##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364522_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2321092 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.293263257122078 31.0 27.0 31.0 10.0 34.0 2 27.334742009364557 31.0 27.0 34.0 10.0 34.0 3 27.428668488797513 31.0 27.0 34.0 10.0 34.0 4 30.655918851988634 35.0 31.0 37.0 10.0 37.0 5 30.77072860532887 35.0 31.0 37.0 10.0 37.0 6 30.72374511652274 35.0 31.0 37.0 8.0 37.0 7 30.520750146913606 35.0 31.0 37.0 8.0 37.0 8 30.451727031931522 35.0 31.0 37.0 8.0 37.0 9 31.452517608091362 37.0 30.0 39.0 7.0 39.0 10-11 31.35522482521158 37.0 30.0 39.0 2.0 39.0 12-13 31.262077074066863 37.0 30.0 39.0 2.0 39.0 14-15 31.896509272359737 38.0 30.0 40.0 2.0 41.0 16-17 31.98153800021714 38.0 30.5 40.0 2.0 41.0 18-19 31.75723797247158 38.0 30.0 40.0 2.0 41.0 20-21 31.456799428889504 38.0 29.5 40.0 2.0 41.0 22-23 31.329035212736073 37.5 29.0 40.0 2.0 41.0 24-25 31.139422737228855 37.0 28.0 40.0 2.0 41.0 26-27 31.018396082533563 37.0 28.0 40.0 2.0 41.0 28-29 31.057677808548732 37.0 28.5 40.0 2.0 41.0 30-31 30.994501295080074 37.0 28.5 40.0 2.0 41.0 32-33 30.967212630951295 37.0 29.0 40.0 2.0 41.0 34-35 30.49327794848287 37.0 27.0 40.0 2.0 41.0 36-37 30.36206169337536 37.0 27.0 40.0 2.0 41.0 38-39 30.1538954509343 36.0 27.0 40.0 2.0 41.0 40-41 30.363057345421897 37.0 27.5 40.0 2.0 41.0 42-43 30.238606655832687 36.0 27.5 40.0 2.0 41.0 44-45 30.082551876444363 36.0 27.0 40.0 2.0 41.0 46-47 30.05371738819487 36.0 27.0 40.0 2.0 41.0 48-49 29.92450880878483 36.0 27.0 40.0 2.0 41.0 50-51 29.767265795582425 36.0 27.0 40.0 2.0 41.0 52-53 29.70818239862961 36.0 27.0 40.0 2.0 41.0 54-55 29.730588662577787 36.0 27.0 40.0 2.0 41.0 56-57 29.597809996329314 36.0 27.0 40.0 2.0 41.0 58-59 29.397461841236797 35.0 26.5 40.0 2.0 41.0 60-61 29.199974193181482 35.0 26.0 39.0 2.0 41.0 62-63 28.92270211607295 35.0 26.0 39.0 2.0 41.0 64-65 28.64584751487662 35.0 26.0 39.0 2.0 40.5 66-67 28.404022977115943 34.0 26.0 38.5 2.0 40.0 68-69 28.36927015387585 34.0 26.0 38.0 2.0 40.0 70-71 28.15261394205831 34.0 26.0 37.5 2.0 40.0 72-73 27.87833636064404 34.0 26.0 37.0 2.0 39.5 74-75 27.584497512377794 34.0 25.5 36.5 2.0 39.0 76-77 27.29190247521425 34.0 25.0 36.0 2.0 39.0 78-79 26.996329313960842 34.0 24.5 36.0 2.0 38.5 80-81 26.70636364263028 34.0 24.0 35.0 2.0 37.0 82-83 26.469269852293664 33.5 23.5 35.0 2.0 37.0 84-85 26.236722413415755 33.0 23.0 35.0 2.0 36.5 86-87 26.04377831641314 33.0 22.5 35.0 2.0 36.0 88-89 25.85456349856016 33.0 21.0 35.0 2.0 36.0 90-91 25.72086242165326 33.0 20.0 35.0 2.0 35.5 92-93 25.594231939104525 33.0 20.0 35.0 2.0 35.0 94-95 25.473800478395514 33.0 20.0 35.0 2.0 35.0 96-97 25.357617018196606 33.0 19.0 35.0 2.0 35.0 98-99 25.267083984607247 33.0 18.5 35.0 2.0 35.0 100-101 25.17362000299859 33.0 18.0 35.0 2.0 35.0 102-103 24.07616371949065 30.5 15.5 34.0 2.0 35.0 104-105 24.902962915731045 32.5 17.0 35.0 2.0 35.0 106-107 25.042643074897505 33.0 17.0 35.0 2.0 35.0 108-109 24.993499395973963 33.0 14.0 35.0 2.0 35.0 110-111 24.90508153058991 33.0 10.0 35.0 2.0 35.0 112-113 24.813276681837685 33.0 7.0 35.0 2.0 35.0 114-115 24.684292781156458 33.0 2.0 35.0 2.0 35.0 116-117 24.572294635456068 33.0 2.0 35.0 2.0 35.0 118-119 24.418845741573364 33.0 2.0 35.0 2.0 35.0 120-121 24.236218124917066 33.0 2.0 35.0 2.0 35.0 122-123 23.985458353223397 33.0 2.0 35.0 2.0 35.0 124-125 22.608581650361124 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6480941245237517 1101 2 0.5827019231856454 1101 3 0.6032824444595626 1101 4 0.6267871296848995 1101 5 0.6215917014385539 1101 6 0.6060221452788106 1101 7 0.7110914563462636 1101 8 0.6660873682096415 1101 9 0.8332139121489668 1101 10-11 0.9886826215265074 1101 12-13 0.734476588977536 1101 14-15 