##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364523_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2571267 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.134692352058345 31.0 30.0 34.0 26.0 34.0 2 30.05038411024604 31.0 30.0 34.0 26.0 34.0 3 30.29779326689916 31.0 30.0 34.0 26.0 34.0 4 33.87336359856833 35.0 35.0 37.0 30.0 37.0 5 33.289621420101454 35.0 33.0 37.0 27.0 37.0 6 33.314585377559 36.0 33.0 37.0 27.0 37.0 7 33.10490003566335 36.0 33.0 37.0 26.0 37.0 8 33.04157872364091 36.0 33.0 37.0 26.0 37.0 9 34.2908507751237 38.0 34.0 39.0 25.0 39.0 10-11 34.223221664650154 38.0 34.0 39.0 25.0 39.0 12-13 34.10559969073613 38.0 34.0 39.0 25.0 39.0 14-15 35.075534746099876 39.0 34.0 41.0 25.0 41.0 16-17 35.087344682602 39.0 34.0 41.0 25.0 41.0 18-19 35.10887161854448 39.0 34.0 41.0 25.0 41.0 20-21 35.12285869184336 39.0 34.0 41.0 25.0 41.0 22-23 35.0787629211591 39.0 34.0 41.0 25.0 41.0 24-25 35.040858067248564 39.0 34.0 41.0 25.0 41.0 26-27 34.920578454123984 39.0 34.0 41.0 24.0 41.0 28-29 34.77589316862076 39.0 34.0 41.0 24.0 41.0 30-31 34.62072064083582 38.5 33.5 41.0 24.0 41.0 32-33 34.42579572638703 38.0 33.0 41.0 23.5 41.0 34-35 34.24522443604651 38.0 33.0 40.0 22.0 41.0 36-37 34.04785247895299 38.0 33.0 40.0 20.5 41.0 38-39 33.88497441144774 38.0 33.0 40.0 19.0 41.0 40-41 33.92260255352711 38.0 33.0 40.0 19.0 41.0 42-43 33.79005972541941 38.0 33.0 40.0 17.0 41.0 44-45 33.6338853569077 38.0 33.0 40.0 15.5 41.0 46-47 33.40284186745289 38.0 32.5 40.0 14.0 41.0 48-49 33.132388429517434 38.0 32.0 40.0 9.0 41.0 50-51 32.973927639564465 38.0 32.0 40.0 8.0 41.0 52-53 32.79518249174434 38.0 32.0 40.0 7.5 41.0 54-55 32.61261393702016 38.0 31.0 40.0 6.0 41.0 56-57 32.40428609708755 37.0 31.0 40.0 2.0 41.0 58-59 32.19975580132285 37.0 31.0 40.0 2.0 41.0 60-61 31.965130809052503 37.0 31.0 40.0 2.0 41.0 62-63 31.78672596039229 36.0 31.0 40.0 2.0 41.0 64-65 31.65080328102838 36.0 31.0 39.5 2.0 41.0 66-67 31.348820445329093 35.5 30.0 39.0 2.0 41.0 68-69 31.062053065667627 35.0 30.0 39.0 2.0 41.0 70-71 30.748365688977458 35.0 30.0 38.5 2.0 40.0 72-73 30.45561857247808 35.0 30.0 37.5 2.0 40.0 74-75 30.09815258392069 35.0 29.0 37.0 2.0 39.0 76-77 29.78121486411174 35.0 29.0 36.5 2.0 39.0 78-79 29.490498069628707 35.0 29.0 36.0 2.0 39.0 80-81 29.2034475610662 34.0 29.0 36.0 2.0 37.5 82-83 28.916578480570085 34.0 29.0 35.0 2.0 37.0 84-85 28.68979028626743 34.0 29.0 35.0 2.0 36.5 86-87 28.477214151622526 34.0 29.0 35.0 2.0 36.0 88-89 28.295286331602277 34.0 29.0 35.0 2.0 36.0 90-91 28.146961789654675 34.0 28.5 35.0 2.0 36.0 92-93 28.030520362140532 34.0 28.0 35.0 2.0 35.0 94-95 27.91752334549465 34.0 28.0 35.0 2.0 35.0 96-97 27.8025494824147 34.0 27.5 35.0 2.0 35.0 98-99 27.720868155660224 34.0 27.0 35.0 2.0 35.0 100-101 27.616607687960837 34.0 27.0 35.0 2.0 35.0 102-103 26.34082438735456 31.5 24.5 34.0 2.0 35.0 104-105 27.28302661683909 33.5 26.5 35.0 2.0 35.0 106-107 27.445550578761367 34.0 27.0 35.0 2.0 35.0 108-109 27.452564047218743 34.0 27.0 35.0 2.0 35.0 110-111 27.374683375938787 34.0 27.0 35.0 2.0 35.0 112-113 27.28739644696564 34.0 27.0 35.0 2.0 35.0 114-115 27.185479570966375 34.0 27.0 35.0 2.0 35.0 116-117 27.062742220080608 34.0 26.5 35.0 2.0 35.0 118-119 26.931729376995854 34.0 26.0 35.0 2.0 35.0 120-121 26.735824789879853 34.0 25.0 35.0 2.0 35.0 122-123 26.44804604111514 34.0 25.0 35.0 2.0 35.0 124-125 24.656032415147862 31.