##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364523_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2571267 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.842363706297323 31.0 26.0 31.0 2.0 34.0 2 25.906756474531818 31.0 26.0 31.0 2.0 34.0 3 26.067961825823613 31.0 26.0 31.0 2.0 34.0 4 29.155351816828045 35.0 28.0 37.0 2.0 37.0 5 29.2111849916792 35.0 28.0 37.0 2.0 37.0 6 29.19442282734543 35.0 29.0 37.0 2.0 37.0 7 28.904562225548727 35.0 28.0 37.0 2.0 37.0 8 28.809641705820514 35.0 28.0 37.0 2.0 37.0 9 29.6774555112324 35.0 27.0 39.0 2.0 39.0 10-11 29.567314479593136 35.0 27.0 39.0 2.0 39.0 12-13 29.444460649166345 35.0 27.0 39.0 2.0 39.0 14-15 29.947288826870178 36.0 27.0 40.0 2.0 41.0 16-17 30.017109075020215 36.0 27.0 40.0 2.0 41.0 18-19 29.809786576034305 36.5 27.0 40.0 2.0 41.0 20-21 29.501263579394905 36.0 25.0 40.0 2.0 41.0 22-23 29.36237990842647 36.0 24.5 40.0 2.0 41.0 24-25 29.16831935384384 36.0 24.0 40.0 2.0 41.0 26-27 29.004998702974056 36.0 23.5 40.0 2.0 41.0 28-29 29.030235483129523 36.0 24.5 40.0 2.0 41.0 30-31 28.94619695270853 36.0 24.5 40.0 2.0 41.0 32-33 28.88037298343579 35.0 24.0 40.0 2.0 41.0 34-35 28.409703659713287 35.0 22.0 40.0 2.0 41.0 36-37 28.278603700043597 35.0 21.0 40.0 2.0 41.0 38-39 28.061416220096937 35.0 19.5 40.0 2.0 41.0 40-41 28.248681681054514 35.0 22.0 40.0 2.0 41.0 42-43 28.1245487146998 35.0 21.5 40.0 2.0 41.0 44-45 27.97875210936865 35.0 20.5 39.0 2.0 41.0 46-47 27.916950670622693 35.0 21.0 39.0 2.0 41.0 48-49 27.7520862283069 35.0 19.5 39.0 2.0 41.0 50-51 27.59482951401002 34.0 18.5 39.0 2.0 41.0 52-53 27.521772340250934 34.0 18.5 39.0 2.0 40.5 54-55 27.527718824999504 34.0 18.0 39.0 2.0 41.0 56-57 27.401230794001556 34.0 17.5 39.0 2.0 41.0 58-59 27.202885775767356 34.0 15.0 39.0 2.0 41.0 60-61 26.999693536299418 34.0 13.5 39.0 2.0 40.0 62-63 26.722585596906114 34.0 12.5 38.5 2.0 40.0 64-65 26.45876215888898 33.0 9.0 38.0 2.0 40.0 66-67 26.23034889025527 33.0 8.0 38.0 2.0 40.0 68-69 26.192526485969758 33.0 8.0 37.5 2.0 40.0 70-71 25.980379128266335 33.0 7.0 37.0 2.0 40.0 72-73 25.719543127959874 33.0 4.0 36.5 2.0 39.0 74-75 25.44248788632219 33.0 2.0 36.0 2.0 39.0 76-77 25.15571408959085 32.5 2.0 36.0 2.0 39.0 78-79 24.863644460104688 32.0 2.0 35.0 2.0 37.5 80-81 24.576278153921784 32.0 2.0 35.0 2.0 37.0 82-83 24.33925881676232 32.0 2.0 35.0 2.0 36.5 84-85 24.108533652864523 32.0 2.0 35.0 2.0 36.0 86-87 23.91654056152084 32.0 2.0 35.0 2.0 36.0 88-89 23.725267737656182 32.0 2.0 35.0 2.0 35.5 90-91 23.586402734527375 32.0 2.0 35.0 2.0 35.0 92-93 23.458681848287245 32.0 2.0 35.0 2.0 35.0 94-95 23.330001123959512 31.5 2.0 35.0 2.0 35.0 96-97 23.209757679774214 31.0 2.0 35.0 2.0 35.0 98-99 23.108940456203108 31.0 2.0 35.0 2.0 35.0 100-101 23.006481046114622 31.0 2.0 35.0 2.0 35.0 102-103 22.024082485405053 29.5 2.0 34.0 2.0 35.0 104-105 22.7601729030863 31.0 2.0 34.5 2.0 35.0 106-107 22.878949755120722 31.0 2.0 35.0 2.0 35.0 108-109 22.824813603565868 31.0 2.0 35.0 2.0 35.0 110-111 22.731049323154693 31.0 2.0 35.0 2.0 35.0 112-113 22.62472994830953 31.0 2.0 35.0 2.0 35.0 114-115 22.485900530750015 31.0 2.0 35.0 2.0 35.0 116-117 22.360980403824264 31.0 2.0 35.0 2.0 35.0 118-119 22.19925099182621 31.0 2.0 35.0 2.0 35.0 120-121 21.999681479986325 31.0 2.0 35.0 2.0 35.0 122-123 21.740789268481258 31.0 2.0 35.0 2.0 35.0 124-125 20.449447684740637 28.