FastQCFastQC Report
Thu 26 May 2016
SRR1364525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431735
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117730.8222890409188851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92700.6474661861308133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70590.49303816697922453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27110.18935068291268986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26260.18341383007330267No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.1503071448277789No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.14604657984892455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20360.14220508683520344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53700.054.961551
GTATCAA90300.048.8620871
ATCAACG114150.038.145573
TCAACGC115100.037.7272264
TATCAAC116400.037.3570442
CAACGCA117500.036.964675
AACGCAG118700.036.5408486
ACGCAGA137400.031.3944637
GTACATG72850.031.3470941
CGCAGAG136900.031.2918228
TACATGG76650.029.2975852
GCAGAGT143150.028.9686369
GAGTACT93250.027.75506212-13
ACATGGG79650.026.9227473
AGTACTT97400.026.02270512-13
CAGAGTA138200.024.98996510-11
GTACTTT104100.024.94797514-15
TATCACG1200.001279649124.8194072
AGAGTAC136650.024.57682410-11
TACTTTT107600.023.7770514-15
Produced by FastQC (version 0.11.5)