12-13 1.4241750589310698 1101 14-15 1.8361027138040953 1101 16-17 1.614728623845501 1101 18-19 1.8387632207869729 1101 20-21 2.27516230911683 1101 22-23 2.0789738140222624 1101 24-25 2.2742729617264814 1101 26-27 2.3983348334644177 1101 28-29 2.1606235609070055 1101 30-31 2.2128361791557722 1101 32-33 2.1967874339761337 1101 34-35 2.5732424848264728 1101 36-37 2.5218291531870634 1101 38-39 2.3089961554588427 1101 40-41 2.105410073955966 1101 42-43 2.0087028561546134 1101 44-45 1.9110283340602336 1101 46-47 1.767271125646559 1101 48-49 1.752726555101276 1101 50-51 1.595136720211869 1101 52-53 1.2779177180202126 1101 54-55 1.2713987214905629 1101 56-57 1.3793829073982202 1101 58-59 1.3913504722272876 1101 60-61 1.3290953864513533 1101 62-63 1.287357194338746 1101 64-65 1.4431090088323089 1101 66-67 1.4077462540712418 1101 68-69 1.2743039350413277 1101 70-71 1.1731746587074596 1101 72-73 0.9584867801104515 1101 74-75 1.0559923379239429 1101 76-77 1.2517411459797785 1101 78-79 1.2437915668573076 1101 80-81 1.2563295308253402 1101 82-83 1.1247890359191928 1101 84-85 1.168466679640261 1101 86-87 1.2071374831815689 1101 88-89 1.2486319628193243 1101 90-91 1.3074570311550353 1101 92-93 1.1041724603688152 1101 94-95 1.1765739346944706 1101 96-97 1.1775347066481139 1101 98-99 1.1658753097631553 1101 100-101 1.2816411897275763 1101 102-103 1.1765946066372202 1101 104-105 1.2001771914944719 1101 106-107 1.1954725533711255 1101 108-109 1.268712498324927 1101 110-111 1.0336632988321846 1101 112-113 1.1210178382126053 1101 114-115 1.2621638520982117 1101 116-117 1.4166647224609967 1101 118-119 1.2947629622856418 1101 120-121 1.3325105165745725 1101 122-123 1.3953373869917485 1101 124-125 1.5403147569400772 1102 1 0.15339683722520547 1102 2 0.3447031724784537 1102 3 0.48355771715272056 1102 4 0.5758879016094482 1102 5 0.7528157994892624 1102 6 0.12294016901271476 1102 7 -0.346944096216113 1102 8 0.357735898288162 1102 9 0.6186302909345329 1102 10-11 0.7408996362933493 1102 12-13 0.8324428879190293 1102 14-15 0.9925802176315344 1102 16-17 0.7713689364383356 1102 18-19 0.9108378043392875 1102 20-21 1.464115877199454 1102 22-23 1.3028901360294398 1102 24-25 1.501268368805519 1102 26-27 1.0573972834792684 1102 28-29 1.5368934692770964 1102 30-31 1.3364645867146763 1102 32-33 1.211801829698551 1102 34-35 1.6991987721011057 1102 36-37 1.7209676948775368 1102 38-39 1.6624208521204054 1102 40-41 1.4819981734921086 1102 42-43 1.2841690033565953 1102 44-45 1.4914867121421764 1102 46-47 1.1495261127361118 1102 48-49 0.7145201935629899 1102 50-51 1.1180344877323733 1102 52-53 0.947640428986098 1102 54-55 0.8111309153529547 1102 56-57 0.8288544929842168 1102 58-59 0.9265702926235164 1102 60-61 0.8104654936195139 1102 62-63 0.7325220596359117 1102 64-65 0.8356003828240368 1102 66-67 0.7732290820309302 1102 68-69 0.6708038322147409 1102 70-71 0.7173055455296584 1102 72-73 0.7884041760842173 1102 74-75 0.5480055682783522 1102 76-77 0.7237763126731025 1102 78-79 0.561080715224648 1102 80-81 0.17424649264239633 1102 82-83 0.37184248289494803 1102 84-85 0.0943881201265171 1102 86-87 0.5594459400972269 1102 88-89 