1.0626384165716516 1101 16-17 0.8162255035455388 1101 18-19 1.1111523867251911 1101 20-21 1.5826803740554354 1101 22-23 1.4318210413445982 1101 24-25 1.5947020474455869 1101 26-27 1.6993635521609498 1101 28-29 1.3973754916510401 1101 30-31 1.541459053746955 1101 32-33 1.422448443211728 1101 34-35 1.789163485539838 1101 36-37 1.5861152012274609 1101 38-39 1.4986250768655296 1101 40-41 1.2521370123852797 1101 42-43 1.165142867650534 1101 44-45 1.140394741088496 1101 46-47 1.0667651729321825 1101 48-49 1.004718086276192 1101 50-51 1.0102926468925517 1101 52-53 0.9032624786277665 1101 54-55 0.765899906794143 1101 56-57 0.7633509617949166 1101 58-59 0.8115401979283696 1101 60-61 0.7577622867435849 1101 62-63 0.6777702006486699 1101 64-65 0.8411078572387432 1101 66-67 0.7101962109595732 1101 68-69 0.5544138175504933 1101 70-71 0.4868060758905379 1101 72-73 0.5181415151797957 1101 74-75 0.453375198315058 1101 76-77 0.5923109141779719 1101 78-79 0.6212559224606906 1101 80-81 0.6724705010938727 1101 82-83 0.6315500064557042 1101 84-85 0.6788533667976893 1101 86-87 0.765042793071526 1101 88-89 0.6801572631427248 1101 90-91 0.6479190382641313 1101 92-93 0.5783509161023019 1101 94-95 0.6111942754615001 1101 96-97 0.4481214870237693 1101 98-99 0.6063137507287522 1101 100-101 0.7309432353557064 1101 102-103 0.7391713565570726 1101 104-105 0.8288009313082014 1101 106-107 0.7120777443492301 1101 108-109 0.7863030077592335 1101 110-111 0.5393472326165778 1101 112-113 0.5592154208097142 1101 114-115 0.6217121134617649 1101 116-117 0.6902026250962159 1101 118-119 0.6706393356978424 1101 120-121 0.7795127112045073 1101 122-123 0.8064644431477213 1101 124-125 1.0182324500870088 1102 1 -0.21730410635034403 1102 2 -0.056060613259383274 1102 3 0.24593942963599957 1102 4 0.2995193557247262 1102 5 0.2803409454036334 1102 6 -0.5360227258858039 1102 7 -1.1728280598315841 1102 8 -0.23839219418369595 1102 9 0.17844788977787118 1102 10-11 0.14631018687380504 1102 12-13 0.2776709933392709 1102 14-15 0.44375932638293847 1102 16-17 0.29497813762899483 1102 18-19 0.4005693801625334 1102 20-21 0.6851525224072788 1102 22-23 0.45152091202096045 1102 24-25 0.7998878733338657 1102 26-27 0.25748854703138946 1102 28-29 0.7300759554306211 1102 30-31 0.5833930928301285 1102 32-33 0.4111958966449194 1102 34-35 0.7851976416524451 1102 36-37 0.8355434118697218 1102 38-39 0.8433449674973623 1102 40-41 0.6218165658008381 1102 42-43 0.41723284045421494 1102 44-45 0.5478417876572301 1102 46-47 0.2838431287377041 1102 48-49 0.031990700869815925 1102 50-51 0.42145709895545735 1102 52-53 0.2526637977925006 1102 54-55 0.1010450579617661 1102 56-57 0.15377158269241775 1102 58-59 0.29036221721620237 1102 60-61 0.20289789399100044 1102 62-63 0.12046819721265578 1102 64-65 0.2593551693890852 1102 66-67 0.22620518237856047 1102 68-69 0.08045634176161798 1102 70-71 -0.009528023625183124 1102 72-73 0.1188945267991599 1102 74-75 -0.2524969422984782 1102 76-77 -0.03503723272201853 1102 78-79 -0.1356824723551071 1102 80-81 -0.40044183335939465 1102 82-83 -0.19783261345926562 1102 84-85 -0.6280454319714828 1102 86-87 -0.13038403103911733 1102 88-89 -0.011160608526225246 1102 90-91 -0.5610498096634338 1102 92-93 -0.03927601611023945 1102 94-95 -0.09826642437349875 1102 96-97 0.018814667408719288 1102 98-99 0.12546084283964376 1102 100-101 -0.013635799542576876 1102 102-103 -0.06194409958552782 1102 104-105 0.22949927224389555 1102 106-107 0.08462173004641471 1102 108-109 -0.3596050741389263 1102 110-111 -0.10429039957333686 1102 112-113 -0.3822829696377852 1102 114-115 -0.48504587982265335 1102 116-117 -0.5765355818896047 1102 118-119 -0.2610434560847352 1102 120-121 0.15787751675089723 1102 122-123 -0.07932298805540228 1102 124-125 0.2499497209144188 