##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364519_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2146673 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.96739326390186 31.0 28.0 31.0 25.0 34.0 2 29.20386756622923 31.0 28.0 34.0 25.0 34.0 3 29.27392714214042 31.0 28.0 34.0 25.0 34.0 4 32.69670834822071 35.0 32.0 37.0 26.0 37.0 5 31.849551375547183 35.0 32.0 37.0 22.0 37.0 6 31.88557968540155 35.0 32.0 37.0 22.0 37.0 7 31.351522565383735 35.0 32.0 37.0 17.0 37.0 8 31.464686517229218 35.0 32.0 37.0 17.0 37.0 9 32.12258364455136 37.0 31.0 39.0 11.0 39.0 10-11 32.273932033430334 37.0 31.5 39.0 16.5 39.0 12-13 32.04890707620583 37.0 30.5 39.0 13.5 39.0 14-15 32.99032991983408 38.0 31.5 40.0 13.5 41.0 16-17 33.04039134046033 38.0 32.0 40.0 14.5 41.0 18-19 32.880134748049656 38.0 31.0 40.0 11.0 41.0 20-21 32.7661162179801 38.0 31.0 40.0 10.5 41.0 22-23 32.735989365869884 38.0 31.0 40.0 10.0 41.0 24-25 32.541150654990304 38.0 31.0 40.0 10.0 41.0 26-27 32.61285137512793 38.0 31.0 40.0 10.0 41.0 28-29 32.49669791346889 38.0 31.0 40.0 9.5 41.0 30-31 31.99964433334746 37.0 30.0 40.0 8.0 41.0 32-33 31.943887122072155 37.0 30.0 40.0 8.0 41.0 34-35 31.750299183899923 37.0 30.0 40.0 6.5 41.0 36-37 31.730403046947533 37.0 30.0 40.0 2.0 41.0 38-39 31.49644589557888 37.0 30.0 40.0 2.0 41.0 40-41 31.23091616655168 37.0 29.5 40.0 2.0 41.0 42-43 31.11631673757484 36.5 29.5 40.0 2.0 41.0 44-45 31.04369924995563 36.0 29.0 40.0 2.0 41.0 46-47 30.914671447397904 36.0 29.0 40.0 2.0 41.0 48-49 30.660296887322847 36.0 28.0 40.0 2.0 41.0 50-51 30.339307616949576 36.0 28.0 40.0 2.0 41.0 52-53 30.246166276838622 36.0 28.0 40.0 2.0 41.0 54-55 29.78259683705902 35.0 26.5 40.0 2.0 41.0 56-57 29.79937489314861 35.0 27.0 40.0 2.0 41.0 58-59 29.554352712313424 35.0 26.5 39.0 2.0 41.0 60-61 29.285010106336642 35.0 26.0 39.0 2.0 41.0 62-63 29.01880910599798 35.0 26.0 39.0 2.0 41.0 64-65 28.577880748488475 34.0 25.5 38.5 2.0 40.0 66-67 28.19473156833854 34.0 24.0 38.0 2.0 40.0 68-69 27.999183853339563 34.0 23.5 38.0 2.0 40.0 70-71 28.059263567390097 34.0 25.5 37.0 2.0 40.0 72-73 27.822765274450276 34.0 25.0 37.0 2.0 39.5 74-75 27.47880254701112 34.0 24.5 36.0 2.0 39.0 76-77 27.374672574723768 34.0 25.0 36.0 2.0 39.0 78-79 26.96984380015028 33.5 24.0 35.5 2.0 38.5 80-81 26.742265822507665 33.0 23.5 35.0 2.0 37.0 82-83 26.606486409434506 33.0 24.0 35.0 2.0 37.0 84-85 26.197944679976878 33.0 21.5 35.0 2.0 36.0 86-87 26.061903233515302 33.0 21.5 35.0 2.0 36.0 88-89 25.982978311088832 33.0 21.5 35.0 2.0 36.0 90-91 25.812913983638868 33.0 20.0 35.0 2.0 35.5 92-93 25.82834879834982 33.0 22.0 35.0 2.0 35.0 94-95 25.832089936380623 33.0 23.0 35.0 2.0 35.0 96-97 25.70936630777021 33.0 20.0 35.0 2.0 35.0 98-99 25.592841340996046 33.0 20.0 35.0 2.0 35.0 100-101 25.48172148249873 33.0 20.0 35.0 2.0 35.0 102-103 24.347815666382353 30.5 18.5 34.0 2.0 35.0 104-105 25.29673313075629 32.5 19.5 35.0 2.0 35.0 106-107 25.39467189460155 33.0 19.0 35.0 2.0 35.0 108-109 25.467897532600446 33.0 19.5 35.0 2.0 35.0 110-111 25.39127035184213 33.0 19.0 35.0 2.0 35.0 112-113 25.34088005019861 33.0 19.0 35.0 2.0 35.0 114-115 25.292174914390777 33.0 18.5 35.0 2.0 35.0 116-117 25.209996119576665 33.0 18.0 35.0 2.0 35.0 118-119 25.091260289760015 33.0 16.0 35.0 2.0 35.0 120-121 24.932161069711128 33.0 4.5 35.0 2.0 35.0 122-123 24.646678604519643 33.0 2.0 35.0 2.0 35.0 124-125 23.87647210357609 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9742264388021837 1101 2 0.9722290474143449 1101 3 0.9775336611178886 1101 4 0.9614541544453132 1101 5 1.204991835849782 1101 6 1.2115271000694108 1101 7 1.6989465732364195 1101 8 1.5316620109315942 1101 9 2.233976611882504 1101 10-11 1.9430724248771654 1101 12-13 2.0582899035145523 1101 14-15 2.0689929612972193 