0.7434375063149723 1102 90-91 0.2072810834366443 1102 92-93 0.7116685186829166 1102 94-95 0.6956722597635938 1102 96-97 0.7621432801005739 1102 98-99 0.7302390416828146 1102 100-101 0.754561236342294 1102 102-103 0.6353051612152889 1102 104-105 0.8740152510424934 1102 106-107 0.8112701449439115 1102 108-109 0.45291947682274625 1102 110-111 0.6929439697185487 1102 112-113 0.3455151304457722 1102 114-115 0.45390859080442425 1102 116-117 0.4616582101103788 1102 118-119 0.7435204743391992 1102 120-121 1.020259299793274 1102 122-123 0.8617262314493956 1102 124-125 0.9999061926277619 1103 1 0.5160243323899678 1103 2 0.595378509120426 1103 3 0.5175811361214464 1103 4 0.6090090471044647 1103 5 0.6432176259316194 1103 6 0.801587807957354 1103 7 0.8508319228328958 1103 8 0.8504330938506968 1103 9 1.0074577003422647 1103 10-11 1.0051794368626332 1103 12-13 0.9934742987134655 1103 14-15 1.3894020756397794 1103 16-17 1.2684878650227986 1103 18-19 1.2130779787727448 1103 20-21 1.5689877206107639 1103 22-23 1.5589736396604295 1103 24-25 1.5951693559237157 1103 26-27 1.5310202672766664 1103 28-29 1.4576341272342503 1103 30-31 1.2902258081137887 1103 32-33 1.2966754064967105 1103 34-35 1.7481500730535586 1103 36-37 1.7617449364204347 1103 38-39 1.8612938085485382 1103 40-41 1.5285518126922 1103 42-43 1.4442055413268804 1103 44-45 1.2901541709970488 1103 46-47 1.2655976879286115 1103 48-49 1.2145958011606979 1103 50-51 1.3369900993442485 1103 52-53 1.2022115525857018 1103 54-55 1.1460648185752262 1103 56-57 1.1925816624547174 1103 58-59 1.1906896408700618 1103 60-61 1.0968040103877534 1103 62-63 1.0158261230596253 1103 64-65 1.0738995653952905 1103 66-67 1.0265176133961091 1103 68-69 1.1081763370499758 1103 70-71 1.1763070690854747 1103 72-73 1.1696428862619364 1103 74-75 1.2448514884330777 1103 76-77 1.177034509768312 1103 78-79 1.1691773424187524 1103 80-81 1.0849427439652466 1103 82-83 1.17981457992812 1103 84-85 1.0218418888744623 1103 86-87 1.0185873890261767 1103 88-89 1.009771940501011 1103 90-91 0.9619097965337495 1103 92-93 1.0975772239341666 1103 94-95 1.0921610109952091 1103 96-97 1.0847096961864047 1103 98-99 1.1802680074894738 1103 100-101 1.1178230142498258 1103 102-103 1.0216837055352563 1103 104-105 1.0238049638476632 1103 106-107 1.1907238087763403 1103 108-109 1.3499707805121233 1103 110-111 1.3387206638761242 1103 112-113 1.3195209418532805 1103 114-115 1.4042103369636045 1103 116-117 1.3139690884813646 1103 118-119 1.3656249200203305 1103 120-121 1.3980261045226854 1103 122-123 1.3741838090868796 1103 124-125 1.3459684394620481 1104 1 0.7108298354797462 1104 2 0.8190843865421229 1104 3 0.7474190938568412 1104 4 0.7138914866946848 1104 5 0.6633189333559883 1104 6 0.6051617111374625 1104 7 0.7432301021563603 1104 8 0.7803833682695185 1104 9 1.0951369964340216 1104 10-11 1.0415575745442354 1104 12-13 0.8345579536848291 1104 14-15 1.0343176499347493 1104 16-17 1.0167291209166436 1104 18-19 1.310308897767868 1104 20-21 1.5051518749080515 1104 22-23 1.372406261626253 1104 24-25 1.7748548777643123 1104 26-27 1.734428411391029 1104 28-29 1.58925914348724 1104 30-31 1.5144017389821443 1104 32-33 