FastQCFastQC Report
Thu 26 May 2016
SRR1364519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146673
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38750.18051188979411398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34340.1599684721427064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23210.10812079902248735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37800.050.35641
GTATCAA62200.045.951631
TCAACGC75400.035.5738074
ATCAACG76700.035.359433
CAACGCA77100.034.565425
TATCAAC82600.034.421322
AACGCAG79500.033.1478046
CGCAGAG93900.028.9439288
ACGCAGA91300.028.863647
GTACATG67450.026.2742521
TACATGG65550.025.642812
ACATGGG62700.025.0972183
GCAGAGT106000.024.9666929
AGAGTAC92900.024.32735810-11
GAGTACT66050.023.50145712-13
GTACTTT70700.022.37635214-15
AGTACTT71750.022.17323112-13
CAGAGTA101300.021.9578210-11
GTGGTAT27550.019.9260881
TGGTATC25650.019.5200582
Produced by FastQC (version 0.11.5)