FastQCFastQC Report
Thu 26 May 2016
SRR1364521_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364521_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960944
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38100.055.9141921
GGTATCA21100.053.019961
ATCAACG43200.048.2124633
CAACGCA45000.046.5484475
TCAACGC44750.046.5425384
AACGCAG47200.044.6207246
TATCAAC48500.042.942792
ACGCAGA55350.037.405637
CGCAGAG55600.036.702428
GCAGAGT67700.029.2653089
GTACATG61450.027.695431
TACATGG59850.027.4415652
ACATGGG60350.025.8344383
AGAGTAC57200.024.7593610-11
CAGAGTA61900.023.98492210-11
GTGTAGC17000.022.4024241
GAGTACT35100.022.12588312-13
GTATAGG8350.022.092211
CATGGGG38100.021.3978754
GTATAAT12800.020.9202331
Produced by FastQC (version 0.11.5)