##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364520_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1914122 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.720071656874534 31.0 29.0 33.0 22.0 34.0 2 28.757163336506242 31.0 28.0 34.0 20.0 34.0 3 28.96006889842967 31.0 29.0 34.0 22.0 34.0 4 32.30808328831704 35.0 33.0 37.0 22.0 37.0 5 32.491089387196844 35.0 33.0 37.0 25.0 37.0 6 32.43676839825257 35.0 33.0 37.0 23.0 37.0 7 32.255088233665354 35.0 32.0 37.0 22.0 37.0 8 32.08719350177262 35.0 32.0 37.0 19.0 37.0 9 33.2127711817742 38.0 33.0 39.0 17.0 39.0 10-11 33.18812959675506 37.5 33.0 39.0 17.0 39.0 12-13 33.10045571807858 38.0 33.0 39.0 17.0 39.0 14-15 33.84549495800164 38.0 32.5 41.0 16.0 41.0 16-17 33.96826142743252 38.5 33.0 41.0 17.0 41.0 18-19 33.83610527437645 38.5 32.5 41.0 16.5 41.0 20-21 33.61243954147123 39.0 32.0 41.0 14.0 41.0 22-23 33.48644208676354 38.5 32.0 41.0 14.0 41.0 24-25 33.35852599782041 38.0 32.0 41.0 12.5 41.0 26-27 33.28149538012728 38.0 32.0 40.0 13.0 41.0 28-29 33.32075411076201 38.0 32.0 40.0 12.5 41.0 30-31 33.260555231066775 38.0 32.0 40.0 12.5 41.0 32-33 33.21208444393827 38.0 32.0 40.0 10.0 41.0 34-35 32.827376468166605 38.0 31.0 40.0 9.0 41.0 36-37 32.63260753494291 38.0 30.5 40.0 8.5 41.0 38-39 32.44796047482867 38.0 30.0 40.0 8.0 41.0 40-41 32.60207290862338 38.0 31.0 40.0 8.0 41.0 42-43 32.463859147954 38.0 31.0 40.0 4.5 41.0 44-45 32.29606942504188 38.0 30.5 40.0 2.0 41.0 46-47 32.1887327453527 38.0 31.0 40.0 2.0 41.0 48-49 31.977376833869524 37.5 30.0 40.0 2.0 41.0 50-51 31.816479043655526 37.0 30.0 40.0 2.0 41.0 52-53 31.754352648368286 37.0 30.0 40.0 2.0 41.0 54-55 31.770832005483456 37.0 30.0 40.0 2.0 41.0 56-57 31.64969552619948 37.0 30.0 40.0 2.0 41.0 58-59 31.464657425179794 37.0 30.0 40.0 2.0 41.0 60-61 31.272098643660122 36.0 30.0 40.0 2.0 41.0 62-63 31.004052249543133 36.0 30.0 40.0 2.0 41.0 64-65 30.73918799324181 35.5 29.0 39.5 2.0 41.0 66-67 30.501942404925078 35.0 29.0 39.0 2.0 41.0 68-69 30.45907444771023 35.0 29.0 39.0 2.0 41.0 70-71 30.2265735935327 35.0 29.0 39.0 2.0 40.5 72-73 29.946240365034207 35.0 29.0 38.0 2.0 40.0 74-75 29.63893524028249 35.0 29.0 37.0 2.0 39.5 76-77 29.32946254209502 35.0 29.0 37.0 2.0 39.0 78-79 29.003523025178126 34.5 29.0 36.0 2.0 39.0 80-81 28.686213313466958 34.0 29.0 36.0 2.0 38.0 82-83 28.417402861468602 34.0 28.5 35.5 2.0 37.0 84-85 28.144501761120765 34.0 28.0 35.0 2.0 37.0 86-87 27.937832332526348 34.0 28.0 35.0 2.0 36.0 88-89 27.72492035512888 34.0 27.5 35.0 2.0 36.0 90-91 27.57522456771303 34.0 27.0 35.0 2.0 36.0 92-93 27.44449256630455 34.0 27.0 35.0 2.0 35.5 94-95 27.310447818895554 34.0 27.0 35.0 2.0 35.0 96-97 27.190877070531556 34.0 26.5 35.0 2.0 35.0 98-99 27.09568381743692 34.0 26.0 35.0 2.0 35.0 100-101 27.009464913939656 34.0 26.0 35.0 2.0 35.0 102-103 25.863961388041098 32.0 24.5 34.0 2.0 35.0 104-105 26.721131934119143 33.5 25.0 35.0 2.0 35.0 106-107 26.860291298046832 34.0 25.5 35.0 2.0 35.0 108-109 26.807840357093227 34.0 25.0 35.0 2.0 35.0 110-111 26.71793621305225 34.0 25.0 35.0 2.0 35.0 112-113 26.62680409085732 34.0 25.0 35.0 2.0 35.0 114-115 26.506246467048598 34.0 25.0 35.0 2.0 35.0 116-117 26.39170988056143 34.0 25.0 35.0 2.0 35.0 118-119 26.246605754492137 34.0 25.0 35.0 2.0 35.0 120-121 26.07136692436532 34.0 24.0 35.0 2.0 35.0 122-123 25.838010325360663 34.0 23.5 35.0 2.0 35.0 124-125 24.383932685586394 32.