1103 1 0.900505850382352 1103 2 0.8653524056771396 1103 3 0.7751927804135903 1103 4 0.9149855246207004 1103 5 0.9005540350084971 1103 6 1.0032213957155847 1103 7 1.0990439963647525 1103 8 1.0899967995632487 1103 9 1.401511894421791 1103 10-11 1.417278936596695 1103 12-13 1.307651065003519 1103 14-15 1.5906012837322763 1103 16-17 1.5266609002154254 1103 18-19 1.490558452490486 1103 20-21 1.821476595413948 1103 22-23 1.782047606031817 1103 24-25 1.8434583844446877 1103 26-27 1.843860300704467 1103 28-29 1.8878643433125006 1103 30-31 1.7936857218356579 1103 32-33 1.757110604868572 1103 34-35 2.053937541911857 1103 36-37 1.9681085396354945 1103 38-39 1.9283367785645993 1103 40-41 1.7998081865546816 1103 42-43 1.754396884456856 1103 44-45 1.5321760625529883 1103 46-47 1.6331625025725423 1103 48-49 1.5360543862783693 1103 50-51 1.5295396710920777 1103 52-53 1.51066378142146 1103 54-55 1.4291930051351365 1103 56-57 1.3203854982164636 1103 58-59 1.298107597000552 1103 60-61 1.1111577167880924 1103 62-63 1.1060206653863247 1103 64-65 1.2206323632283862 1103 66-67 1.2396360041435948 1103 68-69 1.3193903684996258 1103 70-71 1.324761111526655 1103 72-73 1.3755207069638686 1103 74-75 1.3049111087082395 1103 76-77 1.2438846896751414 1103 78-79 1.3276445979169154 1103 80-81 1.316485928725676 1103 82-83 1.394524291676671 1103 84-85 1.1896337439920046 1103 86-87 1.173908405606646 1103 88-89 1.189556068042652 1103 90-91 1.2718112929400363 1103 92-93 1.2765471779759956 1103 94-95 1.2382492580637106 1103 96-97 1.3827218354022364 1103 98-99 1.2828706503229839 1103 100-101 1.252630111918723 1103 102-103 1.1629723057697134 1103 104-105 1.2488975954396864 1103 106-107 1.2646969072459164 1103 108-109 1.3687322826059116 1103 110-111 1.3725682767049534 1103 112-113 1.4824609121960144 1103 114-115 1.5091625222065375 1103 116-117 1.6322338578727944 1103 118-119 1.6182570126046194 1103 120-121 1.4357458942371686 1103 122-123 1.5425765749059295 1103 124-125 1.6576983749076213 1104 1 0.5696866835748935 1104 2 0.6090044845145322 1104 3 0.527101715126971 1104 4 0.5513607959709006 1104 5 0.5712298842828254 1104 6 0.5106161469021941 1104 7 0.6605390443232118 1104 8 0.6058132090382706 1104 9 1.0130289372863537 1104 10-11 0.8395626453841345 1104 12-13 0.8372569258490756 1104 14-15 0.9635810197296522 1104 16-17 1.0264721106046721 1104 18-19 1.2784746423352153 1104 20-21 1.5191907190125775 1104 22-23 1.269276455060691 1104 24-25 1.7706240060876794 1104 26-27 1.757719004152566 1104 28-29 1.5728968097065206 1104 30-31 1.4709185235723012 1104 32-33 1.481434580272527 1104 34-35 1.7843150573800202 1104 36-37 1.8094963385191676 1104 38-39 1.802458655845772 1104 40-41 1.4735191734884303 1104 42-43 1.5117790443566328 1104 44-45 1.4761043527837394 1104 46-47 1.3521426720927074 1104 48-49 1.2880022902275705 1104 50-51 1.1061239418653095 1104 52-53 1.2011312209912504 1104 54-55 1.1398277813130626 1104 56-57 1.2059887164856242 1104 58-59 1.226516727755488 1104 60-61 1.149033981096732 1104 62-63 1.1172966408922846 1104 64-65 1.2435827726842845 1104 66-67 1.2301886341287052 1104 68-69 1.2143876430438674 1104 70-71 1.151808709778539 1104 72-73 1.027969791943896 1104 74-75 1.1340542796086588 1104 76-77 1.1763926030598384 1104 78-79 1.2591273574169648 1104 80-81 1.367337354728754 1104 82-83 1.2734310173364385 1104 84-85 1.203432120831689 1104 86-87 1.1961627081609265 1104 88-89 1.1827862590795704 1104 90-91 1.1818102888247601 1104 92-93 1.2771547474113625 1104 94-95 1.2813162405219778 1104 96-97 1.0480747580152325 1104 98-99 1.2310442761991922 1104 100-101 1.3029181973737138 1104 102-103 1.2562760522353855 1104 104-105 1.3992460075030948 1104 106-107 1.4142973331183022 1104 108-109 1.4434185364271315 1104 110-111 1.4129121941571157 