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7446610742275688 1101 2 0.8061951363124855 1101 3 0.8727564011086031 1101 4 0.9333548238106602 1101 5 0.9176727078216018 1101 6 1.0515222138131826 1101 7 1.0334983788716592 1101 8 0.9371705775715959 1101 9 1.1573427633677866 1101 10-11 1.2058103596915508 1101 12-13 1.0431953196799135 1101 14-15 1.282623169371302 1101 16-17 1.0192480340074965 1101 18-19 1.5312524910721557 1101 20-21 1.8674783085208908 1101 22-23 1.7112687182108282 1101 24-25 1.9001209214894565 1101 26-27 2.069554410151131 1101 28-29 1.7866684361883785 1101 30-31 1.7471902661984622 1101 32-33 1.6961665488862039 1101 34-35 1.9891625042812358 1101 36-37 1.8726942654543564 1101 38-39 1.6769136087175198 1101 40-41 1.6310982070128937 1101 42-43 1.6549639300398056 1101 44-45 1.7195085354898332 1101 46-47 1.6417752554666585 1101 48-49 1.555174335699654 1101 50-51 1.5209818682209608 1101 52-53 1.3023755350859858 1101 54-55 1.2321071520178357 1101 56-57 1.143587743741243 1101 58-59 1.1895750386873374 1101 60-61 1.1424559836566992 1101 62-63 1.1348562408065277 1101 64-65 1.2951465683283843 1101 66-67 1.2045804793421624 1101 68-69 0.9997539228006289 1101 70-71 0.9680087999539921 1101 72-73 0.9382825285718219 1101 74-75 0.9084269594775769 1101 76-77 1.1065939735022283 1101 78-79 1.048943655963427 1101 80-81 1.1925202030232995 1101 82-83 1.0166303305264428 1101 84-85 1.0794197292905778 1101 86-87 1.1117612800859753 1101 88-89 1.0765479795510764 1101 90-91 1.1185397942180906 1101 92-93 0.9731143056226976 1101 94-95 1.1504223533514732 1101 96-97 1.141099835278581 1101 98-99 1.1550882225374934 1101 100-101 1.1379538010035262 1101 102-103 1.1155677016413321 1101 104-105 1.2092525949113124 1101 106-107 1.2033461695266006 1101 108-109 1.296478428751982 1101 110-111 1.1383340503808483 1101 112-113 1.2112312163178593 1101 114-115 1.3041867064060746 1101 116-117 1.3588743674344634 1101 118-119 1.3293307875803961 1101 120-121 1.3640402985570539 1101 122-123 1.53404381736161 1101 124-125 1.6576547046924475 1102 1 -0.27628687491357695 1102 2 -0.011472672145941942 1102 3 0.18453759625675303 1102 4 0.27871490301563995 1102 5 0.36434088086745575 1102 6 -0.4008740206754773 1102 7 -0.9375758659739049 1102 8 0.027425068328103208 1102 9 0.3327614385400395 1102 10-11 0.34482064127530165 1102 12-13 0.4295002588571286 1102 14-15 0.5666300888913547 1102 16-17 0.5469648652842913 1102 18-19 0.7466872288311883 1102 20-21 0.9630467428907892 1102 22-23 0.8095404482764117 1102 24-25 0.944992600905767 1102 26-27 0.46528745465262844 1102 28-29 1.0272672109999732 1102 30-31 0.7915388063205491 1102 32-33 0.5370924972501463 1102 34-35 1.0553442642124224 1102 36-37 1.2115191969031862 1102 38-39 1.2924018611430057 1102 40-41 1.1539834362008499 1102 42-43 0.9605233970872149 1102 44-45 1.0851610705731893 1102 46-47 0.7734433029809153 1102 48-49 0.41130297939076144 1102 50-51 0.8598047529589401 1102 52-53 0.6078038761913653 1102 54-55 0.48581279024231705 1102 56-57 0.5047024479669631 1102 58-59 0.7191143977557424 1102 60-61 0.6583396879086187 1102 62-63 0.4764427309866832 1102 64-65 0.5435366255707912 1102 66-67 0.612533206325125 1102 68-69 0.3934478975021918 1102 70-71 0.3828420230378349 1102 72-73 0.5584948625426414 1102 74-75 0.25657356728629566 1102 76-77 0.4061459348515939 1102 78-79 0.3960150583369533 1102 80-81 0.057912260038659014 1102 82-83 0.1742786433817507 1102 84-85 -0.2464698531149132 1102 86-87 0.2336869839361917 1102 88-89 0.45353888342225446 1102 90-91 -0.09912094626551848 1102 92-93 0.3856418407060538 1102 94-95 0.30264947494465844 1102 96-97 0.5434269728088168 1102 98-99 0.5434659340200341 1102 100-101 0.4788311740965341 1102 102-103 0.32943834296589714 1102 104-105 0.5921343419618665 1102 106-107 0.4711600585610327 1102 108-109 0.07057872195013459 1102 110-111 0.3038806204209301 1102 112-113 0.13265689506175704 1102 114-115 0.18031103290175565 1102 116-117 0.10707053293532454 1102 118-119 0.37754133463619155 1102 120-121 0.7400377289798001 1102 122-123 0.647815561617719 1102 124-125 0.9370995057194058 1103 1 0.48804169261924457 1103 2 0.6201265887746779 1103 3 0.5264781248543393 1103 4 0.5672549986284707 1103 5 0.6004347239132635 1103 