FastQCFastQC Report
Thu 26 May 2016
SRR1364521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960944
Sequences flagged as poor quality0
Sequence length125
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30480.15543534134580078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29050.1481429352393541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27050.059.4652021
GTATCAA44650.052.4369541
ATCAACG59300.038.659013
TCAACGC60600.037.829694
CAACGCA61150.037.363465
AACGCAG63550.035.952416
TATCAAC64100.035.9498982
ACGCAGA77450.029.2695377
CGCAGAG78000.028.9868688
GTACATG57600.024.8230511
AGAGTAC78900.024.46996910-11
ACATGGG56900.023.7551773
TACATGG58150.023.5517312
GAGTACT58050.023.47175612-13
GCAGAGT92700.023.4274759
CAGAGTA83650.022.36919810-11
TGGTATC12950.022.0707042
GTACTTT62850.021.96317114-15
AGTACTT64500.021.63193912-13
TAGCCCT18950.020.1101574
Produced by FastQC (version 0.11.5)