##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364519_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2146673 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.69451658450076 31.0 26.0 31.0 10.0 34.0 2 26.96727074873537 31.0 27.0 31.0 10.0 34.0 3 27.03442909097007 31.0 27.0 31.0 10.0 34.0 4 29.897106359468815 35.0 28.0 37.0 10.0 37.0 5 30.58799267517689 35.0 30.0 37.0 10.0 37.0 6 30.722089018681466 35.0 31.0 37.0 10.0 37.0 7 30.504618076437353 35.0 30.0 37.0 10.0 37.0 8 30.16478010390963 35.0 30.0 37.0 10.0 37.0 9 30.894856366106996 37.0 29.0 39.0 8.0 39.0 10-11 30.981202307011827 36.0 29.5 39.0 8.0 39.0 12-13 30.84792257600482 37.0 29.5 39.0 8.0 39.0 14-15 31.573943493023855 37.0 29.5 40.0 7.0 41.0 16-17 31.84447771039185 37.0 30.0 40.0 4.5 41.0 18-19 31.77046690390199 37.0 30.0 40.0 2.0 41.0 20-21 31.529483298108282 37.0 29.5 40.0 2.0 41.0 22-23 31.589030793232133 37.0 30.0 40.0 2.0 41.0 24-25 31.605216304486056 37.0 30.0 40.0 2.0 41.0 26-27 31.70198581712259 37.0 30.0 40.0 2.0 41.0 28-29 31.650323314263513 37.0 30.0 40.0 2.0 41.0 30-31 31.299540731168648 37.0 30.0 40.0 2.0 41.0 32-33 31.31885177667954 37.0 30.0 40.0 2.0 41.0 34-35 31.042150341481914 37.0 29.5 40.0 2.0 41.0 36-37 30.82630237581597 37.0 29.0 40.0 2.0 41.0 38-39 30.69164842526086 36.0 28.0 40.0 2.0 41.0 40-41 30.72383497626327 36.5 28.5 40.0 2.0 41.0 42-43 30.612761235642317 36.0 28.0 40.0 2.0 41.0 44-45 30.277901431657266 36.0 27.5 40.0 2.0 41.0 46-47 30.061962161912874 36.0 27.5 40.0 2.0 41.0 48-49 29.842349067603685 35.5 27.0 40.0 2.0 41.0 50-51 29.840405827995227 35.5 27.0 40.0 2.0 41.0 52-53 29.664076689835852 35.0 27.0 40.0 2.0 41.0 54-55 29.55573019272148 35.0 26.5 40.0 2.0 41.0 56-57 29.14668279705386 35.0 26.0 39.0 2.0 41.0 58-59 28.91375887245053 35.0 25.5 39.0 2.0 41.0 60-61 28.546685265990675 34.0 24.5 39.0 2.0 40.5 62-63 28.48690624981075 34.0 25.0 39.0 2.0 40.0 64-65 28.29628662586244 34.0 25.0 38.5 2.0 40.0 66-67 28.048638288178964 34.0 24.0 38.0 2.0 40.0 68-69 27.863232546363605 34.0 24.5 37.5 2.0 40.0 70-71 27.714519631075625 34.0 24.5 37.0 2.0 40.0 72-73 27.692889415388372 34.0 25.0 37.0 2.0 39.5 74-75 27.414192566823175 34.0 24.5 36.5 2.0 39.0 76-77 27.11664538567355 33.5 24.0 36.0 2.0 39.0 78-79 26.793924598669662 33.0 23.5 35.5 2.0 38.5 80-81 26.789039597553984 33.0 24.5 35.0 2.0 37.0 82-83 26.594811832076893 33.0 24.0 35.0 2.0 37.0 84-85 26.383507408906713 33.0 24.0 35.0 2.0 36.5 86-87 26.174569205463527 33.0 23.5 35.0 2.0 36.0 88-89 25.946372596105697 33.0 22.5 35.0 2.0 36.0 90-91 25.763944485256953 33.0 21.0 35.0 2.0 35.5 92-93 25.652041554535785 33.0 20.5 35.0 2.0 35.0 94-95 25.584734377336463 33.0 20.0 35.0 2.0 35.0 96-97 25.451702471685252 33.0 20.0 35.0 2.0 35.0 98-99 25.3606322434763 33.0 19.5 35.0 2.0 35.0 100-101 25.30738659311409 33.0 19.0 35.0 2.0 35.0 102-103 24.13901418613827 30.5 17.0 34.0 2.0 35.0 104-105 24.940608327397793 32.0 18.0 35.0 2.0 35.0 106-107 25.030970483161617 33.0 17.5 35.0 2.0 35.0 108-109 24.992144122556162 33.0 16.5 35.0 2.0 35.0 110-111 24.893240610004412 33.0 13.0 35.0 2.0 35.0 112-113 24.87807900877311 33.0 10.5 35.0 2.0 35.0 114-115 24.831013619680313 33.0 8.5 35.0 2.0 35.0 116-117 24.63254091331097 33.0 3.5 35.0 2.0 35.0 118-119 24.509118994835262 33.0 2.0 35.0 2.0 35.0 120-121 24.38126859563613 33.0 2.0 35.0 2.0 35.0 122-123 24.11605703337211 33.0 2.0 35.0 2.0 35.0 124-125 23.127663598508015 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.1671830441020816 1101 2 1.7956373978751792 1101 3 2.1205906332808375 1101 4 2.528222733932555 1101 5 1.9280266659667902 1101 6 1.8921067430577097 1101 7 1.7872672594627943 1101 8 2.3206553453343126 1101 9 2.7736790983313533 1101 10-11 2.7532388672273598 1101 12-13 2.644833606817599 