6 0.5943673210207301 1103 7 0.7675910112487614 1103 8 0.7568697013763064 1103 9 0.8885722275406671 1103 10-11 1.0777006299219742 1103 12-13 0.997080803482941 1103 14-15 1.2969583614602662 1103 16-17 1.1296333703738135 1103 18-19 1.057617728655881 1103 20-21 1.481042880533188 1103 22-23 1.4140393435530925 1103 24-25 1.5414125460313493 1103 26-27 1.4388353139381493 1103 28-29 1.3312148576888632 1103 30-31 1.3412044675127568 1103 32-33 1.340186279077031 1103 34-35 1.7237296985606925 1103 36-37 1.5966697119769577 1103 38-39 1.6656807745627518 1103 40-41 1.4618943125775772 1103 42-43 1.3628051933617655 1103 44-45 0.965285790218342 1103 46-47 1.0242970287090039 1103 48-49 1.0486303837640207 1103 50-51 1.0500964054803212 1103 52-53 0.8255492945114007 1103 54-55 0.7205136244892962 1103 56-57 0.7699687781150573 1103 58-59 0.7528905479758485 1103 60-61 0.7347175311102561 1103 62-63 0.7002941804838549 1103 64-65 0.9356641501557554 1103 66-67 1.064542856653759 1103 68-69 1.0962453240812238 1103 70-71 1.0881693216693122 1103 72-73 1.0742549789699005 1103 74-75 1.0720253629486578 1103 76-77 1.0700415176279279 1103 78-79 1.1510082964284827 1103 80-81 1.101840177537813 1103 82-83 1.2327343681859908 1103 84-85 1.0979912255625344 1103 86-87 0.9869249085033438 1103 88-89 0.9297074692094505 1103 90-91 0.989532543491439 1103 92-93 1.1112328815947272 1103 94-95 1.0690995564771306 1103 96-97 1.2081545764218475 1103 98-99 1.172113607979174 1103 100-101 1.2167133054055626 1103 102-103 1.059744214973822 1103 104-105 1.1275837166940335 1103 106-107 1.150967831138292 1103 108-109 1.2785697394643094 1103 110-111 1.2276092146674635 1103 112-113 1.2954789205176986 1103 114-115 1.3542993901753988 1103 116-117 1.4080745232961291 1103 118-119 1.2665860689738864 1103 120-121 1.237569354119632 1103 122-123 1.3292976717783915 1103 124-125 1.4873858433250788 1104 1 0.824443674865126 1104 2 0.8263342311304847 1104 3 0.7663054657785828 1104 4 0.8548132034780451 1104 5 0.8595435532017888 1104 6 0.799013997241655 1104 7 0.835346180784331 1104 8 0.9883341800130907 1104 9 1.326787156329953 1104 10-11 1.191692760783802 1104 12-13 1.117176897261647 1104 14-15 1.1858919593730306 1104 16-17 1.256099975095296 1104 18-19 1.5267824136016266 1104 20-21 1.805893792124813 1104 22-23 1.5178252179513585 1104 24-25 1.9864516817385471 1104 26-27 2.096486462811324 1104 28-29 1.9809393853568338 1104 30-31 1.896690553469572 1104 32-33 1.8760411226129463 1104 34-35 2.1460468946486984 1104 36-37 2.1933278218089143 1104 38-39 2.1207436121771295 1104 40-41 1.9543515677759835 1104 42-43 1.9021336918210139 1104 44-45 1.739377348349695 1104 46-47 1.5209665564032235 1104 48-49 1.5478925536303123 1104 50-51 1.375616508792291 1104 52-53 1.539152955576757 1104 54-55 1.5042803116293726 1104 56-57 1.4166308251408992 1104 58-59 1.4249747934257648 1104 60-61 1.3164229660126914 1104 62-63 1.3622429077203115 1104 64-65 1.4092190687608372 1104 66-67 1.4135821813462641 1104 68-69 1.4088368638390065 1104 70-71 1.4461395144868234 1104 72-73 1.2217696909510458 1104 74-75 1.3843378868999174 1104 76-77 1.3400936460749406 1104 78-79 1.3568689407633876 1104 80-81 1.2934183885819053 1104 82-83 1.2763689755952647 1104 84-85 1.2649559378061568 1104 86-87 1.2967264214357144 1104 88-89 1.30881632474593 1104 90-91 1.3707248307911009 1104 92-93 1.3774974295261266 1104 94-95 1.3866074702368394 1104 96-97 1.23027898124937 1104 98-99 1.2864518181833269 1104 100-101 1.3346152010331807 1104 102-103 1.2967778543583606 1104 104-105 1.297634795222681 1104 106-107 1.3682803288415961 1104 108-109 1.39148832879399 1104 110-111 1.4804418944266153 1104 112-113 1.469995301869048 1104 114-115 1.4028535213417008 1104 116-117 1.4323061142899292 1104 118-119 1.3780174489340915 1104 120-121 1.321280920545341 1104 122-123 