##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364521_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1960944 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.49642213138162 31.0 28.0 31.0 10.0 34.0 2 27.653329212868904 31.0 28.0 33.0 10.0 34.0 3 27.7511229285487 31.0 28.0 34.0 12.0 34.0 4 31.13694628709438 35.0 32.0 37.0 16.0 37.0 5 31.130870131936454 35.0 32.0 37.0 12.0 37.0 6 31.124042297995253 35.0 32.0 37.0 11.0 37.0 7 30.909416077154678 35.0 32.0 37.0 10.0 37.0 8 30.77725371045782 35.0 31.0 37.0 10.0 37.0 9 31.903768797069166 37.0 31.0 39.0 8.0 39.0 10-11 31.827072573209637 37.0 31.5 39.0 8.0 39.0 12-13 31.712661350859584 37.0 31.0 39.0 7.5 39.0 14-15 32.38999201405038 38.0 31.0 40.0 4.5 41.0 16-17 32.44772262746922 38.0 31.0 40.0 2.0 41.0 18-19 32.28946925562382 38.0 31.0 40.0 2.0 41.0 20-21 32.0444783226854 38.0 30.0 40.0 2.0 41.0 22-23 31.87654185943097 38.0 30.0 40.0 2.0 41.0 24-25 31.729314554622672 38.0 30.0 40.0 2.0 41.0 26-27 31.588376057653864 38.0 30.0 40.0 2.0 41.0 28-29 31.58687575983812 37.5 30.0 40.0 2.0 41.0 30-31 31.53421464355943 37.5 30.0 40.0 2.0 41.0 32-33 31.46877269315187 37.0 30.0 40.0 2.0 41.0 34-35 31.164095201086823 37.0 29.5 40.0 2.0 41.0 36-37 30.974022715590046 37.0 29.0 40.0 2.0 41.0 38-39 30.80135128795111 37.0 29.0 40.0 2.0 41.0 40-41 30.91554679786878 37.0 29.0 40.0 2.0 41.0 42-43 30.78152894728253 37.0 29.0 40.0 2.0 41.0 44-45 30.625547185437217 37.0 28.5 40.0 2.0 41.0 46-47 30.574602844344355 37.0 28.5 40.0 2.0 41.0 48-49 30.40056472800855 36.5 28.0 40.0 2.0 41.0 50-51 30.241106579280185 36.0 28.0 40.0 2.0 41.0 52-53 30.188668059873205 36.0 28.0 40.0 2.0 41.0 54-55 30.216592110738503 36.0 28.0 40.0 2.0 41.0 56-57 30.077702116939598 36.0 28.0 40.0 2.0 41.0 58-59 29.881886989123608 36.0 28.0 40.0 2.0 41.0 60-61 29.68775497923449 35.5 28.0 39.5 2.0 41.0 62-63 29.40509902373551 35.0 27.0 39.0 2.0 41.0 64-65 29.140867612741616 35.0 26.0 39.0 2.0 41.0 66-67 28.895666576913975 35.0 26.0 39.0 2.0 40.0 68-69 28.86166050636836 35.0 26.0 38.5 2.0 40.0 70-71 28.64205097136889 34.5 26.0 38.0 2.0 40.0 72-73 28.369668894165258 34.0 26.0 37.0 2.0 40.0 74-75 28.08115198598226 34.0 26.0 37.0 2.0 39.0 76-77 27.77935652420467 34.0 26.0 36.0 2.0 39.0 78-79 27.475900892631305 34.0 26.0 36.0 2.0 39.0 80-81 27.189922047748432 34.0 25.5 35.5 2.0 37.5 82-83 26.933591168335248 34.0 25.0 35.0 2.0 37.0 84-85 26.683320125408986 34.0 25.0 35.0 2.0 36.5 86-87 26.49266552231986 33.0 24.5 35.0 2.0 36.0 88-89 26.28931371829078 33.0 24.0 35.0 2.0 36.0 90-91 26.142657821946983 33.0 24.0 35.0 2.0 36.0 92-93 26.01186851842786 33.0 23.0 35.0 2.0 35.0 94-95 25.883833500599714 33.0 23.0 35.0 2.0 35.0 96-97 25.76211049372139 33.0 22.0 35.0 2.0 35.0 98-99 25.66643412560481 33.0 20.0 35.0 2.0 35.0 100-101 25.57623700625821 33.0 20.0 35.0 2.0 35.0 102-103 24.47758758026746 31.0 18.5 34.0 2.0 35.0 104-105 25.31708325173998 32.5 19.5 35.0 2.0 35.0 106-107 25.45783357403373 33.0 19.5 35.0 2.0 35.0 108-109 25.413440414412648 33.0 19.0 35.0 2.0 35.0 110-111 25.3259182822151 33.0 18.5 35.0 2.0 35.0 112-113 25.225448559469317 33.0 18.0 35.0 2.0 35.0 114-115 25.102042179684886 33.0 16.5 35.0 2.0 35.0 116-117 24.986948122944867 33.0 12.5 35.0 2.0 35.0 118-119 24.83789389192144 33.0 4.5 35.0 2.0 35.0 120-121 24.6539885891693 33.0 2.0 35.0 2.0 35.0 122-123 24.40975723937043 33.0 2.0 35.0 2.0 35.0 124-125 23.015621557780335 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23470186331495313 1101 2 0.2557484366729206 1101 3 0.21417264838492756 1101 4 0.25665717846245073 1101 5 0.22516657236185722 1101 6 0.32330507850060286 1101 7 0.4644648556415447 1101 8 0.19905005511155593 1101 9 0.4920165799867213 1101 10-11 0.5751801415033668 1101 12-13 0.312717930378156 1101 14-15 0.39032426749312066 1101 