##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364520_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1914122 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.74448859581573 31.0 30.0 34.0 26.0 34.0 2 29.643529513792746 31.0 30.0 34.0 25.0 34.0 3 29.90909513604671 31.0 30.0 34.0 26.0 34.0 4 33.43333601515473 35.0 33.0 37.0 28.0 37.0 5 32.87216959002613 35.0 33.0 37.0 26.0 37.0 6 32.87409632196903 35.0 33.0 37.0 26.0 37.0 7 32.6500531314096 35.0 33.0 37.0 25.0 37.0 8 32.5603681479028 35.0 33.0 37.0 24.0 37.0 9 33.73864832022201 38.0 33.0 39.0 22.0 39.0 10-11 33.67659036362363 38.0 33.0 39.0 22.0 39.0 12-13 33.56718040960816 38.0 33.0 39.0 20.5 39.0 14-15 34.46132404308607 38.0 33.0 41.0 19.0 41.0 16-17 34.48281091800836 38.5 33.0 41.0 20.0 41.0 18-19 34.52588549737164 39.0 33.5 41.0 20.0 41.0 20-21 34.56444077232277 39.0 34.0 41.0 21.0 41.0 22-23 34.517763758004975 39.0 33.0 41.0 22.0 41.0 24-25 34.47479314275684 39.0 33.0 41.0 22.0 41.0 26-27 34.34300634964752 39.0 33.0 41.0 21.0 41.0 28-29 34.18078758825195 38.5 33.0 41.0 19.5 41.0 30-31 33.97282722835848 38.0 33.0 40.0 18.5 41.0 32-33 33.713488220709024 38.0 33.0 40.0 17.5 41.0 34-35 33.496341664742374 38.0 32.0 40.0 16.0 41.0 36-37 33.25466010003542 38.0 32.0 40.0 15.0 41.0 38-39 33.0606001080391 38.0 32.0 40.0 11.0 41.0 40-41 33.06087177306358 38.0 32.0 40.0 9.0 41.0 42-43 32.88254353693234 38.0 32.0 40.0 8.5 41.0 44-45 32.66714948158999 38.0 31.0 40.0 4.5 41.0 46-47 32.34430224405759 38.0 31.0 40.0 2.0 41.0 48-49 32.003319015193384 38.0 30.0 40.0 2.0 41.0 50-51 31.820919460724028 37.5 30.0 40.0 2.0 41.0 52-53 31.639177387857202 37.0 30.0 40.0 2.0 41.0 54-55 31.44993474815085 37.0 30.0 40.0 2.0 41.0 56-57 31.241944609591236 37.0 30.0 40.0 2.0 41.0 58-59 31.05063679326605 36.0 29.0 40.0 2.0 41.0 60-61 30.827750791224382 36.0 29.0 40.0 2.0 41.0 62-63 30.67368250299615 36.0 29.0 40.0 2.0 41.0 64-65 30.551600420453866 35.5 29.0 39.0 2.0 41.0 66-67 30.260009550070478 35.0 29.0 39.0 2.0 41.0 68-69 29.984245518310743 35.0 29.0 39.0 2.0 41.0 70-71 29.686401650469513 35.0 28.0 38.5 2.0 40.0 72-73 29.406483494782464 35.0 28.0 37.5 2.0 40.0 74-75 29.059757424030444 35.0 28.0 37.0 2.0 39.0 76-77 28.74875869981119 34.5 28.0 36.5 2.0 39.0 78-79 28.459521388918784 34.0 27.5 36.0 2.0 39.0 80-81 28.176083342650053 34.0 27.0 36.0 2.0 37.5 82-83 27.878658204649444 34.0 26.5 35.0 2.0 37.0 84-85 27.636305575088734 34.0 26.0 35.0 2.0 37.0 86-87 27.41086853398059 34.0 26.0 35.0 2.0 36.0 88-89 27.22671099334316 34.0 26.0 35.0 2.0 36.0 90-91 27.067045099528663 34.0 26.0 35.0 2.0 36.0 92-93 26.958636649074613 34.0 26.0 35.0 2.0 35.0 94-95 26.83149898491319 34.0 25.0 35.0 2.0 35.0 96-97 26.710056098827557 34.0 25.0 35.0 2.0 35.0 98-99 26.622318222140493 34.0 25.0 35.0 2.0 35.0 100-101 26.522743064444168 34.0 25.0 35.0 2.0 35.0 102-103 25.27775005981855 31.5 21.5 34.0 2.0 35.0 104-105 26.186899528870157 33.0 24.0 35.0 2.0 35.0 106-107 26.3476288345257 34.0 25.0 35.0 2.0 35.0 108-109 26.34768081658327 34.0 25.0 35.0 2.0 35.0 110-111 26.25809248313326 34.0 24.0 35.0 2.0 35.0 112-113 26.178349133440815 34.0 24.0 35.0 2.0 35.0 114-115 26.070752021031055 34.0 24.0 35.0 2.0 35.0 116-117 25.948977651372275 34.0 23.5 35.0 2.0 35.0 118-119 25.81287869843197 33.5 23.0 35.0 2.0 35.0 120-121 25.61780570935395 33.0 19.5 35.0 2.0 35.0 122-123 25.316236896080817 33.0 16.5 35.0 2.0 35.0 124-125 23.556435796673355 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1359454817068695 1101 2 1.2664223296428396 1101 3 1.0852780293623567 1101 4 1.1035697719311486 1101 5 1.1917549973425565 1101 