##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364525_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1431735 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.151640492130177 31.0 30.0 34.0 26.0 34.0 2 30.043084090282072 31.0 30.0 34.0 26.0 34.0 3 30.29015006268618 31.0 30.0 34.0 26.0 34.0 4 33.86352083311507 35.0 35.0 37.0 30.0 37.0 5 33.30905998665954 35.0 33.0 37.0 27.0 37.0 6 33.31016144747457 36.0 35.0 37.0 27.0 37.0 7 33.10992397336099 36.0 33.0 37.0 26.0 37.0 8 33.03516782086071 36.0 33.0 37.0 26.0 37.0 9 34.25992729101405 38.0 34.0 39.0 25.0 39.0 10-11 34.21053477075017 38.0 34.0 39.0 25.0 39.0 12-13 34.08469095188705 38.0 34.0 39.0 25.0 39.0 14-15 35.02476191473981 39.0 34.0 41.0 25.0 41.0 16-17 35.04922873297083 39.0 34.0 41.0 25.0 41.0 18-19 35.09013539516741 39.0 34.0 41.0 25.0 41.0 20-21 35.11143053707565 39.0 34.0 41.0 25.0 41.0 22-23 35.05422372156859 39.0 34.0 41.0 25.0 41.0 24-25 34.990965157658366 39.0 34.0 41.0 25.0 41.0 26-27 34.83515804251485 39.0 34.0 41.0 24.0 41.0 28-29 34.659655941916625 39.0 33.5 41.0 24.0 41.0 30-31 34.43147859066098 38.0 33.0 40.5 23.5 41.0 32-33 34.14131106664291 38.0 33.0 40.0 22.0 41.0 34-35 33.90753386625318 38.0 33.0 40.0 20.5 41.0 36-37 33.65680485564717 38.0 33.0 40.0 18.0 41.0 38-39 33.45711217508827 38.0 33.0 40.0 16.0 41.0 40-41 33.431707683335254 38.0 33.0 40.0 15.5 41.0 42-43 33.24526151836758 38.0 32.5 40.0 14.0 41.0 44-45 33.01390445857648 38.0 32.0 40.0 9.0 41.0 46-47 32.667888959898306 38.0 31.5 40.0 6.5 41.0 48-49 32.312388116515976 38.0 31.0 40.0 2.0 41.0 50-51 32.12371179024051 37.0 31.0 40.0 2.0 41.0 52-53 31.926448330172832 37.0 31.0 40.0 2.0 41.0 54-55 31.73066140032897 37.0 30.0 40.0 2.0 41.0 56-57 31.502175681952316 36.0 30.0 40.0 2.0 41.0 58-59 31.2806542411829 36.0 30.0 40.0 2.0 41.0 60-61 31.01547004159289 35.5 30.0 39.5 2.0 41.0 62-63 30.81587689062571 35.0 29.5 39.0 2.0 41.0 64-65 30.663861329086735 35.0 29.5 39.0 2.0 41.0 66-67 30.35209029603942 35.0 29.0 39.0 2.0 41.0 68-69 30.074078303596686 35.0 29.0 38.0 2.0 40.0 70-71 29.765771249567834 35.0 29.0 37.0 2.0 40.0 72-73 29.483640478161114 35.0 29.0 37.0 2.0 39.5 74-75 29.15521727135259 35.0 29.0 36.0 2.0 39.0 76-77 28.859806458597436 34.0 28.5 36.0 2.0 39.0 78-79 28.59733470230175 34.0 28.0 35.5 2.0 37.5 80-81 28.34662594684072 34.0 28.0 35.0 2.0 37.0 82-83 28.082223665692325 34.0 27.5 35.0 2.0 37.0 84-85 27.87886829615816 34.0 27.0 35.0 2.0 36.0 86-87 27.675904409684755 34.0 27.0 35.0 2.0 36.0 88-89 27.500227695767723 34.0 26.5 35.0 2.0 36.0 90-91 27.366309058589756 34.0 26.0 35.0 2.0 35.0 92-93 27.26625213464782 34.0 26.0 35.0 2.0 35.0 94-95 27.15218249187175 34.0 26.0 35.0 2.0 35.0 96-97 27.037776893070294 34.0 26.0 35.0 2.0 35.0 98-99 26.940299007847123 34.0 25.0 35.0 2.0 35.0 100-101 26.83642573520938 34.0 25.0 35.0 2.0 35.0 102-103 25.54823623086675 31.5 23.5 34.0 2.0 35.0 104-105 26.480754120001258 33.0 25.0 35.0 2.0 35.0 106-107 26.632579353022734 34.0 25.0 35.0 2.0 35.0 108-109 26.623197030176676 34.0 25.0 35.0 2.0 35.0 110-111 26.532830446975176 34.0 25.0 35.0 2.0 35.0 112-113 26.44014499889994 34.0 25.0 35.0 2.0 35.0 114-115 26.315885970518288 34.0 24.0 35.0 2.0 35.0 116-117 26.177963799166747 34.0 24.0 35.0 2.0 35.0 118-119 26.03812297666817 34.0 24.0 35.0 2.0 35.0 120-121 25.826529699979396 33.5 23.0 35.0 2.0 35.0 122-123 25.52870014353215 33.0 19.5 35.0 2.0 35.0 124-125 23.763497784156982 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5895262126936416 1101 2 0.6958519967074359 1101 3 0.48747168253169804 1101 4 0.3525109815559304 1101 5 0.5052365696391021 1101 6 0.581374269593141 