FastQCFastQC Report
Thu 26 May 2016
SRR1364524_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364524_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435929
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.1441575453939575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14920.10390485880569303No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38950.054.6777531
GGTATCA21300.052.7858241
ATCAACG47900.044.2129633
TCAACGC48400.043.879134
CAACGCA49400.042.7500425
TATCAAC51050.041.4848332
AACGCAG51500.040.4292766
GTACATG75550.037.8743971
TACATGG74150.037.7069852
ACATGGG75200.036.705853
ACGCAGA60850.033.9249427
CGCAGAG61450.033.0162778
CATGGGA42950.029.779024
CATGGGG35750.028.9539814
GCAGAGT68900.028.4100699
TAAACCG900.00953849826.439465
ATGGGAG21850.024.5033915
TATAACG1501.6890377E-423.7955152
GAGTACT28000.023.69918812-13
GTGGTAT13850.023.1941851
Produced by FastQC (version 0.11.5)