16-17 0.2553733782143581 1101 18-19 0.710100354551173 1101 20-21 1.0564696068678145 1101 22-23 0.9025916092301713 1101 24-25 1.0658150180972186 1101 26-27 1.241286166417023 1101 28-29 0.9759226570214921 1101 30-31 0.9776430210827876 1101 32-33 0.9539376518103779 1101 34-35 1.2844114891022471 1101 36-37 1.2987404112413294 1101 38-39 1.061955156901643 1101 40-41 0.7518798922129228 1101 42-43 0.8625967034477711 1101 44-45 0.7785023852096273 1101 46-47 0.7096417073173669 1101 48-49 0.6539909762692098 1101 50-51 0.5475307760707402 1101 52-53 0.3378973373416514 1101 54-55 0.24152429824874488 1101 56-57 0.20231618406008423 1101 58-59 0.22228593032499688 1101 60-61 0.17150547030140828 1101 62-63 0.11452059461516129 1101 64-65 0.2752083595369186 1101 66-67 0.14936077977718654 1101 68-69 -0.026359744756796033 1101 70-71 0.0074798886593967495 1101 72-73 -0.07406226623864498 1101 74-75 -0.0934060961091383 1101 76-77 0.08054521221155397 1101 78-79 0.1879228546460574 1101 80-81 0.29641549309829784 1101 82-83 0.11311122566476328 1101 84-85 0.2410588328803911 1101 86-87 0.21256431779567464 1101 88-89 0.1441484259168 1101 90-91 0.11215444881812431 1101 92-93 -0.05341821104608613 1101 94-95 0.03796875216484352 1101 96-97 -0.013961394123732873 1101 98-99 0.12151815817718514 1101 100-101 0.1623583903283361 1101 102-103 0.12974310486278284 1101 104-105 0.19323877068757866 1101 106-107 0.013113926422125388 1101 108-109 0.08454579628573455 1101 110-111 -0.055386136567580735 1101 112-113 0.061804338200413866 1101 114-115 0.19288803708846913 1101 116-117 0.3084359911414509 1101 118-119 0.19816622067869716 1101 120-121 0.23977569366649476 1101 122-123 0.33863692959372926 1101 124-125 0.5775171890768576 1102 1 -0.9054796382012391 1102 2 -0.5557649827066165 1102 3 -0.3615561358200985 1102 4 -0.37463933532488625 1102 5 -0.2798023911652123 1102 6 -1.263814947098595 1102 7 -1.7489175640971943 1102 8 -1.0331876066947103 1102 9 -0.7275723136167542 1102 10-11 -0.5720823575972673 1102 12-13 -0.5088070529589288 1102 14-15 -0.30831162141008406 1102 16-17 -0.5319218895362638 1102 18-19 -0.440503800904672 1102 20-21 -0.11070809534892234 1102 22-23 -0.24710259472024632 1102 24-25 -0.06038290385568601 1102 26-27 -0.5759572527463916 1102 28-29 0.0535543738355706 1102 30-31 -0.18471431368626057 1102 32-33 -0.35750614307907114 1102 34-35 -0.1344386371383024 1102 36-37 0.11252672597558089 1102 38-39 0.13580166330705268 1102 40-41 -0.1907144649296164 1102 42-43 -0.31564504609575295 1102 44-45 -0.18048637355852293 1102 46-47 -0.6189233287501246 1102 48-49 -0.9096760375624022 1102 50-51 -0.3554740564251375 1102 52-53 -0.6580835011961952 1102 54-55 -0.7933570697361532 1102 56-57 -0.7883982252182911 1102 58-59 -0.6799328339264754 1102 60-61 -0.7829318894977995 1102 62-63 -0.8951625441936422 1102 64-65 -0.7930819119384083 1102 66-67 -0.8349734819738721 1102 68-69 -0.9455868554663027 1102 70-71 -1.0044868604714843 1102 72-73 -0.6988121629418025 1102 74-75 -1.0602142732980369 1102 76-77 -0.8917717348817682 1102 78-79 -0.9738715111778795 1102 80-81 -1.3533998127811913 1102 82-83 -1.1251559990556395 1102 84-85 -1.425273617995586 1102 86-87 -0.8661305061637954 1102 88-89 -0.770974259547657 1102 90-91 -1.1590908921088818 1102 92-93 -0.783947040737285 1102 94-95 -0.8854266632334671 1102 96-97 -0.7676195523767824 1102 98-99 -0.5438923951446419 1102 100-101 -0.6256397792816628 1102 102-103 -0.7878185807484037 1102 104-105 -0.4916961858128488 1102 106-107 -0.6150760675492251 1102 108-109 -1.1323243225336803 1102 110-111 -0.8346106173225429 1102 112-113 -1.0251651307083556 1102 114-115 -1.0120615209487624 1102 116-117 -1.2036666328947838 1102 118-119 -0.8910344236054506 1102 120-121 -0.49974709725147903 1102 122-123 -0.5491734864306075 1102 124-125 -0.29670742344940493 