1.2585207059374852 1104 124-125 1.3395884408231211 1105 1 -0.1417029041364941 1105 2 0.14534342623488072 1105 3 0.2227286919982241 1105 4 0.20159931416020882 1105 5 0.2659916625135743 1105 6 0.23319071737592978 1105 7 0.45496873635031676 1105 8 0.10930732877699612 1105 9 -0.01705436889495715 1105 10-11 0.30757806731788406 1105 12-13 0.3603703239867606 1105 14-15 0.40391075179811864 1105 16-17 0.5030741380650632 1105 18-19 0.5696919739080748 1105 20-21 0.8918183166883331 1105 22-23 1.0290945168973984 1105 24-25 0.998498868306644 1105 26-27 0.8262192230575565 1105 28-29 0.5543126273418011 1105 30-31 0.5921222815848033 1105 32-33 0.8267139166763897 1105 34-35 1.3206936103521265 1105 36-37 1.2337834423484857 1105 38-39 1.3679170080130625 1105 40-41 0.9162892312329411 1105 42-43 0.6189202279988812 1105 44-45 0.8268738790724264 1105 46-47 0.7209196290124531 1105 48-49 0.5873252385288907 1105 50-51 0.5590774843698689 1105 52-53 0.6432901835019997 1105 54-55 0.44264767944570593 1105 56-57 0.5330012485078299 1105 58-59 0.4990847314275513 1105 60-61 0.5734382132438007 1105 62-63 0.5647190469818177 1105 64-65 0.612474744601915 1105 66-67 0.43306878521545755 1105 68-69 0.28629060961088726 1105 70-71 0.43136210693772625 1105 72-73 0.5427855703471955 1105 74-75 0.6401087208637364 1105 76-77 0.6605834756930093 1105 78-79 0.7196399485467246 1105 80-81 0.5304047512506465 1105 82-83 0.47716527980943013 1105 84-85 0.5254681065952589 1105 86-87 0.5195753983966078 1105 88-89 0.6545538277819496 1105 90-91 0.4876380845037147 1105 92-93 0.38922597930485736 1105 94-95 0.5470967217498028 1105 96-97 0.616389042631976 1105 98-99 0.5214539353154386 1105 100-101 0.5193688946333381 1105 102-103 0.43941384291420604 1105 104-105 0.5662386895041998 1105 106-107 0.4182631732169959 1105 108-109 0.4544355681028627 1105 110-111 0.49253382917497035 1105 112-113 0.5435452409675534 1105 114-115 0.5562887589558336 1105 116-117 0.6420255419063849 1105 118-119 0.7155833652022281 1105 120-121 0.611635534354317 1105 122-123 0.754536533601641 1105 124-125 0.7229956668523911 1106 1 0.1797624089921932 1106 2 0.3624418077223943 1106 3 0.14714040390461847 1106 4 0.13768578760392103 1106 5 0.13837019307178622 1106 6 0.12004124086629986 1106 7 -0.13503333843118526 1106 8 0.19331546693758384 1106 9 0.28999670025771707 1106 10-11 0.44174979703500483 1106 12-13 0.6000824846365198 1106 14-15 0.8199196312880694 1106 16-17 0.6382227658479565 1106 18-19 0.6948088763481373 1106 20-21 1.0553791695855423 1106 22-23 0.9736369008422443 1106 24-25 1.18024401171089 1106 26-27 1.291978991580926 1106 28-29 1.1442365058557655 1106 30-31 1.0050723935419974 1106 32-33 0.9395123592314647 1106 34-35 1.2410682521322158 1106 36-37 1.0854604746479204 1106 38-39 1.2705944364546582 1106 40-41 0.8623392614270209 1106 42-43 0.8544450340499239 1106 44-45 0.8043264720136243 1106 46-47 0.6387617112255377 1106 48-49 0.6091012658658315 1106 50-51 0.5827199331451105 1106 52-53 0.6028207080062415 1106 54-55 0.438710918733598 1106 56-57 0.4901146460304773 1106 58-59 0.38912232443015426 1106 60-61 0.40049491879912225 1106 62-63 0.36194955991014055 1106 64-65 0.4444123801715101 1106 66-67 0.35683474097766776 1106 68-69 0.3338620634918108 1106 70-71 0.38803435208387427 1106 72-73 0.467244382929362 1106 74-75 0.39334896645097217 1106 76-77 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-3.0359140988086715 2216 74-75 -2.8450677018771593 2216 76-77 -2.878182098662979 2216 78-79 -3.0986266928038972 2216 80-81 -3.1315633084715806 2216 82-83 -2.9161875626166953 2216 84-85 -2.898578214338823 2216 86-87 -2.90043709146504 2216 88-89 -2.899728838889189 2216 90-91 -2.912739631057093 2216 92-93 -2.9206413409296026 2216 94-95 -2.953326307455658 2216 96-97 -2.9379160022571718 2216 98-99 -2.898867625597319 2216 