1101 7 0.7106041501097522 1101 8 0.5740965594413936 1101 9 0.696824218671388 1101 10-11 0.8025307510035731 1101 12-13 0.8179422959152163 1101 14-15 0.9108976589737452 1101 16-17 0.9201360712079136 1101 18-19 0.893379166133137 1101 20-21 1.0045832422507601 1101 22-23 1.0401620453452907 1101 24-25 0.9579369613506827 1101 26-27 1.0744760395151118 1101 28-29 1.1932256827331358 1101 30-31 1.192376181922718 1101 32-33 1.2140366917510903 1101 34-35 1.1663062179457242 1101 36-37 1.1866677316890986 1101 38-39 1.2487834868770378 1101 40-41 1.1283014509781495 1101 42-43 0.9372066303466653 1101 44-45 0.8665210343929886 1101 46-47 1.0229346928911696 1101 48-49 1.2402235598177427 1101 50-51 1.0901619440411352 1101 52-53 0.9904865057357881 1101 54-55 0.99940913222731 1101 56-57 0.9904615972580935 1101 58-59 0.9202555151531442 1101 60-61 0.8505960389053939 1101 62-63 0.9136610889556032 1101 64-65 0.8963287186056945 1101 66-67 0.9271596088812686 1101 68-69 1.0067049653386242 1101 70-71 1.0603476422627196 1101 72-73 0.994193592316762 1101 74-75 1.1313753513865663 1101 76-77 1.1130361517812908 1101 78-79 1.0581742563631593 1101 80-81 0.9317966680314953 1101 82-83 1.1289112975947724 1101 84-85 1.1619302046889253 1101 86-87 1.2384537823078325 1101 88-89 1.2576730402287026 1101 90-91 1.174352329219051 1101 92-93 1.0638046994671875 1101 94-95 1.1624177431201694 1101 96-97 1.2835162600913854 1101 98-99 1.2259542233006577 1101 100-101 1.1630442025265282 1101 102-103 1.0474802331251496 1101 104-105 1.1057522869542282 1101 106-107 1.0833476756572722 1101 108-109 1.0935739098291144 1101 110-111 0.9335920462002463 1101 112-113 1.0062242027017838 1101 114-115 1.247154274924764 1101 116-117 1.21805660910303 1101 118-119 1.2892296821414568 1101 120-121 1.3255987976011383 1101 122-123 1.2467917298233786 1101 124-125 1.4947774998493308 1102 1 0.21056567525333847 1102 2 -0.40129285168047346 1102 3 0.1193113644547843 1102 4 0.26087078321486956 1102 5 0.2929762127165816 1102 6 0.23652702420320537 1102 7 0.3216625784624654 1102 8 0.4812281546245387 1102 9 0.5052023006127584 1102 10-11 0.6589739793014502 1102 12-13 0.6559949888293204 1102 14-15 0.8017872232175094 1102 16-17 0.686318119970565 1102 18-19 0.7467221727825972 1102 20-21 0.6149527324593791 1102 22-23 0.8682502705583701 1102 24-25 0.803424587884507 1102 26-27 0.8862685354591733 1102 28-29 0.47528529930421115 1102 30-31 0.6723455624496495 1102 32-33 0.8578505299248889 1102 34-35 0.701680849483779 1102 36-37 0.46658569892169055 1102 38-39 -0.01111642857665629 1102 40-41 -0.3850705756196646 1102 42-43 -0.10365437095579466 1102 44-45 -0.43327959627675483 1102 46-47 -0.1170837016430255 1102 48-49 -0.41663611616424845 1102 50-51 -0.6297064379253712 1102 52-53 -0.7353429620794927 1102 54-55 -0.6276805325580099 1102 56-57 -0.8885297881588166 1102 58-59 -0.7611877131447287 1102 60-61 -0.8794950197514595 1102 62-63 -0.7350530074271866 1102 64-65 -0.6923980597230326 1102 66-67 -0.7341027588723108 1102 68-69 -0.5198545712562144 1102 70-71 -1.0083827921224895 1102 72-73 -0.7748704572228036 1102 74-75 -0.6375207480581686 1102 76-77 -1.0076279937297592 1102 78-79 -0.8717145766693122 1102 80-81 -1.00376019507204 1102 82-83 -1.2031643142234287 1102 84-85 -1.0998782865022676 1102 86-87 -0.9574200151923442 1102 88-89 -0.9987793282103894 1102 90-91 -1.044566620578724 1102 92-93 -0.8127719748924314 1102 94-95 -0.7440192013306195 1102 96-97 -0.9751023218157044 1102 98-99 -1.1075535343941993 1102 100-101 -0.8255157682250243 1102 102-103 -0.9379121074365102 1102 104-105 -0.8093220909449101 1102 106-107 -0.802393704047045 1102 108-109 -0.7137262067076868 1102 110-111 -0.6450234509091857 1102 112-113 -0.47428044734505903 1102 114-115 -0.5185124983768823 1102 116-117 -0.7861232917264331 1102 118-119 -0.49310985125433504 1102 120-121 -0.37669766287917383 1102 122-123 -0.5701386680012668 1102 124-125 -0.25479005721942727 1103 1 0.20889432540113262 1103 2 -0.15209750810495137 1103 3 0.2896346411033264 1103 4 0.24199260381845278 1103 5 0.3725838286710399 1103 6 0.39730047966428117 1103 7 0.3880861908406743 1103 8 0.36580739777310356 1103 9 0.6068029465274378 1103 10-11 0.6921065286573622 1103 12-13 0.562456872564681 1103 14-15 0.7987659983516195 1103 16-17 0.6565737717240197 1103 18-19 0.7291590866241435 1103 20-21 0.7923173230151619 1103 22-23 0.7260086592384454 1103 24-25 0.7119010776848924 1103 26-27 0.7154105317734434 1103 28-29 0.7193119569694346 1103 30-31 0.7634329890087841 1103 32-33 0.8224204447152488 1103 34-35 0.7341608699194992 1103 36-37 0.8549796475769469 1103 38-39 0.9024533676135391 1103 40-41 0.8326164705066716 1103 42-43 0.9071287777870367 1103 44-45 0.6770516174589645 1103 46-47 0.8799989626638052 1103 48-49 0.7379337530106511 1103 50-51 0.8158567759563198 1103 52-53 0.6290287536085728 1103 54-55 0.6506959225746449 1103 56-57 0.722902924500378 1103 58-59 0.8731237673501084 1103 60-61 0.8719562355855466 1103 62-63 0.8156518652144946 1103 64-65 0.7986302948366841 1103 66-67 0.6082064773427014 1103 68-69 0.6312324447639277 1103 70-71 0.7074863645391929 1103 72-73 0.585567040496425 1103 74-75 0.6631706680276608 1103 76-77 0.6878522000446452 1103 78-79 0.7464597564329019 1103 80-81 0.6816584862767172 1103 82-83 0.7595052611880213 1103 84-85 0.764383039812369 1103 86-87 0.8474421349372072 1103 88-89 0.9189494560468106 1103 90-91 0.7921633907339078 1103 92-93 0.6432940808313923 1103 94-95 0.5591118427156481 1103 96-97 0.5540386481510886 1103 98-99 0.6220171330509743 1103 100-101 0.6552619978954759 1103 102-103 0.4660510763389887 1103 104-105 0.3990938443541481 1103 106-107 0.6152598147282724 1103 108-109 0.6492947216578244 1103 110-111 0.5200785680303426 1103 112-113 0.493653150253742 1103 114-115 0.6238390461620362 1103 116-117 0.6451907979717717 1103 118-119 0.47769215947441523 1103 120-121 0.5993089559216855 1103 122-123 0.6228570806393918 1103 124-125 0.9091135299790558 1104 1 0.3582028036426621 1104 2 0.47586652318290135 1104 3 0.22455704592926296 1104 4 0.010579817338197017 1104 5 0.28702169778489406 1104 6 0.36520938766896194 1104 7 0.3934768356222662 1104 8 0.2208812376521152 1104 9 0.3079853939875221 1104 10-11 0.4205693982844636 1104 12-13 0.441258360311636 1104 14-15 0.5174632830765589 1104 16-17 0.4642465281276955 1104 18-19 0.32464901148402703 1104 20-21 0.37063971124594985 1104 22-23 0.3989035340733409 1104 24-25 0.3912200519333169 1104 26-27 0.4771245502299948 1104 28-29 0.6255489118100854 1104 30-31 0.453629915312618 1104 32-33 0.5454632644884541 1104 34-35 0.6854138895531037 1104 36-37 0.6993672564719517 1104 38-39 0.8771302539863512 1104 40-41 0.848568241055581 1104 42-43 0.8915322205115217 1104 44-45 0.8112001235529789 1104 46-47 0.8084548178445701 1104 48-49 0.8261035257083762 1104 50-51 0.6211658991847457 1104 52-53 0.7084390225869228 1104 54-55 0.5020700697206131 1104 56-57 0.5707025910575574 1104 58-59 0.6067446074412572 1104 60-61 0.7059544443354682 1104 62-63 0.7799606300218223 1104 64-65 0.6772683973091915 1104 66-67 0.739773538871372 1104 68-69 0.450648517768915 1104 70-71 0.2140709621837047 1104 72-73 0.22208121138886838 1104 74-75 0.5506137184221807 1104 76-77 0.6086952423982162 1104 78-79 0.6641518581663277 1104 80-81 0.6710049751186133 1104 