1103 1 -0.21459686881805595 1103 2 -0.06541054672252145 1103 3 -0.16935006716415302 1103 4 -0.21343052472709445 1103 5 -0.17187322575883357 1103 6 0.04934368881273343 1103 7 0.08623277146739028 1103 8 0.11689644883673722 1103 9 0.3673060524401528 1103 10-11 0.2678066496488256 1103 12-13 0.11295223369783258 1103 14-15 0.34467273352125005 1103 16-17 0.2866521363182102 1103 18-19 0.3421030603061652 1103 20-21 0.6402897216182382 1103 22-23 0.7418890895904084 1103 24-25 0.7573278267589387 1103 26-27 0.6388521647608307 1103 28-29 0.6118559808633606 1103 30-31 0.5774218201099792 1103 32-33 0.4044242354886336 1103 34-35 0.8571941662177807 1103 36-37 0.7613372086976185 1103 38-39 0.8575300455832817 1103 40-41 0.5905678641209384 1103 42-43 0.3431337431977468 1103 44-45 0.07409928308285174 1103 46-47 0.10021037324397497 1103 48-49 0.01757620136133653 1103 50-51 0.001680170680412374 1103 52-53 0.01509007183238964 1103 54-55 -0.1671910321160972 1103 56-57 -0.17718546938043644 1103 58-59 -0.34790060789052646 1103 60-61 -0.468445433726135 1103 62-63 -0.37173316070315465 1103 64-65 -0.09719786583170276 1103 66-67 -0.07569545542678924 1103 68-69 -0.04221564782831777 1103 70-71 -0.07643625838401036 1103 72-73 -0.12857606772124086 1103 74-75 -0.1460165323863407 1103 76-77 -0.11851988719433848 1103 78-79 -0.14802084618574796 1103 80-81 -0.11784035034207108 1103 82-83 7.392142722544293E-4 1103 84-85 -0.12502043769373117 1103 86-87 -0.1924228188635233 1103 88-89 -0.22601948186193255 1103 90-91 -0.141773890973532 1103 92-93 -0.06703828975645365 1103 94-95 -0.11830596518899128 1103 96-97 0.027136730418025223 1103 98-99 -0.13429510552456847 1103 100-101 -0.20268223554429454 1103 102-103 -0.29929977729587876 1103 104-105 -0.2286156601753646 1103 106-107 -0.19268400822158327 1103 108-109 -0.08600734112452812 1103 110-111 -0.048623726478567164 1103 112-113 0.01784209565636985 1103 114-115 0.03465738179230016 1103 116-117 0.013028965532377867 1103 118-119 -0.0046000767837810486 1103 120-121 0.07158256920039108 1103 122-123 0.13487346959749758 1103 124-125 0.3306797207666534 1104 1 0.3889881602527083 1104 2 0.5078135300806679 1104 3 0.3637393905966917 1104 4 0.30912351856501274 1104 5 0.2800199314942162 1104 6 0.349413355330249 1104 7 0.42565803381579315 1104 8 0.4948606539645226 1104 9 0.8055836217714223 1104 10-11 0.6840125218195752 1104 12-13 0.7403732435322183 1104 14-15 0.5957715248507824 1104 16-17 0.6287476634301612 1104 18-19 0.8712735412702557 1104 20-21 1.1380574168794837 1104 22-23 0.841013956346572 1104 24-25 1.2541763276545268 1104 26-27 1.2718726658397763 1104 28-29 1.0747455181257948 1104 30-31 1.0115497160682558 1104 32-33 1.0833081566684797 1104 34-35 1.2937218624642384 1104 36-37 1.3329264827564238 1104 38-39 1.3394983821254094 1104 40-41 1.165550836618312 1104 42-43 1.1508762623855446 1104 44-45 1.07292989304203 1104 46-47 1.0168090496314584 1104 48-49 0.8589882337421564 1104 50-51 0.6736549111371417 1104 52-53 0.8421694967937015 1104 54-55 0.6405760747512801 1104 56-57 0.7460439269137815 1104 58-59 0.7112409288847772 1104 60-61 0.7140964499962941 1104 62-63 0.6845439327816827 1104 64-65 0.6348928532129676 1104 66-67 0.6817623940210105 1104 68-69 0.7400153364451469 1104 70-71 0.695136672351083 1104 72-73 0.6659024459821801 1104 74-75 0.6933228227476746 1104 76-77 0.7576043470384164 1104 78-79 0.8209820434438768 1104 80-81 0.7652939810030404 1104 82-83 0.7738718591163511 1104 84-85 0.735915537870131 1104 86-87 0.7649706664533156 1104 88-89 0.7839374165771815 1104 90-91 0.8705910606618623 1104 92-93 0.8759615679343291 1104 94-95 0.8292258936019543 1104 96-97 0.5355505060867074 1104 98-99 0.6569077733634536 1104 100-101 0.7458064902135391 1104 102-103 0.8178927150721051 1104 104-105 0.8313465064413563 