1104 112-113 1.437809738656206 1104 114-115 1.4201084944292006 1104 116-117 1.4120421356078943 1104 118-119 1.456880147420641 1104 120-121 1.5003533011014873 1104 122-123 1.5029555407628195 1104 124-125 1.7376334421195914 1105 1 -0.04849089003285556 1105 2 0.1937972296543542 1105 3 0.3114751875076145 1105 4 0.37422541912678753 1105 5 0.34873392659866553 1105 6 0.24064647993888144 1105 7 0.2443887391126971 1105 8 0.094652186990416 1105 9 -0.018334733261770708 1105 10-11 0.3997780318796629 1105 12-13 0.3010887749108839 1105 14-15 0.6132498005170604 1105 16-17 0.4338587377464762 1105 18-19 0.6510793351818869 1105 20-21 1.0235007207673803 1105 22-23 1.1642586563366848 1105 24-25 1.2164050347787168 1105 26-27 0.9880442068950792 1105 28-29 0.8389686187838663 1105 30-31 0.7998454453957926 1105 32-33 0.8139156064567103 1105 34-35 1.4475325684415452 1105 36-37 1.4332360917185483 1105 38-39 1.457209943746399 1105 40-41 1.0566942872373168 1105 42-43 0.6259103068126066 1105 44-45 0.8548061270157028 1105 46-47 0.8656541127866042 1105 48-49 0.8462854340838781 1105 50-51 0.7886594139701977 1105 52-53 0.7232073575400548 1105 54-55 0.47220790628015763 1105 56-57 0.5885940673837062 1105 58-59 0.6360905141206636 1105 60-61 0.6301753242736794 1105 62-63 0.5621781759695601 1105 64-65 0.6460963724135418 1105 66-67 0.47868404657026886 1105 68-69 0.3231605651681839 1105 70-71 0.49809447957334996 1105 72-73 0.578160235157192 1105 74-75 0.6338379060360033 1105 76-77 0.6234649009753781 1105 78-79 0.7104326494037778 1105 80-81 0.5762035008036364 1105 82-83 0.5472079817765945 1105 84-85 0.4972178436228276 1105 86-87 0.5546115399629841 1105 88-89 0.679062001424235 1105 90-91 0.6560146733273342 1105 92-93 0.5602571621467014 1105 94-95 0.7252408591655168 1105 96-97 0.7154961208710517 1105 98-99 0.4426059262161175 1105 100-101 0.5355904501657314 1105 102-103 0.49637794052959094 1105 104-105 0.6126602289342671 1105 106-107 0.5850362151829529 1105 108-109 0.6541927965893208 1105 110-111 0.7152155307911485 1105 112-113 0.6416963156607522 1105 114-115 0.6617126200982213 1105 116-117 0.7112177695307587 1105 118-119 0.7750742506532404 1105 120-121 0.6078709118108065 1105 122-123 0.737294622123045 1105 124-125 0.8861459325621794 1106 1 0.20596366013634082 1106 2 0.5057026089539676 1106 3 0.32441973495409826 1106 4 0.38534947752246396 1106 5 0.28611610450840175 1106 6 0.12345774418449551 1106 7 0.08360534376205209 1106 8 0.35308400252024086 1106 9 0.5696299103871816 1106 10-11 0.6978550359896616 1106 12-13 0.5726326332521054 1106 14-15 0.8383911221989209 1106 16-17 0.6574962476325084 1106 18-19 0.7055079547672527 1106 20-21 1.035526272116421 1106 22-23 0.9035088086788718 1106 24-25 1.2668363738340602 1106 26-27 1.5494079611250093 1106 28-29 1.287221869830816 1106 30-31 1.0543587445439755 1106 32-33 0.9850718025697134 1106 34-35 1.4405159955571563 1106 36-37 1.374133727497572 1106 38-39 1.4485055329540906 1106 40-41 1.1916128165842572 1106 42-43 1.1511896278202691 1106 44-45 1.0267151203904383 1106 46-47 0.6533165841008959 1106 48-49 0.7132973556697912 1106 50-51 0.8405638532861452 1106 52-53 0.8940389351567717 1106 54-55 0.6841185527296112 1106 56-57 0.7594275087611386 1106 58-59 0.6533631191071159 1106 60-61 0.7726880302597543 1106 62-63 0.7219978449656566 1106 64-65 0.6001365194399533 1106 66-67 0.4756953847180476 1106 68-69 0.5507019244493883 1106 70-71 0.6536517278197955 1106 72-73 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92-93 -2.639732836197851 2216 94-95 -2.7539125869221124 2216 96-97 -2.6889357621072847 2216 98-99 -2.811476358667818 2216 100-101 -2.8018585069030237 2216 102-103 -2.701508261324509 2216 104-105 -2.8400601063856037 2216 106-107 -2.8220616262988543 2216 108-109 -2.8107703697278623 2216 