100-101 -2.8852363269388803 2216 102-103 -2.690664267551746 2216 104-105 -2.949577468072487 2216 106-107 -2.9946703403483674 2216 108-109 -3.056945987584122 2216 110-111 -2.936891352593374 2216 112-113 -2.8978062128706767 2216 114-115 -3.0986062560459615 2216 116-117 -3.0323918769869564 2216 118-119 -3.0886979384047635 2216 120-121 -3.251739603008094 2216 122-123 -3.30670569895484 2216 124-125 -3.229623773965372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 321785.0 3 46673.0 4 27983.0 5 21712.0 6 19414.0 7 19850.0 8 20769.0 9 21517.0 10 22099.0 11 23259.0 12 24600.0 13 26228.0 14 28227.0 15 24755.0 16 19517.0 17 17480.0 18 15679.0 19 14696.0 20 14721.0 21 15628.0 22 19124.0 23 24861.0 24 33545.0 25 41631.0 26 48999.0 27 54735.0 28 61620.0 29 70099.0 30 77971.0 31 87752.0 32 103789.0 33 125726.0 34 157375.0 35 206211.0 36 290311.0 37 329952.0 38 90773.0 39 201.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.559686865618346 18.960414390396547 13.044024506507231 23.435874237477872 2 14.990461411011092 20.079731324881813 41.083140325187124 23.846666938919974 3 19.09633385426431 25.770642051965005 28.4561721763739 26.67685191739679 4 11.966505422118571 16.680428915424596 38.096484578437625 33.25658108401921 5 13.349374645546902 38.7859013824935 33.97747525927102 13.887248712688585 6 31.201536067005755 37.21384451560763 17.795991198028034 13.788628219358582 7 28.35878423737133 32.7897385315356 20.85151301352654 17.99996421756653 8 25.60947579539581 36.30486448898539 19.702076836050086 18.38358287956871 9 26.29892657125078 16.868971686372124 19.7241365899191 37.10796515245799 10-11 24.628636388208612 27.52327937938767 27.280344670545688 20.567739561858026 12-13 25.405430527890527 24.985138184258513 27.507845113530273 22.10158617432069 14-15 22.430543467161982 26.215646136117204 25.43288226742066 25.920928129300158 16-17 21.950559101609322 29.58982486812687 25.925212695144808 22.534403335119006 18-19 21.841916117359155 28.225953243649837 28.315311327843627 21.616819311147378 20-21 22.737802511180206 27.267778840027972 28.552928711948606 21.44148993684322 22-23 22.31816334428155 27.13539813199699 28.24995770449149 22.29648081922997 24-25 22.0395075260256 27.198088198715002 28.57884653644517 22.18355773881423 26-27 21.983513123684247 27.473284289699745 28.261354954854028 22.281847631761977 28-29 21.90262694625252 27.445230549822842 28.369214961329202 22.282927542595438 30-31 22.203340160822272 27.369052126351523 28.134699297824557 22.29290841500165 32-33 22.102167136904026 27.360074242272198 27.966054230152597 22.571704390671176 34-35 22.264371470883663 27.01053763629062 28.540707053322173 22.184383839503543 36-37 22.307916408008825 26.971925705688605 28.290438917075463 22.429718969227107 38-39 22.070247858351543 27.220627029398347 28.404829214546755 22.304295897703348 40-41 22.494573770367445 27.22357738837255 27.971316597134035 22.31053224412597 42-43 22.261484717414078 27.289927769757817 27.885334757933105 22.563252754895004 44-45 22.35606218261524 27.391585936755465 27.757034247894154 22.495317632735144 46-47 22.402101456166154 27.77798196501631 27.195056568615954 22.624860010201584 48-49 22.517671420215976 27.644031727155333 27.14307971409849 22.695217138530204 50-51 22.411845100025047 27.717668628430168 27.125191937011987 22.745294334532794 52-53 22.62876628525937 27.781245588264465 26.945315286199374 22.644672840276797 54-55 22.596951730053163 27.546515144590067 27.070399223588744 22.78613390176803 56-57 22.465697269433026 27.8217809844696 26.98592964785032 22.72659209824705 58-59 22.578420195075527 27.55537604999571 26.991119725130925 22.875084029797836 60-61 22.695443285067856 27.562796035954957 27.17324446558491 22.568516213392275 62-63 22.62271415383574 27.624587296011317 27.147548751296902 22.605149798856043 