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.91737895196691 1101 2 1.067499388678435 1101 3 0.9906870627646533 1101 4 1.057662807343604 1101 5 1.0152879235518029 1101 6 1.0178431738648754 1101 7 1.1983568574403591 1101 8 1.0288823030880394 1101 9 1.4414250284965249 1101 10-11 1.5198146935249568 1101 12-13 1.2156143482987432 1101 14-15 1.3633667994038703 1101 16-17 1.2092834145055917 1101 18-19 1.6267637632919474 1101 20-21 1.9980469466817041 1101 22-23 1.8719130725140474 1101 24-25 2.105387032172338 1101 26-27 2.182726952098051 1101 28-29 1.8920539169907684 1101 30-31 1.989915385036035 1101 32-33 1.9632916864947916 1101 34-35 2.2492938019352877 1101 36-37 2.2142104532796907 1101 38-39 2.131949583451828 1101 40-41 1.8905826743744711 1101 42-43 1.8130033969426904 1101 44-45 1.657063053608617 1101 46-47 1.5479246677309106 1101 48-49 1.465341796243365 1101 50-51 1.3955430641039044 1101 52-53 1.2326796344487718 1101 54-55 1.2782157663353253 1101 56-57 1.3077995930245727 1101 58-59 1.2729948461410956 1101 60-61 1.3077299411840073 1101 62-63 1.2193408324465196 1101 64-65 1.3197939456356593 1101 66-67 1.2189494670051424 1101 68-69 1.04750539294254 1101 70-71 1.0690602764739197 1101 72-73 0.8540635572038582 1101 74-75 0.8941214263371791 1101 76-77 1.0055611526230912 1101 78-79 1.0357179865345252 1101 80-81 1.1130973485952822 1101 82-83 0.9482226317728646 1101 84-85 1.0796969362627955 1101 86-87 1.0358247590147194 1101 88-89 1.0379620575384294 1101 90-91 1.0860702015368382 1101 92-93 0.8975798004424824 1101 94-95 1.0203030204778756 1101 96-97 0.9420978912618025 1101 98-99 0.9934982267948804 1101 100-101 1.0811097907204683 1101 102-103 1.0106983580848556 1101 104-105 0.9895113785037601 1101 106-107 1.073563765336381 1101 108-109 1.0950553288858664 1101 110-111 0.8395081499732058 1101 112-113 0.8770453436208747 1101 114-115 1.1495334959306511 1101 116-117 1.2601433716903934 1101 118-119 1.1393475595563451 1101 120-121 1.2299864201292117 1101 122-123 1.2438324319974967 1101 124-125 1.5292007725875756 1102 1 -0.1702055259053381 1102 2 0.22227014518073318 1102 3 0.3352272619222987 1102 4 0.4347217547242437 1102 5 0.48401898778441677 1102 6 -0.24402724683969979 1102 7 -0.8790915191504531 1102 8 0.03164034553068973 1102 9 0.19113531902662828 1102 10-11 0.28020548217298114 1102 12-13 0.3216086403175922 1102 14-15 0.6249376671479752 1102 16-17 0.4183497252316428 1102 18-19 0.621693536721736 1102 20-21 0.9409195756438606 1102 22-23 0.8959655899194061 1102 24-25 0.8957836813237989 1102 26-27 0.465564836646962 1102 28-29 0.9109946579341326 1102 30-31 0.8156797994291693 1102 32-33 0.8574412130539741 1102 34-35 1.1935275266043632 1102 36-37 1.3126297130326208 1102 38-39 1.2990575493432033 1102 40-41 1.0477728572437286 1102 42-43 0.907911017337895 1102 44-45 0.992781205668372 1102 46-47 0.48933621184507814 1102 48-49 0.1765123344066808 1102 50-51 0.5844837884687664 1102 52-53 0.3462970364138229 1102 54-55 0.2549909310635492 1102 56-57 0.24798546713896386 1102 58-59 0.4469599947964511 1102 60-61 0.40819747590148125 1102 62-63 0.2615510670241541 1102 64-65 0.24738139520590607 1102 66-67 0.33293356489792814 1102 68-69 0.03762625857288526 1102 70-71 0.04640822873776074 1102 72-73 0.23960769707343132 1102 74-75 -0.15610410357418303 1102 76-77 0.005694091553664293 1102 78-79 -0.03423690333857721 1102 80-81 -0.2379104383310704 1102 82-83 -0.0417428103221269 1102 84-85 -0.39858594037402284 1102 86-87 0.03665990147800713 1102 88-89 0.21960678669115907 1102 90-91 -0.20745719569504928 1102 92-93 0.1868453807193582 1102 94-95 0.105730012448074 1102 96-97 0.18696798205401421 1102 98-99 0.23270468395891086 1102 100-101 0.24679459510647916 