FastQCFastQC Report
Thu 26 May 2016
SRR1364523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571267
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73620.28631799031372474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59880.2328812993749774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39440.1533874156203926No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51400.062.4956361
GTATCAA91000.050.100731
ATCAACG112600.039.982433
TCAACGC114100.039.3524254
CAACGCA115550.038.6201325
TATCAAC119550.037.7576872
AACGCAG120000.037.2871366
GTACATG114950.033.2851141
TACATGG117050.031.8993532
ACGCAGA138750.031.8623837
CGCAGAG141200.031.1409748
ACATGGG117700.030.8630713
GCAGAGT153750.028.0943269
GAGTACT91600.026.53344712-13
CATGGGA65600.025.2363594
GTACTTT100100.024.07232314-15
AGTACTT96050.023.97235712-13
CAGAGTA155000.023.95152310-11
AGAGTAC147100.023.37735610-11
AGTACAT86600.022.3486462
Produced by FastQC (version 0.11.5)