##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364524_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1435929 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.094411353207576 31.0 30.0 34.0 26.0 34.0 2 29.996600806864407 31.0 30.0 34.0 26.0 34.0 3 30.245741955208093 31.0 30.0 34.0 26.0 34.0 4 33.82361035956513 35.0 35.0 37.0 29.0 37.0 5 33.228193733812745 35.0 33.0 37.0 27.0 37.0 6 33.25791525904136 35.0 33.0 37.0 27.0 37.0 7 33.04429675840519 35.0 33.0 37.0 26.0 37.0 8 32.97913058375449 35.0 33.0 37.0 26.0 37.0 9 34.223797973298126 38.0 34.0 39.0 25.0 39.0 10-11 34.14907039275619 38.0 34.0 39.0 25.0 39.0 12-13 34.01975968171128 38.0 34.0 39.0 25.0 39.0 14-15 34.986306774220736 39.0 34.0 41.0 25.0 41.0 16-17 34.99026414258644 39.0 34.0 41.0 25.0 41.0 18-19 35.003365068885714 39.0 34.0 41.0 25.0 41.0 20-21 35.01580022410579 39.0 34.0 41.0 25.0 41.0 22-23 34.96714322226238 39.0 34.0 41.0 25.0 41.0 24-25 34.91890650582306 39.0 34.0 41.0 24.0 41.0 26-27 34.79237448369662 39.0 34.0 41.0 24.0 41.0 28-29 34.633949867994865 39.0 33.5 41.0 23.5 41.0 30-31 34.46996265135672 38.5 33.0 41.0 23.0 41.0 32-33 34.26212681824798 38.0 33.0 40.0 22.5 41.0 34-35 34.07894575567455 38.0 33.0 40.0 21.0 41.0 36-37 33.87093373001033 38.0 33.0 40.0 18.5 41.0 38-39 33.69411126873265 38.0 33.0 40.0 18.0 41.0 40-41 33.71701142605241 38.0 33.0 40.0 17.5 41.0 42-43 33.57805190925178 38.0 33.0 40.0 15.5 41.0 44-45 33.410362211502104 38.0 32.5 40.0 15.0 41.0 46-47 33.15953609126913 38.0 32.0 40.0 10.5 41.0 48-49 32.870665959110795 38.0 32.0 40.0 8.0 41.0 50-51 32.69760238841893 38.0 31.5 40.0 7.0 41.0 52-53 32.50645366170612 37.5 31.0 40.0 3.5 41.0 54-55 32.302624642304735 37.0 31.0 40.0 2.0 41.0 56-57 32.072878951535905 37.0 31.0 40.0 2.0 41.0 58-59 31.859884437183176 36.5 31.0 40.0 2.0 41.0 60-61 31.602152334829924 36.0 30.0 40.0 2.0 41.0 62-63 31.420412151297175 36.0 30.0 39.5 2.0 41.0 64-65 31.27842323680349 35.5 30.0 39.0 2.0 41.0 66-67 30.972025427441054 35.0 30.0 39.0 2.0 41.0 68-69 30.68247524773161 35.0 30.0 38.5 2.0 40.5 70-71 30.365452609425674 35.0 29.5 37.5 2.0 40.0 72-73 30.082701860607315 35.0 29.0 37.0 2.0 39.5 74-75 29.735462547242935 35.0 29.0 36.5 2.0 39.0 76-77 29.43687536082912 35.0 29.0 36.0 2.0 39.0 78-79 29.16723981478193 34.0 29.0 36.0 2.0 38.5 80-81 28.899134288673046 34.0 29.0 35.0 2.0 37.0 82-83 28.624600868148775 34.0 29.0 35.0 2.0 37.0 84-85 28.417318683583936 34.0 29.0 35.0 2.0 36.0 86-87 28.21652393676846 34.0 28.0 35.0 2.0 36.0 88-89 28.051411664504304 34.0 28.0 35.0 2.0 36.0 90-91 27.920218548410123 34.0 28.0 35.0 2.0 35.5 92-93 27.812060345602045 34.0 27.0 35.0 2.0 35.0 94-95 27.708499863154792 34.0 27.0 35.0 2.0 35.0 96-97 27.598894861793305 34.0 27.0 35.0 2.0 35.0 98-99 27.515337109286044 34.0 27.0 35.0 2.0 35.0 100-101 27.41548084898348 34.0 27.0 35.0 2.0 35.0 102-103 26.139359954426716 31.5 24.5 34.0 2.0 35.0 104-105 27.08166141919273 33.5 25.5 35.0 2.0 35.0 106-107 27.250015495195097 34.0 27.0 35.0 2.0 35.0 108-109 27.251470998914293 34.0 27.0 35.0 2.0 35.0 110-111 27.170817986126053 34.0 27.0 35.0 2.0 35.0 112-113 27.091731554972426 34.0 26.0 35.0 2.0 35.0 114-115 26.987452374037993 34.0 25.5 35.0 2.0 35.0 116-117 26.867258060809412 34.0 25.0 35.0 2.0 35.0 118-119 26.72793640911215 34.0 25.0 35.0 2.0 35.0 120-121 26.535144495305826 34.0 25.0 35.0 2.0 35.0 122-123 26.24356357452214 34.0 25.0 35.0 2.0 35.0 124-125 24.448489096605755 31.