1102 102-103 0.07925464337117916 1102 104-105 0.4145592075063753 1102 106-107 0.34836024339762517 1102 108-109 -0.19738234213183148 1102 110-111 0.11905903398082529 1102 112-113 -0.0929323209809354 1102 114-115 -0.014305498549532558 1102 116-117 -0.023205959905510554 1102 118-119 0.30921543297278475 1102 120-121 0.6446427351739601 1102 122-123 0.547369830215807 1102 124-125 0.8170483721435993 1103 1 0.8850790049610815 1103 2 0.9425754040809977 1103 3 0.7257334377014324 1103 4 0.8945581347891434 1103 5 0.8588075223275027 1103 6 1.0229864293995377 1103 7 1.0385106211347193 1103 8 1.1006183759983514 1103 9 1.2850633100346016 1103 10-11 1.3874488564286338 1103 12-13 1.282276481243585 1103 14-15 1.575678927008994 1103 16-17 1.3767425253722152 1103 18-19 1.4284199318738615 1103 20-21 1.7380678723266527 1103 22-23 1.8003468630480342 1103 24-25 1.8337876761623306 1103 26-27 1.8876995381934307 1103 28-29 1.7560902190051522 1103 30-31 1.723189981484417 1103 32-33 1.810082516689775 1103 34-35 2.0754338818496976 1103 36-37 1.8269359555656877 1103 38-39 2.009991106298763 1103 40-41 1.7032690355287343 1103 42-43 1.5271674491631018 1103 44-45 1.386420917399498 1103 46-47 1.364547105422453 1103 48-49 1.2805906673466296 1103 50-51 1.3272311601623912 1103 52-53 1.2579882297808496 1103 54-55 1.1272870586072408 1103 56-57 1.0571538399129246 1103 58-59 1.028767986761025 1103 60-61 0.8893140135139959 1103 62-63 0.9114350794557744 1103 64-65 1.0488007482333828 1103 66-67 1.1317108864429208 1103 68-69 1.1297956680084837 1103 70-71 1.1245614945338502 1103 72-73 1.1501147358707797 1103 74-75 1.1574451784050623 1103 76-77 1.1984133371717647 1103 78-79 1.2211641349867115 1103 80-81 1.2263230715224154 1103 82-83 1.2707447512978938 1103 84-85 1.2189153931450853 1103 86-87 1.188941440894542 1103 88-89 1.2308667828855597 1103 90-91 1.2374388314058002 1103 92-93 1.3181187692927878 1103 94-95 1.3220521303571573 1103 96-97 1.3765401225783336 1103 98-99 1.1952127292642452 1103 100-101 1.1322739497352146 1103 102-103 1.0752439481840135 1103 104-105 1.1922652115136891 1103 106-107 1.2017461861826355 1103 108-109 1.2526799407489015 1103 110-111 1.3693564113412613 1103 112-113 1.354072018025093 1103 114-115 1.4620599678286297 1103 116-117 1.3712046454445783 1103 118-119 1.388870662395263 1103 120-121 1.3507862291083974 1103 122-123 1.2920118908121623 1103 124-125 1.2733301569484503 1104 1 0.3746371856568622 1104 2 0.4733432726047617 1104 3 0.4024423496267211 1104 4 0.40880452866756656 1104 5 0.43481647097184606 1104 6 0.454800040663784 1104 7 0.4549436498766326 1104 8 0.453608577913144 1104 9 0.736112004306932 1104 10-11 0.5615331061542363 1104 12-13 0.5725039593046972 1104 14-15 0.566499813048857 1104 16-17 0.6163577356663623 1104 18-19 0.842176821699681 1104 20-21 1.0873384437532252 1104 22-23 0.8711740723696764 1104 24-25 1.3411831143947737 1104 26-27 1.3918907134133747 1104 28-29 1.2491060442184292 1104 30-31 1.2848187167980427 1104 32-33 1.188019716096278 1104 34-35 1.600010887315527 1104 36-37 1.5647317215682506 1104 38-39 1.5287774063885067 1104 40-41 1.1738434636569366 1104 42-43 1.16146409912443 1104 44-45 1.150404474865816 1104 46-47 1.0647418064722984 1104 48-49 1.060239405041493 1104 50-51 0.8479288022386271 1104 52-53 0.9390386449463222 1104 54-55 0.7581847945321876 1104 56-57 0.7458303630284213 1104 58-59 0.6556842563049159 1104 60-61 0.6462561164654304 1104 62-63 0.7598465550115883 1104 64-65 0.7614387573601178 1104 66-67 0.7688961856131904 1104 68-69 0.7776483035263162 1104 70-71 