64-65 22.475351721640404 27.48786038376494 27.14281463277162 22.893973261823035 66-67 22.4561238387781 27.75509934217844 26.924732830483965 22.86404398855949 68-69 22.42759654086301 27.551241899576727 27.149036118191976 22.872125441368283 70-71 22.299590112449746 27.521096238205377 27.321966884632932 22.857346764711945 72-73 22.52526996966476 27.59083555917551 26.99375752886983 22.890136942289914 74-75 22.31074542866149 27.806405056187312 26.998184357080202 22.884665158070998 76-77 22.561251066413313 27.568332614774466 27.042685897207775 22.82773042160445 78-79 22.526733691383594 27.69672848572508 26.926711056394765 22.849826766496562 80-81 22.34935551142547 27.80821768631285 27.040517488816445 22.801909313445236 82-83 22.559418268596904 27.804844468834066 26.890395652493503 22.745341610075524 84-85 22.562923259233678 27.66128527298021 26.830780311807366 22.945011155978744 86-87 22.410484795789838 27.596914721967742 27.052170197853965 22.94043028438846 88-89 22.572845462866116 27.53970621813516 27.052546289723235 22.834902029275494 90-91 22.569650765395487 27.75050964449075 27.010545327871448 22.66929426224231 92-93 22.510283816414553 27.645006527231086 27.02608150472658 22.81862815162778 94-95 22.475122733037168 27.541935408578478 27.01869587881523 22.96424597956913 96-97 22.498651683513895 27.619006312630102 26.93388843972019 22.94845356413581 98-99 22.368111454265506 27.47291874008019 26.899907943356578 23.259061862297727 100-101 22.37207102506538 27.57526537940348 27.01249612996473 23.040167465566412 102-103 22.413658324864745 27.441393678680587 27.064925579490577 23.080022416964088 104-105 22.410701194410297 27.412437719423448 27.18447262727095 22.992388458895306 106-107 22.229574847565363 27.53371296474188 27.235338545292475 23.00137364240028 108-109 22.31784952710084 27.572068556085384 26.86051273555022 23.249569181263556 110-111 22.188738639494858 27.613137378859687 27.01465263791625 23.183471343729213 112-113 22.204772977679877 27.632700143547908 27.130554703187183 23.031972175585032 114-115 22.28786430969635 27.730259051277056 26.96522764847058 23.016648990556018 116-117 22.041779402916927 27.690687120396284 27.046316076860162 23.22121739982662 118-119 22.073534175952943 27.64090621471827 27.010224920243598 23.275334689085188 120-121 22.12227792112483 27.65494267317008 27.085651015035207 23.137128390669886 122-123 22.178988024191966 27.796296533965553 26.900979167079885 23.123736274762596 124-125 22.178133358644285 27.744552828576897 26.770534937674366 23.30677887510445 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1700.0 1 1754.5 2 1742.0 3 1430.5 4 1020.5 5 800.0 6 775.0 7 978.0 8 1310.0 9 1490.5 10 1402.0 11 1276.0 12 1389.5 13 1609.0 14 1803.5 15 1960.0 16 2097.5 17 2247.5 18 2476.5 19 2942.5 20 3449.5 21 3987.0 22 4803.0 23 6037.0 24 7533.0 25 9604.5 26 12909.5 27 16795.5 28 20528.0 29 24831.5 30 30241.5 31 35660.5 32 41323.5 33 47486.5 34 54009.5 35 60232.5 36 66472.0 37 72605.5 38 75719.5 39 77565.0 40 79927.5 41 82834.0 42 84824.5 43 86849.5 44 89658.5 45 91301.5 46 91740.0 47 91167.5 48 92226.5 49 93376.0 50 91616.5 51 89266.0 52 87473.5 53 85688.0 54 82487.0 55 76083.0 56 69356.5 57 63885.5 58 59372.5 59 54302.0 60 47744.5 61 39889.5 62 31785.0 63 24707.0 64 18636.5 65 14312.0 66 11190.0 67 8784.5 68 6761.5 69 5042.0 70 3725.5 71 2797.0 72 2211.5 73 1847.5 74 1446.5 75 1115.0 76 831.5 77 548.0 78 365.5 79 238.0 80 161.5 81 127.5 82 98.5 83 73.0 84 51.5 85 32.5 86 25.0 87 24.0 88 20.5 89 17.5 90 14.0 91 11.5 92 12.0 93 7.5 94 3.0 95 3.0 96 2.5 97 2.0 98 3.5 99 3.