1101 14-15 3.0249023491206835 1101 16-17 2.610770187683098 1101 18-19 2.586192025853446 1101 20-21 2.94453294119754 1101 22-23 2.7523539135481165 1101 24-25 2.6508958032783525 1101 26-27 2.3739958031843216 1101 28-29 2.3709465264906733 1101 30-31 2.5462088447347178 1101 32-33 2.7090532831895544 1101 34-35 2.9110044073261356 1101 36-37 2.9385921615052766 1101 38-39 2.8016099118204494 1101 40-41 2.6405848716114377 1101 42-43 2.7434765551727587 1101 44-45 2.840719338835605 1101 46-47 2.7903733962748163 1101 48-49 2.7310949149066843 1101 50-51 2.6481679892593455 1101 52-53 2.533325591908561 1101 54-55 2.4681517717878165 1101 56-57 2.7467274623292717 1101 58-59 2.6925271957150976 1101 60-61 2.90511472117878 1101 62-63 2.554611680725383 1101 64-65 2.463761452554131 1101 66-67 2.5442179818116557 1101 68-69 2.3236268149601074 1101 70-71 2.3511440326279747 1101 72-73 2.2562055689086726 1101 74-75 2.170832169354423 1101 76-77 2.4083991400579876 1101 78-79 2.357837433230692 1101 80-81 2.1418609786269442 1101 82-83 2.2180635625626337 1101 84-85 2.1365927774139415 1101 86-87 2.142186402123645 1101 88-89 2.2670097804305804 1101 90-91 2.21350259170206 1101 92-93 2.1540795342659145 1101 94-95 2.236502707121087 1101 96-97 2.1692521739293795 1101 98-99 2.1140313974444496 1101 100-101 2.218906256002377 1101 102-103 2.1850104243796657 1101 104-105 2.1110452338815335 1101 106-107 2.194126596745871 1101 108-109 2.2685447742842406 1101 110-111 2.383990234276933 1101 112-113 2.246093321318316 1101 114-115 2.1748628531714793 1101 116-117 2.3060184746124754 1101 118-119 2.3232540911364232 1101 120-121 2.381168314389651 1101 122-123 2.4471117716302295 1101 124-125 2.3749386949680016 1102 1 0.9525157286130366 1102 2 0.6949324724270411 1102 3 0.7940090660236265 1102 4 1.275241537239875 1102 5 1.169688420479492 1102 6 1.1412185838152915 1102 7 1.0197761870413373 1102 8 1.3877707529427603 1102 9 1.5838293224509918 1102 10-11 1.479638789301788 1102 12-13 1.7305786196226478 1102 14-15 1.9393006876002836 1102 16-17 1.4725827683155863 1102 18-19 1.7063842812887948 1102 20-21 1.6516822363975088 1102 22-23 1.5873898621540299 1102 24-25 1.3541694717083743 1102 26-27 1.415384441936581 1102 28-29 1.1441448947358346 1102 30-31 1.0502547455735929 1102 32-33 1.0824950016324095 1102 34-35 1.5809514731189047 1102 36-37 1.856282855502748 1102 38-39 1.7500578801953708 1102 40-41 1.3127066211241925 1102 42-43 1.3825529698940038 1102 44-45 1.7209383665551954 1102 46-47 1.5643764380477734 1102 48-49 1.740757268075086 1102 50-51 1.6969169995125775 1102 52-53 1.6436742670483788 1102 54-55 1.3691064436851974 1102 56-57 1.480212382643682 1102 58-59 1.7066308049568057 1102 60-61 2.1253340248151105 1102 62-63 1.82711352644111 1102 64-65 1.9313751406996609 1102 66-67 1.8260359470009995 1102 68-69 1.630768668769388 1102 70-71 1.796299561873898 1102 72-73 1.7076818906543387 1102 74-75 1.7959088198590614 1102 76-77 1.6568289648174215 1102 78-79 1.5551246700751058 1102 80-81 1.168035443285774 1102 82-83 1.0932930960208793 1102 84-85 1.141387750378282 1102 86-87 1.320196507364976 1102 88-89 1.0556329810145186 1102 90-91 1.0289479505735777 1102 92-93 0.927635005652931 1102 94-95 1.1992962553517472 1102 96-97 1.3895403313323484 1102 98-99 1.4454581879559285 1102 100-101 1.3040255649062118 1102 102-103 1.155796695097461 1102 104-105 1.1816124016297103 1102 106-107 1.514666758098631 1102 108-109 1.5446177350296502 1102 110-111 1.2890958225941134 1102 112-113 1.2514009141287623 1102 114-115 1.2761139683349434 1102 116-117 1.464169180332231 1102 118-119 1.5685757557002695 1102 120-121 1.5805643911881475 1102 122-123 1.7553741818214235 1102 124-125 1.5909316231385127 1103 1 1.4433250441595362 1103 2 1.211074117941184 1103 3 1.3917389232199966 