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5057050529327007 1101 2 0.6550424438680906 1101 3 0.4544342751936199 1101 4 0.40992687538879835 1101 5 0.4371481026032953 1101 6 0.48669479411866234 1101 7 0.5190517166155431 1101 8 0.5889582639831019 1101 9 0.774485535035744 1101 10-11 0.7570069079044046 1101 12-13 0.6033732946104848 1101 14-15 0.7481113416809748 1101 16-17 0.8183984547706444 1101 18-19 0.8992713299631703 1101 20-21 0.9614311649022014 1101 22-23 0.981530355953268 1101 24-25 0.9653480118468636 1101 26-27 0.9373484651062256 1101 28-29 1.0926931504883157 1101 30-31 1.162600970882636 1101 32-33 1.177777191142333 1101 34-35 1.2516664262738786 1101 36-37 1.179785814139784 1101 38-39 1.1056092121610774 1101 40-41 0.9032834644013406 1101 42-43 0.9691782365719277 1101 44-45 0.9614947115176378 1101 46-47 0.9673329378889264 1101 48-49 1.092207733138082 1101 50-51 1.0629876114904206 1101 52-53 1.0464596868998584 1101 54-55 1.1122943369635934 1101 56-57 1.0714608718323149 1101 58-59 1.1743503417874308 1101 60-61 1.1228987872817697 1101 62-63 1.115137331063103 1101 64-65 1.0966677164881062 1101 66-67 1.218807020154717 1101 68-69 1.252307851848549 1101 70-71 1.2336266478281424 1101 72-73 1.1090197563238 1101 74-75 1.145610728109375 1101 76-77 1.0995727320524686 1101 78-79 1.0849046359398464 1101 80-81 0.8830083443175809 1101 82-83 1.0897500455565954 1101 84-85 0.9922734783400919 1101 86-87 1.143468711950387 1101 88-89 1.1518193210841865 1101 90-91 1.1073538057856176 1101 92-93 1.0171306772366826 1101 94-95 1.014244278632475 1101 96-97 1.0938138466160297 1101 98-99 1.0611011185160812 1101 100-101 1.0067391849668432 1101 102-103 0.8963360645421368 1101 104-105 0.996066678793408 1101 106-107 0.9105288317374054 1101 108-109 1.0226060495085498 1101 110-111 0.9964950458672277 1101 112-113 0.9331484561280909 1101 114-115 1.0100369878912083 1101 116-117 1.0059755253108236 1101 118-119 1.0173340857961044 1101 120-121 1.0424293436042156 1101 122-123 1.0114606521588563 1101 124-125 1.2240976908747534 1102 1 0.4656058938852574 1102 2 -0.24780674137725356 1102 3 0.28482552989832755 1102 4 0.3718686964047535 1102 5 0.48300424994027935 1102 6 0.3961125567307704 1102 7 0.5716603724013538 1102 8 0.6363950178887734 1102 9 0.9282647456930491 1102 10-11 0.9571367512409736 1102 12-13 1.0523945029012438 1102 14-15 1.2109926029788127 1102 16-17 1.1430870272471338 1102 18-19 1.2523978581739428 1102 20-21 1.0642820693846602 1102 22-23 1.2351720003517244 1102 24-25 1.1666053199690865 1102 26-27 1.1861099321449018 1102 28-29 0.7846847256806555 1102 30-31 0.9895339664813889 1102 32-33 1.2094025579129166 1102 34-35 1.1790222450455445 1102 36-37 0.8114717362621633 1102 38-39 0.23214656490706176 1102 40-41 -0.04268336905860792 1102 42-43 0.2334803791234279 1102 44-45 -0.1152615847107441 1102 46-47 0.08273318174770594 1102 48-49 -0.10151535943656143 1102 50-51 -0.1472271949070958 1102 52-53 -0.2639452998702083 1102 54-55 -0.1859863440507894 1102 56-57 -0.29024655985666925 1102 58-59 -0.1447928723017995 1102 60-61 -0.22896109980528934 1102 62-63 -0.14324654238357937 1102 64-65 -0.28626328807032664 1102 66-67 -0.41878502725474576 1102 68-69 -0.2239142801364693 1102 70-71 -0.7539420397881109 1102 72-73 -0.5328306776880609 1102 74-75 -0.3798503549117669 1102 76-77 -0.6599540247349722 1102 78-79 -0.42684157409744117 1102 80-81 -0.5036674588663388 1102 82-83 -0.8422175585582181 1102 84-85 -0.739853414458107 