1104 106-107 0.8956536234657513 1104 108-109 0.9662369452973927 1104 110-111 0.9326445667550409 1104 112-113 0.9718783578227814 1104 114-115 0.9606526140915648 1104 116-117 0.9668999129696516 1104 118-119 0.9315673137441713 1104 120-121 0.9973471904457867 1104 122-123 0.9892732274665832 1104 124-125 1.2824718761081044 1105 1 -0.5932852936776065 1105 2 -0.24550563917176405 1105 3 -0.30846918760619957 1105 4 -0.18786459564050872 1105 5 -0.21029672175416536 1105 6 -0.27389380735881375 1105 7 -0.1431512778388715 1105 8 -0.4115458013279394 1105 9 -0.4456512639934509 1105 10-11 -0.29402546906884197 1105 12-13 -0.22951523495081716 1105 14-15 -0.20875252461080152 1105 16-17 -0.3493934715560272 1105 18-19 -0.10938416210485968 1105 20-21 0.273413381660113 1105 22-23 0.37975845652181306 1105 24-25 0.3686649738595946 1105 26-27 0.2356963929239697 1105 28-29 -0.00965357243498488 1105 30-31 -0.19825306251912522 1105 32-33 -0.07591260009142431 1105 34-35 0.6239365628076285 1105 36-37 0.5783158417410412 1105 38-39 0.5423767449333887 1105 40-41 0.23054668176915882 1105 42-43 -0.04863159788060045 1105 44-45 0.10072918087892191 1105 46-47 -0.02242568888393137 1105 48-49 -0.06757512212920247 1105 50-51 0.02131711282778781 1105 52-53 0.06702328265526702 1105 54-55 -0.12663199424274296 1105 56-57 -0.10507920204750931 1105 58-59 -0.23898666099796984 1105 60-61 -0.23066016162285408 1105 62-63 -0.13618281289093304 1105 64-65 -0.11302418732935138 1105 66-67 -0.3251124031962185 1105 68-69 -0.4874897649768428 1105 70-71 -0.2551491748461423 1105 72-73 -0.17259369223507903 1105 74-75 -0.15960189847024253 1105 76-77 -0.09286414682420485 1105 78-79 -0.026297188031882968 1105 80-81 0.017422391584617003 1105 82-83 -0.13457859442682363 1105 84-85 -0.17288198514345865 1105 86-87 -0.1488884708331284 1105 88-89 0.013865145383213928 1105 90-91 -0.05568682568636518 1105 92-93 -0.15859335443106914 1105 94-95 -0.10440344071836449 1105 96-97 -0.034515765732543 1105 98-99 -0.16254199975860217 1105 100-101 -0.15097335626324337 1105 102-103 -0.17150658082183412 1105 104-105 -0.1466226783043929 1105 106-107 -0.148615250276233 1105 108-109 -0.10822901267902907 1105 110-111 -0.06363204418342505 1105 112-113 -0.03403159060777483 1105 114-115 -0.18182176940322492 1105 116-117 -0.098137736796577 1105 118-119 -0.059715360382902816 1105 120-121 -0.224258945481985 1105 122-123 -0.0379998778748849 1105 124-125 0.01130109422635428 1106 1 -0.21880197346810348 1106 2 0.0675560785906697 1106 3 -0.1351717421086711 1106 4 -0.0011162934080601872 1106 5 -0.13696298712495647 1106 6 -0.3570469434089212 1106 7 -0.5777766840261123 1106 8 -0.1973103657580353 1106 9 -0.25548595460567824 1106 10-11 -0.003595204532238938 1106 12-13 -0.06438034733137243 1106 14-15 0.14069814945277415 1106 16-17 0.06198337550584654 1106 18-19 0.06881472212917572 1106 20-21 0.38127799096816517 1106 22-23 0.28083039770527307 1106 24-25 0.6693009097964264 1106 26-27 0.7970892600440997 1106 28-29 0.666225694522744 1106 30-31 0.4493645337421732 1106 32-33 0.2839036735821949 1106 34-35 0.6563239545995998 1106 36-37 0.5939824055415919 1106 38-39 0.7365444359486375 1106 40-41 0.4032859446757904 1106 42-43 0.4173647957223814 1106 44-45 0.21541418585925598 1106 46-47 0.0825864058287955 1106 48-49 0.12390838066084697 1106 50-51 0.13011718640474967 1106 52-53 0.0021286550537524818 1106 54-55 -0.12678070977363376 1106 56-57 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0.11615704848557229 2216 68-69 0.2625574351137807 2216 70-71 0.29377109685855274 2216 72-73 0.2535150903382295 2216 74-75 0.3928625755948829 2216 76-77 0.4500931800983068 2216 78-79 0.14829175162332575 2216 80-81 0.24107005654369118 2216 82-83 0.41980220331764073 2216 84-85 0.4085769801466448 2216 86-87 0.3445979166607316 2216 