1101 16-17 1.9404717092951742 1101 18-19 2.01797390843808 1101 20-21 2.2226985065996843 1101 22-23 2.2878034459729832 1101 24-25 2.4580704954338657 1101 26-27 2.191263835106561 1101 28-29 2.246096647108047 1101 30-31 2.691546039953 1101 32-33 2.454384674448878 1101 34-35 2.593901535409273 1101 36-37 2.53065181194464 1101 38-39 2.641612280730012 1101 40-41 2.816352640934671 1101 42-43 2.6034128417802265 1101 44-45 2.584615567491518 1101 46-47 2.3607155080278623 1101 48-49 2.4503061506101425 1101 50-51 2.665996828583822 1101 52-53 2.4972995184676883 1101 54-55 2.950511503507819 1101 56-57 2.666662235821377 1101 58-59 2.829596556826097 1101 60-61 2.913233552075887 1101 62-63 2.837172345697116 1101 64-65 3.091413998562505 1101 66-67 3.1636787716480406 1101 68-69 3.1132339119425616 1101 70-71 2.6775736166987087 1101 72-73 2.9093464680033527 1101 74-75 2.9967206591424826 1101 76-77 2.8191699592490416 1101 78-79 2.8758883138279714 1101 80-81 2.920321385406808 1101 82-83 2.7932925358724248 1101 84-85 2.9886485027304985 1101 86-87 2.9302863533365944 1101 88-89 2.791021146310733 1101 90-91 2.7846959591965934 1101 92-93 2.698084737831639 1101 94-95 2.6471071897083718 1101 96-97 2.4677947335195363 1101 98-99 2.6109586466338577 1101 100-101 2.702227115563435 1101 102-103 2.350718994130929 1101 104-105 2.482256182210115 1101 106-107 2.5980082797108253 1101 108-109 2.535236612736881 1101 110-111 2.5910064767530123 1101 112-113 2.511671258525162 1101 114-115 2.5299080927833195 1101 116-117 2.6521382315542503 1101 118-119 2.4771826699203245 1101 120-121 2.4746179161005557 1101 122-123 2.5485781586974916 1101 124-125 2.3749529466956716 1102 1 0.5701106808630954 1102 2 -1.7544969998777837 1102 3 -0.02550961683979125 1102 4 0.5392797299198264 1102 5 1.062050329361135 1102 6 0.5708305524566342 1102 7 1.3230667960099112 1102 8 0.9883178332345288 1102 9 1.7890688224186775 1102 10-11 1.5590688647151936 1102 12-13 1.74421480566863 1102 14-15 1.7625640596046779 1102 16-17 1.6280359749009818 1102 18-19 1.8218171777002041 1102 20-21 2.036931295039274 1102 22-23 1.8076324946538875 1102 24-25 2.058541184297617 1102 26-27 1.7491249536729967 1102 28-29 1.7493438108094423 1102 30-31 2.044104582376871 1102 32-33 1.8980722848678262 1102 34-35 2.067924685659321 1102 36-37 1.553277611162546 1102 38-39 1.462647294563471 1102 40-41 1.581865055933509 1102 42-43 1.5650559426495292 1102 44-45 1.433226159516856 1102 46-47 1.1569649217048052 1102 48-49 1.2129775847835127 1102 50-51 1.3449512797478178 1102 52-53 1.3212239239716403 1102 54-55 1.559354760228274 1102 56-57 1.1506384551434081 1102 58-59 1.326897087840397 1102 60-61 1.321693284536991 1102 62-63 1.5174125688385018 1102 64-65 1.6606884546093887 1102 66-67 1.8222186871840762 1102 68-69 1.7474884727207503 1102 70-71 1.1259133922697089 1102 72-73 1.5307779658215246 1102 74-75 1.6090050573889876 1102 76-77 1.5169414299604824 1102 78-79 1.3889420180937293 1102 80-81 1.3738974752988078 1102 82-83 1.067381028221586 1102 84-85 1.124241552304376 1102 86-87 1.098852581295656 1102 88-89 1.2632237059819076 1102 90-91 1.047107195529307 1102 92-93 1.1167776366546462 1102 94-95 1.1376805363191522 1102 96-97 1.1800430953684469 1102 98-99 0.8651938430740316 1102 100-101 0.8361495729280257 1102 102-103 0.619670543988665 1102 104-105 1.0773008012903418 1102 106-107 1.2853884754110396 1102 108-109 1.1766705269167446 1102 110-111 1.1220618248136098 1102 112-113 1.0690628169781178 1102 114-115 1.3493929980477262 1102 116-117 0.956676267512055 1102 118-119 0.40216517429109544 1102 120-121 0.6929684273408157 1102 122-123 1.083149647468936 1102 124-125 1.2351331036003188 1103 1 0.7218627299833216 1103 2 0.738171922127016 1103 3 0.5823843459546723 1103 4 0.8035941576871366 1103 5 1.0604540225960477 