1103 4 2.012089499684052 1103 5 1.427357254494261 1103 6 1.439957309568804 1103 7 1.4244739329043412 1103 8 1.7831693771138717 1103 9 2.3961568096149257 1103 10-11 1.9346559808778707 1103 12-13 2.1087695362022245 1103 14-15 2.2856368246032766 1103 16-17 2.18155703919248 1103 18-19 1.932869783464664 1103 20-21 2.3794647535325204 1103 22-23 2.2709415504313277 1103 24-25 2.2170076362530864 1103 26-27 1.9207818761472666 1103 28-29 1.8749781938508185 1103 30-31 2.087549906876081 1103 32-33 1.9538654337975458 1103 34-35 1.8078848026532945 1103 36-37 2.11913002855065 1103 38-39 2.089551577176138 1103 40-41 1.996089033341562 1103 42-43 2.056423534044182 1103 44-45 1.9780652657781204 1103 46-47 2.1831405216902624 1103 48-49 2.1703633995556544 1103 50-51 1.9319265988059762 1103 52-53 1.974243554169906 1103 54-55 1.9641503423307398 1103 56-57 2.019336521493628 1103 58-59 2.201998423116944 1103 60-61 2.243694569414746 1103 62-63 2.0972276285615017 1103 64-65 1.9415497632014755 1103 66-67 2.000734245944198 1103 68-69 1.8825803416107192 1103 70-71 1.9726502717020473 1103 72-73 2.0484916252009846 1103 74-75 2.065370161648378 1103 76-77 2.060979161286589 1103 78-79 2.1257522568772735 1103 80-81 1.5927612286387038 1103 82-83 1.7264106163444808 1103 84-85 1.6982752748164636 1103 86-87 1.7066209863592405 1103 88-89 1.8075954520092345 1103 90-91 1.7776054740983405 1103 92-93 1.822317139570405 1103 94-95 1.5764903286784495 1103 96-97 1.6594396958767241 1103 98-99 1.6511753192797904 1103 100-101 1.6005446792739768 1103 102-103 1.5808568425687533 1103 104-105 1.8059219941353035 1103 106-107 1.6157293424747898 1103 108-109 1.861106019366506 1103 110-111 2.0851219832873795 1103 112-113 1.9452110668375546 1103 114-115 1.8220520132970108 1103 116-117 2.026594587830825 1103 118-119 2.0389025003959382 1103 120-121 2.093395265130276 1103 122-123 2.0765866928306522 1103 124-125 2.024499023921372 1104 1 1.273753781420261 1104 2 1.1005654109531129 1104 3 1.1757459377715094 1104 4 1.376950500681673 1104 5 0.9327880918003437 1104 6 0.877016030037165 1104 7 0.8559863735730602 1104 8 1.2951412357060192 1104 9 1.7210827080143254 1104 10-11 1.5345441784629443 1104 12-13 1.077699945099063 1104 14-15 1.3960940904802221 1104 16-17 1.028527573756019 1104 18-19 0.5677300164456973 1104 20-21 -3.8936477456974607 1104 22-23 -0.624503111173361 1104 24-25 0.770440350399717 1104 26-27 0.9149561503115891 1104 28-29 0.6175912342924939 1104 30-31 1.2073591747562666 1104 32-33 1.0187989163607654 1104 34-35 1.3504242415051841 1104 36-37 1.5829054460996659 1104 38-39 1.5291569126513878 1104 40-41 1.558501461450014 1104 42-43 1.4183441115286506 1104 44-45 1.469189474061018 1104 46-47 1.322702696233229 1104 48-49 1.3850801974384908 1104 50-51 1.4143151383984325 1104 52-53 1.6149303930056256 1104 54-55 1.4826272614321923 1104 56-57 1.626945765853307 1104 58-59 1.6054951386459955 1104 60-61 1.6914926818539726 1104 62-63 1.4148864099911798 1104 64-65 1.508914418278028 1104 66-67 1.3804062868602784 1104 68-69 1.4074736393059517 1104 70-71 1.6608047358384717 1104 72-73 1.5405615739118765 1104 74-75 1.5476955398765462 1104 76-77 1.5133542125877 1104 78-79 1.3944182200620787 1104 80-81 1.1755584970597006 1104 82-83 1.1906939248639468 1104 84-85 1.0967357215621156 1104 86-87 1.01701269082006 1104 88-89 1.1654185409879574 1104 90-91 1.211175319206994 1104 92-93 0.8803669934472502 1104 94-95 1.0385397889467924 1104 96-97 1.1910059377075477 1104 98-99 1.0621548364373616 1104 100-101 0.6477218183001803 1104 102-103 1.0425759757020252 1104 104-105 1.2042857987934852 1104 106-107 1.247756594263425 1104 108-109 1.2353092221556814 1104 110-111 1.2915130013333673 1104 112-113 1.2029963950006426 1104 114-115 1.1493047619563797 1104 