110-111 -2.871561475977785 2216 112-113 -2.8342060175990014 2216 114-115 -2.9227823997671365 2216 116-117 -3.0420459890683667 2216 118-119 -3.064573920998157 2216 120-121 -3.1453784765937094 2216 122-123 -3.0711150456840386 2216 124-125 -3.095461942764313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 214499.0 3 32643.0 4 19780.0 5 15448.0 6 14123.0 7 14819.0 8 15633.0 9 15761.0 10 16344.0 11 16995.0 12 18257.0 13 19082.0 14 20158.0 15 18041.0 16 14688.0 17 13388.0 18 12316.0 19 11775.0 20 11566.0 21 12776.0 22 14955.0 23 19966.0 24 26369.0 25 33467.0 26 40417.0 27 45786.0 28 52244.0 29 60520.0 30 68803.0 31 78467.0 32 94116.0 33 117300.0 34 149249.0 35 199914.0 36 299888.0 37 372837.0 38 118394.0 39 308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46200056790492 18.378920127489916 12.972447993395411 24.186631311209744 2 14.987182193805229 19.489442005351165 41.53779206286972 23.985583737973883 3 19.545180359512607 24.860787963393825 28.212433000706618 27.381598676386947 4 12.327013566608743 16.187725637990813 37.50441096824146 33.980849827158984 5 13.628253073229669 37.87036785683354 34.35148810716457 14.149890962772222 6 32.07133080260724 36.82189228860962 17.508238646522535 13.5985382622606 7 28.872822532346948 31.917334861456396 20.706484038725854 18.503358567470798 8 25.833745495654632 36.41910790265961 19.38173928478492 18.36540731690084 9 26.205280745322344 16.76828795417219 19.39177160543826 37.6346596950672 10-11 24.988173669979474 26.872136046309237 27.370909899306017 20.76878038440527 12-13 25.721893223438887 23.906071266234814 27.672225297983854 22.699810212342445 14-15 22.611372336776572 25.537104577788273 25.248288866558312 26.60323421887685 16-17 22.617296602642366 28.91229651860589 25.729727493416064 22.740679385335678 18-19 22.588948184327336 27.590460490883505 27.703991196738585 22.116600128050575 20-21 23.12934813308053 26.86456585399903 27.971229208725685 22.03485680419476 22-23 22.93906725103052 26.63482034473733 27.691565320324813 22.734547083907337 24-25 22.642182583582795 26.76547481447889 27.889384282114893 22.702958319823423 26-27 22.513643094619486 27.05660131009043 27.629170680397703 22.800584914892383 28-29 22.518848984093612 27.090815482447827 27.68624950454099 22.704086028917573 30-31 22.74400979380322 26.967447409254262 27.50756712613356 22.780975670808957 32-33 22.565516141923545 27.028121664140702 27.441151537282455 22.965210656653298 34-35 22.73209453069223 26.84171249305764 27.815280040432643 22.61091293581749 36-37 22.831897224039082 26.64896197692746 27.642324764935665 22.876816034097793 38-39 22.569462994142413 26.917093333655025 27.81783315784122 22.695610514361345 40-41 22.942078393196734 26.887443531292167 27.386466816028904 22.784011259482195 42-43 22.69069374948565 26.881214504319694 27.326282562526906 23.101809183667754 44-45 22.846625201149557 26.988049201035796 27.247063254196373 22.918262343618274 46-47 22.969285732135116 27.198012413582727 26.86979266748987 22.96290918679228 48-49 22.96469818639704 27.116026332590188 26.896681449822275 23.022594031190497 50-51 22.909306726784887 27.31489789345676 26.840960670750423 22.93483470900793 52-53 23.069378551130242 27.214647243624984 26.663311923870314 23.052662281374456 54-55 23.086989579313837 27.028200940182156 26.78782300582944 23.09698647467457 56-57 22.81359065726867 27.334761889476976 26.81483322967792 23.03681422357643 58-59 22.975392332596105 27.08680916752097 26.822514724252944 23.11528377562998 60-61 23.023221045429594 27.095930285161813 26.89231098001808 22.988537689390512 62-63 22.876127522306966 27.170101350150254 26.99591621617487 22.957854911367907 64-65 22.822716326861702 27.06446301005486 26.89096070893562 23.22185995414782 66-67 22.778905980556615 