0.7345408738317047 1104 72-73 0.7205953653174646 1104 74-75 0.7593491165489574 1104 76-77 0.7596391133495999 1104 78-79 0.7025033000133583 1104 80-81 0.7063352978678878 1104 82-83 0.6978563222031937 1104 84-85 0.7742582660961439 1104 86-87 0.7887813578114233 1104 88-89 0.8350449013290309 1104 90-91 0.8733236056036127 1104 92-93 0.8798988331459299 1104 94-95 0.8272564814153576 1104 96-97 0.628963330223101 1104 98-99 0.7232703411648238 1104 100-101 0.7581637365345664 1104 102-103 0.7106694840371226 1104 104-105 0.7243063408598189 1104 106-107 0.7320699242026656 1104 108-109 0.8474282651292562 1104 110-111 0.9177820442447384 1104 112-113 0.9023764380190684 1104 114-115 0.8099200572150203 1104 116-117 0.9098993780107811 1104 118-119 0.836956018695183 1104 120-121 0.8914619398022445 1104 122-123 0.9093299788998763 1104 124-125 1.2259994423075504 1105 1 6.070332215166729E-4 1105 2 0.24965471913594328 1105 3 0.27369279400046764 1105 4 0.3696186631010363 1105 5 0.40149912085222894 1105 6 0.37490804956537005 1105 7 0.421191215880917 1105 8 0.21624891208617214 1105 9 0.21653005046139384 1105 10-11 0.43402074267807933 1105 12-13 0.30303070191050807 1105 14-15 0.5412254538661045 1105 16-17 0.5720371550564138 1105 18-19 0.691743665347488 1105 20-21 0.9895638992698395 1105 22-23 1.1450661286462207 1105 24-25 1.2366405764260335 1105 26-27 0.9703370615802243 1105 28-29 0.7635745453976952 1105 30-31 0.7301589575557088 1105 32-33 0.815468143405667 1105 34-35 1.2852235299647177 1105 36-37 1.4799124726388655 1105 38-39 1.5797106856451961 1105 40-41 1.1293264047722715 1105 42-43 0.8145676709454648 1105 44-45 0.9529801733994887 1105 46-47 0.9524786410368975 1105 48-49 0.8188442592620646 1105 50-51 0.7253002279485798 1105 52-53 0.7722768771644652 1105 54-55 0.59844651651051 1105 56-57 0.49749990673979383 1105 58-59 0.5199457374421073 1105 60-61 0.5647296546422247 1105 62-63 0.5344224848481289 1105 64-65 0.5592235654115285 1105 66-67 0.43693662705193503 1105 68-69 0.31217134965792326 1105 70-71 0.4959464997772116 1105 72-73 0.4705520457079686 1105 74-75 0.5023774414134401 1105 76-77 0.5595491158673056 1105 78-79 0.670789619978752 1105 80-81 0.6181194835476589 1105 82-83 0.5701575651779152 1105 84-85 0.5966397695767185 1105 86-87 0.5380186445492825 1105 88-89 0.7524598910664579 1105 90-91 0.5735806717174832 1105 92-93 0.5806884025788186 1105 94-95 0.7185976366539606 1105 96-97 0.7875917916437096 1105 98-99 0.6833017915987476 1105 100-101 0.6911788528924276 1105 102-103 0.6767874389620765 1105 104-105 0.8652105244411707 1105 106-107 0.7833415633908594 1105 108-109 0.759965526451623 1105 110-111 0.8704924309954549 1105 112-113 0.922119896509642 1105 114-115 0.8729639493189865 1105 116-117 0.9774506953541433 1105 118-119 0.9831271116787335 1105 120-121 0.8762082707008751 1105 122-123 1.0483716179867244 1105 124-125 1.171358362162895 1106 1 0.2765520785818971 1106 2 0.5918077075198696 1106 3 0.3256427519404319 1106 4 0.4803239621760369 1106 5 0.32134701120077835 1106 6 0.38631076604809067 1106 7 0.13742824600993941 1106 8 0.41432383932761496 1106 9 0.5741929321327603 1106 10-11 0.5917129358295128 1106 12-13 0.5589501624487241 1106 14-15 0.7379351127433011 1106 16-17 0.6073200077793857 1106 18-19 0.6527509318204565 1106 20-21 1.0656399895875523 1106 22-23 1.1185056339940829 1106 24-25 1.188667556042212 1106 26-27 1.3671093515635704 1106 28-29 1.1958667602522794 1106 30-31 1.0025098280067226 1106 32-33 0.9107438141021049 1106 34-35 1.271481759412616 1106 36-37 1.2517604380509084 1106 38-39 1.4134252517985786 