FastQCFastQC Report
Thu 26 May 2016
SRR1364520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364520_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914122
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91610.4786006325615609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76290.3985639368859456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48420.2529619324160111No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66900.052.837771
GTATCAA116100.049.7516441
ATCAACG149650.038.2441443
TCAACGC150000.038.1549074
CAACGCA151150.037.947095
AACGCAG154550.037.1892786
TATCAAC154850.036.767572
GTACATG117150.034.8040471
ACGCAGA175950.032.2265057
CGCAGAG176050.032.0054248
TACATGG125900.031.3148632
ACATGGG124800.030.875073
GCAGAGT182900.029.8625769
GAGTACT104650.028.99540912-13
CATGGGA82150.028.2732224
TACCGTA1107.753895E-427.0449987
AGTACTT110950.026.35690912-13
GTACTTT116550.026.16253714-15
CAGAGTA182100.024.76512510-11
AGAGTAC178550.024.44114510-11
Produced by FastQC (version 0.11.5)