1103 6 0.9340715018439312 1103 7 1.381218066795462 1103 8 1.1099278496844711 1103 9 1.861726381139455 1103 10-11 1.5154255997654857 1103 12-13 1.5356856386493014 1103 14-15 1.3639072483801655 1103 16-17 1.3146065173469523 1103 18-19 1.4195808063085238 1103 20-21 1.633365436176959 1103 22-23 1.5303841189079606 1103 24-25 1.7835872114639102 1103 26-27 1.4159927264441876 1103 28-29 1.4591389867769422 1103 30-31 2.1415972016892546 1103 32-33 1.8700396246442672 1103 34-35 1.9418876393421982 1103 36-37 1.7692242121076411 1103 38-39 1.9204024845088163 1103 40-41 2.092648695150917 1103 42-43 2.0576171397161858 1103 44-45 1.9937794743527277 1103 46-47 1.9779430440310612 1103 48-49 1.899513475917221 1103 50-51 1.954326533766654 1103 52-53 1.827865327722698 1103 54-55 2.1242029522845343 1103 56-57 1.939138367572376 1103 58-59 2.013501653098416 1103 60-61 2.0549147587973913 1103 62-63 2.0136718612142914 1103 64-65 2.0954112902012696 1103 66-67 2.3867633974329046 1103 68-69 2.452841451015704 1103 70-71 2.2756533188776444 1103 72-73 2.429191086559765 1103 74-75 2.4203821684164843 1103 76-77 2.2373667234910357 1103 78-79 2.3702771536863665 1103 80-81 2.2444208986640994 1103 82-83 2.1630897963024864 1103 84-85 2.411936733048467 1103 86-87 2.283693993929514 1103 88-89 2.183571274984949 1103 90-91 2.3138533744574588 1103 92-93 2.0093892813391676 1103 94-95 1.9148295252298944 1103 96-97 1.8682540233951137 1103 98-99 2.0327332311829736 1103 100-101 2.0028437539377677 1103 102-103 1.875353694400502 1103 104-105 1.926599939742239 1103 106-107 1.8950849349435828 1103 108-109 1.8350486443825957 1103 110-111 1.9354011135357219 1103 112-113 1.8587440584252803 1103 114-115 1.7910632331989866 1103 116-117 1.8226150331543778 1103 118-119 1.8927985097752966 1103 120-121 1.7880622289040247 1103 122-123 1.9623891538197213 1103 124-125 1.8898352070648627 1104 1 0.6355657459684672 1104 2 0.5897527962738955 1104 3 0.4962924863705034 1104 4 0.4945751628768491 1104 5 0.8682935519298809 1104 6 1.0509769870024073 1104 7 1.3490366516768972 1104 8 1.1110227533343924 1104 9 1.7187987339022754 1104 10-11 1.2159778516941273 1104 12-13 1.4433893250489298 1104 14-15 1.3688526752013885 1104 16-17 1.192133031919667 1104 18-19 1.170707990655707 1104 20-21 1.5421128251205545 1104 22-23 1.4687554677290962 1104 24-25 1.5414961022959517 1104 26-27 1.651233207638576 1104 28-29 1.5900865752448468 1104 30-31 2.078514599105045 1104 32-33 1.8708276416654321 1104 34-35 1.825794491730214 1104 36-37 1.738740073650252 1104 38-39 1.8216309110428206 1104 40-41 1.965783314858701 1104 42-43 1.9977439664556513 1104 44-45 1.7009979502718053 1104 46-47 1.683606846369191 1104 48-49 1.5212239848642106 1104 50-51 1.8963273609845217 1104 52-53 1.6868050808213653 1104 54-55 1.9745832804996226 1104 56-57 1.8886849577787252 1104 58-59 2.018334197481373 1104 60-61 1.9594506167719246 1104 62-63 2.0583377459974024 1104 64-65 2.1398181693898586 1104 66-67 2.1788137057195627 1104 68-69 2.3000418305439005 1104 70-71 2.0863273094358377 1104 72-73 2.2996660888198157 1104 74-75 2.3879927088041555 1104 76-77 2.134267696552051 1104 78-79 2.1959657485600133 1104 80-81 2.056390477206996 1104 82-83 1.9416314128014065 1104 84-85 2.279916521411895 1104 86-87 2.2126486993511527 1104 88-89 2.024851886646161 1104 90-91 2.094440355136239 1104 92-93 1.8728460120045511 1104 94-95 1.844029675953152 1104 96-97 1.829480305882818 1104 98-99 1.8499661634143116 1104 100-101 1.9186997364269907 1104 102-103 1.8509278468021932 1104 104-105 1.761116085290979 1104 106-107 1.8897937006529233 1104 108-109 1.7788468704242781 1104 110-111 1.8731958099011585 1104 112-113 1.8894938826214833 1104 114-115 1.826553159781401 1104 116-117 1.829086132930133 1104 118-119 1.8288192878241034 1104 120-121 