88-89 0.1842406902820386 2216 90-91 0.32453088210941416 2216 92-93 0.16085685891334123 2216 94-95 0.1518427046075672 2216 96-97 0.2975788274446316 2216 98-99 0.2595820462751206 2216 100-101 0.2735012542835662 2216 102-103 0.43133402552101785 2216 104-105 0.23798254013762588 2216 106-107 0.27450104655149943 2216 108-109 0.22683499064386226 2216 110-111 0.24881367571235558 2216 112-113 0.3125408437001873 2216 114-115 0.25057091856851343 2216 116-117 0.22038420809889203 2216 118-119 0.2816870906653577 2216 120-121 0.32207161458380185 2216 122-123 0.40526666208576145 2216 124-125 0.4058949007353547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 163435.0 3 25453.0 4 15602.0 5 11877.0 6 11168.0 7 11923.0 8 12446.0 9 13155.0 10 13609.0 11 14288.0 12 15055.0 13 16106.0 14 16692.0 15 14974.0 16 12545.0 17 11375.0 18 10434.0 19 9712.0 20 9870.0 21 10474.0 22 12451.0 23 16155.0 24 21126.0 25 27153.0 26 33056.0 27 37523.0 28 43325.0 29 50587.0 30 57575.0 31 66384.0 32 80402.0 33 100542.0 34 128638.0 35 174958.0 36 261505.0 37 324257.0 38 104740.0 39 374.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.451666619914775 18.814661053039593 13.68568958614376 24.04798274090187 2 14.944704679384039 19.55447551680789 41.35350501189544 24.14731479191263 3 19.49656666544266 24.441241630864514 28.435527566636882 27.62666413705594 4 12.06995826637406 16.471853645176967 38.25524577346949 33.20294231497948 5 13.493962753396557 37.790756387383446 34.65870200983137 14.056578849388627 6 31.977647295206445 36.86768394115841 17.673945880670665 13.480722882964477 7 28.070665755464663 32.20626485378268 21.07217535878579 18.65089403196687 8 25.16298272668674 36.385791741120606 19.63982653252719 18.811398999665467 9 25.471901139171948 16.93912686942411 19.79230944189856 37.79666254950538 10-11 24.67059232696089 26.948092347359232 27.663232606336543 20.718082719343336 12-13 25.411648370758538 23.944873350633507 27.822266329706792 22.821211948901162 14-15 22.242706643506718 25.636288658111155 25.595586178192704 26.525418520189426 16-17 22.267228265915236 28.778016623528202 26.157444012663017 22.797311097893544 18-19 22.114518123748933 27.71583389644665 28.070225542819987 22.099422436984433 20-21 22.826379377656647 26.885149251580952 28.26318177100639 22.025289599756015 22-23 22.375061673935797 26.821169172083987 28.045519508087413 22.758249645892803 24-25 22.142282461095135 26.89068387129679 28.355993038245025 22.61104062936305 26-27 22.145082075495274 27.184170138015773 27.974959361370377 22.695788425118575 28-29 22.101477637320368 27.34777729699794 28.01381178208655 22.536933283595143 30-31 22.347067854448227 27.131860429122085 27.836711568427013 22.684360148002675 32-33 22.12942104680678 27.04707300168353 27.86930393933382 22.954202012175873 34-35 22.46620320762887 27.131198416126782 28.001599377385233 22.400998998859116 36-37 22.517121191720626 26.813790592065807 28.003833270091516 22.66525494612205 38-39 22.209328772264787 27.17125017338588 27.922826964971975 22.69659408937736 40-41 22.71257766034502 27.025927588423126 27.695591850892136 22.565902900339715 42-43 22.4430520708674 27.00748000584467 27.64447774218218 22.90499018110575 44-45 22.49506661409829 27.218493528325975 27.641089226321657 22.645350631254075 46-47 22.735971379868246 27.397069904365818 27.06257338944824 22.804385326317693 48-49 22.654778330651144 27.336499248902683 27.2114013767142 22.797321043731973 50-51 22.72003329166775 27.551455974982293 27.018320106933164 22.710190626416797 52-53 22.724310332166546 27.44583731100939 27.04735576334663 22.78249659347743 54-55 22.659606942855152 27.35336098975546 27.138172365628883 22.848859701760503 56-57 22.58756613534002 27.575411945810142 27.03465059605635 22.802371322793498 58-59 22.634269925923206 27.321389049526296 