FastQCFastQC Report
Thu 26 May 2016
SRR1364518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594100
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47310.13163239754041345No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG39620.11023622047244094No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG36950.10280737875963385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45700.061.107171
GTATCAA91350.058.142251
ATCAACG112700.044.726533
CAACGCA115500.043.642985
TCAACGC116200.043.0217364
AACGCAG117850.042.085536
TATCAAC125650.041.773972
GTACATG153900.039.6957781
TACATGG159300.036.1999242
ACGCAGA142200.034.043377
ACATGGG161650.033.39163
CGCAGAG140300.033.359898
GCAGAGT153000.028.878749
CATGGGA107050.028.6093854
CATGGGG66550.026.5018224
GAGTACT77450.024.46178412-13
AGAGTAC148850.023.5106410-11
ATGGGAG48500.023.4648425
CAGAGTA154550.023.22117610-11
AGTACAT115500.022.670982
Produced by FastQC (version 0.11.5)