FastQCFastQC Report
Thu 26 May 2016
SRR1364524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435929
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46070.320837590159402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37330.25997107099306443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27970.19478678959753581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.065.341281
GTATCAA54300.056.8738371
ATCAACG70650.043.411833
TCAACGC71700.042.7760934
CAACGCA73900.041.7856755
TATCAAC73800.041.478192
AACGCAG75850.040.4760976
GTACATG70350.035.3390241
ACGCAGA86750.035.1159937
TACATGG70300.034.394092
CGCAGAG88200.034.336328
ACATGGG70650.033.296453
GCAGAGT95150.031.01549
CATGGGA43350.026.9265064
GAGTACT58600.026.44932712-13
CAGAGTA92450.025.70938710-11
AGAGTAC90400.025.66717110-11
CATGGGG35900.025.0492974
AGTACTT62000.024.75896612-13
GTACTTT63600.024.2296514-15
Produced by FastQC (version 0.11.5)