116-117 1.2529781177150703 1104 118-119 1.2031446480263277 1104 120-121 1.1313247244065963 1104 122-123 1.060831432517027 1104 124-125 1.1231068738509293 1105 1 1.2575253041333774 1105 2 1.0359733630556605 1105 3 1.1286497209471271 1105 4 1.6925111460114728 1105 5 1.2325341388539677 1105 6 1.171771017681067 1105 7 1.1776350586030944 1105 8 1.3207366388362303 1105 9 1.761928949952729 1105 10-11 1.4989676610739977 1105 12-13 1.7008311608184314 1105 14-15 1.9201597485679365 1105 16-17 1.6384208894090868 1105 18-19 1.304563038365206 1105 20-21 1.5653217759307232 1105 22-23 1.4039727693240955 1105 24-25 1.4387840307083124 1105 26-27 1.3849201870383325 1105 28-29 0.9874935909919067 1105 30-31 1.4150502924305037 1105 32-33 1.2757595174898952 1105 34-35 1.2144258086198825 1105 36-37 1.5035152144479724 1105 38-39 1.3323345390753474 1105 40-41 1.5683921652452426 1105 42-43 1.7393448549035746 1105 44-45 1.8515067946967037 1105 46-47 2.0178528780587186 1105 48-49 1.7214067198502363 1105 50-51 1.8542297507384546 1105 52-53 1.7881827587460855 1105 54-55 1.7206320029587232 1105 56-57 1.9827348482875564 1105 58-59 1.68206275562709 1105 60-61 2.1738335092101693 1105 62-63 1.9921178406865359 1105 64-65 1.799944494825553 1105 66-67 1.9435292651818585 1105 68-69 1.5408646344237837 1105 70-71 1.5947641114035385 1105 72-73 1.3786040869209408 1105 74-75 1.8715916281171552 1105 76-77 1.8066566054512982 1105 78-79 1.6572868436129973 1105 80-81 1.6384321434141498 1105 82-83 1.7314453362717366 1105 84-85 1.652267435543589 1105 86-87 1.6546654342753442 1105 88-89 1.712440018495844 1105 90-91 1.7221451221121846 1105 92-93 1.694416578563679 1105 94-95 1.4527263961434649 1105 96-97 1.6466314776589286 1105 98-99 1.4317845553612756 1105 100-101 1.5112878078929306 1105 102-103 1.459358367761979 1105 104-105 1.6015915669810568 1105 106-107 1.7398531124560215 1105 108-109 1.793730052726474 1105 110-111 1.6346633037061409 1105 112-113 1.7409097215029625 1105 114-115 1.660562271047656 1105 116-117 1.7754312549581535 1105 118-119 1.757958502927579 1105 120-121 1.7447106967209045 1105 122-123 1.7909166192648627 1105 124-125 1.53147185084649 1106 1 -0.22252190900283964 1106 2 0.051515567467266266 1106 3 0.15019691260422974 1106 4 -0.18808380496501087 1106 5 -0.6328823206926799 1106 6 -0.18934025026166168 1106 7 -0.01686440646470544 1106 8 0.3911954122356782 1106 9 0.5083875351542346 1106 10-11 -2.053379392298531 1106 12-13 -0.18328276439743973 1106 14-15 0.02325769127802957 1106 16-17 -0.11075376032794537 1106 18-19 -2.893435640157925 1106 20-21 -5.317521035828371 1106 22-23 -6.124515735259138 1106 24-25 -6.0410437681735445 1106 26-27 -4.976058360441762 1106 28-29 -1.6090562497558132 1106 30-31 -3.2489716612687083 1106 32-33 -4.014802151089434 1106 34-35 -3.223262056908599 1106 36-37 -5.632674922752422 1106 38-39 -6.163622355413111 1106 40-41 -2.2656417144758123 1106 42-43 -0.9930063306411867 1106 44-45 -0.19962396809819793 1106 46-47 0.15887094710690874 1106 48-49 0.3101573514384448 1106 50-51 0.17783042743279864 1106 52-53 -0.03251586474960888 1106 54-55 0.07624727726745206 1106 56-57 0.13518692429497747 1106 58-59 0.3774163120568126 1106 60-61 0.522461198446269 1106 62-63 0.2520464891184595 1106 64-65 0.0054246143721563556 1106 66-67 -0.2758362622735575 1106 68-69 -0.1699243854535979 1106 70-71 0.006782718452370773 1106 72-73 -0.03706801498415757 1106 74-75 -0.10599792444223155 1106 76-77 -0.06973613092634068 1106 78-79 -0.16277534353348955 1106 80-81 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12476.0 18 11568.0 19 11100.0 20 11423.0 21 12483.0 22 15138.0 23 20312.0 24 27172.0 25 33873.0 26 41622.0 27 48269.0 28 55896.0 29 63565.0 30 71976.0 31 82602.0 32 98864.0 33 122031.0 34 152364.0 35 