1102 86-87 -0.4116751363463109 1102 88-89 -0.5143866991929826 1102 90-91 -0.4421217450252044 1102 92-93 -0.35570513070652154 1102 94-95 -0.16438227468461264 1102 96-97 -0.36498872929238146 1102 98-99 -0.5553553645906355 1102 100-101 -0.42560637841897275 1102 102-103 -0.4831414565690011 1102 104-105 -0.36260343398854644 1102 106-107 -0.2584327435859173 1102 108-109 -0.2499858177903569 1102 110-111 -0.21910342876010702 1102 112-113 -0.04144913322857846 1102 114-115 -0.0704686097265288 1102 116-117 -0.14636675787973275 1102 118-119 0.06120151242015126 1102 120-121 0.27308642511460945 1102 122-123 0.18946937169288347 1102 124-125 0.42850256739532355 1103 1 0.18973449814943422 1103 2 -0.23182331500970577 1103 3 0.13996151939093338 1103 4 0.22692343168856155 1103 5 0.22544899986097278 1103 6 0.3683291826775772 1103 7 0.37438155359267 1103 8 0.49731897841926553 1103 9 0.5684282558748706 1103 10-11 0.5808845459683525 1103 12-13 0.6088525428939064 1103 14-15 0.6306175814130555 1103 16-17 0.5606785634525551 1103 18-19 0.5714451525757482 1103 20-21 0.6153065124315873 1103 22-23 0.627821137999689 1103 24-25 0.5901630577628652 1103 26-27 0.6801110340855416 1103 28-29 0.626794997659637 1103 30-31 0.5860122087191115 1103 32-33 0.6415697634916242 1103 34-35 0.5946691064625966 1103 36-37 0.6586697182637948 1103 38-39 0.6508813645263842 1103 40-41 0.6248113299390639 1103 42-43 0.6732082610042909 1103 44-45 0.6523101944781686 1103 46-47 0.7308374820988774 1103 48-49 0.7104398617028949 1103 50-51 0.6327869132723052 1103 52-53 0.6341563569365078 1103 54-55 0.6785219845056147 1103 56-57 0.6985677687108591 1103 58-59 0.8152644230461163 1103 60-61 0.8453389174473145 1103 62-63 0.6715861073384275 1103 64-65 0.6274836996803259 1103 66-67 0.6143150712520686 1103 68-69 0.6265938369363759 1103 70-71 0.5152577453891176 1103 72-73 0.5211946057277324 1103 74-75 0.6740860219296714 1103 76-77 0.6628141280674669 1103 78-79 0.507841311873964 1103 80-81 0.5736421641920515 1103 82-83 0.6277068360700966 1103 84-85 0.6682248400233952 1103 86-87 0.737567557731591 1103 88-89 0.7999395030632108 1103 90-91 0.7595598275220006 1103 92-93 0.6943773757075498 1103 94-95 0.6808177442375971 1103 96-97 0.7616819908091301 1103 98-99 0.8398136274675991 1103 100-101 0.6600359750590954 1103 102-103 0.5633058979386583 1103 104-105 0.6170997098814901 1103 106-107 0.7115159994782552 1103 108-109 0.7269685963723624 1103 110-111 0.5980964454628328 1103 112-113 0.5549730380397122 1103 114-115 0.7327016957559032 1103 116-117 0.7123904701273673 1103 118-119 0.6434186670758528 1103 120-121 0.6481184259160209 1103 122-123 0.6987721437970897 1103 124-125 0.963318882795221 1104 1 0.3920290947445473 1104 2 0.602996177774731 1104 3 0.3237297814085629 1104 4 0.1144832114156884 1104 5 0.39742746603522505 1104 6 0.5448278099093073 1104 7 0.6235720665302935 1104 8 0.5465008295303377 1104 9 0.6612922232014142 1104 10-11 0.6143296956079638 1104 12-13 0.6157919783527959 1104 14-15 0.6998934264660974 1104 16-17 0.6793793157190748 1104 18-19 0.7007727848523331 1104 20-21 0.5496986884399035 1104 22-23 0.6649803738690991 1104 24-25 0.7404792144316872 1104 26-27 0.6631964554440373 1104 28-29 0.8985588867672618 1104 30-31 0.7743150681717879 1104 32-33 0.7319922768912122 1104 34-35 0.9692453331609769 1104 36-37 0.9565885175771456 1104 38-39 1.1091186303289717 1104 40-41 1.0393511244700449 1104 42-43 0.9902193008959372 1104 44-45 