FastQCFastQC Report
Thu 26 May 2016
SRR1364522_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364522_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321092
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.11149924259788065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44650.054.231221
GGTATCA29550.045.7030141
ATCAACG54100.043.5495573
CAACGCA55200.043.005075
TCAACGC55350.042.8885234
AACGCAG58050.041.0960356
TATCAAC59600.039.430052
GTACATG79200.035.230931
ACGCAGA69450.033.7506187
TACATGG81200.033.410682
CGCAGAG70250.032.3521278
ACATGGG83400.031.3886383
GCAGAGT80250.027.4322389
CATGGGG47650.025.8459974
AGAGTAC71450.023.7362810-11
CAGAGTA76600.022.29580710-11
CATGGGA45050.022.1870884
GTGGTAT18150.021.6343461
GAGTACA55600.021.6148911
GAGTACT40750.021.39571812-13
Produced by FastQC (version 0.11.5)