FastQCFastQC Report
Thu 26 May 2016
SRR1364523_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364523_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571267
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44670.1737275825497702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32600.12678574414870178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35250.057.5547521
GTATCAA68600.052.64421
ATCAACG83600.042.699473
TCAACGC85450.042.0535244
CAACGCA86250.041.732445
AACGCAG90800.039.834696
TATCAAC90200.039.772992
ACGCAGA104450.034.6289147
GTACATG113700.034.4834631
CGCAGAG105000.033.940918
TACATGG116300.032.2795262
ACATGGG119000.030.9971833
GCAGAGT113600.028.6480439
CATGGGG58950.026.5429654
GAGTACT53150.024.52091212-13
CAGAGTA110500.023.66691610-11
AGAGTAC106800.023.48396510-11
GAGTACA87850.022.6876221
CATGGGA64550.022.489054
AGTACAT85800.021.842422
Produced by FastQC (version 0.11.5)