1.5457732588785404 1104 34-35 1.745935817645595 1104 36-37 1.9407375678300909 1104 38-39 1.9669710405111474 1104 40-41 1.6189189462472555 1104 42-43 1.5973007513154585 1104 44-45 1.4868166845982316 1104 46-47 1.524824785761318 1104 48-49 1.3626708702786559 1104 50-51 1.2980114839959498 1104 52-53 1.3620104774934916 1104 54-55 1.2569155254945557 1104 56-57 1.286354865729951 1104 58-59 1.1862393168368541 1104 60-61 1.1572887471236122 1104 62-63 1.242117936003158 1104 64-65 1.246526372898348 1104 66-67 1.2059440498178517 1104 68-69 1.0665115950789499 1104 70-71 1.0115918299441802 1104 72-73 0.9963669216900222 1104 74-75 0.9728853647872064 1104 76-77 0.972968547454709 1104 78-79 1.0385085791169644 1104 80-81 1.0801392382818307 1104 82-83 1.0213348200274872 1104 84-85 1.1197040011124493 1104 86-87 1.1064603308849712 1104 88-89 1.02416481450787 1104 90-91 1.0159068119723749 1104 92-93 1.0097492985678755 1104 94-95 1.0246672812258986 1104 96-97 0.9003465223718443 1104 98-99 1.0218101677336477 1104 100-101 1.0450211877564293 1104 102-103 0.967827085950919 1104 104-105 0.9726067766543274 1104 106-107 0.9681188572615653 1104 108-109 0.9739426722569746 1104 110-111 1.0048648826036697 1104 112-113 0.9509945487158014 1104 114-115 0.8555790521907696 1104 116-117 0.9856288433521243 1104 118-119 0.9445852379287274 1104 120-121 0.9940284261333403 1104 122-123 1.0346101847737827 1104 124-125 1.2561768968313203 1105 1 0.5009742394480838 1105 2 0.8398481253251191 1105 3 0.8572512607729124 1105 4 0.9008482713130945 1105 5 1.0486680523708571 1105 6 0.860576859950136 1105 7 0.8777081871158785 1105 8 0.8165486014773933 1105 9 0.8074173799661253 1105 10-11 1.0252037999373655 1105 12-13 0.9481463368743164 1105 14-15 1.11655699472567 1105 16-17 1.0562386714303145 1105 18-19 1.2043031714904728 1105 20-21 1.5048227966044223 1105 22-23 1.609567169265155 1105 24-25 1.5918190060248634 1105 26-27 1.4884233275248775 1105 28-29 1.368818247840018 1105 30-31 1.3152432347170375 1105 32-33 1.4606418039052755 1105 34-35 1.934973510809833 1105 36-37 1.9280442776264906 1105 38-39 1.9776719111673131 1105 40-41 1.6080999852907425 1105 42-43 1.2769699473189036 1105 44-45 1.3820923168064638 1105 46-47 1.3643561278717513 1105 48-49 1.3089189997282986 1105 50-51 1.2274032465204527 1105 52-53 1.1277555359655125 1105 54-55 1.1258516672986403 1105 56-57 1.0526793539423345 1105 58-59 1.0775294801352686 1105 60-61 1.04893979437988 1105 62-63 1.0335619630902393 1105 64-65 1.1784865207285158 1105 66-67 0.8926838631910918 1105 68-69 0.76448100874644 1105 70-71 0.9231173172949738 1105 72-73 0.9863739630209984 1105 74-75 0.9832149595390867 1105 76-77 1.0865024516522972 1105 78-79 1.1244225791472005 1105 80-81 0.9337626895669047 1105 82-83 0.9476930789166929 1105 84-85 0.9157198548140002 1105 86-87 0.8553676019724321 1105 88-89 1.0193394873819912 1105 90-91 0.9263279188343354 1105 92-93 0.8433931306642535 1105 94-95 0.8188384069823087 1105 96-97 0.9359206742630555 1105 98-99 0.7617715474818212 1105 100-101 0.6754196464733191 1105 102-103 0.5829963046277058 1105 104-105 0.8207987039384186 1105 106-107 0.7884238388457732 1105 108-109 0.7782799557895821 