27.143285509281785 22.901055515268716 60-61 22.57355723577584 27.397730784647912 27.218956638281078 22.809755341295173 62-63 22.56446865342518 27.359157776395428 27.32875627509176 22.747617295087633 64-65 22.51403966784751 27.3703455386614 27.140977621454187 22.974637172036903 66-67 22.502843711744397 27.467845849787253 27.081927201489314 22.94738323697904 68-69 22.727828000580452 27.193252476420415 27.29363290703104 22.78528661596809 70-71 22.604053781838303 27.139072408631815 27.184205091068574 23.072668718461305 72-73 22.58128762194259 27.24037114153947 27.199136026153823 22.979205210364114 74-75 22.545110926166174 27.489260274643236 27.038380494292547 22.927248304898047 76-77 22.666853663978582 27.284633283396552 27.156472125713194 22.892040926911676 78-79 22.708815514730688 27.305654708100548 27.118982459030143 22.866547318138622 80-81 22.68543722246092 27.3131220923339 27.014326530664285 22.9871141545409 82-83 22.685958399905353 27.483886299670203 27.102022582383988 22.72813271804046 84-85 22.67729216132638 27.541913486565655 26.992841203012425 22.78795314909554 86-87 22.670018316265267 27.39626754140029 27.038280560916817 22.895433581417624 88-89 22.780975755554127 27.334600283380617 26.967470394478465 22.91695356658679 90-91 22.634612917647594 27.583020724864 27.00146568294781 22.7809006745406 92-93 22.69185311925869 27.40893263992391 27.11028550227772 22.788928738539685 94-95 22.50625107755213 27.393925513475903 27.120189016670558 22.979634392301406 96-97 22.72673254095806 27.23108655857407 27.177914141720684 22.86426675874718 98-99 22.47227138409899 27.321104243888918 27.039247538980955 23.167376833031135 100-101 22.520511001506037 27.27520773652486 27.204368472294398 22.999912789674706 102-103 22.300585840289166 27.107199389681703 27.421359304498242 23.170855465530888 104-105 22.26839952675275 27.2153886951023 27.22025885808702 23.295952920057932 106-107 22.297307978865273 27.24974667800135 27.15374694725956 23.299198395873823 108-109 22.324859863412723 27.202765606768985 27.114670281252295 23.357704248565998 110-111 22.280457017493983 27.241721600168084 27.18320349282981 23.294617889508125 112-113 22.437382199593664 27.263654647965467 27.168853368581665 23.1301097838592 114-115 22.273813020667596 27.36773717148475 27.098147626857266 23.26030218099038 116-117 22.2103231912793 27.326940493966173 27.263501660424776 23.19923465432975 118-119 22.137169648903793 27.388721962483377 27.081701466232587 23.39240692238024 120-121 22.0823491134882 27.43719351496014 27.19310189378177 23.287355477769893 122-123 22.107796041090413 27.423450134221067 27.142845486663564 23.325908338024952 124-125 22.144081103544377 27.401009560810696 26.96845358369095 23.486455751953976 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 271.0 1 278.5 2 334.0 3 355.0 4 300.5 5 264.5 6 264.0 7 332.5 8 458.0 9 539.0 10 567.5 11 582.5 12 635.5 13 703.5 14 801.5 15 883.5 16 970.0 17 1145.0 18 1373.5 19 1681.5 20 2033.0 21 2513.5 22 3243.0 23 4322.5 24 5893.5 25 7920.5 26 10717.5 27 14158.0 28 17281.5 29 20564.5 30 23993.0 31 27924.0 32 32705.5 33 36338.5 34 39393.5 35 43104.0 36 47377.5 37 51042.5 38 53584.5 39 56228.5 40 59595.0 41 62989.5 42 64859.5 43 66196.5 44 68597.5 45 71089.0 46 72278.0 47 72591.0 48 72428.5 49 72122.0 50 70965.0 51 68960.5 52 67740.5 53 66952.0 54 65585.5 55 61577.5 56 56456.0 57 51692.5 58 47113.0 59 41995.5 60 35209.5 61 29070.5 62 23822.5 63 18401.0 64 13539.5 65 10185.5 66 7839.5 67 6149.0 68 4973.5 69 3938.0 70 3064.5 71 2357.5 72 1826.5 73 1464.5 74 1131.5 75 851.0 76 643.0 77 465.0 78 355.5 79 261.5 80 174.0 81 121.0 82 81.5 83 55.5 84 44.5 85 32.5 86 23.5 87 22.0 88 20.5 89 15.0 90 14.0 91 11.5 92 8.5 93 8.0 94 6.5 95 4.0 96 4.0 97 4.0 98 1.5 99 4.