1.8286448254881087 1104 122-123 1.9414708619846124 1104 124-125 1.8157023657954916 1105 1 0.4667485157432445 1105 2 0.6180761844562426 1105 3 0.4996127604146636 1105 4 0.713525999675916 1105 5 0.7546584485605834 1105 6 0.9342474348355694 1105 7 1.2707962402088313 1105 8 1.156319601022897 1105 9 1.6622279023269257 1105 10-11 1.4177129289448445 1105 12-13 1.530594838078084 1105 14-15 1.5685318185120138 1105 16-17 1.403431727999866 1105 18-19 1.6560647101622763 1105 20-21 1.838589227989587 1105 22-23 1.7835295420264075 1105 24-25 1.8923865449053991 1105 26-27 1.7320567465050303 1105 28-29 1.658479278115884 1105 30-31 1.9764803636761918 1105 32-33 1.8295626988249332 1105 34-35 1.9496905799708202 1105 36-37 1.6935892890883402 1105 38-39 1.8518501061887882 1105 40-41 2.02418043779684 1105 42-43 2.0011468153898093 1105 44-45 1.8141327824097147 1105 46-47 1.9305901505702643 1105 48-49 2.011420878762511 1105 50-51 1.9832809301887124 1105 52-53 2.013781955833924 1105 54-55 2.1338091660198835 1105 56-57 1.9605669018046044 1105 58-59 1.867929560429154 1105 60-61 1.6807738241333432 1105 62-63 1.6836842189847658 1105 64-65 2.1621837089394162 1105 66-67 2.291005858091328 1105 68-69 2.3060426743748934 1105 70-71 2.157829127690718 1105 72-73 2.2826257255706643 1105 74-75 2.3543252585801397 1105 76-77 2.185837824934911 1105 78-79 2.250325943846615 1105 80-81 2.1605792944372233 1105 82-83 2.0287127621273484 1105 84-85 2.2306600972461865 1105 86-87 2.187520216036905 1105 88-89 2.131889236213194 1105 90-91 2.1539836818394704 1105 92-93 1.9875849266771652 1105 94-95 1.9614375075084105 1105 96-97 2.009944814753087 1105 98-99 2.088834224411233 1105 100-101 2.046610250159336 1105 102-103 1.8540365603617524 1105 104-105 1.5197778095189776 1105 106-107 1.7572551757498616 1105 108-109 1.797620502714878 1105 110-111 1.877816186410513 1105 112-113 1.804424266564581 1105 114-115 1.7207594563679045 1105 116-117 1.701634703440245 1105 118-119 1.7730938945385866 1105 120-121 1.7353892451533923 1105 122-123 1.748270130664423 1105 124-125 1.6563981245401926 1106 1 0.6458866919006105 1106 2 0.552366954369937 1106 3 0.6398373296561637 1106 4 0.362501732369374 1106 5 0.7838680785675152 1106 6 0.9953256863130235 1106 7 1.1870384182318148 1106 8 0.7699957819138525 1106 9 1.5333953637207713 1106 10-11 1.1874700513164598 1106 12-13 1.1151322904916192 1106 14-15 1.1932683516195084 1106 16-17 1.1221681015482403 1106 18-19 1.2282559274552014 1106 20-21 0.9447950960832827 1106 22-23 0.9682623930002308 1106 24-25 0.8717813657798175 1106 26-27 0.8649273174607757 1106 28-29 0.97126970129532 1106 30-31 1.2769136280717994 1106 32-33 1.140547158175206 1106 34-35 1.1966179007889934 1106 36-37 1.2066454395812407 1106 38-39 1.4124545488876272 1106 40-41 1.5478162584114692 1106 42-43 1.5947839247512263 1106 44-45 1.4360956051333957 1106 46-47 1.4091515409439417 1106 48-49 1.3624685889867756 1106 50-51 1.813674570734733 1106 52-53 1.4328615860658758 1106 54-55 1.8977784652075655 1106 56-57 1.5412192940663942 1106 58-59 1.5316823501568209 1106 60-61 1.8213976184902698 1106 62-63 1.9210615988193567 1106 64-65 1.984861561109934 1106 66-67 2.0809811264268205 1106 68-69 1.8719274604122411 1106 70-71 1.7888232015011312 1106 72-73 1.6291014122374534 1106 74-75 1.953282641974127 1106 76-77 1.613496785714048 1106 78-79 1.5422328595073438 1106 80-81 1.669931382104732 1106 82-83 1.4411174613481634 1106 84-85 1.7475473032636124 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-4.6632095592886635 2216 118-119 -4.909685162304854 2216 120-121 -4.83705412311167 2216 122-123 -4.92359315926889 2216 124-125 -4.844303817875385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 108363.0 3 35745.0 4 17325.0 5 14095.0 6 13851.0 7 15943.0 8 17943.0 9 19067.0 10 20167.0 11 21557.0 12 22856.0 13 24258.0 14 24500.0 15 20239.0 16 15318.0 17 13635.0 18 12178.0 19 11258.0 20 11000.0 21 11958.0 22 14457.0 23 19707.0 24 27135.0 25 35672.0 26 43688.0 27 49243.0 28 56666.0 29 64191.0 30 71385.0 31 82176.0 32 95800.0 33 113158.0 34 138757.0 35 177159.0 36 250638.0 37 330799.0 38 124126.0 39 660.