1105 110-111 0.8372223176949447 1105 112-113 0.828663074596161 1105 114-115 0.837266553135489 1105 116-117 0.7328397079116833 1105 118-119 0.8965067403144644 1105 120-121 0.74854514394098 1105 122-123 0.7877888607516361 1105 124-125 1.017437672964867 1106 1 0.2864427116342192 1106 2 0.59340796372501 1106 3 0.40018329396920294 1106 4 0.3993300862065894 1106 5 0.2883445210111617 1106 6 0.335058104250578 1106 7 0.24457405189551196 1106 8 0.2445099539867286 1106 9 0.4057708114210854 1106 10-11 0.6459772082629343 1106 12-13 0.6439616481277284 1106 14-15 0.8816121180909491 1106 16-17 0.7247444817998243 1106 18-19 0.6845008539656234 1106 20-21 0.9820872741296753 1106 22-23 1.0658808196851979 1106 24-25 1.219918740087735 1106 26-27 1.2971084416689607 1106 28-29 1.3488990733659065 1106 30-31 1.1389716910080807 1106 32-33 1.0353035237338943 1106 34-35 1.3438187221585238 1106 36-37 1.27999448319283 1106 38-39 1.4502971359656982 1106 40-41 1.2473514385199422 1106 42-43 1.1062575465995508 1106 44-45 0.871955374472023 1106 46-47 0.5868129130893109 1106 48-49 0.6652451318715364 1106 50-51 0.690532172531853 1106 52-53 0.6744362254660885 1106 54-55 0.43329370370451414 1106 56-57 0.5094789000151785 1106 58-59 0.5401467191781038 1106 60-61 0.5314532822827971 1106 62-63 0.4856923257734529 1106 64-65 0.5257532143964561 1106 66-67 0.32939420315247503 1106 68-69 0.43975146730966586 1106 70-71 0.5613492175227037 1106 72-73 0.6160224496757429 1106 74-75 0.6910222773950814 1106 76-77 0.7183721755769596 1106 78-79 0.598040610397355 1106 80-81 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2216 34-35 -6.144227400323853 2216 36-37 -5.182530721603975 2216 38-39 -5.979689879702882 2216 40-41 -4.681654377993034 2216 42-43 -6.277994346090772 2216 44-45 -6.172290974971787 2216 46-47 -4.480031627230289 2216 48-49 -3.1596472729346132 2216 50-51 -2.88209632253794 2216 52-53 -3.15477863154765 2216 54-55 -2.720198291146275 2216 56-57 -2.7045677756787434 2216 58-59 -2.695418160459429 2216 60-61 -2.9314331240182483 2216 62-63 -2.964781605106271 2216 64-65 -3.007774522150175 2216 66-67 -2.8222617304309736 2216 68-69 -2.6496904290594507 2216 70-71 -2.5657205255344664 2216 72-73 -2.5170023875192165 2216 74-75 -2.4159331612476898 2216 76-77 -2.4895952951387272 2216 78-79 -2.6577960775462515 2216 80-81 -2.663460815219622 2216 82-83 -2.4439943652917577 2216 84-85 -2.4311746722275913 2216 86-87 -2.5051727680217155 2216 88-89 -2.6125228729467587 2216 90-91 -2.5078515221728352 2216 92-93 -2.6047438164222747 2216 94-95 -2.683098906404588 2216 96-97 -2.5386056747380863 2216 98-99 -2.666657516451444 2216 100-101 -2.6199388815532743 2216 102-103 -2.492903285977768 2216 104-105 -2.721741389021844 2216 106-107 -2.6479737971316943 2216 108-109 -2.7120123149648734 2216 110-111 -2.8674537835147724 2216 112-113 -2.888231375491568 2216 114-115 -2.908293209230994 2216 116-117 -2.945021821315393 2216 118-119 -3.034752301763902 2216 120-121 -3.098953470889569 2216 122-123 -3.1285045797014703 2216 124-125 -3.337494684593544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73436.0 3 13891.0 4 10560.0 5 9419.0 6 9285.0 7 8435.0 8 8234.0 9 8738.0 10 9402.0 11 9741.0 12 10033.0 13 11189.0 14 13101.0 15 11279.0 16 8048.0 17 7283.0 18 6563.0 19 6349.0 20 6409.0 21 6976.0 22 8376.0 23 12627.0 24 17468.0 25 18371.0 26 23710.0 27 25359.0 28 29375.0 29 33988.0 30 39128.0 31 45806.0 32 56776.0 33 72023.0 34 95166.0 35 137162.0 36 209192.0 37 269950.0 38 88650.0 39 237.