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7136910281291904 1101 2 0.8057582359062607 1101 3 0.6224916112163008 1101 4 0.6731390949218934 1101 5 0.6140306513776608 1101 6 0.7642536617759959 1101 7 0.738671719701415 1101 8 0.7915583074162384 1101 9 0.9309453003894106 1101 10-11 0.9083191480834785 1101 12-13 0.8126924863120664 1101 14-15 1.009248039390684 1101 16-17 0.9802321850001263 1101 18-19 1.0716855056166068 1101 20-21 1.0422725038841563 1101 22-23 1.085478210379371 1101 24-25 1.0016404412862485 1101 26-27 1.1103585801236235 1101 28-29 1.16940352844572 1101 30-31 1.095241519871152 1101 32-33 1.2265527789758295 1101 34-35 1.275003221770028 1101 36-37 1.2925542108737957 1101 38-39 1.3383429543002592 1101 40-41 1.0254189950926644 1101 42-43 1.1316696613284662 1101 44-45 0.9874909242730752 1101 46-47 1.1201493995478273 1101 48-49 1.2445670173747132 1101 50-51 1.2868843218586221 1101 52-53 1.3088304261244303 1101 54-55 1.3009724757494112 1101 56-57 1.2692365895933762 1101 58-59 1.3112224653924187 1101 60-61 1.2958602418964311 1101 62-63 1.3355115466374734 1101 64-65 1.2134898741433595 1101 66-67 1.3780211262150246 1101 68-69 1.3771996709080199 1101 70-71 1.34134156297484 1101 72-73 1.2523669332413405 1101 74-75 1.3811446903744198 1101 76-77 1.4665821924187945 1101 78-79 1.4917400641363727 1101 80-81 1.1856336031491068 1101 82-83 1.3428600441092726 1101 84-85 1.3680755996609868 1101 86-87 1.403186003062764 1101 88-89 1.2969859800737638 1101 90-91 1.2436416280992866 1101 92-93 1.1568736480231934 1101 94-95 1.1927951731074593 1101 96-97 1.2233192333785823 1101 98-99 1.211592704001017 1101 100-101 1.2109848970190278 1101 102-103 1.1927173391422805 1101 104-105 1.1870243265022502 1101 106-107 1.1473774572738797 1101 108-109 1.1931427632459126 1101 110-111 1.1942651233372779 1101 112-113 1.2790762441946448 1101 114-115 1.3128985491710026 1101 116-117 1.3312114230275434 1101 118-119 1.3800037284943976 1101 120-121 1.4442203787778745 1101 122-123 1.2863608303037708 1101 124-125 1.6134680199331655 1102 1 0.2691367472029853 1102 2 -0.4455094875583647 1102 3 0.12757171292356517 1102 4 0.12326631669906618 1102 5 0.4327018009512713 1102 6 0.1775784274252885 1102 7 0.3965240635154714 1102 8 0.38521265266584237 1102 9 0.6897189552432863 1102 10-11 0.7884712135135743 1102 12-13 0.7519822893211057 1102 14-15 0.8941579318210913 1102 16-17 0.9258675112188612 1102 18-19 1.0714601325562256 1102 20-21 0.8748413665350654 1102 22-23 0.9288317659024798 1102 24-25 0.9516126642757925 1102 26-27 0.9990605716059306 1102 28-29 0.6200996637362053 1102 30-31 0.9380606913138436 1102 32-33 1.089963221465581 1102 34-35 1.0008781099056279 1102 36-37 0.832828574074334 1102 38-39 0.3242958329149417 1102 40-41 0.12732324398870531 1102 42-43 0.24427529398879244 1102 44-45 0.05385386347101928 1102 46-47 0.004494872772063729 1102 48-49 -0.24452365494294526 1102 50-51 -0.15738265226249837 1102 52-53 -0.4734880268868267 1102 54-55 -0.5059033099322079 1102 56-57 -0.4400881769328464 1102 58-59 -0.37139351075135707 1102 60-61 -0.33173346659973646 1102 62-63 -0.3312753701494451 1102 64-65 -0.33067294166940897 1102 66-67 -0.40402591449199576 1102 68-69 -0.3024038040464134 1102 70-71 -0.7223889180300276 1102 72-73 -0.5200222798953646 1102 74-75 -0.3560801867775325 1102 76-77 -0.8515012990922237 1102 78-79 -0.5509532228595546 1102 80-81 -0.5961379694155369 1102 82-83 -0.8085095357015106 1102 84-85 -0.6646587411863898 1102 86-87 -0.3445044711546039 1102 88-89 -0.5723626479348454 1102 90-91 -0.5419084509785996 1102 92-93 -0.42039565919142063 1102 94-95 -0.337220440773244 1102 96-97 -0.4928898251276479 1102 98-99 -0.6995753423083215 1102 100-101 -0.546845391804883 1102 102-103 -0.5811845920066894 1102 104-105 -0.5250183996699214 1102 106-107 -0.46452070567259796 1102 108-109 -0.40970386512487167 1102 110-111 -0.3480419820965359 1102 112-113 -0.2637924303969079 1102 114-115 -0.32528265076953744 1102 116-117 -0.49104223742119757 1102 118-119 -0.1009855715468646 1102 120-121 