6 1.2151282919233992 1101 7 1.2820162465932867 1101 8 1.436312671396152 1101 9 1.6781479621724529 1101 10-11 1.6880274657807348 1101 12-13 1.5360206302366208 1101 14-15 1.6951838514195927 1101 16-17 1.6459521410447735 1101 18-19 1.783390493680571 1101 20-21 1.8505341041562673 1101 22-23 1.8485598517754909 1101 24-25 1.8945599313551824 1101 26-27 1.7955099601536304 1101 28-29 1.9992128723522598 1101 30-31 1.9654169467002731 1101 32-33 2.017780997216576 1101 34-35 1.9940133012651344 1101 36-37 1.9991985105106025 1101 38-39 2.014524324167546 1101 40-41 1.831231324954338 1101 42-43 1.765134981074084 1101 44-45 1.7654951114259063 1101 46-47 1.8355194082389517 1101 48-49 1.8642582520973896 1101 50-51 1.7966859411175804 1101 52-53 1.8575353841889104 1101 54-55 1.84999534153118 1101 56-57 1.951948113362107 1101 58-59 1.908556047375967 1101 60-61 1.8885077189314785 1101 62-63 1.8924674571708522 1101 64-65 1.7378140884337299 1101 66-67 1.856519007910137 1101 68-69 1.9102767087034564 1101 70-71 1.8222420622280957 1101 72-73 1.7484983095687738 1101 74-75 1.8078032718279964 1101 76-77 1.8683625148969583 1101 78-79 1.769318342609747 1101 80-81 1.6405962596606223 1101 82-83 1.8294598218055107 1101 84-85 1.767841809633051 1101 86-87 1.7987217644273343 1101 88-89 1.7680293425229863 1101 90-91 1.8105764786447764 1101 92-93 1.7278137644842708 1101 94-95 1.7569581648774388 1101 96-97 1.7797429714890818 1101 98-99 1.7675622011081877 1101 100-101 1.7852409603478563 1101 102-103 1.6529494946680003 1101 104-105 1.8204627145421668 1101 106-107 1.8067010022094294 1101 108-109 1.8531436299169215 1101 110-111 1.720139749899559 1101 112-113 1.7698398654958822 1101 114-115 1.799345088054018 1101 116-117 1.9512334737947867 1101 118-119 2.0210873109167444 1101 120-121 1.8885304791758202 1101 122-123 1.7189256567496436 1101 124-125 1.9714680081401355 1102 1 0.7876083891962935 1102 2 0.19833770297845987 1102 3 0.6599161412026469 1102 4 0.72522018751296 1102 5 0.864475928789588 1102 6 0.7940192061536493 1102 7 0.7671105078590017 1102 8 0.7301763015428477 1102 9 1.1367159850441624 1102 10-11 1.2415497361082686 1102 12-13 1.255345723221339 1102 14-15 1.4832115350452497 1102 16-17 1.4100360001241157 1102 18-19 1.553668602337794 1102 20-21 1.3392561579684568 1102 22-23 1.5064179413840009 1102 24-25 1.4688270990244305 1102 26-27 1.551621628026382 1102 28-29 1.1633825044999924 1102 30-31 1.4378372475836585 1102 32-33 1.5688117246567472 1102 34-35 1.5928678505327412 1102 36-37 1.3170894794499333 1102 38-39 0.7075790058974505 1102 40-41 0.46604839352554706 1102 42-43 0.6706156086701753 1102 44-45 0.3695942724703798 1102 46-47 0.6049683841429143 1102 48-49 0.20940750428222898 1102 50-51 0.14842629097388027 1102 52-53 -0.08981510044239016 1102 54-55 0.005266250970233699 1102 56-57 0.03704716167271016 1102 58-59 0.10968000821421242 1102 60-61 9.262873605244692E-4 1102 62-63 0.0071442259528566865 1102 64-65 0.006827982054403492 1102 66-67 -0.06173411858970468 1102 68-69 0.1123889559951472 1102 70-71 -0.3526463022272459 1102 72-73 -0.18145934891557047 1102 74-75 -0.031178152779983037 1102 76-77 -0.2858616688393596 1102 78-79 -0.08386008941131706 1102 80-81 -0.04444903282989543 1102 82-83 -0.3625965227489871 1102 84-85 -0.2405132415489568 1102 86-87 -0.11113507184577998 1102 88-89 -0.12661857550622813 1102 90-91 -0.12334686315188392 1102 92-93 -0.016317442820884764 1102 94-95 0.2310189093686077 1102 96-97 0.014600574518308207 1102 98-99 -0.1684532450899887 1102 100-101 -0.04786213892177926 1102 102-103 -0.1865236900058278 1102 104-105 -0.08965326457222034 1102 106-107 