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013923097056820627 2 0.0051336558980455945 3 0.008906115156457886 4 0.008711658493653128 5 0.015750989687185343 6 9.722833140237868E-4 7 0.006728200533044604 8 0.0 9 0.0026057192815837483 10-11 0.0 12-13 0.003130752271156593 14-15 0.019620677277000016 16-17 0.031268631379004985 18-19 0.005872591216703672 20-21 0.00797272317499505 22-23 0.0028001759443885056 24-25 0.011220149443834497 26-27 0.019251209617670975 28-29 4.4725032445094186E-4 30-31 0.0016917729664013888 32-33 0.00875054982621408 34-35 0.009508930811152633 36-37 0.011784073765968295 38-39 0.0 40-41 0.02533770316345988 42-43 0.009820061471640246 44-45 0.017578882317550063 46-47 0.003791904924692768 48-49 0.025726616489069397 50-51 0.004316937914265614 52-53 0.0 54-55 0.01806502397456196 56-57 0.00451139457707037 58-59 2.5279366164618453E-4 60-61 0.0041030355851803795 62-63 0.02800175944388506 64-65 0.017442762653586734 66-67 0.0020612406257304277 68-69 0.022090276894620436 70-71 0.0028779586095104086 72-73 0.012834139745113984 74-75 5.8336998841427204E-5 76-77 0.005775362885301293 78-79 0.0010306203128652139 80-81 0.0031501979374370685 82-83 0.0017890012978037675 84-85 0.0 86-87 0.0059698195481060505 88-89 0.017423316987306255 90-91 0.02343202786797326 92-93 0.004491948910789895 94-95 0.025162692166935598 96-97 0.018220589304805763 98-99 9.333919814628353E-4 100-101 0.00898389782157979 102-103 0.0 104-105 0.0015556533024380587 106-107 3.889133256095147E-5 108-109 0.0 110-111 9.139463151823595E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 5.8336998841427204E-5 122-123 0.0 124-125 0.022362516222547094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2571267.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.81027518194131 #Duplication Level Percentage of deduplicated Percentage of total 1 82.19965675050209 51.629830603601555 2 9.52966492582733 11.97121752765818 3 3.4025725030334435 6.411495457261123 4 1.577807070599181 3.964099851533889 5 0.895700051192287 2.812958334793323 6 0.5323000439032647 2.0060347342154095 7 0.35202873067221496 1.5477715011829927 8 0.25473444669012274 1.2799952555940934 9 0.1960660201874827 1.1083464613623464 >10 0.9682960528974741 11.017528650885279 >50 0.0619301632002751 2.6779289083967264 >100 0.028738375416253563 3.0049718318780703 >500 2.5243293933407257E-4 0.10686527727139995 >1k 2.524329393288564E-4 0.46095560436557803 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4467 0.1737275825497702 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3260 0.12678574414870178 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.889133256095147E-5 0.0 2 3.889133256095147E-5 0.0 0.0 3.889133256095147E-5 0.0 3 3.889133256095147E-5 0.0 0.0 3.889133256095147E-5 0.0 4 3.889133256095147E-5 0.0 0.0 3.889133256095147E-5 0.0 5 3.889133256095147E-5 0.0 0.0 3.889133256095147E-5 0.0 6 3.889133256095147E-5 0.0 0.0 3.889133256095147E-5 0.0 7 3.889133256095147E-5 0.0 0.0 3.889133256095147E-5 0.0 8 3.889133256095147E-5 0.0 0.0 3.889133256095147E-5 0.0 9 3.889133256095147E-5 0.0 0.0 3.889133256095147E-5 0.0 10-11 3.889133256095147E-5 0.0 0.0 3.889133256095147E-5 0.0 12-13 7.778266512190294E-5 0.0 0.0 7.778266512190294E-5 0.0 14-15 1.1667399768285441E-4 0.0 0.0 7.778266512190294E-5 0.0 16-17 1.1667399768285441E-4 0.0 0.0 1.1667399768285441E-4 0.0 18-19 1.5556533024380589E-4 0.0 0.0 1.7501099652428162E-4 0.0 20-21 1.5556533024380589E-4 1.9445666280475736E-5 0.0 2.722393279266603E-4 0.0 22-23 1.5556533024380589E-4 3.889133256095147E-5 0.0 4.2780465817046615E-4 0.0 24-25 1.5556533024380589E-4 3.889133256095147E-5 0.0 6.61152653536175E-4 0.0 26-27 1.5556533024380589E-4 3.889133256095147E-5 0.0 7.778266512190293E-4 0.0 28-29 1.9445666280475734E-4 3.889133256095147E-5 0.0 0.0012250769756699713 0.0 30-31 2.722393279266603E-4 3.889133256095147E-5 0.0 0.0030335239397542143 0.0 32-33 2.9168499420713606E-4 3.889133256095147E-5 0.0 0.006397624206276517 0.0 34-35 3.500219930485632E-4 3.889133256095147E-5 0.0 