0.16714480908607854 1102 122-123 0.1873192358200626 1102 124-125 0.4535875378102041 1103 1 0.1811649333428491 1103 2 -0.1988661140114374 1103 3 0.07311122699099926 1103 4 0.13579430384634605 1103 5 0.15164613918494751 1103 6 0.2777632585745877 1103 7 0.3794302342389031 1103 8 0.44369449794971416 1103 9 0.6222445984288214 1103 10-11 0.704968797523847 1103 12-13 0.577731929791554 1103 14-15 0.6534672444298906 1103 16-17 0.6405353400998877 1103 18-19 0.7159897897762448 1103 20-21 0.6276864721720443 1103 22-23 0.670883660238637 1103 24-25 0.5318396379037793 1103 26-27 0.492983362483038 1103 28-29 0.5395723700109372 1103 30-31 0.5543553761364421 1103 32-33 0.6346156925139894 1103 34-35 0.6451684876985411 1103 36-37 0.5557600888840142 1103 38-39 0.3877781298997789 1103 40-41 0.1851055631923444 1103 42-43 0.38104424040241014 1103 44-45 0.4398640743543254 1103 46-47 0.45605543854583175 1103 48-49 0.43317428163824445 1103 50-51 0.4608433593645316 1103 52-53 0.4256202910032272 1103 54-55 0.3744386643183901 1103 56-57 0.3565059978339207 1103 58-59 0.5398985249061639 1103 60-61 0.4884016530277684 1103 62-63 0.4867575087971403 1103 64-65 0.33828275012892206 1103 66-67 0.4121330158801335 1103 68-69 0.5260462756727016 1103 70-71 0.3647625092229241 1103 72-73 0.4122372259681981 1103 74-75 0.41522850077481266 1103 76-77 0.4028295364568848 1103 78-79 0.22837254631392767 1103 80-81 0.34150654437110717 1103 82-83 0.5277928158292688 1103 84-85 0.5134921171675124 1103 86-87 0.7036309805341503 1103 88-89 0.6654310492497189 1103 90-91 0.6811317162240051 1103 92-93 0.5651084024547757 1103 94-95 0.4170408084118655 1103 96-97 0.4570165384842966 1103 98-99 0.4308575825408454 1103 100-101 0.45609921012449206 1103 102-103 0.24841671475197913 1103 104-105 0.37285689668253497 1103 106-107 0.498447140543977 1103 108-109 0.557071698097392 1103 110-111 0.5650629614652587 1103 112-113 0.5133016918748758 1103 114-115 0.4852043805296731 1103 116-117 0.4011355227768689 1103 118-119 0.2361066462560153 1103 120-121 0.2459214563906471 1103 122-123 0.26477618383538726 1103 124-125 0.5056308132037337 1104 1 0.3145749691117956 1104 2 0.4021271894900771 1104 3 0.27049088525450316 1104 4 -0.011948577339353506 1104 5 0.374828301901303 1104 6 0.4948150034853924 1104 7 0.444212540615716 1104 8 0.22615079948634076 1104 9 0.5074204258859325 1104 10-11 0.4263778602437469 1104 12-13 0.5981969678887253 1104 14-15 0.6063928646442562 1104 16-17 0.6669167669740048 1104 18-19 0.5732219872592594 1104 20-21 0.41705089992106537 1104 22-23 0.5498543163367984 1104 24-25 0.6908382535013899 1104 26-27 0.5737511315906048 1104 28-29 0.7470389553813774 1104 30-31 0.5930143433263169 1104 32-33 0.6467495723695791 1104 34-35 0.7794297325748971 1104 36-37 0.7862717539880961 1104 38-39 0.8893984862528868 1104 40-41 0.8528506577514179 1104 42-43 0.6936637051419083 1104 44-45 0.6459091937616463 1104 46-47 0.7898823611007231 1104 48-49 0.7382758984448472 1104 50-51 0.5597432501575241 1104 52-53 0.5529088877453887 1104 54-55 0.16834771137763482 1104 56-57 0.3575170500841409 1104 58-59 0.4563849199682508 1104 60-61 0.563795623046996 1104 62-63 0.5454662977686944 1104 64-65 0.5544598993457832 1104 66-67 0.5144091362959955 1104 68-69 0.29329972989241426 1104 70-71 -0.12762192032185382 1104 72-73 -0.14744910143983248 1104 74-75 0.2030945165148168 1104 76-77 0.2408227167612047 1104 78-79 0.37452837615145285 1104 80-81 0.17506838414375636 1104 82-83 0.2973599760503518 1104 84-85 0.3832275772881637 1104 86-87 0.35756796621195264 1104 88-89 0.29332874128595776 1104 90-91 0.31640589262986296 1104 92-93 0.3745238004339235 1104 94-95 0.2860154736391429 1104 96-97 0.37907165565735923 1104 98-99 0.40496706517525993 1104 100-101 0.32416399096920756 