1106 40-41 1.1255608382753906 1106 42-43 1.0834038154883814 1106 44-45 0.9750196221233693 1106 46-47 0.7474388206671136 1106 48-49 0.9064230147419039 1106 50-51 0.8907140685153365 1106 52-53 0.7459440207291195 1106 54-55 0.6144781826998731 1106 56-57 0.7178491111075154 1106 58-59 0.6511922749342887 1106 60-61 0.7628161065783345 1106 62-63 0.7430571940464255 1106 64-65 0.74922146648926 1106 66-67 0.5551627582874055 1106 68-69 0.6128147335856866 1106 70-71 0.784957030288485 1106 72-73 0.7880357622323011 1106 74-75 0.7528450584492639 1106 76-77 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 101977.0 3 18680.0 4 11807.0 5 9973.0 6 9703.0 7 10032.0 8 10416.0 9 11094.0 10 12175.0 11 13305.0 12 14138.0 13 16246.0 14 19296.0 15 17052.0 16 11888.0 17 10677.0 18 9768.0 19 9007.0 20 8640.0 21 9171.0 22 11280.0 23 14908.0 24 20101.0 25 24576.0 26 30642.0 27 33724.0 28 38579.0 29 46085.0 30 52225.0 31 59836.0 32 74748.0 33 94198.0 34 123110.0 35 172280.0 36 261619.0 37 376890.0 38 143961.0 39 315.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75507470063838 20.368920939690874 13.752922034833476 22.12308232483727 2 14.941900590737845 21.846433238055283 40.547645904201936 22.66402026700494 3 17.63254647753984 28.931566482357002 29.369656774411972 24.066230265691182 4 11.401232819884784 18.122128375024687 39.26810331946002 31.208535485630513 5 12.46489444690891 40.215249617137204 33.89951569358645 13.420340242367432 6 28.866680633173868 38.816301944043765 18.93429977383662 13.382717648945746 7 27.522011632256838 34.26500061651379 22.02479848358506 16.18818926764431 8 25.880586503890555 35.06500630576316 20.79585313788776 18.258554052458518 9 27.436210502338216 16.887113264986787 20.58300928545418 35.09366694722081 10-11 23.819066914230127 28.29218827222089 28.014515271231407 19.874229542317572 12-13 24.885348541478802 26.25037746948636 28.018005686336178 20.846268302698657 14-15 22.36229441810035 27.349897453984923 26.21483716737861 24.072970960536114 16-17 21.418371594314536 30.692852251042762 26.50891943310497 21.37985672153773 18-19 21.08259890403129 28.926531120845738 29.292385193529096 20.69848478159387 20-21 22.663056696370376 28.075216886886523 28.79602666411001 20.46569975263309 22-23 22.0032407069997 28.03919463493982 28.55421784311436 21.403346814946122 24-25 21.629512668742183 28.272454697951073 29.01209491492126 21.085937718385487 26-27 21.541917470077983 28.588780292684607 28.681738341641992 21.187563895595414 28-29 21.193697441345503 27.961119297386805 29.422737390167708 21.422445871099985 30-31 21.388373267909692 28.183355110446794 29.127537956698983 21.300733664944524 32-33 21.445568728073923 28.329883678311894 28.711135214721107 21.513412378893072 34-35 21.781749889169312 28.079466842358663 28.840012215540018 21.298771052932004 36-37 22.175204155447336 27.62924184717085 28.586961377465066 21.608592619916752 38-39 21.496278711597274 28.087525246562134 28.65825689271635 21.757939149124244 40-41 21.637686040414106 27.94226884716148 29.07015784122966 21.349887271194753 42-43 21.82114285848638 28.055538647501383 28.27837664218932 21.84494185182292 44-45 21.51912326178885 28.272210410482483 28.25551605923438 21.953150268494284 46-47 21.652415921824485 28.27801961493209 27.92170745622376 22.147857007019663 48-49 21.871981417879542 28.286366361972494 27.782308055082716 22.059344165065248 50-51 21.979090148200704 28.351965960754978 27.352893847836967 22.316050043207355 52-53 22.041802978075587 28.51513644375855 27.398723801304204 22.044336776861662 54-55 21.86007140253867 