27.313563084582754 26.732855201306826 23.17467573355381 68-69 22.789799467259837 27.161130091718093 26.99402127039332 23.05504917062875 70-71 22.788953860099923 26.98590883005289 26.989420205184945 23.235717104662246 72-73 22.90796561071233 27.1294562009687 26.77531702974657 23.187261158572394 74-75 22.8417701668008 27.296548348794445 26.712642152917677 23.149039331487074 76-77 22.934717301185135 27.158186086400903 26.789157673798268 23.11793893861569 78-79 22.82604879005027 27.180963069794394 26.828151270913057 23.16483686924228 80-81 22.8262635097145 27.262620017535433 26.740822441905802 23.170294030844264 82-83 23.02999321860979 27.26758659197659 26.655408869575858 23.04701131983776 84-85 22.99191501241657 27.15079798646499 26.732977408909253 23.124309592209183 86-87 22.87306783537242 27.147203843906777 26.939036748396795 23.04069157232401 88-89 22.939517379163274 27.112266018650644 26.770864012509886 23.177352589676197 90-91 22.85681720441228 27.342785142208054 26.78819053922798 23.012207114151686 92-93 22.961717007580596 27.229138926187364 26.792730606725616 23.01641345950642 94-95 22.887955775963263 27.111656384612598 26.799639567895166 23.20074827152897 96-97 23.018012301788303 27.04294939565079 26.831527894689554 23.107510407871352 98-99 22.706617129264917 27.145164879455614 26.73625943501598 23.41195855626349 100-101 22.697381389556192 27.07541212883845 26.922734715939654 23.3044717656657 102-103 22.697075342123448 26.883251504033446 27.007094074685533 23.41257907915757 104-105 22.72152789042404 26.98783937400921 26.98831329612183 23.302319439444926 106-107 22.654509170683454 26.96911626079449 26.970430297463437 23.405944271058623 108-109 22.70687676317871 26.96405398838133 26.742843454718727 23.58622579372123 110-111 22.58103777641251 27.084160894205194 26.888906792447305 23.445894536934993 112-113 22.706833680009236 27.021031480010272 27.02006210869711 23.25207273128338 114-115 22.673185724650295 27.21288083367656 26.78344934194767 23.330484099725474 116-117 22.432566223139798 27.149096201270783 26.950525873166598 23.467811702422825 118-119 22.573879019013464 27.13438329889552 26.855462859722923 23.43627482236809 120-121 22.539352714014075 27.208406045173145 26.88715632279038 23.365084918022404 122-123 22.60212003660346 27.170659327592357 26.846092270362398 23.381128365441782 124-125 22.646120405834473 27.173620759198673 26.67789818974106 23.502360645225792 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 387.0 1 450.5 2 568.5 3 564.0 4 467.5 5 390.0 6 356.0 7 428.0 8 650.0 9 843.0 10 817.0 11 802.0 12 880.0 13 956.0 14 1087.5 15 1233.0 16 1324.5 17 1459.5 18 1682.0 19 1906.5 20 2208.0 21 2711.5 22 3433.5 23 4495.5 24 5921.5 25 7697.0 26 10081.5 27 13101.5 28 16306.5 29 20089.0 30 24670.0 31 29524.0 32 34612.5 33 39644.5 34 44550.0 35 48841.0 36 54333.0 37 60272.0 38 64066.5 39 67005.5 40 69199.5 41 71573.5 42 74398.5 43 77219.0 44 80563.0 45 83890.0 46 85555.0 47 85384.0 48 86300.0 49 86882.0 50 84920.0 51 83008.5 52 82321.5 53 82004.5 54 80991.5 55 76932.0 56 70747.5 57 64444.0 58 59196.5 59 53281.5 60 45298.0 61 37335.0 62 30114.0 63 23712.5 64 18031.5 65 13532.0 66 10350.0 67 8098.5 68 6109.5 69 4582.0 70 3439.0 71 2624.5 72 2066.5 73 1670.0 74 1302.0 75 927.0 76 668.5 77 444.0 78 303.5 79 215.0 80 159.0 81 131.5 82 104.5 83 79.5 84 61.0 85 50.0 86 45.5 87 34.5 88 28.0 89 27.0 90 18.0 91 16.0 92 17.5 93 14.5 94 11.0 95 7.5 96 8.0 97 5.0 98 3.0 99 4.