1104 102-103 0.3014703347658845 1104 104-105 0.3452749235645811 1104 106-107 0.3331778920260007 1104 108-109 0.34079411184369235 1104 110-111 0.32994800177580075 1104 112-113 0.38271182787205404 1104 114-115 0.3814641971537789 1104 116-117 0.35190174669925867 1104 118-119 0.384129748862577 1104 120-121 0.3229185560362957 1104 122-123 0.36218424009683403 1104 124-125 0.9280470652109081 1105 1 0.25468519543800383 1105 2 -0.014159742775785134 1105 3 0.3150326765039324 1105 4 0.3097078464241818 1105 5 -0.10622693200731703 1105 6 0.30541686869656104 1105 7 0.6475129861461824 1105 8 0.5887835428038954 1105 9 0.7798024483118482 1105 10-11 0.7863569651306648 1105 12-13 0.7754328575477842 1105 14-15 0.8867562677029994 1105 16-17 0.8072685866497906 1105 18-19 0.9008557231458525 1105 20-21 0.9874026582008355 1105 22-23 1.0046072894391926 1105 24-25 0.9637238256406917 1105 26-27 0.9697733856023234 1105 28-29 1.0686505600482903 1105 30-31 1.1151370698827634 1105 32-33 1.1094760469485578 1105 34-35 1.1024230547341602 1105 36-37 1.2868714908038115 1105 38-39 1.2975428733261651 1105 40-41 1.156075586173884 1105 42-43 1.0343329422553964 1105 44-45 1.1153537234783428 1105 46-47 1.1146475724770042 1105 48-49 1.0376369705012607 1105 50-51 0.9261965656450855 1105 52-53 1.0178288375643731 1105 54-55 1.0749657933249495 1105 56-57 1.09929642961594 1105 58-59 0.7977176434344244 1105 60-61 0.7005366402972406 1105 62-63 1.0423184213547962 1105 64-65 0.9292503854181042 1105 66-67 1.0094406631536863 1105 68-69 0.9470882738038746 1105 70-71 1.1321048469114992 1105 72-73 1.1047219642518655 1105 74-75 0.9629559456914372 1105 76-77 0.9390696556578355 1105 78-79 0.8736050822957644 1105 80-81 0.9834927370808444 1105 82-83 0.9660950747239951 1105 84-85 0.9613382579449343 1105 86-87 1.0843577114389227 1105 88-89 0.9363204876139619 1105 90-91 0.7796619079051936 1105 92-93 0.8719521256936815 1105 94-95 0.9122034355163038 1105 96-97 0.978806527165716 1105 98-99 0.9479697604606585 1105 100-101 0.9127074931211361 1105 102-103 0.5981998709598635 1105 104-105 0.7790492849947803 1105 106-107 0.7526330637199763 1105 108-109 0.9020347003450375 1105 110-111 0.8188723639222886 1105 112-113 0.6914063233093053 1105 114-115 0.7881221865158992 1105 116-117 0.6019129003100616 1105 118-119 0.8311574650737867 1105 120-121 0.6488560221405777 1105 122-123 0.8738971686666517 1105 124-125 1.3134548678267848 1106 1 -0.21349291464145637 1106 2 -0.5921137685737321 1106 3 -0.11550704562863245 1106 4 -0.2656233497887541 1106 5 -0.10942550976726295 1106 6 0.05147432025882637 1106 7 0.05327589948208811 1106 8 0.10645343395281515 1106 9 0.2503075754162012 1106 10-11 0.12932426175692058 1106 12-13 0.21293838053123437 1106 14-15 0.1651657111429543 1106 16-17 0.047376675222253084 1106 18-19 0.18365221726563163 1106 20-21 0.11051593916113944 1106 22-23 0.12853927761923245 1106 24-25 0.037604997150346264 1106 26-27 0.4059755367195024 1106 28-29 0.29704052289415017 1106 30-31 0.33476922612861415 1106 32-33 0.2058855593278679 1106 34-35 0.3368336179165681 1106 36-37 0.4513198766303006 1106 38-39 0.3546059726939035 1106 40-41 0.4332828732739742 1106 42-43 0.36415527714822815 1106 44-45 0.28984291932150796 1106 46-47 0.21089173482712908 1106 48-49 0.4607101412839256 1106 50-51 0.3931046245527199 1106 52-53 0.3581234802443163 1106 54-55 0.2833749338495011 1106 56-57 -0.33825171043125124 1106 58-59 0.072828651484663 1106 60-61 0.21111778872264964 1106 62-63 0.11715126950121757 1106 64-65 0.14185970915423596 1106 66-67 0.08384942973438925 1106 68-69 0.1456363689360245 1106 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-1.3212582446556205 2216 78-79 -1.2471059351413025 2216 80-81 -1.208033839375922 2216 82-83 -1.0412475265040158 