28.291890860971446 27.597564528117523 22.25047320837236 56-57 21.962654297686747 28.773134040617325 27.225487719532893 22.03872394216304 58-59 22.007667242911484 27.975249161885092 27.59904436353682 22.41803923166661 60-61 22.159687573469867 28.492594237350122 27.459705859303575 21.888012329876442 62-63 21.847375770003367 28.369359143992455 27.765009709306092 22.018255376698086 64-65 21.724092075379815 27.898321743399197 27.819943708895618 22.55764247232537 66-67 21.951158348107594 28.52495724455072 27.206728442097745 22.31715596524394 68-69 21.872344872632265 28.277622468974528 27.6365774003919 22.213455258001304 70-71 21.772227422016364 28.166110827103374 27.91666873468415 22.144993016196103 72-73 21.929330255851593 28.341987117943575 27.40016798138881 22.328514644816025 74-75 21.730067362477417 28.66959368316126 27.181444024989005 22.418894929372318 76-77 21.808832591828637 28.438174547880667 27.61143011791714 22.14156274237355 78-79 21.857978310455763 28.370771254614436 27.46251244709007 22.308737987839727 80-81 21.609912361429625 29.10606777393225 27.21322720957005 22.07079265506808 82-83 22.23677222866132 28.71635768147607 26.950007797445103 22.096862292417512 84-85 21.830269961893755 28.369403831103764 27.086361266418756 22.713964940583725 86-87 21.899314870065123 28.27506972902271 27.38484751829464 22.44076788261752 88-89 22.091973668363188 28.098078214795073 27.43665220257187 22.373295914269868 90-91 21.846794886700767 28.48730649879776 27.549941462040728 22.115957152460748 92-93 21.98905352602165 28.367678644292365 27.377861811737574 22.265406017948415 94-95 21.800834316177927 27.977940734601653 27.46771505745787 22.75350989176255 96-97 22.267484026577435 28.1117867869437 27.225175618819904 22.395553567658965 98-99 21.766415330422483 28.16920723942422 27.193005599229924 22.87137183092337 100-101 21.57344446728822 28.211178199827426 27.506489794884082 22.708887538000273 102-103 22.219926420572982 27.701290722325954 27.518961696276413 22.55982116082465 104-105 21.678187780174905 27.882479156593366 27.696699405384635 22.74263365784709 106-107 21.747626783357273 28.154430113239936 27.56196511036525 22.535977993037537 108-109 21.976577250561878 28.226831936522334 26.82219315174268 22.974397661173114 110-111 21.48601894094245 28.376462659490127 27.208816641698547 22.92870175786888 112-113 21.604840234843962 28.107665028665885 27.594479348756245 22.693015387733904 114-115 21.28020053058269 28.446619389986637 27.36231023936823 22.910869840062443 116-117 21.354647195946757 28.553509128467258 27.36288491538157 22.728958760204417 118-119 21.30451977460162 28.38954361320752 27.279818109817448 23.026118502373414 120-121 21.115801979080736 28.54696751145826 27.394192948094716 22.94303756136629 122-123 21.29239933504761 28.56664831186309 27.258163272769448 22.882789080319853 124-125 21.411308801267484 28.4534305823287 27.103724539677582 23.03153607672624 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2159.0 1 2059.5 2 2531.0 3 2648.5 4 1793.0 5 1186.5 6 898.0 7 940.0 8 1245.5 9 1397.0 10 1241.5 11 1110.0 12 1145.5 13 1235.5 14 1375.5 15 1571.5 16 1729.0 17 1881.5 18 2048.0 19 2271.0 20 2582.5 21 2976.5 22 3658.5 23 4595.0 24 5515.5 25 6944.0 26 9705.0 27 12777.0 28 15197.5 29 18596.5 30 23976.0 31 29444.0 32 37052.0 33 46166.5 34 54305.5 35 59486.0 36 66067.5 37 72640.0 38 72062.0 39 70412.0 40 67782.0 41 66986.0 42 67255.5 43 67280.5 44 68108.0 45 67327.5 46 63235.5 47 58543.5 48 60432.0 49 60932.5 50 57077.0 51 53062.0 52 51117.0 53 51019.5 54 49952.0 55 47112.0 56 42648.5 57 38557.0 58 35657.5 59 33013.5 60 30338.5 61 26311.5 62 21883.5 63 17554.0 64 13871.5 65 11690.0 66 9096.0 67 7333.0 68 5458.5 69 3446.5 70 2650.0 71 2295.5 72 2100.0 73 1915.0 74 1514.0 75 1239.5 76 878.5 77 541.0 78 382.0 79 264.5 80 195.0 81 133.5 82 92.5 83 66.0 84 48.5 85 38.0 86 32.5 87 24.5 88 20.0 89 18.0 90 11.0 91 5.0 92 4.5 93 4.0 94 2.0 95 2.5 96 4.0 97 2.0 98 0.5 99 1.