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012106370622103735 2 0.004394483286315235 3 0.007410305149472749 4 0.007453388318946427 5 0.0140020300789456 6 8.185802199998966E-4 7 0.00697947345473596 8 0.0 9 0.0024126574905260108 10-11 0.0 12-13 0.0033174040494732653 14-15 0.01757793314526094 16-17 0.028908806716838452 18-19 0.005773144709472955 20-21 0.008465842801577878 22-23 0.002132616888947099 24-25 0.011395498325788034 26-27 0.01777180740789249 28-29 5.169980336841452E-4 30-31 0.00140020300789456 32-33 0.00837967646263052 34-35 0.00840121804736736 36-37 0.010253794334735547 38-39 0.0 40-41 0.02408349173578643 42-43 0.00909054875894622 44-45 0.015876147951050627 46-47 0.0037051525747363746 48-49 0.023501868947891767 50-51 0.004717607057367825 52-53 0.0 54-55 0.016005397459471663 56-57 0.004329858532104716 58-59 2.8004060157891197E-4 60-61 0.004049817930525805 62-63 0.024665114523681093 64-65 0.016091563798419018 66-67 0.001960284211052384 68-69 0.01932280150894493 70-71 0.0027573228463154413 72-73 0.01090004187684073 74-75 0.0 76-77 0.005708519955262437 78-79 0.0010555376521051297 80-81 0.0033174040494732653 82-83 0.0014648277621050782 84-85 0.0 86-87 0.0051699803368414525 88-89 0.015833064781576946 90-91 0.020593755008418452 92-93 0.004329858532104716 94-95 0.02343724419368125 96-97 0.01604848062894534 98-99 7.539554657893785E-4 100-101 0.007991927937367412 102-103 0.0 104-105 0.0015294525163155963 106-107 0.0 108-109 0.0 110-111 6.031643726315027E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.1541584736839385E-5 122-123 0.0 124-125 0.023264911515786534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2321092.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.50910427096666 #Duplication Level Percentage of deduplicated Percentage of total 1 82.78045104119239 55.05653649896316 2 9.984567009014922 13.281292166060544 3 3.1384026006891315 6.261970374405192 4 1.3647877189310575 3.6308323482448177 5 0.7279819256256931 2.420871289940916 6 0.44735534294713875 1.7851921890147178 7 0.2963825140569943 1.3798494872055513 8 0.20586663366725902 1.0953604323590902 9 0.16310479917951667 0.9763158686152995 >10 0.8278344090635723 9.861546214189877 >50 0.04594226861935714 2.0797115944400413 >100 0.016929271184594117 1.8601110304578423 >500 1.9723291415110785E-4 0.06991779113416158 >1k 1.9723291414955666E-4 0.24049271496872382 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2588 0.11149924259788065 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.308316947367877E-5 2 0.0 0.0 0.0 0.0 4.308316947367877E-5 3 0.0 0.0 0.0 0.0 4.308316947367877E-5 4 0.0 0.0 0.0 0.0 8.616633894735754E-5 5 0.0 0.0 0.0 0.0 8.616633894735754E-5 6 4.308316947367877E-5 0.0 0.0 0.0 1.2924950842103632E-4 7 4.308316947367877E-5 0.0 0.0 0.0 1.2924950842103632E-4 8 4.308316947367877E-5 0.0 0.0 0.0 1.2924950842103632E-4 9 4.308316947367877E-5 0.0 0.0 0.0 1.7233267789471508E-4 10-11 4.308316947367877E-5 0.0 0.0 0.0 1.7233267789471508E-4 12-13 4.308316947367877E-5 0.0 0.0 0.0 2.1541584736839385E-4 14-15 4.308316947367877E-5 0.0 0.0 0.0 2.1541584736839385E-4 16-17 4.308316947367877E-5 0.0 0.0 0.0 2.1541584736839385E-4 18-19 6.462475421051816E-5 0.0 0.0 0.0 3.015821863157514E-4 20-21 8.616633894735754E-5 0.0 0.0 0.0 3.8774852526310893E-4 22-23 8.616633894735754E-5 0.0 0.0 1.2924950842103632E-4 4.308316947367877E-4 24-25 8.616633894735754E-5 0.0 0.0 1.9387426263155446E-4 5.169980336841453E-4 26-27 8.616633894735754E-5 0.0 0.0 3.4466535578943017E-4 5.60081203157824E-4 28-29 8.616633894735754E-5 0.0 0.0 4.954564489473059E-4 5.60081203157824E-4 30-31 8.616633894735754E-5 0.0 0.0 0.001507910931578757 5.60081203157824E-4 32-33 8.616633894735754E-5 0.0 0.0 0.004071359515262643 5.60081203157824E-4 34-35 8.616633894735754E-5 0.0 0.0 0.007216430886841194 5.60081203157824E-4 36-37 8.616633894735754E-5 0.0 0.0 0.013549656799471973 5.816227878946634E-4 38-39 1.2924950842103632E-4 0.0 0.0 0.02481590561683897 6.462475421051816E-4 40-41 1.2924950842103632E-4 0.0 0.0 0.03957189116157395 6.462475421051816E-4 42-43 1.2924950842103632E-4 