0.01372649830063466 1102 108-109 0.12338972132585369 1102 110-111 0.1812271882793084 1102 112-113 0.3820451928344255 1102 114-115 0.21856132239457793 1102 116-117 0.13520064502841578 1102 118-119 0.23214894898962868 1102 120-121 0.43599143885604974 1102 122-123 0.3623348492021101 1102 124-125 0.6924224578537803 1103 1 0.7673384828493681 1103 2 0.3491151992066541 1103 3 0.6789241693515962 1103 4 0.7435784565271746 1103 5 0.8685662192108126 1103 6 0.9540878732896019 1103 7 1.1402479741830973 1103 8 1.0352711243693662 1103 9 1.2574650224669242 1103 10-11 1.4112352855441372 1103 12-13 1.4269818497876372 1103 14-15 1.456279128087452 1103 16-17 1.5269999748324778 1103 18-19 1.5586658417192893 1103 20-21 1.481086754771546 1103 22-23 1.6655873019498841 1103 24-25 1.5546494773986552 1103 26-27 1.6520966519673337 1103 28-29 1.6306227490148757 1103 30-31 1.6281948386606757 1103 32-33 1.6084758857978443 1103 34-35 1.5980098274267291 1103 36-37 1.6876178233595738 1103 38-39 1.6737111558213869 1103 40-41 1.6286518387358484 1103 42-43 1.5442711458678176 1103 44-45 1.5691198723980193 1103 46-47 1.5285768192621418 1103 48-49 1.4916799144217556 1103 50-51 1.6013689231378478 1103 52-53 1.5271811594654174 1103 54-55 1.6325932548101427 1103 56-57 1.6378069816242373 1103 58-59 1.6870687750443665 1103 60-61 1.664936244303199 1103 62-63 1.564718510505557 1103 64-65 1.5491994729235685 1103 66-67 1.5522242132272837 1103 68-69 1.5651359013785715 1103 70-71 1.5722521371654814 1103 72-73 1.5809196595700854 1103 74-75 1.6642107305056406 1103 76-77 1.6297596366888456 1103 78-79 1.5115745442374546 1103 80-81 1.520027317871591 1103 82-83 1.5537050074966814 1103 84-85 1.650131329078686 1103 86-87 1.6647067608305832 1103 88-89 1.6642667564074571 1103 90-91 1.7716182573951258 1103 92-93 1.6290496570532014 1103 94-95 1.660406967068134 1103 96-97 1.6519928662059833 1103 98-99 1.7326178852871124 1103 100-101 1.6371408920397812 1103 102-103 1.4154354653176924 1103 104-105 1.4235613119093884 1103 106-107 1.5631071007705302 1103 108-109 1.5698174497891273 1103 110-111 1.5401652495660763 1103 112-113 1.5145281066334633 1103 114-115 1.5301231150185828 1103 116-117 1.629322399222115 1103 118-119 1.5375718227155062 1103 120-121 1.5162453221780687 1103 122-123 1.5432110414409799 1103 124-125 1.8511197578545122 1104 1 0.458330007450634 1104 2 0.6634839565917368 1104 3 0.4018185515359498 1104 4 0.1920988386054745 1104 5 0.4708164870998459 1104 6 0.5379553004843132 1104 7 0.6721114113069646 1104 8 0.5167252990860476 1104 9 0.7222309359760359 1104 10-11 0.6825280364818695 1104 12-13 0.7014875682555228 1104 14-15 0.7528335398271437 1104 16-17 0.7618140433340344 1104 18-19 0.7409166298783418 1104 20-21 0.5960733828315057 1104 22-23 0.6526496373019341 1104 24-25 0.8863902508600816 1104 26-27 0.7843613473442375 1104 28-29 0.8892166092283276 1104 30-31 0.7900321322160337 1104 32-33 0.8105843801063912 1104 34-35 1.0694890251303377 1104 36-37 1.0842653834432596 1104 38-39 1.1591797140030877 1104 40-41 1.1089924855315374 1104 42-43 1.1267566058215408 1104 44-45 1.0407311886711597 1104 46-47 1.0396317067487502 1104 48-49 1.1529674226385218 1104 50-51 0.9801485097432945 1104 52-53 1.0445800895101165 1104 54-55 0.666691101592626 1104 56-57 0.9028441859332759 1104 58-59 1.0339981313803044 1104 60-61 1.0743996558137674 1104 62-63 1.0183456429873168 1104 64-65 1.0677026760860073 1104 66-67 1.1047928463478733 1104 68-69 0.7932017175371584 1104 70-71 0.35714974902603913 1104 72-73 0.4543100491822898 1104 74-75 0.9286959983596574 1104 76-77 1.0130485136159706 1104 78-79 0.9787923802943688 