0.9709646190966552 1104 46-47 0.9459045981306033 1104 48-49 0.9714720102033496 1104 50-51 0.9043093211550897 1104 52-53 0.8848318481670603 1104 54-55 0.6139951827144543 1104 56-57 0.8722341099028341 1104 58-59 0.9873364631949997 1104 60-61 1.0195838949003218 1104 62-63 0.8678478729861538 1104 64-65 0.8287059359014535 1104 66-67 0.9488837647861423 1104 68-69 0.6778366778054981 1104 70-71 0.336686316817687 1104 72-73 0.2673146323956601 1104 74-75 0.7538501577681096 1104 76-77 0.8615130167288712 1104 78-79 0.8961667445715698 1104 80-81 0.8373484547779597 1104 82-83 0.787504543048378 1104 84-85 0.8576881217532382 1104 86-87 0.8379620645586847 1104 88-89 0.8578699302225026 1104 90-91 0.9055188229601079 1104 92-93 0.8293538312881275 1104 94-95 0.8637960739224653 1104 96-97 0.9018190576521832 1104 98-99 0.879583616600943 1104 100-101 0.8874892405676995 1104 102-103 0.7807677465299321 1104 104-105 0.8532886899382923 1104 106-107 0.8894464528855046 1104 108-109 0.8232043765404526 1104 110-111 0.8988634108312716 1104 112-113 0.9443636129225155 1104 114-115 1.0234754068498653 1104 116-117 1.003612102645441 1104 118-119 0.914776369014529 1104 120-121 0.8170470104685705 1104 122-123 0.8876329036230111 1104 124-125 1.3031292937857657 1105 1 0.17339420652834647 1105 2 -0.15107842856414777 1105 3 0.08177249083069071 1105 4 0.20323851103579216 1105 5 -0.10153957524872226 1105 6 0.3090147290074441 1105 7 0.3701222534250732 1105 8 0.4529021213818538 1105 9 0.5363963661845261 1105 10-11 0.5074962928244204 1105 12-13 0.5322506177132382 1105 14-15 0.6641487482016402 1105 16-17 0.6177794363580915 1105 18-19 0.6329381001498291 1105 20-21 0.6508911369128896 1105 22-23 0.6990873140014671 1105 24-25 0.5412853412594103 1105 26-27 0.5388013318575844 1105 28-29 0.4748818874095164 1105 30-31 0.5715789756087588 1105 32-33 0.71372557825417 1105 34-35 0.8431899356897858 1105 36-37 0.7820957369071238 1105 38-39 0.7623459053018777 1105 40-41 0.5200882615021598 1105 42-43 0.567503223443822 1105 44-45 0.6278878544987165 1105 46-47 0.5570142973892089 1105 48-49 0.603624379567762 1105 50-51 0.45850237437516483 1105 52-53 0.5551403829900678 1105 54-55 0.5307037523013634 1105 56-57 0.6024241486992494 1105 58-59 0.3309388249160108 1105 60-61 0.36910882700853165 1105 62-63 0.623076587919293 1105 64-65 0.3761194458167978 1105 66-67 0.5212170965675185 1105 68-69 0.5456601560634411 1105 70-71 0.5985485291522004 1105 72-73 0.6506009995392183 1105 74-75 0.6470732398623973 1105 76-77 0.6235459123318847 1105 78-79 0.5372939370060728 1105 80-81 0.40182673496829935 1105 82-83 0.542808075386958 1105 84-85 0.5616934589437115 1105 86-87 0.6175063392618156 1105 88-89 0.5938504611428463 1105 90-91 0.4913221006479169 1105 92-93 0.5841572445137793 1105 94-95 0.5461228577258979 1105 96-97 0.5302748522699332 1105 98-99 0.42718678896239837 1105 100-101 0.5705779397381008 1105 102-103 0.15019766505979248 1105 104-105 0.36677050187229554 1105 106-107 0.3330977918778011 1105 108-109 0.37889526516430294 1105 110-111 0.27375962350566496 1105 112-113 0.33149176007108494 1105 114-115 0.33410424423265184 1105 116-117 0.12081224263223334 1105 118-119 0.209967892519348 1105 120-121 0.23153834652249117 1105 122-123 0.35483711103833215 1105 124-125 0.7800329602654195 1106 1 -0.2552512093934851 1106 2 -0.6262309602809957 1106 3 -0.05246188735807422 1106 4 -0.2986298375452705 1106 5 -0.029696019522674533 1106 6 0.1515221547131489 1106 