0.0 0.0 0.054198627197887886 6.893307115788603E-4 44-45 1.2924950842103632E-4 0.0 0.0 0.07197043460578038 6.893307115788603E-4 46-47 1.507910931578757E-4 0.0 0.0 0.09338276983419873 6.893307115788603E-4 48-49 1.7233267789471508E-4 0.0 0.0 0.11944808736577438 6.893307115788603E-4 50-51 1.7233267789471508E-4 0.0 0.0 0.1480122287268234 7.324138810525391E-4 52-53 1.7233267789471508E-4 0.0 0.0 0.17668407801155664 8.832049742104148E-4 54-55 1.7233267789471508E-4 0.0 0.0 0.2074670026005001 9.047465589472542E-4 56-57 1.7233267789471508E-4 0.0 0.0 0.24460469468681123 9.047465589472542E-4 58-59 1.7233267789471508E-4 0.0 0.0 0.2873216572199637 9.047465589472542E-4 60-61 1.7233267789471508E-4 0.0 0.0 0.3367595941910101 9.047465589472542E-4 62-63 1.7233267789471508E-4 0.0 0.0 0.3866929876110038 9.478297284209329E-4 64-65 1.7233267789471508E-4 0.0 0.0 0.4411285722409969 9.478297284209329E-4 66-67 1.7233267789471508E-4 0.0 0.0 0.5032975857915154 9.478297284209329E-4 68-69 1.7233267789471508E-4 0.0 0.0 0.5778745521504534 9.478297284209329E-4 70-71 2.1541584736839385E-4 2.1541584736839385E-5 0.0 0.6605511543704429 9.478297284209329E-4 72-73 2.1541584736839385E-4 4.308316947367877E-5 0.0 0.7484408200967476 9.693713131577722E-4 74-75 2.1541584736839385E-4 4.308316947367877E-5 0.0 0.8376014393225257 0.0010124544826314512 76-77 2.1541584736839385E-4 4.308316947367877E-5 0.0 0.9355725667056713 0.0010770792368419693 78-79 2.1541584736839385E-4 4.308316947367877E-5 0.0 1.0502599638446042 0.0010986208215788087 80-81 2.1541584736839385E-4 8.616633894735754E-5 0.0 1.1825683772982716 0.001120162406315648 82-83 2.1541584736839385E-4 8.616633894735754E-5 0.0 1.3235149662314116 0.0012063287452630056 84-85 2.80040601578912E-4 8.616633894735754E-5 0.0 1.4684467483408672 0.001227870329999845 86-87 3.015821863157514E-4 8.616633894735754E-5 0.0 1.6208965435234794 0.0012709534994735238 88-89 3.015821863157514E-4 8.616633894735754E-5 0.0 1.7925183491218788 0.0012924950842103631 90-91 3.4466535578943017E-4 8.616633894735754E-5 0.0 1.9923811723102745 0.0012924950842103631 92-93 3.6620694052626955E-4 8.616633894735754E-5 0.0 2.2072800216449844 0.0013786614231577207 94-95 3.8774852526310893E-4 8.616633894735754E-5 0.0 2.4280166404433774 0.0013786614231577207 96-97 3.8774852526310893E-4 8.616633894735754E-5 0.0 2.652006038537033 0.0014217445926313994 98-99 4.308316947367877E-4 8.616633894735754E-5 0.0 2.8998635125191075 0.0014648277621050782 100-101 4.308316947367877E-4 8.616633894735754E-5 0.0 3.1860650073327554 0.0014648277621050782 102-103 4.308316947367877E-4 8.616633894735754E-5 0.0 3.4992150246521896 0.0014648277621050782 104-105 6.462475421051816E-4 8.616633894735754E-5 0.0 3.8173626896305706 0.0014648277621050782 106-107 6.462475421051816E-4 8.616633894735754E-5 0.0 4.129737209899478 0.0014863693468419176 108-109 6.67789126842021E-4 8.616633894735754E-5 0.0 4.4611760326604895 0.001572535685789275 110-111 6.893307115788603E-4 8.616633894735754E-5 0.0 4.846533442017809 0.0016371604399997932 112-113 7.108722963156997E-4 8.616633894735754E-5 0.0 5.258365459016704 0.0017017851942103114 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4465 0.0 54.23122 1 GGTATCA 2955 0.0 45.703014 1 ATCAACG 5410 0.0 43.549557 3 CAACGCA 5520 0.0 43.00507 5 TCAACGC 5535 0.0 42.888523 4 AACGCAG 5805 0.0 41.096035 6 TATCAAC 5960 0.0 39.43005 2 GTACATG 7920 0.0 35.23093 1 ACGCAGA 6945 0.0 33.750618 7 TACATGG 8120 0.0 33.41068 2 CGCAGAG 7025 0.0 32.352127 8 ACATGGG 8340 0.0 31.388638 3 GCAGAGT 8025 0.0 27.432238 9 CATGGGG 4765 0.0 25.845997 4 AGAGTAC 7145 0.0 23.73628 10-11 CAGAGTA 7660 0.0 22.295807 10-11 CATGGGA 4505 0.0 22.187088 4 GTGGTAT 1815 0.0 21.634346 1 GAGTACA 5560 0.0 21.614891 1 GAGTACT 4075 0.0 21.395718 12-13 >>END_MODULE