1104 80-81 0.9671785893815077 1104 82-83 0.952335146538843 1104 84-85 1.0011328488674565 1104 86-87 1.0438301258570313 1104 88-89 1.0539534037774416 1104 90-91 1.0700312337298428 1104 92-93 1.0159592131127937 1104 94-95 0.9826683707366364 1104 96-97 1.0333767544439816 1104 98-99 1.1425778545111385 1104 100-101 1.0303792327386034 1104 102-103 1.056808287812565 1104 104-105 1.097998844989057 1104 106-107 1.0416618706961174 1104 108-109 1.0002870134926916 1104 110-111 1.0632925526363728 1104 112-113 1.013675914345665 1104 114-115 1.1108322530612256 1104 116-117 1.1566521833800927 1104 118-119 1.0644518234358777 1104 120-121 1.0331729075948992 1104 122-123 1.080236207502164 1104 124-125 1.5546934626072755 1105 1 0.405686249438741 1105 2 0.36081163347037304 1105 3 0.4847042654639786 1105 4 0.46548094557986985 1105 5 0.2275708190111203 1105 6 0.7118040470870568 1105 7 0.7897084526968641 1105 8 0.911612849924488 1105 9 1.079173489603427 1105 10-11 0.9726853144842664 1105 12-13 1.0187888539537155 1105 14-15 1.2031899628435312 1105 16-17 1.1346179328785695 1105 18-19 1.164747939344693 1105 20-21 1.1154257725537917 1105 22-23 1.1868764508287057 1105 24-25 1.036024528037622 1105 26-27 1.0741758440329718 1105 28-29 1.1075854451012646 1105 30-31 1.0386924534209996 1105 32-33 1.1154332155341962 1105 34-35 1.2830663247011316 1105 36-37 1.4162876362099937 1105 38-39 1.373178386197445 1105 40-41 1.1103953553165624 1105 42-43 1.2182820482429193 1105 44-45 1.2304777848527166 1105 46-47 1.1935347042073943 1105 48-49 1.1770965444844599 1105 50-51 0.9758056195344622 1105 52-53 1.1102868956367793 1105 54-55 1.1012519132828764 1105 56-57 1.0578760765182764 1105 58-59 0.7200740364769764 1105 60-61 0.8606050626971751 1105 62-63 1.115476752170018 1105 64-65 0.879807446747428 1105 66-67 1.0292399202175417 1105 68-69 1.086932167824628 1105 70-71 1.1584335097563319 1105 72-73 1.1100502054468535 1105 74-75 0.9833948545431248 1105 76-77 1.1057815398304953 1105 78-79 1.0036423861464883 1105 80-81 1.0108802261672487 1105 82-83 1.0924054773158574 1105 84-85 1.0304561011845088 1105 86-87 1.1945150029217402 1105 88-89 1.129201850680019 1105 90-91 0.8868390633717027 1105 92-93 0.995137136088541 1105 94-95 0.9403031339681434 1105 96-97 1.0007010015223408 1105 98-99 0.9280926315651783 1105 100-101 1.027039102068379 1105 102-103 0.7432499753458401 1105 104-105 0.9083234380281482 1105 106-107 0.9256156911975424 1105 108-109 0.9939901207605892 1105 110-111 0.8677620434103872 1105 112-113 0.8126107785436574 1105 114-115 0.7714647928839682 1105 116-117 0.6294495551118224 1105 118-119 0.8119481288494619 1105 120-121 0.7972254014233471 1105 122-123 0.8383201831613114 1105 124-125 1.1445009248273124 1106 1 0.20849831573286437 1106 2 -0.21372376835187623 1106 3 0.34420528494051084 1106 4 0.15567920296444981 1106 5 0.4190358749130283 1106 6 0.7035495100289424 1106 7 0.6062565271957538 1106 8 0.7040910386749175 1106 9 0.9058942998975681 1106 10-11 0.8865333264030539 1106 12-13 0.9353007021557431 1106 14-15 0.813332744631488 1106 16-17 0.8017756739241833 1106 18-19 0.7892032190418945 1106 20-21 0.8973183762699222 1106 22-23 0.7953997580448373 1106 24-25 0.93930674068109 1106 26-27 0.9671216804157652 1106 28-29 1.1510376235337247 1106 30-31 1.073142111514926 1106 32-33 1.0592898495564071 1106 34-35 0.9878659112807568 1106 36-37 1.0472876676224416 1106 38-39 1.0364310405487984 1106 40-41 0.9899919236908445 1106 42-43 0.8241992956318143 1106 44-45 0.8390795721798767 1106 46-47 0.6785113468129538 1106 48-49 0.8846795488030672 1106 50-51 1.0117340591356765 1106 52-53 