FastQCFastQC Report
Thu 26 May 2016
SRR1364519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146673
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44150.043.5536581
GGTATCA26400.041.492221
TCAACGC49600.037.9455454
ATCAACG50150.037.6139343
CAACGCA52100.036.353385
AACGCAG52600.035.3094756
TATCAAC57650.033.2358552
GTACATG69050.032.848391
TACATGG65500.032.6139562
ACGCAGA60450.030.2318347
ACATGGG64500.029.983623
CGCAGAG61200.029.4729618
GTATAGG8750.024.4932981
CATGGGA35200.024.0270844
GCAGAGT74600.023.4607059
AGAGTAC60700.021.71453510-11
GAGTACT36650.021.42339712-13
GGACCGT2806.6462235E-821.2600566
GTATAGC12050.020.7498551
CAGAGTA68900.020.46890610-11
Produced by FastQC (version 0.11.5)