2216 84-85 -1.0178622879383248 2216 86-87 -1.1555079837710167 2216 88-89 -1.1226397304699915 2216 90-91 -1.2535352351860283 2216 92-93 -1.3228220858113957 2216 94-95 -1.2336994098328553 2216 96-97 -1.2661431548325908 2216 98-99 -1.1839810202623866 2216 100-101 -1.2992462167863508 2216 102-103 -1.3181107482876833 2216 104-105 -1.2198104654297275 2216 106-107 -1.3415946472626707 2216 108-109 -1.334635265862726 2216 110-111 -1.410561899455164 2216 112-113 -1.3334196371208904 2216 114-115 -1.4272085026953931 2216 116-117 -1.3480033766403992 2216 118-119 -1.3914358619971523 2216 120-121 -1.5235539783869 2216 122-123 -1.4332519492672517 2216 124-125 -1.9090399780431184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43482.0 3 15452.0 4 7591.0 5 6036.0 6 6486.0 7 7668.0 8 8457.0 9 8894.0 10 10201.0 11 10814.0 12 11788.0 13 13328.0 14 15001.0 15 13292.0 16 9376.0 17 8327.0 18 7759.0 19 7373.0 20 7195.0 21 7687.0 22 8557.0 23 10592.0 24 13813.0 25 18528.0 26 22533.0 27 25458.0 28 29216.0 29 35113.0 30 40586.0 31 47162.0 32 57647.0 33 71410.0 34 92575.0 35 128151.0 36 206776.0 37 300163.0 38 101129.0 39 313.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32473772232342 18.91069278672776 13.514210643360222 24.250358847588593 2 15.809764967487947 20.10308309115562 39.15569641674484 24.931455524611593 3 19.654175101972314 24.700733810654985 28.694176383372717 26.950914703999988 4 12.967134998285232 16.349062732643894 35.74936776625614 34.93443450281473 5 13.400035795641706 38.463879481506396 33.5451822478688 14.590902474983094 6 32.726827022784555 36.28438453433282 16.75598166761727 14.232806775265349 7 28.9264682121825 32.317103745675176 20.174580888866604 18.581847153275724 8 26.951023907542922 35.425397141569675 18.755097250844983 18.868481700042413 9 26.78554888134413 15.666335857926352 19.12178984939497 38.42632541133455 10-11 25.634245385707686 26.613618177627014 26.423635316476762 21.328501120188534 12-13 26.364604378071615 23.63539562192838 27.392127326629662 22.60787267337034 14-15 23.080857814597948 24.81353521903605 25.429896206495034 26.67571075987097 16-17 22.811235135007145 28.316157401088084 25.887686256375687 22.984921207529087 18-19 22.777857286868915 26.338216352245936 28.626476388063328 22.257449972821817 20-21 23.39639092853693 25.551122824941263 28.764208329285378 22.288277917236428 22-23 23.06541618701203 25.430574910040814 28.773811239970783 22.730197662976373 24-25 22.812200324667863 25.656352089831742 28.842512408343307 22.688935177157088 26-27 22.552647101632463 26.012671935729415 28.69052021374316 22.744160748894966 28-29 22.523676309901116 26.062639587333358 28.831126051496973 22.58255805126855 30-31 22.648403925263715 25.688596023898118 28.780879834587918 22.882120216250247 32-33 22.457551870600845 25.852044216670876 28.721301679957712 22.96910223277056 34-35 22.96485411186765 25.819695820615085 28.56401674456049 22.651433322956777 36-37 22.90760894166773 25.676687357104704 28.401926557650135 23.013777143577432 38-39 22.69569038580598 25.854934331711387 28.58696356156885 22.862411720913776 40-41 22.862934030860856 25.689849567771105 28.560465036920352 22.886751364447687 42-43 22.75836757945553 25.764191683572097 28.33489678110826 23.142543955864113 44-45 22.820383180505445 25.839334674625274 28.266787564009082 23.073494580860196 46-47 23.136067312520325 26.182944978477344 27.618426816367663 23.062560892634664 48-49 23.22162168185196 26.270449304944744 27.36051016449978 23.14741884870352 50-51 23.101281470044828 26.440583065040123 27.307722039181602 23.150413425733447 52-53 23.39374021974624 26.30990111628082 27.18759075135331 23.108767912619633 54-55 23.100898442750303 26.29116759951223 27.34149808242608 23.266435875311384 