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.28896211241905 19.703248845959962 14.230914329868687 24.7768747117523 2 15.648057525403312 20.80166993607305 38.9779234712012 24.572349067322445 3 19.390750244680955 24.49921343399763 29.328220926056275 26.78181539526514 4 12.86838246344061 17.367985296904152 36.71699620754962 33.04663603210562 5 13.105815371041608 38.46212254963844 34.59944761032537 13.832614468994578 6 31.82524772054244 37.70970240926308 16.886875644310987 13.578174225883496 7 27.635480583318305 32.313183379538565 21.082120416932877 18.969215620210257 8 25.552797282119823 36.59388271991123 19.36028449605506 18.493035501913894 9 25.346291680195353 16.141163558678944 19.92096607168395 38.591578689441754 10-11 24.80906429275178 26.880207205129818 27.47554928225876 20.835179219859643 12-13 25.51304689306949 24.05324456289199 27.921299152800362 22.512409391238158 14-15 22.32649768978804 25.579521159454586 25.71133029870294 26.38265085205443 16-17 22.132549885736875 28.67181081725097 26.42849872157135 22.7671405754408 18-19 21.983450862104103 27.409658128118974 28.59008565721972 22.0168053525572 20-21 22.57926864633211 26.71444280594249 28.759249765385725 21.947038782339675 22-23 22.179556040862018 26.538346689871933 28.742536588592678 22.539560680673368 24-25 22.03767729143893 26.7336308154655 28.742811501969534 22.485880391126035 26-27 21.963436316360394 26.89010234169786 28.746680480866456 22.399780861075286 28-29 21.991887198754316 26.932823070822216 28.80445074378991 22.27083898663356 30-31 22.076222361922873 26.660078116938763 28.631495488209907 22.632204032928463 32-33 21.851138948503102 26.734439758640466 28.724659042154997 22.689762250701435 34-35 22.326027521059586 26.863592383843265 28.590797648612 22.21958244648515 36-37 22.40427103391266 26.596065071729324 28.534297565994216 22.465366328363796 38-39 22.130829662711143 26.863061549294105 28.58364022459992 22.422468563394833 40-41 22.23952058987123 26.688663854827876 28.66116690378163 22.41064865151926 42-43 22.39676258959292 26.754404762015678 28.195764198608625 22.65306844978278 44-45 22.22530195269146 26.915278555797922 28.309053281795727 22.55036620971489 46-47 22.474123887638115 27.011824355012948 27.902668830016335 22.6113829273326 48-49 22.496514955122404 27.101875111364215 27.75199756832959 22.649612365183792 50-51 22.54771341427896 27.102780437907903 27.47889565377455 22.87061049403859 52-53 22.62125341487973 27.283867140361252 27.425924273928263 22.66895517083075 54-55 22.455350562456037 27.100767231455 27.450238173869725 22.993644032219244 56-57 22.37551160730174 27.36718241216845 27.36289669655177 22.89440928397804 58-59 22.42952755951362 27.11112198064679 27.554016739861343 22.905333719978245 60-61 22.484607576468328 27.21730324087553 27.579421737730897 22.718667444925238 62-63 22.433255584608457 27.1682414393716 27.75531651743477 22.64318645858518 64-65 22.332334161289765 27.044973928461395 27.535921485679182 23.086770424569657 66-67 22.231181035424935 27.24115134006627 27.422217758351135 23.105449866157663 68-69 22.428503348723282 27.112080792345616 27.547319321040938 22.91209653789016 70-71 22.423389212822894 26.941226261737384 27.54920715815865 23.086177367281067 72-73 22.397419504011108 27.092271051837873 27.413154860992307 23.097154583158716 74-75 22.185543457930102 27.34928300547223 27.32710803705176 23.138065499545903 76-77 22.36252472769546 27.05995416539821 27.47905264597114 23.09846846093519 