82-83 0.5985045777072138 1104 84-85 0.588570967950222 1104 86-87 0.5049613647852134 1104 88-89 0.38741192790347867 1104 90-91 0.49840821544118086 1104 92-93 0.4316903688206075 1104 94-95 0.46514563523859564 1104 96-97 0.3330921427140332 1104 98-99 0.39875500490217064 1104 100-101 0.38134006269527276 1104 102-103 0.24303862398589615 1104 104-105 0.38594443065920814 1104 106-107 0.3331991970806065 1104 108-109 0.33263652365198126 1104 110-111 0.40528063113298884 1104 112-113 0.28682418041928415 1104 114-115 0.31181476676808373 1104 116-117 0.36515699176210603 1104 118-119 0.3553811731406107 1104 120-121 0.3440212823569091 1104 122-123 0.2944933114523174 1104 124-125 0.6802766278322885 1105 1 0.08914964222361377 1105 2 -0.15306793071716385 1105 3 0.05029733040407436 1105 4 0.07070447031016869 1105 5 -0.05679742440423752 1105 6 0.22047854537966316 1105 7 0.2814541723616415 1105 8 0.35738795651784017 1105 9 0.3025652066553661 1105 10-11 0.33836185914775996 1105 12-13 0.37523706500905263 1105 14-15 0.3179429501311901 1105 16-17 0.29108237568225803 1105 18-19 0.2873964027905629 1105 20-21 0.3648948543147057 1105 22-23 0.3966210847482543 1105 24-25 0.45816119194874005 1105 26-27 0.46380711342562364 1105 28-29 0.5458899188085837 1105 30-31 0.5109676371964156 1105 32-33 0.511482859290723 1105 34-35 0.5787531557431222 1105 36-37 0.6594095926318957 1105 38-39 0.6382754590792885 1105 40-41 0.3066738962898441 1105 42-43 0.371109526515923 1105 44-45 0.3844025687464807 1105 46-47 0.3260499576886744 1105 48-49 0.4155885764210723 1105 50-51 0.32335192384326206 1105 52-53 0.4226661640836724 1105 54-55 0.27708663385341126 1105 56-57 0.2085917537060027 1105 58-59 0.12306909565364066 1105 60-61 0.031575378880063454 1105 62-63 0.2985285190333151 1105 64-65 0.08240031834551687 1105 66-67 0.20683421776148592 1105 68-69 0.28342477979208525 1105 70-71 0.2636991194459739 1105 72-73 0.3050687763227877 1105 74-75 0.3298006021482678 1105 76-77 0.21146183046397837 1105 78-79 0.18936712891117224 1105 80-81 0.09216348190297907 1105 82-83 0.17947358577388428 1105 84-85 0.12518462939194563 1105 86-87 0.25579870374153657 1105 88-89 0.24372752312898172 1105 90-91 -0.026675109074741243 1105 92-93 0.1291332572022874 1105 94-95 0.0773141006567144 1105 96-97 0.12486241393753872 1105 98-99 -0.030571571776540907 1105 100-101 0.18683746533848122 1105 102-103 -0.10060824381846345 1105 104-105 -0.09334693936462202 1105 106-107 -0.1304271009679745 1105 108-109 -0.1082943932074123 1105 110-111 -0.12309379132671694 1105 112-113 -0.1622628398506798 1105 114-115 -0.1327753748646998 1105 116-117 -0.38884233307628335 1105 118-119 -0.1903037128125007 1105 120-121 -0.17408967591911306 1105 122-123 -0.2714659470791574 1105 124-125 0.07499770799009653 1106 1 -0.16293913594304854 1106 2 -0.39258837781758515 1106 3 -0.0449535012862583 1106 4 -0.11714291642456232 1106 5 0.1424726495181261 1106 6 0.3534974157072952 1106 7 0.5104262897204066 1106 8 0.4345829566961541 1106 9 0.4330208659705548 1106 10-11 0.40774145382290783 1106 12-13 0.4934252919420601 1106 14-15 0.37931736616485523 1106 16-17 0.397266496383061 1106 18-19 0.5158300866310626 1106 20-21 0.5193991643301459 1106 22-23 0.5045497809940542 1106 24-25 0.5895994685539137 1106 26-27 0.6348353781604246 1106 28-29 0.719569276076264 1106 30-31 0.661879949270066 1106 32-33 0.3624338901791617 1106 34-35 0.4490097825192265 1106 36-37 0.5063400439608117 1106 38-39 0.44239264462901673 1106 40-41 0.4085951264273433 1106 42-43 0.37128433432285846 1106 44-45 0.3728630246095861 1106 46-47 0.33329381489776466 1106 48-49 0.5018184974041304 1106 50-51 0.46094604622528834 