56-57 23.07298266801364 26.589038200295512 27.126224958882194 23.211754172808654 58-59 23.18874643651433 26.237143681901166 27.16177833212679 23.412331549457715 60-61 23.119666780182026 26.24043389331924 27.511039891248107 23.12885943525063 62-63 22.983657269962514 26.339150473317275 27.51406928894117 23.16312296777905 64-65 22.86227444409508 26.149773194899872 27.53435156235582 23.453600798649234 66-67 22.81951266392698 26.500683529617415 27.31701915623962 23.362784650215993 68-69 22.867894490531558 26.23470675409909 27.503294037026926 23.394104718342422 70-71 22.78935796964892 26.231345700240055 27.484436904610188 23.494859425500845 72-73 22.98264747073149 26.389988641499684 27.23007196038244 23.397291927386384 74-75 22.935752981904866 26.54008500526843 27.05317390841076 23.470988104415945 76-77 23.044036299844908 26.37424970176102 27.179651640157697 23.402062358236375 78-79 23.004793412487665 26.428465474267878 27.119934202874934 23.446806910369524 80-81 22.93690621940128 26.633022871652162 27.104391173361304 23.32567973558526 82-83 23.031570502441276 26.625445965643145 27.006140275737867 23.33684325617771 84-85 22.956114125419848 26.541702270794726 26.904777325341296 23.59740627844413 86-87 22.973803022294277 26.46157992491272 27.076721759919884 23.487895292873116 88-89 22.934303590480727 26.54470202826365 26.988776439716194 23.53221794153943 90-91 22.930103090055287 26.682760777169346 26.979258723794842 23.407877408980525 92-93 22.89188972731293 26.484039571138556 27.105254910700292 23.518815790848215 94-95 22.79472035177227 26.33852370138078 27.106388964914004 23.76036698193295 96-97 22.960546958114858 26.324988481994826 26.967837689977436 23.74662686991288 98-99 22.738007436611557 26.281366893497943 26.979898584063033 24.000727085827467 100-101 22.628743745190285 26.275876718005147 27.16866483969928 23.92671469710529 102-103 22.809762878248158 26.034365208864784 27.11415397279392 24.04171794009314 104-105 22.719960388013615 26.13182824498983 27.152212957604448 23.995998409392108 106-107 22.62958351092131 26.215224095137014 27.108213061003912 24.046979332937767 108-109 22.73228690276469 26.17859239558502 26.77562748576009 24.3134932158902 110-111 22.590637599678814 26.18379308939989 27.124963742870882 24.10060556805041 112-113 22.54449906645802 26.191441220283174 27.176343677159526 24.087716036099277 114-115 22.498361687799324 26.312547486679357 26.902096134279617 24.2869946912417 116-117 22.363953928084186 26.191232296304346 27.125540329640252 24.319273445971216 118-119 22.343340095506115 26.28040801460239 26.833638710549057 24.542613179342432 120-121 22.21008903730374 26.312629925866087 27.08927756865831 24.388003468171863 122-123 22.239432254649667 26.341204369521993 26.97438111787054 24.4449822579578 124-125 22.116486380997518 26.224316261953216 26.920390866765175 24.73880649028409 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 243.0 1 202.5 2 436.5 3 660.5 4 420.0 5 266.0 6 551.0 7 1349.5 8 2142.5 9 2310.0 10 1738.0 11 1051.5 12 841.5 13 856.5 14 951.5 15 1062.0 16 1175.0 17 1318.0 18 1531.5 19 1754.0 20 2004.0 21 2328.5 22 2738.5 23 3271.5 24 3930.5 25 4936.5 26 6321.5 27 8105.0 28 9969.0 29 11994.5 30 14428.0 31 17115.0 32 20183.5 33 22655.0 34 25122.5 35 27240.5 36 29596.0 37 32626.0 38 34653.5 39 36172.0 40 37059.0 41 38789.0 42 40872.5 43 43117.0 44 46176.0 45 49057.5 46 50576.5 47 51068.5 48 52937.0 49 54583.0 50 54760.0 51 53780.5 52 53227.5 53 54063.5 54 54100.0 55 51126.5 56 46839.0 57 43155.0 58 40200.5 59 37008.0 60 31759.0 61 26013.5 62 21086.0 63 16301.5 64 12304.0 65 9491.5 66 7373.0 67 5905.5 68 4497.0 69 3248.5 70 2340.0 71 1737.5 72 1351.0 73 1111.0 74 874.5 75 652.5 76 473.0 77 291.5 78 177.5 79 109.0 80 74.0 81 44.0 82 26.5 83 21.5 84 17.5 85 10.0 86 4.5 87 1.