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.5526636378369 18.891557276735572 12.812103583742672 22.743675501684855 2 15.2141904209769 21.2861204048419 39.413342460203815 24.086346713977385 3 19.08139352134812 27.334881690386798 27.922256177420763 25.661468610844317 4 12.21965482653315 17.523826021843664 36.11217439167158 34.14434475995161 5 13.4918206623105 39.90355800141666 32.257284842693224 14.347336493579615 6 31.148073413334508 37.01356890864123 17.27631416280595 14.562043515218312 7 28.203872712617116 34.2991831848109 20.041099201066174 17.455844901505806 8 26.097636783343287 36.04298281455716 19.10737671426626 18.752003687833295 9 27.001493323349905 17.75846437746297 18.811892944501178 36.42814935468594 10-11 24.563309550999314 28.281420793652458 26.07881346757605 21.076456187772177 12-13 25.550115247607742 25.80484738422854 26.646364461828597 21.998672906335127 14-15 22.699478001491425 27.082065011430178 24.54622454842501 25.672232438653385 16-17 22.243938052247238 30.316008991401578 24.90387688696299 22.53617606938819 18-19 22.271232362897624 28.76438916193707 27.297297958023336 21.667080517141965 20-21 23.351841206222502 27.901613740296987 27.066943365070763 21.67960168840975 22-23 22.91816181983845 27.74909993961764 26.991124945388712 22.341613295155195 24-25 22.534568826987698 28.04850235802516 27.104349292976636 22.312579522010505 26-27 22.458732387478957 28.1195730382603 26.983185587247032 22.438508987013712 28-29 22.228027171352668 28.165256192078314 27.334512094995667 22.272204541573352 30-31 22.306903045017307 28.096493997412896 27.117109817868908 22.47949313970089 32-33 22.320982652701282 28.27567780893873 26.710683938294327 22.69265560006566 34-35 22.67648527732278 28.065463613281317 26.856531168908276 22.401519940487624 36-37 22.85828243485647 27.89342473638035 26.725053226860986 22.523239601902198 38-39 22.56063447495521 27.918539394510855 26.908401345221005 22.612424785312925 40-41 22.40194405565582 28.078533477290886 26.9151250086888 22.604397458364495 42-43 22.63861588775777 28.091117882004834 26.595607208068074 22.674659022169322 44-45 22.593632523844068 28.21631661835624 26.422200419757598 22.767850438042096 46-47 22.622870094538293 28.401510098170352 26.069833309468848 22.90578649782251 48-49 22.83811694796433 28.461229196087974 25.769902366966214 22.930751488981485 50-51 22.85153705732529 28.731340547000535 25.65041571775267 22.766706677921505 52-53 22.957879775237735 28.36149846165666 25.83033871491582 22.850283048189784 54-55 22.73578081564038 28.35174783823271 25.84962684912005 23.062844497006864 56-57 22.739346840931823 28.747604226619206 25.559374668223807 22.953674264225164 58-59 22.80537126976239 28.22829188634577 25.663280433406193 23.30305641048565 60-61 22.92377727759278 28.260502866362714 25.91397943343281 22.9017404226117 