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012188007408676638 2 0.005558547311906918 3 0.008567302278902407 4 0.008924273207190006 5 0.014380828825300469 6 9.17925244168115E-4 7 0.008363318891309491 8 0.0 9 0.002345808957318516 10-11 0.0 12-13 0.0039521781346127174 14-15 0.019148940510284842 16-17 0.030087549669954878 18-19 0.006068505780889204 20-21 0.009051762824435578 22-23 0.0013513899428030582 24-25 0.011805538556939922 26-27 0.018919459199242816 28-29 4.844605455331718E-4 30-31 0.0017083608710906583 32-33 0.009230248288579377 34-35 0.009230248288579377 36-37 0.011015102930017378 38-39 0.0 40-41 0.024681989898742644 42-43 0.00935773790582495 44-45 0.01647165854812784 46-47 0.004411140756696775 48-49 0.0244270106642515 50-51 0.004742613761535261 52-53 0.0 54-55 0.017262094175050386 56-57 0.004972095072577289 58-59 3.0597508138937164E-4 60-61 0.004589626220840574 62-63 0.026237363229138617 64-65 0.017262094175050386 66-67 0.0019633401055818016 68-69 0.020525828376537016 70-71 0.003008754966995488 72-73 0.012060517791431066 74-75 0.0 76-77 0.006705953867117063 78-79 0.0010454148614136864 80-81 0.0036462030532233456 82-83 0.0017848546414380012 84-85 0.0 86-87 0.00604300785744009 88-89 0.01682862947641544 90-91 0.021647737008298047 92-93 0.005176078460170204 94-95 0.024579998204946187 96-97 0.017389583792295955 98-99 9.434231676172292E-4 100-101 0.008516306432004177 102-103 0.0 104-105 0.0012239003255574866 106-107 5.099584689822861E-5 108-109 0.0 110-111 6.374480862278576E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.019811886519961814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1960944.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.69743300740883 #Duplication Level Percentage of deduplicated Percentage of total 1 84.22925328219928 59.547919911823534 2 9.680267885183559 13.687401806130714 3 2.849027273388442 6.0425674438998005 4 1.1767465541701392 3.3277184272057037 5 0.591299356351502 2.0901673316492135 6 0.3474564390854232 1.4738566995140718 7 0.21238768180813988 1.0510684734360884 8 0.15770286915780612 0.8919350421888126 9 0.11528796929087054 0.7335507136951371 >10 0.5975586816983032 7.549186781790847 >50 0.029766357438334622 1.440165546658411 >100 0.012300217278950107 1.5782263905154155 >500 7.999817263085285E-4 0.4150230137699885 >1k 1.4545122296518142E-4 0.17121241772225282 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.099584689822861E-5 7 0.0 0.0 0.0 0.0 5.099584689822861E-5 8 0.0 0.0 0.0 0.0 5.099584689822861E-5 9 0.0 0.0 0.0 0.0 5.099584689822861E-5 10-11 0.0 0.0 0.0 0.0 5.099584689822861E-5 12-13 0.0 0.0 0.0 0.0 2.2948131104202874E-4 14-15 0.0 0.0 0.0 0.0 2.5497923449114304E-4 16-17 0.0 0.0 0.0 0.0 3.0597508138937164E-4 18-19 0.0 5.099584689822861E-5 0.0 7.649377034734292E-5 3.5697092828760024E-4 20-21 0.0 5.099584689822861E-5 0.0 1.7848546414380015E-4 4.079667751858289E-4 22-23 0.0 5.099584689822861E-5 0.0 3.3147300483848594E-4 4.334646986349432E-4 24-25 0.0 5.099584689822861E-5 0.0 4.844605455331718E-4 5.099584689822861E-4 26-27 0.0 5.099584689822861E-5 0.0 7.139418565752005E-4 5.609543158805147E-4 28-29 0.0 5.099584689822861E-5 0.0 9.17925244168115E-4 5.609543158805147E-4 30-31 0.0 5.099584689822861E-5 0.0 0.0017848546414380012 5.864522393296289E-4 32-33 0.0 5.099584689822861E-5 0.0 0.00367170097667246 6.629460096769719E-4 34-35 0.0 1.0199169379645722E-4 0.0 0.006705953867117062 7.649377034734291E-4 36-37 0.0 1.0199169379645722E-4 0.0 0.012366492872820437 7.649377034734291E-4 38-39 0.0 1.0199169379645722E-4 0.0 0.021188774386213985 7.904356269225434E-4 40-41 0.0 1.0199169379645722E-4 0.0 0.033682756876279994 8.159335503716578E-4 42-43 0.0 1.0199169379645722E-4 0.0 0.046100245595998665 8.41431473820772E-4 44-45 0.0 1.0199169379645722E-4 0.0 0.05986912425852039 8.669293972698864E-4 