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10279059758525665 2 0.0 3 0.0 4 0.09283188792760645 5 0.0 6 0.0 7 0.0022981637671500472 8 0.00738198058539106 9 0.0037606316189728044 10-11 6.964132627727416E-4 12-13 0.0 14-15 6.964132627727415E-5 16-17 3.482066313863708E-4 18-19 0.002402625756565958 20-21 0.001358005862406846 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.008043573185025165 58-59 7.312339259113787E-4 60-61 0.0 62-63 0.0 64-65 3.133859682477337E-4 66-67 0.0 68-69 6.964132627727415E-5 70-71 0.0 72-73 0.0 74-75 0.0012535438729909347 76-77 0.0 78-79 0.0 80-81 2.0892397883182245E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0014624678518227574 90-91 0.0 92-93 0.002367805093427321 94-95 0.0 96-97 0.0092971170580161 98-99 0.004178479576636449 100-101 0.002367805093427321 102-103 0.0 104-105 0.0 106-107 0.0014624678518227574 108-109 0.0 110-111 3.133859682477337E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.749785678818382E-4 122-123 0.005153458144518288 124-125 0.08141071041813348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1435929.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.31489023851011 #Duplication Level Percentage of deduplicated Percentage of total 1 80.88643688928695 49.59542999650805 2 10.994355951484396 13.482354568167523 3 3.3536710154905727 6.168899106326315 4 1.4676853648331016 3.599638681976372 5 0.8367383022946987 2.565225858177837 6 0.5151579077557306 1.8952110341725885 7 0.3430488234911845 1.4723800671168406 8 0.25092170535961744 1.2308189458067775 9 0.1824911382756978 1.0070481701587661 >10 1.072553154402962 12.019141828582427 >50 0.06837496048265583 2.8462021975694496 >100 0.027085379098366204 2.8472947569979663 >500 0.0010242053612291575 0.4149620963884078 >1k 4.5520238276851446E-4 0.8553926920506963 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4607 0.320837590159402 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3733 0.25997107099306443 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2797 0.19478678959753581 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.964132627727415E-5 0.0 0.0 0.0 0.0 8 1.392826525545483E-4 0.0 0.0 0.0 0.0 9 1.392826525545483E-4 0.0 0.0 0.0 6.964132627727415E-5 10-11 1.392826525545483E-4 0.0 0.0 6.964132627727415E-5 6.964132627727415E-5 12-13 1.392826525545483E-4 0.0 0.0 6.964132627727415E-5 6.964132627727415E-5 14-15 1.392826525545483E-4 0.0 0.0 6.964132627727415E-5 6.964132627727415E-5 16-17 1.741033156931854E-4 0.0 0.0 1.0446198941591124E-4 6.964132627727415E-5 18-19 2.0892397883182247E-4 0.0 0.0 1.392826525545483E-4 6.964132627727415E-5 20-21 2.0892397883182247E-4 0.0 0.0 2.0892397883182247E-4 6.964132627727415E-5 22-23 2.0892397883182247E-4 0.0 0.0 4.874892839409191E-4 1.392826525545483E-4 24-25 2.0892397883182247E-4 0.0 0.0 0.0010097992310204753 2.0892397883182247E-4 26-27 2.0892397883182247E-4 0.0 0.0 0.0011839025467136606 2.0892397883182247E-4 28-29 3.4820663138637075E-4 0.0 0.0 0.001810674483209128 2.0892397883182247E-4 30-31 3.4820663138637075E-4 0.0 0.0 0.003760631618972804 2.4374464197045954E-4 32-33 3.4820663138637075E-4 0.0 0.0 0.006929311964588779 3.4820663138637075E-4 34-35 3.4820663138637075E-4 0.0 0.0 0.010898867562393406 3.4820663138637075E-4 36-37 3.4820663138637075E-4 0.0 0.0 0.017305869579902627 3.4820663138637075E-4 38-39 4.1784795766364494E-4 0.0 0.0 0.028761867752514228 3.830272945250079E-4 40-41 4.1784795766364494E-4 0.0 0.0 0.043665111575850894 4.1784795766364494E-4 42-43 4.1784795766364494E-4 0.0 0.0 0.057663018157583 4.52668620802282E-4 44-45 4.1784795766364494E-4 0.0 0.0 0.07510817039004017 4.874892839409191E-4 46-47 4.52668620802282E-4 0.0 0.0 