62-63 22.752303576424872 28.515375165352058 25.73239566010009 22.999925598122978 64-65 22.7058684640871 28.272732797637218 25.871670440010814 23.14972829826487 66-67 22.73622576591817 28.465970484307956 25.816032961913454 22.981770787860416 68-69 22.675678271727836 28.392773251600172 25.97301757786917 22.958530898802817 70-71 22.66187703603538 28.51853455742248 25.827440588278023 22.99214781826411 72-73 22.672722859333224 28.436269399287855 25.70703604552273 23.183971695856187 74-75 22.610783420116153 28.7705825449542 25.589127876317896 23.029506158611753 76-77 22.96112802216147 28.44652483735823 25.703302567483938 22.88904457299637 78-79 22.885988261679476 28.493295830682097 25.781105396931387 22.839610510707047 80-81 22.633912575199115 28.924410787463007 25.774691467969173 22.6669851693687 82-83 22.717129153317313 28.764693022433903 25.52358461902736 22.994593205221424 84-85 22.718659528474195 28.53017492762278 25.55542750578843 23.195738038114595 86-87 22.960131259023534 28.345194747683305 25.618873862956136 23.075800130337026 88-89 22.883492131018482 28.173395266700823 25.74045336538277 23.202659236897922 90-91 22.67054798061299 28.460523907524692 25.837702955135754 23.031225156726563 92-93 22.63584387536543 28.3471381189846 25.792533925826795 23.224484079823174 94-95 22.748071871681663 28.23223467277706 25.826754876208575 23.1929385793327 96-97 22.89395859885626 28.224162880218064 25.65987940878661 23.221999112139073 98-99 22.663441655930793 28.376229677944774 25.582050761790047 23.378277904334382 100-101 22.56160532706171 28.270259113104018 25.799480104370286 23.368655455463987 102-103 22.691804000041905 27.920390295690193 26.03376323132423 23.354042472943668 104-105 22.56376945212751 28.33577794540832 25.92060598441036 23.17984661805381 106-107 22.55138784460387 28.397094573051078 25.810406887589842 23.241110694755207 108-109 22.55326579290162 28.49877945290155 25.5480937463986 23.399861007798233 110-111 22.35260879267709 28.508537425644793 25.88572320601959 23.25313057565853 112-113 22.52571181119411 28.513272358362407 25.724034126427025 23.236981704016454 114-115 22.281008706220078 28.795552249543388 25.694419707557614 23.229019336678924 116-117 22.183330015680276 28.932169710176815 25.77554505547465 23.10895521866826 118-119 22.259531268007 29.00239918700039 25.516837962332417 23.2212315826602 120-121 22.25738160859489 28.91092200087446 25.58041507710231 23.251281313428333 122-123 22.2735352561752 28.839938955183747 25.553611527272857 23.332914261368202 124-125 22.324119497162915 29.03888366478086 25.294397505096416 23.342599332959807 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5622.0 1 5108.0 2 5961.5 3 6609.0 4 4731.0 5 2898.0 6 2012.5 7 1963.0 8 2416.0 9 2463.5 10 1782.5 11 1257.0 12 1110.5 13 1130.0 14 1218.0 15 1261.0 16 1321.5 17 1452.5 18 1602.0 19 1727.0 20 1958.5 21 2266.0 22 2736.0 23 3332.5 24 4088.0 25 5341.0 26 6779.0 27 8455.5 28 10441.0 29 12031.5 30 13932.5 31 16363.5 32 18777.5 33 21146.0 34 23185.0 35 24408.5 36 27082.5 37 30768.0 38 32666.5 39 33647.0 40 34700.0 41 36634.0 42 39390.0 43 43005.0 44 46039.5 45 47594.5 46 48768.0 47 50116.0 48 54300.5 49 55300.0 50 52915.0 51 52465.5 52 52715.0 53 53702.0 54 51713.5 55 47381.5 56 43416.0 57 39867.5 58 37253.5 59 34899.5 60 31374.5 61 26514.0 62 21793.0 63 16648.5 64 12298.0 65 10155.0 66 8480.0 67 7051.5 68 5692.0 69 4347.5 70 3257.5 71 2564.0 72 2175.5 73 1822.5 74 1371.0 75 1017.5 76 780.0 77 600.0 78 425.5 79 260.0 80 174.0 81 119.5 82 87.5 83 64.0 84 49.5 85 44.0 86 28.0 87 20.0 88 21.5 89 17.0 90 13.5 91 8.5 92 5.5 93 6.5 94 8.0 95 7.0 96 4.5 97 4.0 98 3.