7 0.28316597879194205 1106 8 0.28567897863139535 1106 9 0.30060378527915077 1106 10-11 0.24485469355984435 1106 12-13 0.23560608683989415 1106 14-15 0.24414940930959972 1106 16-17 0.2602641087137698 1106 18-19 0.3086628168859704 1106 20-21 0.32603699222326554 1106 22-23 0.2913371885085425 1106 24-25 0.2807671593533314 1106 26-27 0.39439938109261163 1106 28-29 0.3962665479802254 1106 30-31 0.40481089985885177 1106 32-33 0.3436942749229459 1106 34-35 0.37781700024763865 1106 36-37 0.42524644940120027 1106 38-39 0.3997932551082144 1106 40-41 0.3252072230452754 1106 42-43 0.17090197815682728 1106 44-45 0.11415503513980951 1106 46-47 0.04390182202070747 1106 48-49 0.24694608515312666 1106 50-51 0.3321989072689391 1106 52-53 0.12426990109462821 1106 54-55 0.250983813834047 1106 56-57 -0.17484322295761956 1106 58-59 0.19625545512592169 1106 60-61 0.20070588092332997 1106 62-63 0.09957877284190175 1106 64-65 0.2396557554076466 1106 66-67 0.22457289232853483 1106 68-69 0.2854949352546896 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12744.0 5 10089.0 6 10634.0 7 12883.0 8 14381.0 9 15394.0 10 17167.0 11 18819.0 12 20890.0 13 23652.0 14 26990.0 15 23872.0 16 16464.0 17 14738.0 18 13581.0 19 12858.0 20 12884.0 21 13342.0 22 15110.0 23 18445.0 24 24189.0 25 31428.0 26 39718.0 27 44998.0 28 51262.0 29 61339.0 30 71198.0 31 83328.0 32 101220.0 33 125866.0 34 162104.0 35 222782.0 36 354977.0 37 556549.0 38 213530.0 39 704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30257008572662 19.66744876003748 13.90907707142821 24.120904082807687 2 15.321901615040368 20.637530058138655 39.67479845539184 24.365769871429144 3 19.074487402514016 25.252414471153717 29.2941962075506 26.378901918781672 4 12.623372983199902 17.043914611666832 37.1744847171857 33.158227687947566 5 13.041313873666175 38.71900506637389 34.2540467403813 13.985634319578635 6 31.56093863453309 37.4373800931603 17.233177262415765 13.768504009890844 7 27.989651568852185 32.78451282294608 20.98283649222098 18.24299911598076 8 25.911654727520663 36.181096451549415 19.367242115613042 18.54000670531688 9 26.264383507300554 15.978654108979013 19.867694679551054 37.88926770416938 10-11 24.89864375435707 27.05251623479219 27.26389181925929 20.78494819159145 12-13 25.704545657841056 24.288940043954984 27.9791052426683 22.027409055535657 14-15 22.607063060400233 25.559656433700923 26.004068036550105 25.829212469348743 16-17 22.033680776615657 28.905353369691657 26.522435118360306 22.53853073533238 18-19 21.916046353934025 27.240996625527252 29.208878365551872 21.63407865498685 20-21 22.69323264495426 26.456193107316 29.303309092698115 21.54726515503162 22-23 22.4019520337639 26.314322861064216 29.09398362752682 22.189741477645068 24-25 22.07627601489849 26.533689422374263 29.31521697279979 22.074817589927456 26-27 21.964755118779962 26.828446831853714 29.140925465927886 22.065872583438438 28-29 21.91444918011237 26.709225451888113 29.341526959277274 22.034798408722235 30-31 22.16691226543179 26.552590609998884 29.26790566673939 22.012591457829934 32-33 21.99942674175805 26.605696724610862 29.11239867349443 22.282477860136655 34-35 22.266571305119225 26.602799320335073 29.151134440725134 21.979494933820565 36-37 22.33937587967333 26.422324091585974 29.010911741176628 22.227388287564068 38-39 22.177743501550015 26.732365794761883 28.981879361419878 22.108011342268227 40-41 22.326075043937482 26.53201709507414 