195856.0 36 261624.0 37 291244.0 38 90684.0 39 339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.415465933559695 18.954828313272674 13.668540777054847 23.961164976112787 2 14.991640167457604 19.979626985576726 41.09864229609278 23.93009055087289 3 18.96907994810308 24.868579992841806 28.874327054998805 27.28801300405631 4 11.181434697481448 16.361994130414416 38.782002733920045 33.674568438184096 5 13.494138636897505 38.11820229159908 34.65493648325671 13.732722588246698 6 31.707572725981038 37.22121416109774 17.59208230747909 13.479130805442132 7 28.420047376916678 32.211644845489296 21.189966528697507 18.17834124889652 8 25.19253631776272 36.36458077115151 20.099897140046064 18.342985771039704 9 25.489467127692382 16.78136405098862 20.513501400873416 37.21566742044558 10-11 24.278991528486923 26.98966130960163 28.019778283180326 20.71156887873112 12-13 25.082615261304614 24.163953587774596 28.299480118413463 22.453951032507334 14-15 22.23672943410537 25.779429399909244 25.616578505265924 26.367262660719458 16-17 22.277725023972177 28.93707501218411 26.142999984158326 22.642199979685383 18-19 22.157738948037384 27.997715130226865 27.918834940057113 21.92571098167864 20-21 22.84556514478887 27.26786145326444 28.011180571832377 21.87539283011431 22-23 22.43013165897955 27.334572496768338 27.73308801655655 22.502207827695557 24-25 22.270821093670286 27.34660256461028 27.933430831324902 22.44914551039453 26-27 22.181927195020425 27.52802314806835 27.699206486378706 22.59084317053252 28-29 22.255181681156017 27.645206215571633 27.693585576847603 22.406026526424753 30-31 22.431602531508172 27.364740612649946 27.669451425038975 22.534205430802903 32-33 22.17483606236618 27.440942387498442 27.6301531575348 22.754068392600583 34-35 22.561591961915546 27.414960518943804 27.75666763345459 22.266779885686063 36-37 22.632075566186213 27.14225586578724 27.674673900359238 22.55099466766731 38-39 22.34864552287045 27.42811819747798 27.722342379486538 22.500893900165035 40-41 22.566959324164817 27.277889175369424 27.818577825288088 22.33657367517767 42-43 22.447477300750123 27.33805709749176 27.472935263372335 22.74153033838579 44-45 22.36597735862431 27.456707713321787 27.603955233955674 22.57335969409823 46-47 22.602549395261082 27.534516994997876 27.28745043004303 22.575483179698008 48-49 22.54847768029248 27.531618663601183 27.257588304559448 22.662315351546894 50-51 22.634182748594544 27.59260958873157 27.128985113608145 22.64422254906574 52-53 22.709743575886318 27.61760313488781 27.078264341466472 22.594388947759402 54-55 22.575570058447166 27.554306061328177 27.169744163494595 22.700379716730062 56-57 22.380323906707712 27.75282034749812 27.08851996511602 22.778335780678148 58-59 22.51720515948623 27.442925320883827 27.272591737749647 22.7672777818803 60-61 22.568143334678386 27.501889440474002 27.28880901414541 22.6411582107022 62-63 22.457801621968127 27.60099099569165 27.37257561979159 22.568631762548634 64-65 22.43194830726801 27.518902600941985 27.2132464955067 22.835902596283304 66-67 22.266956377521073 27.645203279000125 27.123472169682106 22.964368173796696 68-69 22.5219775356276 27.459993198261362 27.31794867016692 22.700080595944115 70-71 22.373615039505744 27.21655987308222 27.461763129508178 22.94806195790386 72-73 22.420031360433494 27.48759231809132 27.173428973649223 22.918947347825966 74-75 22.32126323841219 27.660510616234706 27.156001595981444 22.862224549371657 76-77 22.549509003828785 27.35772385183625 27.30927296414844 22.783494180186523 78-79 22.375069511632876 27.367140467896284 27.373430586329896 22.884359434140947 80-81 22.5539752088234 27.498561103656204 27.096997801883482 22.850465885636915 82-83 22.618531295057032 27.506271589524754 