0.011142366778712595 0.0 36-37 3.500219930485632E-4 3.889133256095147E-5 0.0 0.019328992282792883 0.0 38-39 3.500219930485632E-4 3.889133256095147E-5 0.0 0.034399383650161576 0.0 40-41 3.694676593290389E-4 3.889133256095147E-5 0.0 0.05372837593295446 0.0 42-43 4.4725032445094186E-4 3.889133256095147E-5 0.0 0.07126836691794355 0.0 44-45 5.055873232923691E-4 3.889133256095147E-5 0.0 0.09112239219030929 0.0 46-47 6.417069872556992E-4 3.889133256095147E-5 0.0 0.11891024930510911 0.0 48-49 6.61152653536175E-4 3.889133256095147E-5 0.0 0.14870101004679792 0.0 50-51 6.61152653536175E-4 3.889133256095147E-5 0.0 0.1840726770109833 0.0 52-53 6.61152653536175E-4 3.889133256095147E-5 0.0 0.2207083122833996 0.0 54-55 6.61152653536175E-4 3.889133256095147E-5 0.0 0.2584329048675225 0.0 56-57 6.61152653536175E-4 3.889133256095147E-5 0.0 0.30245789332651957 0.0 58-59 6.61152653536175E-4 3.889133256095147E-5 0.0 0.3562640519245959 0.0 60-61 6.61152653536175E-4 3.889133256095147E-5 0.0 0.41751790070809447 0.0 62-63 6.61152653536175E-4 3.889133256095147E-5 0.0 0.4798412611370192 0.0 64-65 7.000439860971264E-4 5.8336998841427204E-5 0.0 0.5431174592136873 0.0 66-67 7.778266512190293E-4 7.778266512190294E-5 0.0 0.6184110790516892 0.0 68-69 7.778266512190293E-4 7.778266512190294E-5 0.0 0.7039720106857825 0.0 70-71 8.167179837799809E-4 7.778266512190294E-5 0.0 0.8080257709526082 0.0 72-73 8.945006489018837E-4 7.778266512190294E-5 0.0 0.916785382459309 0.0 74-75 8.945006489018837E-4 7.778266512190294E-5 0.0 1.0195946200841841 0.0 76-77 8.945006489018837E-4 7.778266512190294E-5 0.0 1.1390882393777075 0.0 78-79 8.945006489018837E-4 7.778266512190294E-5 0.0 1.2772496983004875 0.0 80-81 0.001166739976828544 7.778266512190294E-5 0.0 1.4295481566091737 0.0 82-83 0.0012056313093894956 7.778266512190294E-5 0.0 1.5996199538982143 0.0 84-85 0.0012250769756699713 7.778266512190294E-5 0.0 1.7693222835279263 0.0 86-87 0.001244522641950447 7.778266512190294E-5 0.0 1.9466473143395844 0.0 88-89 0.001244522641950447 7.778266512190294E-5 0.0 2.143573576761962 0.0 90-91 0.0012834139745113984 7.778266512190294E-5 0.0 2.3788661387557184 0.0 92-93 0.00132230530707235 7.778266512190294E-5 0.0 2.6266622641678206 0.0 94-95 0.00132230530707235 7.778266512190294E-5 0.0 2.878289185837177 0.0 96-97 0.00132230530707235 7.778266512190294E-5 0.0 3.135438676730188 0.0 98-99 0.001400087972194253 1.1667399768285441E-4 0.0 3.4195009697553775 3.889133256095147E-5 100-101 0.0015167619698771072 1.1667399768285441E-4 0.0 3.750894014507245 3.889133256095147E-5 102-103 0.0015945446349990102 1.1667399768285441E-4 0.0 4.1087915023994 3.889133256095147E-5 104-105 0.001672327300120913 1.1667399768285441E-4 0.0 4.479270336375024 3.889133256095147E-5 106-107 0.0019834579606085247 1.1667399768285441E-4 0.0 4.848290745379613 3.889133256095147E-5 108-109 0.0020223492931694762 1.1667399768285441E-4 0.0 5.230962012113094 3.889133256095147E-5 110-111 0.0020612406257304277 1.1667399768285441E-4 3.889133256095147E-5 5.6574054736439265 3.889133256095147E-5 112-113 0.0021001319582913793 1.1667399768285441E-4 3.889133256095147E-5 6.127309999311624 3.889133256095147E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3525 0.0 57.554752 1 GTATCAA 6860 0.0 52.6442 1 ATCAACG 8360 0.0 42.69947 3 TCAACGC 8545 0.0 42.053524 4 CAACGCA 8625 0.0 41.73244 5 AACGCAG 9080 0.0 39.83469 6 TATCAAC 9020 0.0 39.77299 2 ACGCAGA 10445 0.0 34.628914 7 GTACATG 11370 0.0 34.483463 1 CGCAGAG 10500 0.0 33.94091 8 TACATGG 11630 0.0 32.279526 2 ACATGGG 11900 0.0 30.997183 3 GCAGAGT 11360 0.0 28.648043 9 CATGGGG 5895 0.0 26.542965 4 GAGTACT 5315 0.0 24.520912 12-13 CAGAGTA 11050 0.0 23.666916 10-11 AGAGTAC 10680 0.0 23.483965 10-11 GAGTACA 8785 0.0 22.687622 1 CATGGGA 6455 0.0 22.48905 4 AGTACAT 8580 0.0 21.84242 2 >>END_MODULE