0.9388619579109196 1106 54-55 0.864184507485529 1106 56-57 0.3998300854394081 1106 58-59 0.816321249132681 1106 60-61 0.8783351425174146 1106 62-63 0.8093183428960877 1106 64-65 0.9224488680094893 1106 66-67 0.7966785170523103 1106 68-69 0.8637042885071509 1106 70-71 1.0110751941485923 1106 72-73 0.9220480143270287 1106 74-75 0.7923313271624899 1106 76-77 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-3.7653892406199994 2216 106-107 -3.9926095096441294 2216 108-109 -4.130940160521277 2216 110-111 -4.045524532549695 2216 112-113 -4.05611486034509 2216 114-115 -4.222219677991397 2216 116-117 -4.302121442160253 2216 118-119 -4.110925417895405 2216 120-121 -4.239055669507927 2216 122-123 -4.120770134768982 2216 124-125 -4.121084039481879 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71608.0 3 22790.0 4 10847.0 5 8801.0 6 10014.0 7 11688.0 8 13244.0 9 14342.0 10 15633.0 11 17297.0 12 18950.0 13 22259.0 14 26483.0 15 22395.0 16 14235.0 17 12256.0 18 11438.0 19 10422.0 20 10374.0 21 10673.0 22 11747.0 23 14596.0 24 19208.0 25 25096.0 26 30834.0 27 33465.0 28 37783.0 29 44373.0 30 51572.0 31 60843.0 32 73463.0 33 91022.0 34 116902.0 35 162228.0 36 250222.0 37 383726.0 38 151006.0 39 287.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.532734188785966 21.140239776350978 15.059755830991453 22.267270203871607 2 15.289062336739597 22.64100474369423 38.86877520740602 23.201157712160157 3 17.66757813765267 28.390719086871165 30.625216156545925 23.316486618930245 4 12.073917117780436 19.002993247935475 38.34887130916423 30.574218325119855 5 12.10805789808591 40.24461345724045 34.04777751888333 13.59955112579031 6 28.839070863821636 39.48760841785424 18.052663309862172 13.620657408461948 7 26.467020256164307 34.704485278598014 22.16172330994878 16.666771155288902 8 26.018889029953172 35.02401488802892 20.353799670954498 18.60329641106341 9 26.953401122953892 16.16925079111981 20.799933544368276 36.077414541558014 10-11 24.01523423418717 28.021613177926035 27.70517368385307 20.257978904033727 12-13 25.226291741069794 25.877660880549936 28.389726464666303 20.506320913713964 14-15 22.58385896611056 26.616048145324108 27.036319272313698 23.763773616251633 16-17 21.12465463831486 29.83499175729377 27.4622260452799 21.578127559111476 18-19 20.823723667949523 27.44705900484748 31.175491624342342 20.553725702860657 20-21 22.12068611969871 26.63226403043732 30.991603092318964 20.255446757545002 22-23 21.468824871141965 26.588012676307986 30.83403252248289 21.10912993006715 24-25 21.213433626487756 26.82073034007237 31.22018868180816 20.745647351631714 26-27 21.100353060045286 27.20278540239337 30.960304515595137 20.736557021966206 28-29 20.79295363618411 26.609693634992965 31.66778293128651 20.929569797536416 30-31 21.038627631885536 26.682781975234597 31.443816015907032 20.834774376972838 32-33 21.022797919881807 26.848784978177985 30.99645686116141 21.131960240778803 34-35 21.358826657861936 26.64963361791986 31.041804022941065 20.949735701277138 36-37 21.774865969880707 26.317079057656724 30.706036501330637 21.202018471131932 38-39 21.238458154704873 26.73434608661308 30.69323689921541 21.333958859466637 40-41 21.227696040273294 26.434182356192554 31.216583895906318 21.121537707627834 42-43 21.570046214400126 26.577929724437627 30.32604504833025 21.525979012831993 44-45 21.29903423083795 26.828436222978475 30.107224095433832 21.765305450749743 46-47 21.482173027633557 26.93937481518942 29.552348282920317 22.02610387425671 48-49 21.838785615545927 27.102138735148547 29.059877061127764 21.999198588177766 50-51 21.90180816724966 