27.214109853558092 22.661087261860118 84-85 22.526913252887447 27.59278661001324 26.996197275809074 22.88410286129024 86-87 22.53992130804184 27.54137011214955 27.149750442327814 22.768958137480798 88-89 22.562313539218955 27.379919722868735 27.132664108780357 22.925102629131953 90-91 22.43159168490306 27.542168871144877 27.169342825963653 22.856896617988404 92-93 22.56448014206583 27.523763626424074 27.174701698328597 22.737054533181496 94-95 22.45836656430286 27.34995751405018 27.19729692461353 22.994378997033436 96-97 22.533365600897593 27.21182856233793 27.23209284114273 23.022712995621752 98-99 22.269420585163424 27.27560707234743 27.21276391563644 23.24220842685271 100-101 22.371892252485427 27.16137204179053 27.353931383003253 23.11280432272079 102-103 22.294399014654406 26.971902371034417 27.54209100844196 23.191607605869216 104-105 22.274103138360626 27.157050800516636 27.245912980283777 23.32293308083896 106-107 22.215886778161163 27.15445859205004 27.35393191167832 23.275722718110472 108-109 22.242755598806603 27.05681272667687 27.245176823972155 23.455254850544375 110-111 22.147005380813503 27.27311477616901 27.291865284237904 23.288014558779583 112-113 22.286011103722085 27.27616403321101 27.274906216989603 23.1629186460773 114-115 22.282511094821857 27.389075656284945 27.17534325642368 23.15306999246952 116-117 21.980803597217484 27.344298846333743 27.457154463818185 23.217743092630585 118-119 22.156590904538227 27.233557270215464 27.198035765613042 23.41181605963327 120-121 21.997299812999472 27.42355293549833 27.266251668982044 23.312895582520156 122-123 21.95792164884674 27.407460377430564 27.313286750425213 23.321331223297477 124-125 22.150089233403726 27.364452152578167 27.05203036872109 23.433428245297012 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 671.0 1 668.5 2 863.5 3 933.0 4 692.0 5 518.0 6 420.5 7 453.5 8 576.0 9 633.0 10 592.5 11 541.0 12 561.5 13 628.0 14 708.0 15 816.5 16 953.5 17 1121.0 18 1330.0 19 1602.5 20 1977.0 21 2532.5 22 3410.0 23 4626.5 24 6277.5 25 8450.5 26 11333.0 27 14519.5 28 17633.5 29 21344.0 30 25878.5 31 30942.0 32 37085.0 33 42452.5 34 46457.0 35 50411.0 36 55722.0 37 61153.5 38 63884.5 39 65519.5 40 66963.5 41 68662.5 42 70114.0 43 72007.5 44 74358.0 45 76832.5 46 78388.0 47 78387.5 48 78710.0 49 78473.5 50 76495.5 51 74537.0 52 72975.5 53 71308.5 54 69535.5 55 65514.5 56 60148.5 57 54468.5 58 49264.5 59 43323.0 60 36407.5 61 30632.5 62 25361.5 63 19964.5 64 14883.0 65 11232.5 66 8789.0 67 7035.5 68 5794.0 69 4730.0 70 3705.5 71 2783.5 72 2158.5 73 1739.5 74 1275.0 75 877.5 76 629.0 77 459.0 78 320.0 79 216.5 80 161.0 81 127.0 82 91.5 83 63.0 84 35.0 85 27.5 86 31.0 87 25.5 88 19.0 89 14.5 90 11.5 91 10.5 92 9.5 93 6.5 94 3.0 95 3.0 96 2.0 97 1.0 98 1.0 99 2.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09367984783895823 2 0.032468848306192885 3 0.04104025158931985 4 0.0482607271810844 5 0.051102333704294976 6 0.004099366787582459 7 0.0027018553827248028 8 0.003027941377191589 9 0.007220475591764558 10-11 0.05536474348911082 12-13 0.005892839757149785 14-15 0.010760837817403954 16-17 0.02026391537043602 18-19 0.017701811128196982 20-21 0.12929309680608084 22-23 0.01548908473717236 24-25 0.014161448902557588 26-27 0.006708054743316751 28-29 0.0044953283522921285 30-31 0.0022127263910246228 32-33 0.005822964186906902 34-35 0.01819094011989716 36-37 0.0023990612450056435 38-39 0.008408360285893566 40-41 0.032888101727650186 42-43 0.013579152483866895 44-45 0.10828384201972076 46-47 0.00398290750384432 48-49 0.009107115988322395 50-51 0.010062082114975127 52-53 0.011576052803570921 54-55 0.009526369409779691 56-57 0.006009299040887923 58-59 0.013812071051343171 60-61 0.02492228671996154 62-63 0.006754638456812006 