1106 52-53 0.26871875544224366 1106 54-55 0.2839015910334588 1106 56-57 -0.11460081635722119 1106 58-59 0.3451676681520759 1106 60-61 0.3171046676256246 1106 62-63 0.19399950030053859 1106 64-65 0.40700185843731873 1106 66-67 0.2764058144540833 1106 68-69 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-1.3595681900298615 2216 60-61 -1.1909847990372597 2216 62-63 -1.3186625881788032 2216 64-65 -1.1219872058949498 2216 66-67 -1.0315688357676152 2216 68-69 -0.9815100402897947 2216 70-71 -1.1168146245131467 2216 72-73 -0.9716149741004578 2216 74-75 -0.9154205632409642 2216 76-77 -0.8881530154743977 2216 78-79 -0.9829325601952412 2216 80-81 -1.0039134103924 2216 82-83 -0.8656213752874642 2216 84-85 -0.8941384844689857 2216 86-87 -1.0191093363975945 2216 88-89 -0.9553309464126833 2216 90-91 -1.0157534327118896 2216 92-93 -0.9781461930393469 2216 94-95 -1.0386765263633215 2216 96-97 -1.0186546082559538 2216 98-99 -1.1615970588944649 2216 100-101 -1.1873391283759283 2216 102-103 -0.9819011455945592 2216 104-105 -0.9315302896278013 2216 106-107 -1.0368858383090647 2216 108-109 -1.0014054837974946 2216 110-111 -0.9871932772749403 2216 112-113 -1.0302792771948468 2216 114-115 -1.11251342393048 2216 116-117 -1.1345867847030888 2216 118-119 -1.1553166410607254 2216 120-121 -1.2193077631540064 2216 122-123 -1.207240256803047 2216 124-125 -1.5262023874326864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41528.0 3 15048.0 4 7581.0 5 6677.0 6 7825.0 7 9199.0 8 9971.0 9 10508.0 10 11571.0 11 12524.0 12 13776.0 13 15772.0 14 18562.0 15 15721.0 16 10228.0 17 8863.0 18 8278.0 19 7986.0 20 7953.0 21 8281.0 22 9155.0 23 11761.0 24 14525.0 25 18464.0 26 22939.0 27 25519.0 28 29387.0 29 34204.0 30 39826.0 31 46548.0 32 57020.0 33 70585.0 34 92086.0 35 132798.0 36 203135.0 37 281736.0 38 93959.0 39 236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68152017434629 18.92995454663043 14.128859358842503 23.259665920180776 2 15.665200379400085 20.881183236550925 38.771936686563286 24.681679697485706 3 18.874233010997145 26.06697468456104 29.514889277694543 25.543903026747266 4 12.792534932205024 17.111292264367542 36.06529067873278 34.03088212469466 5 13.090620820193683 38.689911191666056 33.212710452702495 15.006757535437773 6 31.331636091874543 37.00831508624152 16.689471166102663 14.970577655781273 7 27.452905965593594 33.752366051784236 20.61499884753197 18.179729135090206 8 26.596366628413055 34.67998628178362 19.146271529881474 19.577375559921855 9 26.99056292096141 15.573939385049728 19.480642847314332 37.95485484667453 10-11 25.040501743172676 26.803999501302094 26.33844335710297 21.817055398422255 12-13 26.41808714601515 24.253196296800734 27.541654007201053 21.787062549983062 14-15 23.261462491312987 25.06699913042567 26.283111050578494 25.388427327682844 16-17 21.92092447436934 28.287651499145266 26.766138227636798 23.025285798848596 18-19 21.89780919932333 25.70181614484457 30.682769044655565 21.717605611176534 20-21 22.77256318193835 24.870426693515522 30.892141157636782 21.464868966909346 22-23 22.367966138985217 24.74120560019836 30.888956406038826 22.001871854777594 24-25 22.101017297195362 25.033438450551255 30.939210119191053 21.926334133062333 26-27 21.986436037395187 25.126018432181933 30.866605901231726 22.020939629191155 28-29 21.76984567674884 25.102201175496862 31.256587287451936 21.87136586030236 30-31 21.83965608160728 25.040667441949804 31.01150003317653 22.108176443266387 32-33 21.873007225499133 25.189158608262 30.6178517672614 22.319982398977466 34-35 22.154518818077367 25.241996598532552 30.5431521894764 22.06033239391368 