27.228928377288682 28.51797992864086 22.351283526820804 52-53 22.013774461606943 27.401205356816337 28.52746585640832 22.057554325168407 54-55 21.740855598545966 27.2353329620578 28.693338251166857 22.330473188229384 56-57 21.888447110210137 27.669385260034062 28.31416733717176 22.12800029258404 58-59 21.835984570346533 26.986977997305793 28.73006282565782 22.446974606689857 60-61 22.101125215634113 27.439604163162013 28.5957478154475 21.86352280575637 62-63 21.86448930632426 27.350294286362104 28.8546132378187 21.930603169494944 64-65 21.67830998748247 26.846988374790243 29.003858700308655 22.47084293741863 66-67 21.93977186407136 27.452821711468754 28.37501998305228 22.232386441407602 68-69 21.79709548294205 27.20461391698126 28.724553607345822 22.27373699273087 70-71 21.77076487287644 27.22078321026559 28.91962999223665 22.088821924621314 72-73 21.941678743570158 27.356014924858503 28.353025564723673 22.349280766847674 74-75 21.74883300550388 27.638034486091414 28.17901839240794 22.43411411599677 76-77 21.784139151005004 27.474502670153733 28.550191680572084 22.19116649826918 78-79 21.759532568979406 27.44694434315054 28.373374319923183 22.42014876794687 80-81 21.43294493921753 28.10336964068625 28.214020952698394 22.249664467397828 82-83 22.057711054990225 27.74713419520804 27.926354746458166 22.26880000334357 84-85 21.582088289043227 27.459195390889402 27.982646874128193 22.97606944593918 86-87 21.67764123707893 27.316571253034027 28.25104669399338 22.754740815893655 88-89 21.801664178983614 27.256899728697135 28.235298420090515 22.70613767222873 90-91 21.594600553151786 27.59380018619503 28.409187084208842 22.402412176444344 92-93 21.68126636229083 27.467254520576716 28.247890240799634 22.603588876332818 94-95 21.55191779834305 27.05180234070765 28.28362037529478 23.112659485654518 96-97 22.016860770247384 27.13138845733958 28.079313698572523 22.772437073840518 98-99 21.507567827804454 27.2059889559164 28.06369613436176 23.222747081917383 100-101 21.30742433456421 27.354207025397002 28.26644494181029 23.0719236982285 102-103 21.94998542412657 26.866208110036872 28.23291827793631 22.950888187900247 104-105 21.517071534625273 26.93250482466635 28.388603234276605 23.161820406431772 106-107 21.518162350183925 27.24185346928604 28.2652080850657 22.974776095464335 108-109 21.72458181871375 27.238493680131153 27.5628982896597 23.474026211495403 110-111 21.292703359062294 27.38892352910906 27.851799298946954 23.466573812881684 112-113 21.333122967083604 27.101302842765506 28.197027148739735 23.36854704141115 114-115 20.986906790685232 27.396320610702972 27.987897323159128 23.628875275452664 116-117 20.99069442804586 27.486100676968345 28.013000216287153 23.51020467869864 118-119 20.898119346624718 27.392115027150833 27.891769699110085 23.817995927114364 120-121 20.756464191442202 27.56553005912693 27.909398791339544 23.768606958091322 122-123 20.84245888278197 27.615122985935507 27.712952707362437 23.829465423920084 124-125 20.817814125072225 27.502477234282306 27.614090037988614 24.065618602656848 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 693.0 1 555.0 2 821.5 3 1107.5 4 695.0 5 466.0 6 906.0 7 2100.0 8 3588.0 9 4195.0 10 3139.5 11 1838.5 12 1461.5 13 1458.0 14 1597.0 15 1766.5 16 1985.0 17 2296.0 18 2615.5 19 2900.5 20 3348.0 21 3963.0 22 4658.5 23 5462.5 24 6134.5 25 7449.0 26 10448.5 27 13379.0 28 15696.5 29 19184.0 30 24458.0 31 29904.5 32 37400.5 33 46041.5 34 53631.5 35 59183.5 36 65648.5 37 71858.0 38 71824.5 39 70405.5 40 67987.5 41 66910.0 42 67022.0 43 67224.0 44 67494.5 45 66644.0 46 62919.5 