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011284547170974473 2 0.0039182455454772475 3 0.0069483554339796515 4 0.007105085255798741 5 0.013113061758863854 6 9.92622204854236E-4 7 0.007000598707919349 8 0.0 9 0.0027688935188039214 10-11 0.0 12-13 0.0031345964363817982 14-15 0.01812841605707473 16-17 0.030066004152295413 18-19 0.005616151948517388 20-21 0.008541775289140399 22-23 0.0017501496769798373 24-25 0.011415155355823714 26-27 0.018572483885562152 28-29 5.485543763668146E-4 30-31 0.0017240280400099889 32-33 0.008489532015200703 34-35 0.008698505110959489 36-37 0.010474776424909175 38-39 0.0 40-41 0.023953541101350905 42-43 0.009168694576416758 44-45 0.015725225455848688 46-47 0.0036570291757787644 48-49 0.023744568005592118 50-51 0.0050153542982108775 52-53 0.0 54-55 0.016952942393431557 56-57 0.0045190431957837585 58-59 2.612163696984832E-4 60-61 0.0037615157236581577 62-63 0.02580817732621014 64-65 0.01674396929767277 66-67 0.0020113660466783206 68-69 0.019434497905567148 70-71 0.00263828533395468 72-73 0.01146739862976341 74-75 0.0 76-77 0.0056683952224570846 78-79 0.0010448654787939327 80-81 0.0035264209909295226 82-83 0.0017501496769798373 84-85 0.0 86-87 0.005511665400637995 88-89 0.01580359036675823 90-91 0.02123689085648668 92-93 0.004231705189115427 94-95 0.023979662738320754 96-97 0.01661336111282353 98-99 0.0010187438418240843 100-101 0.008123829097622827 102-103 0.0 104-105 0.0014366900333416574 106-107 2.612163696984832E-5 108-109 0.0 110-111 7.575274721256011E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 5.224327393969664E-5 122-123 0.0 124-125 0.021367499041335922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1914122.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.078878732652385 #Duplication Level Percentage of deduplicated Percentage of total 1 76.42318865700075 33.68648465174551 2 11.2929353715078 9.95559857552746 3 4.411112731761564 5.833107095381309 4 2.276033208090171 4.012999670835857 5 1.3126275283579136 2.8929574821814845 6 0.8507579228663055 2.2500273187720268 7 0.5697147443410608 1.7578670989609568 8 0.42690668917715985 1.5054054545918523 9 0.31196833360812143 1.2376092910986025 >10 1.7613827487711218 14.75083653549236 >50 0.20190210396964528 6.254674591057144 >100 0.1536056648543982 12.692486546530384 >500 0.006389740251432728 1.9089545633782192 >1k 0.0014745554426383218 1.2609911244469505 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4324 0.22589991651524827 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2968 0.15505803705301963 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2794 0.1459677073875124 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.6121636969848316E-5 0.0 16-17 0.0 0.0 0.0 5.224327393969663E-5 5.224327393969663E-5 18-19 0.0 0.0 0.0 2.0897309575878653E-4 5.224327393969663E-5 20-21 0.0 0.0 0.0 4.1794619151757305E-4 5.224327393969663E-5 22-23 0.0 0.0 0.0 9.14257293944691E-4 5.224327393969663E-5 24-25 0.0 0.0 0.0 0.001645663129100444 5.224327393969663E-5 26-27 0.0 0.0 0.0 0.002403190601226045 5.224327393969663E-5 28-29 0.0 0.0 0.0 0.0038398806345677026 5.224327393969663E-5 30-31 0.0 0.0 