FastQCFastQC Report
Thu 26 May 2016
SRR1364522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364522_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321092
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40120.17284967592839923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34310.14781835446419184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37700.056.7668271
GTATCAA57800.051.1558531
ATCAACG75300.038.357293
TCAACGC77650.037.273144
CAACGCA77950.037.0189675
AACGCAG80250.036.0321246
TATCAAC81050.035.709552
GTACATG83750.030.1090491
ACGCAGA96200.030.0579837
TACATGG83550.029.2952022
CGCAGAG98000.029.202348
ACATGGG82700.028.5161463
TGGTATC19600.026.4340882
GTGGTAT20450.026.2355821
GCAGAGT107000.025.7996719
AGAGTAC99350.023.53448110-11
CAGAGTA104250.022.48537410-11
GAGTACT66700.022.16612812-13
GAGTACA63500.021.9676511
CATGGGA44700.021.716074
Produced by FastQC (version 0.11.5)