FastQCFastQC Report
Thu 26 May 2016
SRR1364520_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364520_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914122
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.22589991651524827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29680.15505803705301963No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27940.1459677073875124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84600.051.624681
GGTATCA45900.049.649831
ATCAACG103900.041.4051553
TCAACGC104050.041.288284
CAACGCA104600.041.2418335
AACGCAG107000.040.0891086
TATCAAC113050.038.632882
GTACATG134050.038.1292271
TACATGG140500.035.870612
ACATGGG142750.034.805033
ACGCAGA123400.034.5201577
CGCAGAG122500.033.8527768
CATGGGA94000.030.7005044
GCAGAGT129600.030.6209359
ATGGGAT36450.030.5264115
CATGGGG44150.030.0542454
GTATAGG7100.029.3319781
GAGTACT47900.027.9534512-13
ATGGGTA11100.025.1945485
CATGGGT17650.024.6098984
Produced by FastQC (version 0.11.5)