36-37 22.4067652184238 24.983778422682967 30.452667567671394 22.15678879122184 38-39 22.180501279915628 24.938623418439864 30.59934275546802 22.281532546176493 40-41 21.95535486664781 25.049747334527687 30.647466186130814 22.34743161269369 42-43 22.33884063740846 25.081596803877815 30.169968604525277 22.409593954188452 44-45 22.259147118705627 25.237282038924803 29.82405961997157 22.679511222398 46-47 22.440011594324368 25.45935525778164 29.24444816952858 22.85618497836541 48-49 22.720056435024638 25.683244455154064 28.64737538720503 22.949323722616267 50-51 22.669232786688305 25.921968019886382 28.426833625112497 22.981965568312816 52-53 22.821506773250636 25.6882383960719 28.49203937879566 22.998215451881805 54-55 22.61766318487709 25.633235200648162 28.501189116701063 23.24791249777368 56-57 22.601872589980747 26.071985993817638 28.16170253486615 23.164438881335464 58-59 22.677845718330783 25.52420139195677 28.321979317962835 23.475973571749613 60-61 22.885589861252257 25.529025971985035 28.557589218675243 23.02779494808746 62-63 22.732907975288725 25.75109919084188 28.399634010483783 23.116358823385614 64-65 22.70294904949074 25.550268643060072 28.487444407387553 23.259337900061638 66-67 22.692327839998324 25.709820602276256 28.544982137057485 23.052869420667932 68-69 22.640362916321806 25.571142704480927 28.64810876314402 23.14038561605325 70-71 22.618536251471117 25.815252124171025 28.37064819956207 23.19556342479579 72-73 22.514327022807993 25.695572155461733 28.27705546068232 23.51304536104796 74-75 22.501324461912247 25.98107093353524 28.118614513308703 23.398990091243814 76-77 22.67067578846644 25.755708982458348 28.30715879684439 23.266456432230825 78-79 22.578899028102267 25.700216869741954 28.410425113585962 23.310458988569813 80-81 22.33329573325222 26.187047947786407 28.28153543830927 23.198120880652105 82-83 22.4891827049 25.937481447334875 28.106877320174473 23.46645852759065 84-85 22.307619776006035 25.815007665524696 27.978920680153802 23.89845187831547 86-87 22.600306620987823 25.601490499289326 28.068671926019828 23.729530953703023 88-89 22.40009303498346 25.51851113294189 28.183243994371804 23.898151837702848 90-91 22.289005996221366 25.842631492559725 28.18440563372412 23.68395687749479 92-93 22.285208792214284 25.73583055370946 28.131830828907063 23.847129825169198 94-95 22.237250608527418 25.68055541004446 28.14930835664421 23.932885624783918 96-97 22.39590009090963 25.717017842804918 27.991958880767285 23.895123185518163 98-99 22.217336793101882 25.658921767555242 27.95536060403927 24.16838083530361 100-101 22.11621471357368 25.6100738137279 28.126362005766524 24.147349466931896 102-103 22.303708437664792 25.16614457284344 28.33080144021065 24.19934554928112 104-105 22.169710176813446 25.51980638875211 28.142603205202082 24.167880229232363 106-107 22.152837462601553 25.650611628538034 27.999934344268624 24.196616564591793 108-109 22.181409269173415 25.599255448808613 27.780944099292114 24.438391182725855 110-111 21.91370646480358 25.60099516004944 28.08962866239747 24.39566971274951 112-113 22.104300027588906 25.55071294618068 27.88634069852312 24.458646327707292 114-115 21.890433634715922 25.72256737454906 27.786671416148938 24.60032757458608 116-117 21.75465431801276 25.733009251013627 27.93893423014734 24.57340220082627 118-119 21.687113886298793 25.82950057098555 27.715848254041425 24.76753728867423 120-121 21.655225930101153 25.663360168076853 27.824527877540817 24.856886024281184 122-123 21.66893323303166 25.671186528283883 27.6486521788024 25.011228059882058 124-125 21.620837603085686 25.593280050552004 