28.983862819380484 22.158045041607892 42-43 22.260309800576913 26.5631301611229 28.788822786587314 22.38773725171287 44-45 22.330955906173884 26.72277908128561 28.632771314686494 22.313493697854014 46-47 22.350809931446246 27.046335522526444 28.150479899598135 22.452374646429174 48-49 22.566754055490932 27.013977933835733 27.887905067812873 22.531362942860465 50-51 22.43160031509776 27.13562640653017 27.79191802635706 22.64085525201501 52-53 22.62876628525937 27.224671727984685 27.589258525077327 22.55730346167862 54-55 22.562942704899957 26.99278215759001 27.748187955587657 22.696087181922376 56-57 22.422519872620754 27.28300070496147 27.66015995332669 22.634319469091086 58-59 22.554696265816755 27.035249460864293 27.6247498779774 22.78530439534156 60-61 22.657195849361425 27.0428936395948 27.795829837974818 22.50408067306896 62-63 22.557906277333313 27.08170718949063 27.817900669203162 22.542485863972896 64-65 22.38834268024237 26.9280041691622 27.854456569930697 22.829196580664732 66-67 22.37988120253556 27.1762520189463 27.64743995858851 22.79642681992963 68-69 22.36845150045707 27.055596920817038 27.743604173274143 22.83234740545175 70-71 22.24378098423851 26.996962975840315 27.869587250176664 22.889668789744512 72-73 22.49849976684646 27.09032161965288 27.521820954416636 22.889357659084023 74-75 22.303274944389553 27.31400033719212 27.475265285993945 22.907459432424385 76-77 22.470536898735137 27.107628262642503 27.538369216421323 22.88346562220104 78-79 22.45350249507344 27.196806088204767 27.441393678680587 22.908297738041206 80-81 22.258687217393316 27.285301564775448 27.54075963043072 22.915251587400515 82-83 22.38320641146952 27.333723024485597 27.395054655934214 22.88801590811067 84-85 22.40315766507329 27.14506117023242 27.36586671084722 23.085914453847074 86-87 22.265715695802886 27.10562535901561 27.520401420778164 23.10825752440334 88-89 22.37157794968865 27.078471034668635 27.53220347156943 23.01774754407328 90-91 22.299283252047193 27.290228375782906 27.55231711687606 22.85817125529384 92-93 22.35159092782992 27.185843674792043 27.504838200313937 22.9577271970641 94-95 22.2432948425815 27.053549864716498 27.516142819862736 23.187012472839267 96-97 22.246116596899114 27.04709674596345 27.423423423423422 23.283363233714017 98-99 22.09638002687886 26.95116280923962 27.421008099245263 23.531449064636252 100-101 22.11471989555114 27.09194754766286 27.492731536371686 23.300601020414312 102-103 22.257879092291855 26.9210859860139 27.444096626293575 23.376938295400674 104-105 22.271802189348673 26.82185475098463 27.537260813443332 23.36908224622336 106-107 21.99981370707557 27.01416584620644 27.591829480135786 23.394190966582197 108-109 22.029299568908943 27.03794025240091 27.239941873003048 23.692818305687098 110-111 21.927172267132118 27.034144161645298 27.43667062776044 23.602012943462146 112-113 21.969752655014048 27.034843133754684 27.461714399943688 23.533689811287587 114-115 21.957715787586434 27.123904285319263 27.335123112457786 23.58325681463652 116-117 21.79223316753958 27.053744321379302 27.33156455552846 23.82245795555265 118-119 21.71277817511756 27.020181101379205 27.33026169588767 23.936779027615568 120-121 21.736520460268288 27.036512934714953 27.44032595952263 23.786640645494128 122-123 21.770389406327695 27.189563455369807 27.193569433222198 23.8464777050803 124-125 21.598780124673283 27.10353565332809 27.168246653030327 