47 58117.5 48 60874.5 49 61857.5 50 57611.0 51 53483.5 52 50506.0 53 50335.0 54 49946.5 55 46648.5 56 41931.5 57 37976.0 58 35198.0 59 32384.5 60 29232.5 61 25237.0 62 21254.0 63 17122.0 64 13612.0 65 11567.5 66 8841.0 67 7094.5 68 5176.0 69 3080.5 70 2333.0 71 2067.0 72 1881.5 73 1823.0 74 1470.5 75 1179.5 76 862.0 77 477.5 78 293.0 79 170.0 80 109.5 81 80.0 82 53.5 83 37.0 84 25.0 85 17.5 86 12.0 87 8.5 88 6.5 89 2.5 90 1.5 91 1.5 92 1.0 93 0.5 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09612762404904181 2 1.0448654787939328E-4 3 0.0 4 0.09497827202236847 5 0.0 6 0.0 7 0.001985244409708472 8 0.00527657066790936 9 0.003291326258200888 10-11 6.269192872763597E-4 12-13 0.0 14-15 2.612163696984832E-5 16-17 2.8733800666833146E-4 18-19 0.0017501496769798373 20-21 0.0012799602115225676 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 7.836491090954496E-5 52-53 0.0 54-55 0.0 56-57 0.007418544899436922 58-59 8.097707460652978E-4 60-61 0.0 62-63 0.0 64-65 3.1345964363817984E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 8.881356569748427E-4 76-77 0.0 78-79 0.0 80-81 5.224327393969664E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0013060818484924158 90-91 0.0 92-93 0.002141974231527562 94-95 0.0 96-97 0.007967099275803738 98-99 0.0034219344430501297 100-101 0.001985244409708472 102-103 0.0 104-105 0.0 106-107 0.0013060818484924158 108-109 0.0 110-111 7.836491090954496E-5 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010187438418240843 122-123 0.004283948463055124 124-125 0.08917926861506216 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1914122.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.9305751977124 #Duplication Level Percentage of deduplicated Percentage of total 1 75.46309329294229 31.642109079717034 2 11.654972179281954 9.77399374781256 3 4.602911824431652 5.790082211483129 4 2.4030812941581994 4.030503236436656 5 1.3889638112453817 2.9120025767162843 6 0.8516758132329029 2.142675403850106 7 0.6295458650430259 1.8478054164216853 8 0.43541638514900227 1.4605807583845076 9 0.3120684750823079 1.1776689595146734 >10 1.8507877816333236 14.703910283418184 >50 0.2262540249037636 6.598519218421248 >100 0.17151185437194363 13.616301347063153 >500 0.007698198830912304 2.182377255840788 >1k 0.001766799731684791 1.233004520594205 >5k 2.523999616692559E-4 0.888465984325853 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9161 0.4786006325615609 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7629 0.3985639368859456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4842 0.2529619324160111 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0448654787939326E-4 0.0 10-11 0.0 0.0 0.0 1.567298218190899E-4 0.0 12-13 0.0 0.0 0.0 1.567298218190899E-4 0.0 14-15 0.0 0.0 0.0 1.828514587889382E-4 5.224327393969663E-5 16-17 0.0 0.0 0.0 2.0897309575878653E-4 5.224327393969663E-5 18-19 0.0 2.6121636969848316E-5 0.0 2.612163696984832E-4 5.224327393969663E-5 20-21 0.0 5.224327393969663E-5 0.0 6.007976503065113E-4 5.224327393969663E-5 22-23 0.0 5.224327393969663E-5 0.0 0.0011232303897034776 5.224327393969663E-5 24-25 0.0 5.224327393969663E-5 0.0 0.0020374876836481686 5.224327393969663E-5 26-27 0.0 5.224327393969663E-5 0.0 0.0027688935188039214 5.224327393969663E-5 28-29 0.0 5.224327393969663E-5 0.0 0.004231705189115427 5.224327393969663E-5 30-31 0.0 5.224327393969663E-5 0.0 0.00901196475459767 5.224327393969663E-5 32-33 0.0 5.224327393969663E-5 0.0 0.014471386881295966 5.224327393969663E-5 34-35 0.0 5.224327393969663E-5 0.0 0.02155035050012486 5.224327393969663E-5 36-37 0.0 5.224327393969663E-5 