64-65 0.006661471029821496 66-67 0.007686312726717111 68-69 0.0075931452997266 70-71 0.0040294912173395765 72-73 8.850905564098492E-4 74-75 0.0014673869751005394 76-77 0.007895939437445758 78-79 0.020939379216117222 80-81 0.002911482093453451 82-83 0.004099366787582459 84-85 0.0025155205287437817 86-87 0.0034937785121441412 88-89 0.005077624770982819 90-91 0.004448744638796873 92-93 0.002911482093453451 94-95 0.003773280793115673 96-97 0.0017934729695673257 98-99 0.0027484390962200576 100-101 0.0028881902367058235 102-103 6.987557024288282E-4 104-105 0.004215826071320598 106-107 9.316742699051043E-4 108-109 0.0015139706885957944 110-111 0.0030046495204439617 112-113 0.004448744638796873 114-115 0.002958065806948706 116-117 0.005822964186906902 118-119 0.004006199360591949 120-121 0.007220475591764558 122-123 0.004937873630497053 124-125 0.05478244707042013 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2146673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.006536543217 #Duplication Level Percentage of deduplicated Percentage of total 1 82.37423882867623 56.01986683122055 2 10.529305852302018 14.321232464385716 3 3.300666882163894 6.734007688165949 4 1.37106422819658 3.7296531815179335 5 0.7162065843817009 2.4353364626623386 6 0.4203069157626339 1.7150170575707033 7 0.2540586687097115 1.209435509640964 8 0.188171438782226 1.0237510262346539 9 0.1352255258906814 0.8276597701254326 >10 0.6553576686367258 7.911789885935742 >50 0.03894028796480198 1.8000930070526313 >100 0.015610359016547342 1.8384927074877777 >500 7.056329302077411E-4 0.31359930356064997 >1k 1.411265860415362E-4 0.12006510443897339 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.316742699051044E-5 2 0.0 0.0 0.0 0.0 9.316742699051044E-5 3 0.0 0.0 0.0 0.0 9.316742699051044E-5 4 0.0 0.0 0.0 0.0 9.316742699051044E-5 5 4.658371349525522E-5 0.0 0.0 0.0 9.316742699051044E-5 6 4.658371349525522E-5 0.0 0.0 0.0 9.316742699051044E-5 7 4.658371349525522E-5 0.0 0.0 0.0 9.316742699051044E-5 8 4.658371349525522E-5 0.0 0.0 0.0 9.316742699051044E-5 9 4.658371349525522E-5 0.0 0.0 0.0 1.8633485398102088E-4 10-11 4.658371349525522E-5 0.0 0.0 0.0 2.3291856747627607E-4 12-13 4.658371349525522E-5 0.0 0.0 0.0 3.9596156470966934E-4 14-15 9.316742699051044E-5 0.0 0.0 2.329185674762761E-5 4.6583713495255215E-4 16-17 9.316742699051044E-5 0.0 0.0 9.316742699051044E-5 4.6583713495255215E-4 18-19 9.316742699051044E-5 0.0 0.0 9.316742699051044E-5 4.6583713495255215E-4 20-21 9.316742699051044E-5 0.0 0.0 1.3975114048576565E-4 5.124208484478073E-4 22-23 9.316742699051044E-5 4.658371349525522E-5 0.0 2.795022809715313E-4 5.124208484478073E-4 24-25 9.316742699051044E-5 4.658371349525522E-5 0.0 6.987557024288282E-4 5.124208484478073E-4 26-27 9.316742699051044E-5 4.658371349525522E-5 0.0 0.001374219548110029 5.124208484478073E-4 28-29 9.316742699051044E-5 4.658371349525522E-5 0.0 0.0020496833937912297 5.124208484478073E-4 30-31 9.316742699051044E-5 4.658371349525522E-5 0.0 0.004472036495544501 5.124208484478073E-4 32-33 9.316742699051044E-5 4.658371349525522E-5 0.0 0.008944072991089002 5.590045619430626E-4 34-35 1.1645928373813804E-4 4.658371349525522E-5 0.0 0.014906788318481669 6.52171988933573E-4 36-37 1.6304299723339326E-4 4.658371349525522E-5 0.0 0.02580737727637139 6.52171988933573E-4 38-39 2.3291856747627607E-4 4.658371349525522E-5 0.0 0.0455588717983596 6.52171988933573E-4 40-41 2.3291856747627607E-4 4.658371349525522E-5 0.0 0.0695261923916684 6.52171988933573E-4 42-43 2.562104242239037E-4 4.658371349525522E-5 0.0 0.09323730256075331 6.52171988933573E-4 44-45 3.7266970796204175E-4 4.658371349525522E-5 0.0 0.12156020036586848 6.987557024288283E-4 46-47 4.192534214572969E-4 4.658371349525522E-5 0.0 0.15340016853987543 