78-79 22.360823808178406 27.126619478203267 27.476375294326182 23.036181419292145 80-81 22.320542440025147 27.130991924470944 27.353289664156655 23.195175971347258 82-83 22.523350119021554 27.14802461486861 27.38797765841979 22.94064760769005 84-85 22.257581848749204 27.23146468977809 27.207567244755023 23.303386216717684 86-87 22.357685325497318 27.192827585435854 27.354170577764904 23.095316511301927 88-89 22.394522418979232 27.062349969091244 27.300652507801725 23.242475104127795 90-91 22.21834282052793 27.226319144738664 27.350628218509126 23.204709816224277 92-93 22.343826719375837 27.19833766727444 27.376710943985483 23.08112466936424 94-95 22.22576789833361 27.031763641814145 27.441949194291688 23.300519265560553 96-97 22.262034062299175 26.928777283228953 27.4694165330655 23.339772121406366 98-99 21.9611230960654 26.902001376082897 27.395485944994885 23.74138958285682 100-101 22.032150206659935 26.856940547855544 27.59157614740228 23.51933309808224 102-103 22.006443546298314 26.737432941838517 27.638187794529507 23.61793571733366 104-105 21.92876723049435 26.769039345422307 27.50159375012374 23.800599673959603 106-107 21.778548376277325 26.783551469437345 27.43721444170325 24.000685712582083 108-109 21.947217857587066 26.73527826548338 27.313125939535272 24.004377937394285 110-111 21.791298275721914 26.84038251866324 27.351547154623223 24.01677205099162 112-113 21.924888315390813 26.865796792241003 27.37444507073747 23.83486982163071 114-115 21.84879880205127 26.937239290910874 27.293418945021582 23.920542962016277 116-117 21.65756032707357 26.861240626774546 27.473001244250987 24.008197801900895 118-119 21.652613364507513 26.743176630920246 27.343880472280397 24.26032953229185 120-121 21.508577678934955 26.855965989291064 27.45348729955679 24.18196903221719 122-123 21.49847262190783 26.932188605281944 27.329598060235643 24.23974071257458 124-125 21.464091293440205 26.8043801843479 27.287239370412557 24.444289151799335 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 202.0 1 167.0 2 256.0 3 399.5 4 293.5 5 200.0 6 370.5 7 859.5 8 1385.5 9 1547.0 10 1268.0 11 891.0 12 791.0 13 856.5 14 931.5 15 994.5 16 1169.5 17 1433.0 18 1663.0 19 1912.0 20 2294.5 21 2899.5 22 3770.5 23 4991.5 24 6456.0 25 8367.5 26 11304.0 27 14633.5 28 17715.5 29 20939.5 30 25283.5 31 30582.0 32 36448.5 33 41393.5 34 45377.5 35 49681.5 36 54764.5 37 59845.0 38 62776.5 39 64726.5 40 66551.0 41 67951.5 42 69271.5 43 71571.0 44 73894.0 45 76488.5 46 77790.5 47 77860.0 48 78831.5 49 78899.5 50 77310.0 51 74885.5 52 73245.5 53 71920.5 54 70642.0 55 66853.5 56 60870.5 57 54989.0 58 49882.5 59 44190.0 60 37395.5 61 31342.0 62 25805.5 63 20423.5 64 15012.0 65 11041.5 66 8657.5 67 7003.0 68 5675.0 69 4610.5 70 3680.0 71 2725.5 72 2054.5 73 1672.0 74 1264.0 75 868.5 76 611.5 77 389.5 78 249.5 79 182.0 80 116.0 81 71.5 82 47.0 83 32.5 84 29.0 85 23.5 86 14.0 87 10.5 88 7.5 89 5.5 90 4.5 91 3.0 92 2.0 93 1.5 94 1.0 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10481335536432423 2 1.8633485398102085E-4 3 0.0 4 0.18610193541354458 5 0.0 6 0.0 7 0.03782597535814723 8 0.0 9 0.0 10-11 3.726697079620417E-4 12-13 5.822964186906902E-4 14-15 3.959615647096694E-4 16-17 0.004308993498311107 18-19 0.00165372182908156 20-21 0.0030046495204439617 22-23 0.0015838462588386776 24-25 0.004728246919768404 26-27 0.0041692423578253415 28-29 0.0030512332339392165 30-31 9.083824131574768E-4 32-33 0.0 34-35 0.0012111765508766355 36-37 0.0012344684076242632 38-39 5.357127051954351E-4 40-41 0.0016304299723339326 42-43 8.850905564098492E-4 44-45 9.549661266527318E-4 46-47 9.316742699051042E-5 48-49 2.3291856747627606E-5 50-51 0.03670796623426111 52-53 6.987557024288283E-5 54-55 0.13327600430992517 56-57 3.260859944667865E-4 58-59 