24.1294375689683 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 358.0 1 287.0 2 389.5 3 581.0 4 436.0 5 322.0 6 652.5 7 1537.5 8 2631.5 9 2998.5 10 2289.5 11 1470.5 12 1274.0 13 1417.0 14 1624.5 15 1790.5 16 1979.5 17 2225.5 18 2543.5 19 2932.5 20 3382.5 21 4047.5 22 4923.5 23 6062.5 24 7480.5 25 9597.0 26 12979.0 27 16938.5 28 20619.5 29 25090.5 30 30939.5 31 36203.0 32 41725.0 33 47573.5 34 54070.0 35 60335.5 36 66307.0 37 72783.0 38 76038.0 39 77915.5 40 80219.5 41 82873.0 42 84558.0 43 86644.5 44 89708.0 45 91095.0 46 91735.0 47 91855.0 48 93807.0 49 94813.5 50 92758.0 51 89750.0 52 87296.0 53 86442.0 54 83584.5 55 76969.0 56 70106.0 57 64586.5 58 60009.5 59 55021.0 60 48147.5 61 39600.5 62 30853.5 63 23289.0 64 17176.5 65 12800.0 66 9829.0 67 7878.5 68 5945.0 69 4197.0 70 3100.0 71 2380.0 72 1937.0 73 1740.5 74 1341.5 75 934.0 76 650.0 77 399.5 78 240.0 79 135.5 80 85.5 81 66.5 82 49.0 83 33.0 84 18.0 85 12.5 86 9.0 87 5.5 88 4.5 89 1.5 90 1.5 91 1.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0980061580535977 2 0.0 3 0.0 4 0.09847285404432912 5 0.0 6 0.0 7 0.002177914623413282 8 0.006728200533044604 9 0.0036168939281684864 10-11 5.639243221337962E-4 12-13 0.0 14-15 7.778266512190294E-5 16-17 3.1113066048761177E-4 18-19 0.001964012294328049 20-21 0.0013028596407918742 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 5.8336998841427204E-5 52-53 0.0 54-55 0.0 56-57 0.007953277508714576 58-59 8.556093163409324E-4 60-61 0.0 62-63 0.0 64-65 2.7223932792666024E-4 66-67 0.0 68-69 1.9445666280475736E-5 70-71 0.0 72-73 0.0 74-75 0.0012639683082309229 76-77 0.0 78-79 0.0 80-81 1.3611966396333012E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0014778706373161558 90-91 1.9445666280475736E-5 92-93 0.0024501539513399426 94-95 0.0 96-97 0.008925560822738363 98-99 0.003947470254936573 100-101 0.002197360289693758 102-103 0.0 104-105 0.0 106-107 0.0018473382966451948 108-109 1.9445666280475736E-5 110-111 2.91684994207136E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010695116454261654 122-123 0.004258600915424186 124-125 0.08369414767116756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2571267.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.84499516233 #Duplication Level Percentage of deduplicated Percentage of total 1 74.77910584031167 38.76922380534321 2 12.83785757833579 13.31157328087001 3 4.895376122004288 7.614022540892942 4 2.372311137145935 4.919698377154902 5 1.3642775928538382 3.5365482600791225 6 0.8337981767878443 2.5936957465155217 7 0.5417725388199963 1.9661736257944165 8 0.3938988004405749 1.6337345122631357 9 0.30017384530047936 1.4006260401715211 >10 1.5137782908751989 14.303360931828358 >50 0.11177734135219798 3.9573209682370187 >100 0.054365990977748385 4.839996861309466 >500 0.0011300585957808688 0.36219028919063695 >1k 2.2601171915484115E-4 0.2704026067135 >5k 1.5067447943656077E-4 0.5214321536364618 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7362 0.28631799031372474 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5988 0.2328812993749774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3944 0.1533874156203926 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.889133256095147E-5 0.0 14-15 1.9445666280475736E-5 0.0 0.0 5.8336998841427204E-5 0.0 16-17 3.889133256095147E-5 0.0 0.0 1.1667399768285441E-4 0.0 18-19 5.8336998841427204E-5 0.0 0.0 1.7501099652428162E-4 0.0 20-21 7.778266512190294E-5 