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011105407076030131 2 0.0045399462889431355 3 0.007682986027442229 4 0.007473450044875623 5 0.014038910831962619 6 4.1907196513321247E-4 7 0.0053780902192095605 8 0.0 9 0.0020255144981438603 10-11 0.0 12-13 0.002444586463277073 14-15 0.015191358736078953 16-17 0.025668157864409263 18-19 0.004085951660048822 20-21 0.006425770132042592 22-23 0.001781055851816153 24-25 0.009394196551736182 26-27 0.01571519869249547 28-29 4.539946288943135E-4 30-31 0.0013270612229218394 32-33 0.006984532752220209 34-35 0.006984532752220209 36-37 0.008870356595319664 38-39 0.0 40-41 0.020569448955288512 42-43 0.008171903320097644 44-45 0.013584916203068304 46-47 0.0027938131008880835 48-49 0.02119805690298833 50-51 0.0038065703499600137 52-53 0.0 54-55 0.014632596115901338 56-57 0.0032827303935434983 58-59 4.539946288943135E-4 60-61 0.0029684264196935886 62-63 0.022315582143343567 64-65 0.014108756159484821 66-67 0.0015365972054884457 68-69 0.017286718561745015 70-71 0.0021652051531882645 72-73 0.009952959171913797 74-75 0.0 76-77 0.004260564978854327 78-79 9.778345853108292E-4 80-81 0.0025144317907992747 82-83 0.0012572158953996374 84-85 0.0 86-87 0.004330410306376529 88-89 0.01372460685811271 90-91 0.018229630483294745 92-93 0.0031779624022601947 94-95 0.020604371619049613 96-97 0.014283369478290325 98-99 5.936852839387177E-4 100-101 0.007089300743503512 102-103 0.0 104-105 0.0010476799128330313 106-107 3.4922663761101044E-5 108-109 0.0 110-111 6.635306114609197E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 6.984532752220209E-5 122-123 0.0 124-125 0.023537875374982104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1431735.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.953841430352604 #Duplication Level Percentage of deduplicated Percentage of total 1 69.29568075178013 29.76515682820301 2 12.65831103311166 10.874461697847224 3 5.786592431047588 7.4566912111589 4 3.334032959365473 5.728380922406151 5 2.1763100119270233 4.674043757780107 6 1.4100585562656298 3.6340458980007475 7 1.006416969610905 3.026063244783797 8 0.7639832780691376 2.6252813265298176 9 0.5495857995855242 2.1246139158993143 >10 2.8404256709802724 20.94941843525997 >50 0.1242015049853281 3.5937448064298176 >100 0.051537836181706105 3.6880536013363585 >500 0.0018526569403681265 0.5148743988959533 >1k 8.421167910764211E-4 0.8757277783666594 >5k 1.6842335821528421E-4 0.46944217710212743 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6489 0.45322633029156933 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3884 0.2712792520962329 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2729 0.19060789880808948 