0.0 0.008515653652170552 5.224327393969663E-5 32-33 0.0 0.0 0.0 0.014105683963718092 5.224327393969663E-5 34-35 0.0 0.0 0.0 0.02113240430860729 5.224327393969663E-5 36-37 0.0 0.0 0.0 0.03343569532140585 5.224327393969663E-5 38-39 0.0 0.0 0.0 0.0535754774251589 5.224327393969663E-5 40-41 0.0 0.0 0.0 0.08210030499623326 5.224327393969663E-5 42-43 0.0 0.0 0.0 0.11005045655397097 5.224327393969663E-5 44-45 0.0 0.0 0.0 0.14076950163051258 5.224327393969663E-5 46-47 0.0 0.0 0.0 0.17995195708528505 5.224327393969663E-5 48-49 0.0 0.0 0.0 0.2247244428516051 5.224327393969663E-5 50-51 0.0 0.0 0.0 0.2793186641185881 5.224327393969663E-5 52-53 0.0 0.0 0.0 0.3325023169891992 5.224327393969663E-5 54-55 0.0 0.0 0.0 0.38495456402465467 5.224327393969663E-5 56-57 0.0 0.0 0.0 0.44634041090379817 5.224327393969663E-5 58-59 0.0 0.0 0.0 0.5186451020363383 5.224327393969663E-5 60-61 0.0 0.0 0.0 0.6030702327228881 5.224327393969663E-5 62-63 0.0 5.224327393969663E-5 0.0 0.6904732300240006 5.224327393969663E-5 64-65 0.0 5.224327393969663E-5 0.0 0.7786076331602687 5.224327393969663E-5 66-67 0.0 5.224327393969663E-5 0.0 0.8810828149929837 5.224327393969663E-5 68-69 0.0 5.224327393969663E-5 0.0 0.9999885064797334 5.224327393969663E-5 70-71 0.0 5.224327393969663E-5 0.0 1.1392951964399343 5.224327393969663E-5 72-73 0.0 5.224327393969663E-5 0.0 1.2879534324353412 5.224327393969663E-5 74-75 0.0 5.224327393969663E-5 0.0 1.4317269223173863 7.836491090954495E-5 76-77 0.0 5.224327393969663E-5 0.0 1.587568608479501 1.0448654787939326E-4 78-79 0.0 5.224327393969663E-5 0.0 1.7696364181593442 1.0448654787939326E-4 80-81 0.0 5.224327393969663E-5 0.0 1.9775907700763065 1.0448654787939326E-4 82-83 0.0 5.224327393969663E-5 0.0 2.2089239870812833 1.0448654787939326E-4 84-85 0.0 5.224327393969663E-5 0.0 2.4355553094316873 1.0448654787939326E-4 86-87 0.0 5.224327393969663E-5 0.0 2.667907270278488 1.0448654787939326E-4 88-89 0.0 5.224327393969663E-5 0.0 2.9254404891642225 1.0448654787939326E-4 90-91 0.0 5.224327393969663E-5 0.0 3.2293657353084075 1.0448654787939326E-4 92-93 0.0 5.224327393969663E-5 0.0 3.5702008544909885 1.0448654787939326E-4 94-95 0.0 5.224327393969663E-5 0.0 3.911140460221449 1.0448654787939326E-4 96-97 0.0 5.224327393969663E-5 0.0 4.257278271708909 1.0448654787939326E-4 98-99 0.0 5.224327393969663E-5 0.0 4.625514988072861 1.0448654787939326E-4 100-101 0.0 5.224327393969663E-5 0.0 5.046648019300755 1.0448654787939326E-4 102-103 0.0 5.224327393969663E-5 0.0 5.511221332809507 1.0448654787939326E-4 104-105 0.0 5.224327393969663E-5 0.0 5.985041705805585 1.0448654787939326E-4 106-107 0.0 5.224327393969663E-5 0.0 6.452827980661629 1.0448654787939326E-4 108-109 0.0 5.224327393969663E-5 0.0 6.938141873924442 1.0448654787939326E-4 110-111 0.0 5.224327393969663E-5 0.0 7.485573019901553 1.0448654787939326E-4 112-113 0.0 5.224327393969663E-5 0.0 8.082426303025617 1.0448654787939326E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8460 0.0 51.62468 1 GGTATCA 4590 0.0 49.64983 1 ATCAACG 10390 0.0 41.405155 3 TCAACGC 10405 0.0 41.28828 4 CAACGCA 10460 0.0 41.241833 5 AACGCAG 10700 0.0 40.089108 6 TATCAAC 11305 0.0 38.63288 2 GTACATG 13405 0.0 38.129227 1 TACATGG 14050 0.0 35.87061 2 ACATGGG 14275 0.0 34.80503 3 ACGCAGA 12340 0.0 34.520157 7 CGCAGAG 12250 0.0 33.852776 8 CATGGGA 9400 0.0 30.700504 4 GCAGAGT 12960 0.0 30.620935 9 ATGGGAT 3645 0.0 30.526411 5 CATGGGG 4415 0.0 30.054245 4 GTATAGG 710 0.0 29.331978 1 GAGTACT 4790 0.0 27.95345 12-13 ATGGGTA 1110 0.0 25.194548 5 CATGGGT 1765 0.0 24.609898 4 >>END_MODULE