0.0 0.0 2.91684994207136E-4 0.0 22-23 1.5556533024380589E-4 0.0 0.0 5.055873232923691E-4 0.0 24-25 1.9445666280475734E-4 0.0 0.0 7.583809849385536E-4 0.0 26-27 1.9445666280475734E-4 0.0 0.0 9.139463151823595E-4 0.0 28-29 3.1113066048761177E-4 0.0 0.0 0.0014973163035966316 0.0 30-31 3.1113066048761177E-4 0.0 0.0 0.003441882931644205 0.0 32-33 3.1113066048761177E-4 0.0 0.0 0.007311570521458876 0.0 34-35 3.5002199304856325E-4 0.0 0.0 0.012698020081150655 0.0 36-37 4.2780465817046615E-4 0.0 0.0 0.021331895909681882 0.0 38-39 4.2780465817046615E-4 0.0 0.0 0.03749124458875722 0.0 40-41 4.2780465817046615E-4 0.0 0.0 0.05775362885301293 0.0 42-43 4.4725032445094186E-4 0.0 0.0 0.07577976149501393 0.0 44-45 5.055873232923691E-4 0.0 0.0 0.09654773308256202 0.0 46-47 6.222613209752234E-4 0.0 0.0 0.12519119951370278 0.0 48-49 7.389353186580779E-4 0.0 0.0 0.15583756957173253 0.0 50-51 7.389353186580779E-4 0.0 0.0 0.19194817185457597 0.0 52-53 7.389353186580779E-4 0.0 0.0 0.22860325279327273 0.0 54-55 7.389353186580779E-4 0.0 0.0 0.2669501066983709 0.0 56-57 9.139463151823595E-4 0.0 0.0 0.3123751831295622 0.0 58-59 9.333919814628353E-4 0.0 0.0 0.3671147337091014 0.0 60-61 9.333919814628353E-4 0.0 0.0 0.4300214641264404 0.0 62-63 9.333919814628353E-4 0.0 0.0 0.4939588148566446 0.0 64-65 9.333919814628353E-4 0.0 0.0 0.5590629055636773 0.0 66-67 9.333919814628353E-4 0.0 0.0 0.635853841705276 0.0 68-69 9.333919814628353E-4 0.0 0.0 0.7233787856336973 0.0 70-71 9.333919814628353E-4 0.0 0.0 0.8297271345216191 0.0 72-73 9.333919814628353E-4 0.0 0.0 0.9413647046378304 0.0 74-75 9.333919814628353E-4 0.0 0.0 1.0465074222163626 0.0 76-77 9.333919814628353E-4 0.0 0.0 1.1690151197833596 0.0 78-79 9.722833140237867E-4 0.0 0.0 1.3108518096331498 0.0 80-81 0.0011472943105480683 0.0 0.0 1.4669227272002479 0.0 82-83 0.0012056313093894956 0.0 0.0 1.6412920167372738 0.0 84-85 0.0012445226419504469 0.0 0.0 1.815952991268507 0.0 86-87 0.0012834139745113984 0.0 0.0 1.9998312116166854 0.0 88-89 0.0012834139745113984 0.0 0.0 2.2043801752210097 0.0 90-91 0.00132230530707235 0.0 0.0 2.448345504375858 0.0 92-93 0.0013611966396333015 0.0 0.0 2.7039198963001505 0.0 94-95 0.0013611966396333015 0.0 0.0 2.9636751064747457 0.0 96-97 0.0013611966396333015 0.0 0.0 3.2283306245520205 0.0 98-99 0.0013611966396333015 0.0 0.0 3.5206962170789735 0.0 100-101 0.001400087972194253 0.0 0.0 3.8636205419351626 0.0 102-103 0.00145842497103568 0.0 0.0 4.234060484578226 0.0 104-105 0.0017112186326818646 0.0 0.0 4.6161872726558535 0.0 106-107 0.0017112186326818646 0.0 0.0 4.996641733433362 0.0 108-109 0.0017306642989623404 0.0 0.0 5.393508336551591 0.0 110-111 0.0018473382966451948 0.0 0.0 5.836441723088267 0.0 112-113 0.0018667839629256705 0.0 0.0 6.324080696403757 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5140 0.0 62.495636 1 GTATCAA 9100 0.0 50.10073 1 ATCAACG 11260 0.0 39.98243 3 TCAACGC 11410 0.0 39.352425 4 CAACGCA 11555 0.0 38.620132 5 TATCAAC 11955 0.0 37.757687 2 AACGCAG 12000 0.0 37.287136 6 GTACATG 11495 0.0 33.285114 1 TACATGG 11705 0.0 31.899353 2 ACGCAGA 13875 0.0 31.862383 7 CGCAGAG 14120 0.0 31.140974 8 ACATGGG 11770 0.0 30.863071 3 GCAGAGT 15375 0.0 28.094326 9 GAGTACT 9160 0.0 26.533447 12-13 CATGGGA 6560 0.0 25.236359 4 GTACTTT 10010 0.0 24.072323 14-15 AGTACTT 9605 0.0 23.972357 12-13 CAGAGTA 15500 0.0 23.951523 10-11 AGAGTAC 14710 0.0 23.377356 10-11 AGTACAT 8660 0.0 22.348646 2 >>END_MODULE