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2095 0.14632596115901336 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1712 0.11957520071800996 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1685 0.11768937687491052 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.984532752220209E-5 0.0 14-15 0.0 0.0 0.0 6.984532752220209E-5 0.0 16-17 0.0 0.0 0.0 1.7461331880550522E-4 0.0 18-19 0.0 0.0 0.0 3.841493013721115E-4 0.0 20-21 0.0 0.0 0.0 6.984532752220209E-4 0.0 22-23 0.0 0.0 0.0 0.00167628786053285 0.0 24-25 0.0 0.0 0.0 0.0028636584284102854 0.0 26-27 0.0 0.0 0.0 0.0042954876426154285 0.0 28-29 0.0 0.0 0.0 0.00635592480452039 0.0 30-31 0.0 0.0 0.0 0.012257854980146466 0.0 32-33 0.0 0.0 0.0 0.020604371619049613 0.0 34-35 0.0 0.0 0.0 0.031081170747379927 0.0 36-37 0.0 0.0 0.0 0.0468312921036365 0.0 38-39 0.0 0.0 0.0 0.07571233503406705 0.0 40-41 0.0 0.0 0.0 0.10934286023600737 0.0 42-43 0.0 0.0 0.0 0.1410875615948482 0.0 44-45 0.0 0.0 0.0 0.17541654007201052 0.0 46-47 0.0 0.0 0.0 0.2216890695554694 0.0 48-49 0.0 0.0 0.0 0.27099987078614407 0.0 50-51 0.0 0.0 0.0 0.32533953559841733 0.0 52-53 0.0 0.0 0.0 0.3755583260868806 0.0 54-55 0.0 0.0 0.0 0.43038690819180925 0.0 56-57 0.0 0.0 0.0 0.4923397137040025 0.0 58-59 0.0 0.0 0.0 0.5667599101789087 0.0 60-61 0.0 0.0 0.0 0.6582922118967547 0.0 62-63 0.0 0.0 0.0 0.7440273514302577 0.0 64-65 0.0 0.0 0.0 0.8307054028853105 0.0 66-67 0.0 0.0 0.0 0.9311429838622372 0.0 68-69 0.0 0.0 0.0 1.0433145798628936 0.0 70-71 0.0 0.0 0.0 1.1766143874390163 0.0 72-73 0.0 0.0 0.0 1.3190639329205474 0.0 74-75 0.0 0.0 0.0 1.4627706942974783 0.0 76-77 0.0 0.0 0.0 1.6127984578151684 0.0 78-79 0.0 0.0 0.0 1.7870974726468236 0.0 80-81 0.0 0.0 0.0 1.989160005168554 0.0 82-83 0.0 0.0 0.0 2.197892766468655 0.0 84-85 0.0 0.0 0.0 2.4149371217438977 0.0 86-87 0.0 0.0 0.0 2.6432614974139765 0.0 88-89 0.0 0.0 0.0 2.89267916199576 0.0 90-91 0.0 0.0 0.0 3.180511756714755 0.0 92-93 0.0 0.0 0.0 3.5008922740590958 0.0 94-95 0.0 0.0 0.0 3.817116994415866 0.0 96-97 0.0 0.0 0.0 4.131840040230909 0.0 98-99 0.0 0.0 0.0 4.4801586885841305 0.0 100-101 0.0 3.4922663761101044E-5 0.0 4.880337492622587 0.0 102-103 0.0 6.984532752220209E-5 0.0 5.314111899199223 0.0 104-105 0.0 6.984532752220209E-5 0.0 5.751029345514358 0.0 106-107 0.0 6.984532752220209E-5 0.0 6.186968957244183 0.0 108-109 0.0 6.984532752220209E-5 0.0 6.650776854655366 0.0 110-111 0.0 6.984532752220209E-5 0.0 7.174197739106749 0.0 112-113 0.0 6.984532752220209E-5 0.0 7.740783035966851 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7095 0.0 42.765373 1 GTACATG 8560 0.0 36.558342 1 ATCAACG 8280 0.0 35.854588 3 TCAACGC 8275 0.0 35.58867 4 AACGCAG 8300 0.0 35.40608 6 CAACGCA 8360 0.0 35.369152 5 GGTATCA 4500 0.0 35.29988 1 TATCAAC 8710 0.0 34.35772 2 TACATGG 9000 0.0 33.779476 2 ACATGGG 9215 0.0 31.700102 3 ACGCAGA 9340 0.0 31.399956 7 CGCAGAG 9240 0.0 30.779446 8 ATGGGAG 1545 0.0 29.650822 5 GAGTACT 3725 0.0 29.080488 12-13 CATGGGG 4200 0.0 28.472218 4 GCAGAGT 9720 0.0 27.729166 9 CATGGGA 5350 0.0 26.911388 4 GTACTTT 4115 0.0 26.252058 14-15 GAACAAA 2300 0.0 23.797672 1 AGTACTT 4220 0.0 23.6243 12-13 >>END_MODULE