27.53350677964443 25.252375566717884 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2258.0 1 1642.5 2 1912.5 3 2670.0 4 1715.0 5 1072.0 6 2038.5 7 4711.5 8 7613.0 9 8061.0 10 5356.5 11 2500.5 12 1653.0 13 1488.5 14 1529.0 15 1636.5 16 1733.5 17 1877.0 18 2059.0 19 2270.0 20 2459.5 21 2727.0 22 3176.5 23 3720.5 24 4355.5 25 5421.5 26 6853.5 27 8642.5 28 10402.5 29 11696.0 30 13584.5 31 16265.0 32 18775.5 33 20634.5 34 22402.0 35 23928.5 36 26646.0 37 30096.5 38 32209.5 39 33148.5 40 33846.0 41 36500.0 42 39699.0 43 43072.5 44 45944.0 45 47558.5 46 48879.5 47 49896.5 48 54386.0 49 55552.5 50 53127.0 51 52663.5 52 52931.0 53 53977.5 54 52006.0 55 47673.0 56 43398.5 57 40016.0 58 37630.0 59 35007.5 60 31151.5 61 26419.0 62 21742.5 63 16616.5 64 12291.0 65 9870.0 66 8180.0 67 6935.5 68 5501.5 69 4006.0 70 2977.5 71 2349.5 72 1967.5 73 1707.0 74 1340.5 75 1037.0 76 730.0 77 456.0 78 297.5 79 187.0 80 121.0 81 86.0 82 72.0 83 46.0 84 27.5 85 13.5 86 9.0 87 4.5 88 2.5 89 4.0 90 3.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10323139407781468 2 6.984532752220209E-5 3 0.0 4 0.09729454123842751 5 0.0 6 0.0 7 0.0017461331880550522 8 0.004330410306376529 9 0.0029335037559324877 10-11 4.539946288943135E-4 12-13 0.0 14-15 0.0 16-17 1.7461331880550522E-4 18-19 0.0014667518779662438 20-21 0.0011524479041163343 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.0476799128330312E-4 52-53 0.0 54-55 0.0 56-57 0.005692394193059469 58-59 6.635306114609197E-4 60-61 0.0 62-63 0.0 64-65 1.7461331880550522E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0015016745417273448 76-77 0.0 78-79 0.0 80-81 3.4922663761101044E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0010826025765941322 90-91 0.0 92-93 0.002549354454560376 94-95 0.0 96-97 0.006530538123325895 98-99 0.0027938131008880835 100-101 0.001606442533010648 102-103 0.0 104-105 0.0 106-107 0.001711210524293951 108-109 0.0 110-111 3.8414930137211143E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 6.286079476998187E-4 122-123 0.0037716476861989127 124-125 0.07885537477256616 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1431735.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.09082491799606 #Duplication Level Percentage of deduplicated Percentage of total 1 69.3511625109677 28.496964765976664 2 12.278939247125935 10.091034855647242 3 5.754787698553507 7.094069212844987 4 3.318806513860631 5.454899895910081 5 2.1175282940896194 4.350549219566972 6 1.4652114495663384 3.612404828518417 7 1.0888148091317027 3.1318209083106465 8 0.7898834842835764 2.5965571166650503 9 0.5925537275911619 2.1913669327458933 >10 3.0418837967593078 21.660695814375348 >50 0.14099432514216975 3.937413564822122 >100 0.056018403598776724 4.020637362069694 >500 0.0020494435977092833 0.574264719546583 >1k 8.539348323788681E-4 0.8151850155509115 >5k 3.415739329515472E-4 1.1459328614782054 >10k+ 1.707869664757736E-4 0.8262029259711501 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11773 0.8222890409188851 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9270 0.6474661861308133 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7059 0.49303816697922453 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2711 0.18935068291268986 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2626 0.18341383007330267 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2152 0.1503071448277789 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2091 0.14604657984892455 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2036 0.14220508683520344 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.984532752220209E-5 