2.3291856747627606E-5 60-61 0.0 62-63 6.52171988933573E-4 64-65 0.05729796759916391 66-67 0.048982774740260866 68-69 0.0015838462588386776 70-71 0.0027717309529676853 72-73 0.07006190509686384 74-75 0.005054332914235191 76-77 0.0019332241100530915 78-79 0.0015139706885957944 80-81 1.1645928373813804E-4 82-83 1.3975114048576565E-4 84-85 0.0 86-87 1.8633485398102085E-4 88-89 0.0014906788318481668 90-91 3.493778512144141E-4 92-93 0.0023757693882580162 94-95 0.0038897400768538102 96-97 0.0022127263910246228 98-99 0.0 100-101 0.001420803261605284 102-103 0.0013509276913624014 104-105 3.027941377191589E-4 106-107 4.658371349525521E-5 108-109 0.0 110-111 3.027941377191589E-4 112-113 1.3975114048576565E-4 114-115 1.6304299723339326E-4 116-117 0.0 118-119 0.0011413009806337528 120-121 0.002562104242239037 122-123 9.549661266527318E-4 124-125 0.00875773813710798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2146673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.70266204898421 #Duplication Level Percentage of deduplicated Percentage of total 1 80.8690334626543 52.32441742362115 2 11.151497810858423 14.430631883919196 3 3.630723416126754 7.047524105609485 4 1.5754346997592574 4.077392758350644 5 0.7995127757723134 2.586530246732064 6 0.4769213042016659 1.8514846781832703 7 0.29679157265890427 1.3442243377314935 8 0.21092741191955877 1.0918052040238477 9 0.1607253809647256 0.9359424006529391 >10 0.755547192003342 8.78235381003622 >50 0.04985731692387738 2.221908688125951 >100 0.022005233735761815 2.496822245426383 >500 8.033319021600849E-4 0.35392097019449853 >1k 2.1909051877092538E-4 0.45504124739291524 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3875 0.18051188979411398 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3434 0.1599684721427064 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2321 0.10812079902248735 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.658371349525522E-5 2 0.0 0.0 0.0 0.0 4.658371349525522E-5 3 0.0 0.0 0.0 0.0 4.658371349525522E-5 4 0.0 0.0 0.0 0.0 4.658371349525522E-5 5 0.0 0.0 0.0 0.0 4.658371349525522E-5 6 0.0 0.0 0.0 0.0 4.658371349525522E-5 7 0.0 0.0 0.0 0.0 4.658371349525522E-5 8 0.0 0.0 0.0 0.0 4.658371349525522E-5 9 0.0 0.0 0.0 0.0 4.658371349525522E-5 10-11 0.0 0.0 0.0 0.0 4.658371349525522E-5 12-13 0.0 0.0 0.0 0.0 1.8633485398102085E-4 14-15 4.658371349525522E-5 0.0 0.0 0.0 3.027941377191589E-4 16-17 4.658371349525522E-5 0.0 0.0 0.0 4.192534214572969E-4 18-19 9.316742699051044E-5 0.0 0.0 0.0 4.425452782049245E-4 20-21 9.316742699051044E-5 0.0 0.0 6.987557024288283E-5 6.055882754383178E-4 22-23 1.3975114048576565E-4 0.0 0.0 2.3291856747627607E-4 7.453394159240834E-4 24-25 1.3975114048576565E-4 0.0 0.0 5.35712705195435E-4 0.0010015498401479872 26-27 1.3975114048576565E-4 4.658371349525522E-5 0.0 0.0010248416968956149 0.0010248416968956146 28-29 2.562104242239037E-4 4.658371349525522E-5 0.0 0.0017003055425768156 0.0010248416968956146 30-31 2.795022809715313E-4 9.316742699051044E-5 0.0 0.003936323790349066 0.0010481335536432423 32-33 2.795022809715313E-4 9.316742699051044E-5 0.0 0.00789593943744576 0.00107142541039087 34-35 3.260859944667865E-4 9.316742699051044E-5 0.0 0.013579152483866895 0.0011180091238861252 36-37 3.7266970796204175E-4 9.316742699051044E-5 0.0 0.02406048802029932 0.0011180091238861252 38-39 4.192534214572969E-4 9.316742699051044E-5 0.0 0.04315981055335395 0.0011180091238861252 40-41 4.192534214572969E-4 9.316742699051044E-5 0.0 0.06668458586845784 0.0011180091238861252 42-43 4.192534214572969E-4 1.3975114048576565E-4 0.0 0.0901161937565712 0.0011180091238861252 44-45 4.192534214572969E-4 1.3975114048576565E-4 0.0 0.11834592413469588 0.0012577602643718908 46-47 4.192534214572969E-4 1.3975114048576565E-4 0.0 0.1498365144574884 