46-47 0.0 1.0199169379645722E-4 0.0 0.0766467578880376 9.17925244168115E-4 48-49 0.0 1.0199169379645722E-4 0.0 0.0969941008004308 9.434231676172292E-4 50-51 0.0 1.0199169379645722E-4 0.0 0.12019721113912482 0.0010199169379645722 52-53 0.0 1.0199169379645722E-4 0.0 0.1440122716405976 0.0011219086317610294 54-55 0.0 1.0199169379645722E-4 0.0 0.16907673039107696 0.001249398249006601 56-57 0.0 1.0199169379645722E-4 0.0 0.2014080973245539 0.0012748961724557153 58-59 0.0 1.0199169379645722E-4 0.0 0.2359832815215529 0.0012748961724557153 60-61 0.0 1.0199169379645722E-4 0.0 0.2773409133560163 0.0013003940959048295 62-63 0.0 1.0199169379645722E-4 0.0 0.3199224455160372 0.001427883713150401 64-65 0.0 1.0199169379645722E-4 0.0 0.3670936038968986 0.001427883713150401 66-67 0.0 1.0199169379645722E-4 0.0 0.422730072862866 0.0014533816365995154 68-69 0.0 1.0199169379645722E-4 0.0 0.48637288979185533 0.0014788795600486297 70-71 0.0 1.0199169379645722E-4 0.0 0.5585575110762979 0.0016318671007433156 72-73 0.0 1.0199169379645722E-4 0.0 0.6325014890787295 0.0018613484117853442 74-75 0.0 1.0199169379645722E-4 0.0 0.7084343051101918 0.0019633401055818016 76-77 0.0 1.0199169379645722E-4 0.0 0.7947447759854438 0.001988838029030916 78-79 2.5497923449114306E-5 1.0199169379645722E-4 0.0 0.8952575902218524 0.001988838029030916 80-81 5.099584689822861E-5 1.0199169379645722E-4 0.0 1.0110946564511787 0.00201433595248003 82-83 5.099584689822861E-5 1.0199169379645722E-4 0.0 1.1348615768731793 0.002090829722827373 84-85 5.099584689822861E-5 1.0199169379645722E-4 0.0 1.262580675429793 0.002090829722827373 86-87 5.099584689822861E-5 1.0199169379645722E-4 0.0 1.3988160804184107 0.0021928214166238302 88-89 5.099584689822861E-5 1.0199169379645722E-4 0.0 1.5513191605675634 0.0023713068807676304 90-91 5.099584689822861E-5 1.0199169379645722E-4 5.099584689822861E-5 1.7302635873334475 0.0023968048042167446 92-93 5.099584689822861E-5 1.0199169379645722E-4 5.099584689822861E-5 1.9265466020447295 0.0025497923449114305 94-95 5.099584689822861E-5 1.0199169379645722E-4 5.099584689822861E-5 2.1291276038479428 0.0025497923449114305 96-97 5.099584689822861E-5 1.0199169379645722E-4 5.099584689822861E-5 2.336425721489242 0.0026517840387078875 98-99 5.099584689822861E-5 1.0199169379645722E-4 5.099584689822861E-5 2.561266410463532 0.0026772819621570017 100-101 5.099584689822861E-5 1.0199169379645722E-4 5.099584689822861E-5 2.814970748782219 0.0027027798856061164 102-103 5.099584689822861E-5 1.0199169379645722E-4 5.099584689822861E-5 3.094224006396919 0.0027282778090552307 104-105 5.099584689822861E-5 1.0199169379645722E-4 5.099584689822861E-5 3.388240561688656 0.002779273655953459 106-107 5.099584689822861E-5 1.0199169379645722E-4 5.099584689822861E-5 3.6781519513050855 0.0028047715794025734 108-109 5.099584689822861E-5 1.0199169379645722E-4 5.099584689822861E-5 3.981959709201283 0.002855767426300802 110-111 7.649377034734292E-5 1.0199169379645722E-4 5.099584689822861E-5 4.3201641658303345 0.0028812653497499166 112-113 1.0199169379645722E-4 1.0199169379645722E-4 5.099584689822861E-5 4.702072063251169 0.002906763273199031 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3810 0.0 55.914192 1 GGTATCA 2110 0.0 53.01996 1 ATCAACG 4320 0.0 48.212463 3 CAACGCA 4500 0.0 46.548447 5 TCAACGC 4475 0.0 46.542538 4 AACGCAG 4720 0.0 44.620724 6 TATCAAC 4850 0.0 42.94279 2 ACGCAGA 5535 0.0 37.40563 7 CGCAGAG 5560 0.0 36.70242 8 GCAGAGT 6770 0.0 29.265308 9 GTACATG 6145 0.0 27.69543 1 TACATGG 5985 0.0 27.441565 2 ACATGGG 6035 0.0 25.834438 3 AGAGTAC 5720 0.0 24.75936 10-11 CAGAGTA 6190 0.0 23.984922 10-11 GTGTAGC 1700 0.0 22.402424 1 GAGTACT 3510 0.0 22.125883 12-13 GTATAGG 835 0.0 22.09221 1 CATGGGG 3810 0.0 21.397875 4 GTATAAT 1280 0.0 20.920233 1 >>END_MODULE