0.0 14-15 0.0 0.0 0.0 6.984532752220209E-5 0.0 16-17 0.0 0.0 3.4922663761101044E-5 2.0953598256660626E-4 0.0 18-19 0.0 0.0 6.984532752220209E-5 4.5399462889431357E-4 0.0 20-21 0.0 0.0 6.984532752220209E-5 7.68298602744223E-4 0.0 22-23 0.0 0.0 6.984532752220209E-5 0.001711210524293951 0.0 24-25 0.0 0.0 6.984532752220209E-5 0.002758890437126982 0.0 26-27 0.0 0.0 6.984532752220209E-5 0.0042954876426154285 0.0 28-29 0.0 0.0 6.984532752220209E-5 0.006111466158192682 0.0 30-31 0.0 0.0 6.984532752220209E-5 0.012327700307668669 0.0 32-33 0.0 0.0 6.984532752220209E-5 0.021023443584182826 0.0 34-35 0.0 0.0 6.984532752220209E-5 0.031465320048752035 0.0 36-37 0.0 0.0 6.984532752220209E-5 0.04732020939629191 0.0 38-39 0.0 0.0 6.984532752220209E-5 0.07658540162809459 0.0 40-41 0.0 0.0 6.984532752220209E-5 0.11011115883875158 0.0 42-43 0.0 0.0 6.984532752220209E-5 0.14133202024117592 0.0 44-45 0.0 0.0 6.984532752220209E-5 0.17527684941696614 0.0 46-47 0.0 0.0 6.984532752220209E-5 0.22004770435869767 0.0 48-49 0.0 0.0 6.984532752220209E-5 0.2682409803490171 0.0 50-51 0.0 0.0 6.984532752220209E-5 0.32156788791221835 0.0 52-53 0.0 0.0 6.984532752220209E-5 0.370878689142893 0.0 54-55 0.0 0.0 6.984532752220209E-5 0.42486912731755533 0.0 56-57 0.0 0.0 6.984532752220209E-5 0.4854948716068267 0.0 58-59 0.0 0.0 6.984532752220209E-5 0.5584133935400057 0.0 60-61 0.0 0.0 6.984532752220209E-5 0.6472217274844856 0.0 62-63 0.0 0.0 6.984532752220209E-5 0.7308265845285615 0.0 64-65 0.0 0.0 6.984532752220209E-5 0.8166315693895867 0.0 66-67 0.0 0.0 6.984532752220209E-5 0.9158119344711138 0.0 68-69 0.0 0.0 6.984532752220209E-5 1.0260627839649097 0.0 70-71 0.0 0.0 6.984532752220209E-5 1.1580704529818717 0.0 72-73 0.0 0.0 6.984532752220209E-5 1.296783273440965 0.0 74-75 0.0 0.0 6.984532752220209E-5 1.4377660670445298 0.0 76-77 0.0 0.0 6.984532752220209E-5 1.5860127747104038 0.0 78-79 0.0 0.0 6.984532752220209E-5 1.757517976441171 0.0 80-81 0.0 0.0 6.984532752220209E-5 1.9570311545083414 0.0 82-83 0.0 0.0 6.984532752220209E-5 2.1628653347162707 0.0 84-85 0.0 0.0 6.984532752220209E-5 2.376592036934209 0.0 86-87 0.0 0.0 6.984532752220209E-5 2.600376466315345 0.0 88-89 0.0 0.0 6.984532752220209E-5 2.846232019193496 0.0 90-91 0.0 0.0 6.984532752220209E-5 3.131096187492797 0.0 92-93 0.0 0.0 6.984532752220209E-5 3.4481939744435945 0.0 94-95 0.0 0.0 6.984532752220209E-5 3.7613105777256264 0.0 96-97 0.0 0.0 6.984532752220209E-5 4.0698173893911935 0.0 98-99 0.0 0.0 6.984532752220209E-5 4.4125134888788775 0.0 100-101 0.0 0.0 6.984532752220209E-5 4.808257114619675 0.0 102-103 0.0 0.0 6.984532752220209E-5 5.239342476086706 0.0 104-105 0.0 0.0 6.984532752220209E-5 5.671335826811526 0.0 106-107 0.0 0.0 6.984532752220209E-5 6.103364100200107 0.0 108-109 0.0 0.0 6.984532752220209E-5 6.563714653898941 0.0 110-111 0.0 0.0 6.984532752220209E-5 7.084935410533374 0.0 112-113 0.0 0.0 6.984532752220209E-5 7.6482030543361725 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5370 0.0 54.96155 1 GTATCAA 9030 0.0 48.862087 1 ATCAACG 11415 0.0 38.14557 3 TCAACGC 11510 0.0 37.727226 4 TATCAAC 11640 0.0 37.357044 2 CAACGCA 11750 0.0 36.96467 5 AACGCAG 11870 0.0 36.540848 6 ACGCAGA 13740 0.0 31.394463 7 GTACATG 7285 0.0 31.347094 1 CGCAGAG 13690 0.0 31.291822 8 TACATGG 7665 0.0 29.297585 2 GCAGAGT 14315 0.0 28.968636 9 GAGTACT 9325 0.0 27.755062 12-13 ACATGGG 7965 0.0 26.922747 3 AGTACTT 9740 0.0 26.022705 12-13 CAGAGTA 13820 0.0 24.989965 10-11 GTACTTT 10410 0.0 24.947975 14-15 TATCACG 120 0.0012796491 24.819407 2 AGAGTAC 13665 0.0 24.576824 10-11 TACTTTT 10760 0.0 23.77705 14-15 >>END_MODULE