7.68631272671711E-4 48-49 4.192534214572969E-4 4.658371349525522E-5 0.0 0.19038763705510808 8.385068429145938E-4 50-51 4.192534214572969E-4 4.658371349525522E-5 0.0 0.23275552447904269 8.385068429145938E-4 52-53 4.192534214572969E-4 4.658371349525522E-5 0.0 0.27400540277909113 8.385068429145938E-4 54-55 4.192534214572969E-4 4.658371349525522E-5 0.0 0.32163724982798964 8.385068429145938E-4 56-57 4.6583713495255215E-4 4.658371349525522E-5 0.0 0.3763731131849145 8.617986996622215E-4 58-59 4.6583713495255215E-4 4.658371349525522E-5 0.0 0.4389350404090423 8.850905564098491E-4 60-61 4.6583713495255215E-4 4.658371349525522E-5 0.0 0.511885135742612 8.850905564098491E-4 62-63 4.6583713495255215E-4 4.658371349525522E-5 0.0 0.5862793261945345 8.850905564098491E-4 64-65 4.6583713495255215E-4 4.658371349525522E-5 0.0 0.6609996026409239 8.850905564098491E-4 66-67 4.6583713495255215E-4 4.658371349525522E-5 0.0 0.7455024589213168 8.850905564098491E-4 68-69 4.6583713495255215E-4 4.658371349525522E-5 0.0 0.8469152032004874 8.850905564098491E-4 70-71 4.6583713495255215E-4 4.658371349525522E-5 0.0 0.9639334915005686 9.316742699051043E-4 72-73 4.6583713495255215E-4 4.658371349525522E-5 0.0 1.0886380925273667 0.0010481335536432425 74-75 4.6583713495255215E-4 4.658371349525522E-5 0.0 1.2171392662040281 0.0011180091238861252 76-77 4.6583713495255215E-4 4.658371349525522E-5 0.0 1.360011515493976 0.0011180091238861252 78-79 4.6583713495255215E-4 4.658371349525522E-5 0.0 1.528132137498352 0.0011180091238861252 80-81 5.124208484478073E-4 4.658371349525522E-5 0.0 1.7156315843167542 0.0011180091238861252 82-83 5.124208484478073E-4 4.658371349525522E-5 0.0 1.9138452852390653 0.0012111765508766355 84-85 5.590045619430625E-4 4.658371349525522E-5 0.0 2.116950276078378 0.0012111765508766355 86-87 6.055882754383178E-4 4.658371349525522E-5 0.0 2.328254000492856 0.0012344684076242632 88-89 6.055882754383178E-4 6.987557024288283E-5 0.0 2.561335610966365 0.0012577602643718908 90-91 6.52171988933573E-4 9.316742699051044E-5 0.0 2.823415583090671 0.0012577602643718908 92-93 6.52171988933573E-4 9.316742699051044E-5 0.0 3.1096725025190146 0.0013509276913624012 94-95 6.52171988933573E-4 9.316742699051044E-5 0.0 3.3946250779694904 0.0013975114048576564 96-97 6.52171988933573E-4 9.316742699051044E-5 0.0 3.688242224130084 0.001420803261605284 98-99 7.453394159240835E-4 9.316742699051044E-5 0.0 4.009530096106859 0.001490678831848167 100-101 7.453394159240835E-4 9.316742699051044E-5 0.0 4.376633050306218 0.001490678831848167 102-103 7.453394159240835E-4 9.316742699051044E-5 0.0 4.769030960933501 0.001537262545343422 104-105 9.316742699051043E-4 9.316742699051044E-5 0.0 5.158866767318544 0.0015838462588386774 106-107 9.549661266527319E-4 9.316742699051044E-5 0.0 5.555340752876661 0.0015838462588386774 108-109 9.782579834003596E-4 9.316742699051044E-5 0.0 5.971123687678562 0.0015838462588386774 110-111 9.782579834003596E-4 9.316742699051044E-5 0.0 6.427341285794343 0.0015838462588386774 112-113 9.782579834003596E-4 9.316742699051044E-5 0.0 6.936897235862192 0.0015838462588386774 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4415 0.0 43.553658 1 GGTATCA 2640 0.0 41.49222 1 TCAACGC 4960 0.0 37.945545 4 ATCAACG 5015 0.0 37.613934 3 CAACGCA 5210 0.0 36.35338 5 AACGCAG 5260 0.0 35.309475 6 TATCAAC 5765 0.0 33.235855 2 GTACATG 6905 0.0 32.84839 1 TACATGG 6550 0.0 32.613956 2 ACGCAGA 6045 0.0 30.231834 7 ACATGGG 6450 0.0 29.98362 3 CGCAGAG 6120 0.0 29.472961 8 GTATAGG 875 0.0 24.493298 1 CATGGGA 3520 0.0 24.027084 4 GCAGAGT 7460 0.0 23.460705 9 AGAGTAC 6070 0.0 21.714535 10-11 GAGTACT 3665 0.0 21.423397 12-13 GGACCGT 280 6.6462235E-8 21.260056 6 GTATAGC 1205 0.0 20.749855 1 CAGAGTA 6890 0.0 20.468906 10-11 >>END_MODULE