0.0 0.0341671011565616 5.224327393969663E-5 38-39 0.0 5.224327393969663E-5 0.0 0.054777072725771925 5.224327393969663E-5 40-41 0.0 5.224327393969663E-5 0.0 0.08296231901623825 5.224327393969663E-5 42-43 0.0 5.224327393969663E-5 0.0 0.11054676765639808 5.224327393969663E-5 44-45 0.0 5.224327393969663E-5 0.0 0.14048216362384425 5.224327393969663E-5 46-47 0.0 5.224327393969663E-5 0.0 0.17929891616103882 5.224327393969663E-5 48-49 0.0 5.224327393969663E-5 0.0 0.22336611772917297 5.224327393969663E-5 50-51 0.0 5.224327393969663E-5 0.0 0.27720281152403037 5.224327393969663E-5 52-53 0.0 5.224327393969663E-5 0.0 0.32936772055281743 5.224327393969663E-5 54-55 0.0 5.224327393969663E-5 0.0 0.3810363184791774 5.224327393969663E-5 56-57 0.0 5.224327393969663E-5 0.0 0.4418474893449843 5.224327393969663E-5 58-59 0.0 5.224327393969663E-5 0.0 0.512663247170243 5.224327393969663E-5 60-61 0.0 5.224327393969663E-5 0.0 0.5949464036252652 5.224327393969663E-5 62-63 0.0 5.224327393969663E-5 0.0 0.6807298594342471 5.224327393969663E-5 64-65 0.0 5.224327393969663E-5 0.0 0.7675843023589928 5.224327393969663E-5 66-67 0.0 5.224327393969663E-5 0.0 0.8685705508844264 5.224327393969663E-5 68-69 0.0 5.224327393969663E-5 0.0 0.9849163219481308 5.224327393969663E-5 70-71 0.0 5.224327393969663E-5 0.0 1.1219243078549852 5.224327393969663E-5 72-73 0.0 5.224327393969663E-5 0.0 1.2674479474140101 5.224327393969663E-5 74-75 0.0 5.224327393969663E-5 0.0 1.4086615168730101 5.224327393969663E-5 76-77 0.0 5.224327393969663E-5 0.0 1.5632232428236028 5.224327393969663E-5 78-79 0.0 5.224327393969663E-5 0.0 1.7414772935058476 5.224327393969663E-5 80-81 0.0 5.224327393969663E-5 0.0 1.9467149951779459 5.224327393969663E-5 82-83 0.0 5.224327393969663E-5 0.0 2.173633655535018 5.224327393969663E-5 84-85 0.0 5.224327393969663E-5 0.0 2.3975744492775277 5.224327393969663E-5 86-87 0.0 5.224327393969663E-5 0.0 2.628097895536439 5.224327393969663E-5 88-89 0.0 5.224327393969663E-5 0.0 2.881294922685179 5.224327393969663E-5 90-91 0.0 5.224327393969663E-5 0.0 3.1814586531057056 5.224327393969663E-5 92-93 0.0 5.224327393969663E-5 0.0 3.5171739314421964 5.224327393969663E-5 94-95 0.0 5.224327393969663E-5 0.0 3.8512174250126168 5.224327393969663E-5 96-97 0.0 5.224327393969663E-5 0.0 4.193515355865509 5.224327393969663E-5 98-99 0.0 5.224327393969663E-5 0.0 4.557833826683984 5.224327393969663E-5 100-101 0.0 5.224327393969663E-5 0.0 4.975179220551251 5.224327393969663E-5 102-103 0.0 5.224327393969663E-5 0.0 5.434711058124822 5.224327393969663E-5 104-105 0.0 5.224327393969663E-5 0.0 5.904064631199056 5.224327393969663E-5 106-107 0.0 5.224327393969663E-5 0.0 6.366913916667799 5.224327393969663E-5 108-109 0.0 5.224327393969663E-5 0.0 6.8474214287281585 5.224327393969663E-5 110-111 0.0 5.224327393969663E-5 0.0 7.390124558413728 5.224327393969663E-5 112-113 0.0 5.224327393969663E-5 0.0 7.982850622896556 5.224327393969663E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6690 0.0 52.83777 1 GTATCAA 11610 0.0 49.751644 1 ATCAACG 14965 0.0 38.244144 3 TCAACGC 15000 0.0 38.154907 4 CAACGCA 15115 0.0 37.94709 5 AACGCAG 15455 0.0 37.189278 6 TATCAAC 15485 0.0 36.76757 2 GTACATG 11715 0.0 34.804047 1 ACGCAGA 17595 0.0 32.226505 7 CGCAGAG 17605 0.0 32.005424 8 TACATGG 12590 0.0 31.314863 2 ACATGGG 12480 0.0 30.87507 3 GCAGAGT 18290 0.0 29.862576 9 GAGTACT 10465 0.0 28.995409 12-13 CATGGGA 8215 0.0 28.273222 4 TACCGTA 110 7.753895E-4 27.044998 7 AGTACTT 11095 0.0 26.356909 12-13 GTACTTT 11655 0.0 26.162537 14-15 CAGAGTA 18210 0.0 24.765125 10-11 AGAGTAC 17855 0.0 24.441145 10-11 >>END_MODULE