0.09718447081993609 4.874892839409191E-4 48-49 4.874892839409191E-4 0.0 0.0 0.12020092915457518 5.571306102181932E-4 50-51 4.874892839409191E-4 0.0 0.0 0.15355912444138953 5.571306102181932E-4 52-53 4.874892839409191E-4 0.0 0.0 0.18423612866652878 5.571306102181932E-4 54-55 4.874892839409191E-4 6.964132627727415E-5 0.0 0.2162014974277976 5.571306102181932E-4 56-57 4.874892839409191E-4 6.964132627727415E-5 0.0 0.2550613574905166 5.571306102181932E-4 58-59 4.874892839409191E-4 6.964132627727415E-5 0.0 0.3029397693061426 5.571306102181932E-4 60-61 4.874892839409191E-4 6.964132627727415E-5 0.0 0.3562850252345346 5.571306102181932E-4 62-63 4.874892839409191E-4 6.964132627727415E-5 0.0 0.4126944995191266 5.571306102181932E-4 64-65 5.571306102181932E-4 6.964132627727415E-5 0.0 0.47056644165554146 5.571306102181932E-4 66-67 6.267719364954674E-4 6.964132627727415E-5 0.0 0.5365167776401201 5.571306102181932E-4 68-69 6.267719364954674E-4 6.964132627727415E-5 0.0 0.6144106010812512 5.571306102181932E-4 70-71 6.267719364954674E-4 6.964132627727415E-5 0.0 0.7059193038095894 5.571306102181932E-4 72-73 6.615925996341044E-4 6.964132627727415E-5 0.0 0.7980547784744232 0.0010446198941591123 74-75 7.312339259113786E-4 6.964132627727415E-5 0.0 0.8913393350228318 0.0010446198941591123 76-77 8.356959153272899E-4 6.964132627727415E-5 0.0 0.9964280963752385 0.0010446198941591123 78-79 8.356959153272899E-4 6.964132627727415E-5 0.0 1.120911967095866 0.0010446198941591123 80-81 9.401579047432011E-4 6.964132627727415E-5 0.0 1.2647909471847147 0.0010794405572977493 82-83 9.749785678818382E-4 6.964132627727415E-5 0.0 1.4147983639859631 0.0011839025467136606 84-85 9.749785678818382E-4 6.964132627727415E-5 0.0 1.5591300126956138 0.0011839025467136606 86-87 9.749785678818382E-4 6.964132627727415E-5 0.0 1.7186086498705717 0.0011839025467136606 88-89 0.0010446198941591123 6.964132627727415E-5 0.0 1.9077196713765097 0.0011839025467136606 90-91 0.0010446198941591123 6.964132627727415E-5 0.0 2.1245479407408023 0.0011839025467136606 92-93 0.0010446198941591123 6.964132627727415E-5 0.0 2.3584731557061667 0.0012187232098522975 94-95 0.0010446198941591123 6.964132627727415E-5 0.0 2.588393994410587 0.0012535438729909347 96-97 0.0010446198941591123 6.964132627727415E-5 0.0 2.8202647902507714 0.0012535438729909347 98-99 0.0010446198941591123 6.964132627727415E-5 0.0 3.0756743543726746 0.0012535438729909347 100-101 0.0010446198941591123 6.964132627727415E-5 0.0 3.3821310106558196 0.0012535438729909347 102-103 0.0010446198941591123 6.964132627727415E-5 0.0 3.721702117583808 0.0012535438729909347 104-105 0.0010446198941591123 6.964132627727415E-5 0.0 4.060298245943915 0.0012535438729909347 106-107 0.0010446198941591123 6.964132627727415E-5 0.0 4.3887615613306785 0.0012535438729909347 108-109 0.0010446198941591123 6.964132627727415E-5 0.0 4.738186915926901 0.0012535438729909347 110-111 0.0010446198941591123 6.964132627727415E-5 0.0 5.137336177485098 0.001358005862406846 112-113 0.0010794405572977493 6.964132627727415E-5 0.0 5.5896217709928555 0.0014624678518227574 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2865 0.0 65.34128 1 GTATCAA 5430 0.0 56.873837 1 ATCAACG 7065 0.0 43.41183 3 TCAACGC 7170 0.0 42.776093 4 CAACGCA 7390 0.0 41.785675 5 TATCAAC 7380 0.0 41.47819 2 AACGCAG 7585 0.0 40.476097 6 GTACATG 7035 0.0 35.339024 1 ACGCAGA 8675 0.0 35.115993 7 TACATGG 7030 0.0 34.39409 2 CGCAGAG 8820 0.0 34.33632 8 ACATGGG 7065 0.0 33.29645 3 GCAGAGT 9515 0.0 31.0154 9 CATGGGA 4335 0.0 26.926506 4 GAGTACT 5860 0.0 26.449327 12-13 CAGAGTA 9245 0.0 25.709387 10-11 AGAGTAC 9040 0.0 25.667171 10-11 CATGGGG 3590 0.0 25.049297 4 AGTACTT 6200 0.0 24.758966 12-13 GTACTTT 6360 0.0 24.22965 14-15 >>END_MODULE