0.001304343977867146 48-49 4.192534214572969E-4 1.3975114048576565E-4 0.0 0.18605535170004933 0.0013975114048576564 50-51 4.192534214572969E-4 1.3975114048576565E-4 0.0 0.2277244834215551 0.0014440951183529117 52-53 4.192534214572969E-4 1.3975114048576565E-4 0.0 0.2677864770274746 0.001490678831848167 54-55 4.192534214572969E-4 1.3975114048576565E-4 0.0 0.31327547325559135 0.001490678831848167 56-57 4.6583713495255215E-4 1.3975114048576565E-4 0.0 0.36551910794052 0.001490678831848167 58-59 4.6583713495255215E-4 1.3975114048576565E-4 0.0 0.4254490553521659 0.001490678831848167 60-61 4.6583713495255215E-4 1.3975114048576565E-4 0.0 0.49520816631131054 0.001490678831848167 62-63 4.6583713495255215E-4 1.3975114048576565E-4 0.0 0.5649439854137076 0.001537262545343422 64-65 4.6583713495255215E-4 1.3975114048576565E-4 0.0 0.6367294879098959 0.001537262545343422 66-67 4.6583713495255215E-4 1.3975114048576565E-4 0.0 0.7169932262622206 0.0015605544020910497 68-69 4.6583713495255215E-4 1.3975114048576565E-4 0.0 0.8127460493517178 0.0015838462588386774 70-71 4.891289917001798E-4 1.6304299723339326E-4 0.0 0.9239879571783871 0.0015838462588386774 72-73 5.124208484478073E-4 1.8633485398102088E-4 0.0 1.0424969243103166 0.0015838462588386774 74-75 5.35712705195435E-4 1.8633485398102088E-4 0.0 1.163800914251961 0.0015838462588386774 76-77 5.590045619430626E-4 1.8633485398102088E-4 0.0 1.2976825068373246 0.0016304299723339326 78-79 5.590045619430626E-4 1.8633485398102088E-4 0.0 1.4558109222969684 0.0016537218290815603 80-81 6.055882754383178E-4 1.8633485398102088E-4 0.0 1.6325029475844715 0.0016770136858291877 82-83 6.055882754383178E-4 1.8633485398102088E-4 0.0 1.818791217851997 0.0017701811128196983 84-85 6.754638456812007E-4 1.8633485398102088E-4 0.0 2.010250280317496 0.0017701811128196983 86-87 7.453394159240835E-4 1.8633485398102088E-4 0.0 2.209838200787917 0.001840056683062581 88-89 7.453394159240835E-4 2.096267107286485E-4 0.0 2.4325316431519846 0.0019099322533054639 90-91 7.919231294193387E-4 2.3291856747627607E-4 0.0 2.6839672367426246 0.0019099322533054639 92-93 7.919231294193387E-4 2.3291856747627607E-4 0.0 2.96009219848575 0.0020962671072864846 94-95 7.919231294193387E-4 2.3291856747627607E-4 0.0 3.2347963569672697 0.00214285082078174 96-97 7.919231294193387E-4 2.3291856747627607E-4 0.0 3.5211464438226034 0.002305893818015133 98-99 8.617986996622215E-4 2.3291856747627607E-4 0.0 3.8372402317446577 0.002375769388258016 100-101 9.083824131574767E-4 2.3291856747627607E-4 0.0 4.199521771597258 0.002375769388258016 102-103 9.316742699051043E-4 2.3291856747627607E-4 0.0 4.590009749971234 0.0025388123854914093 104-105 0.0010248416968956146 2.3291856747627607E-4 0.0 4.974208927023351 0.002562104242239037 106-107 0.0010248416968956146 2.3291856747627607E-4 0.0 5.370170491733021 0.002562104242239037 108-109 0.0010248416968956146 2.3291856747627607E-4 0.0 5.785604048683707 0.0025853960989866646 110-111 0.00107142541039087 2.3291856747627607E-4 0.0 6.237908614865887 0.002608687955734292 112-113 0.0011180091238861252 2.3291856747627607E-4 0.0 6.741781351887315 0.002608687955734292 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3780 0.0 50.3564 1 GTATCAA 6220 0.0 45.95163 1 TCAACGC 7540 0.0 35.573807 4 ATCAACG 7670 0.0 35.35943 3 CAACGCA 7710 0.0 34.56542 5 TATCAAC 8260 0.0 34.42132 2 AACGCAG 7950 0.0 33.147804 6 CGCAGAG 9390 0.0 28.943928 8 ACGCAGA 9130 0.0 28.86364 7 GTACATG 6745 0.0 26.274252 1 TACATGG 6555 0.0 25.64281 2 ACATGGG 6270 0.0 25.097218 3 GCAGAGT 10600 0.0 24.966692 9 AGAGTAC 9290 0.0 24.327358 10-11 GAGTACT 6605 0.0 23.501457 12-13 GTACTTT 7070 0.0 22.376352 14-15 AGTACTT 7175 0.0 22.173231 12-13 CAGAGTA 10130 0.0 21.95782 10-11 GTGGTAT 2755 0.0 19.926088 1 TGGTATC 2565 0.0 19.520058 2 >>END_MODULE