##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364518_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3594100 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.457042096769705 31.0 26.0 31.0 10.0 34.0 2 26.704404997078544 31.0 26.0 31.0 10.0 34.0 3 26.727502851896162 31.0 26.0 31.0 10.0 34.0 4 29.554512951782087 35.0 28.0 37.0 10.0 37.0 5 30.194839876464204 35.0 30.0 37.0 10.0 37.0 6 30.298018140841936 35.0 30.0 37.0 10.0 37.0 7 30.115529896218803 35.0 30.0 37.0 8.0 37.0 8 29.6961731726997 35.0 29.0 37.0 8.0 37.0 9 30.369458557079657 35.0 28.0 39.0 8.0 39.0 10-11 30.46815322333825 35.0 28.5 39.0 7.0 39.0 12-13 30.29617289446593 35.0 27.5 39.0 2.0 39.0 14-15 30.97885840683342 36.0 27.5 40.0 2.0 41.0 16-17 31.240094043014942 37.0 29.0 40.0 2.0 41.0 18-19 31.199922511894492 37.0 28.5 40.0 2.0 41.0 20-21 30.976541415096964 37.0 27.5 40.0 2.0 41.0 22-23 31.03905080548677 37.0 28.0 40.0 2.0 41.0 24-25 31.068696753011878 37.0 28.0 40.0 2.0 41.0 26-27 31.18887370969088 37.0 29.0 40.0 2.0 41.0 28-29 31.13097159233188 37.0 29.0 40.0 2.0 41.0 30-31 30.76643193010768 36.0 28.0 40.0 2.0 41.0 32-33 30.770881861940403 36.0 29.0 40.0 2.0 41.0 34-35 30.46760148576834 36.0 28.0 40.0 2.0 41.0 36-37 30.222135165966446 36.0 27.0 40.0 2.0 41.0 38-39 30.081397985587493 36.0 27.0 40.0 2.0 41.0 40-41 30.09908294148744 35.5 27.0 40.0 2.0 41.0 42-43 29.962550847221834 35.0 27.0 40.0 2.0 41.0 44-45 29.612201385604187 35.0 26.5 40.0 2.0 41.0 46-47 29.34515219387329 35.0 25.5 40.0 2.0 41.0 48-49 29.083706630310786 35.0 24.5 40.0 2.0 41.0 50-51 29.055425002086753 35.0 25.5 40.0 2.0 41.0 52-53 28.85798823071144 35.0 24.5 39.0 2.0 41.0 54-55 28.74051348042625 35.0 24.5 39.0 2.0 41.0 56-57 28.325852647394342 34.0 23.0 39.0 2.0 41.0 58-59 28.101803789543975 34.0 23.0 39.0 2.0 40.0 60-61 27.698083108427703 34.0 22.0 38.5 2.0 40.0 62-63 27.656551153278983 34.0 22.0 38.0 2.0 40.0 64-65 27.48329373139312 33.5 22.0 38.0 2.0 40.0 66-67 27.245008068779388 33.0 22.0 38.0 2.0 40.0 68-69 27.075012381402853 33.0 22.0 37.0 2.0 40.0 70-71 26.924721070643553 33.0 21.5 37.0 2.0 40.0 72-73 26.89791199465791 33.0 22.0 36.5 2.0 39.0 74-75 26.622371108205115 33.0 21.5 36.0 2.0 39.0 76-77 26.32292674104783 33.0 20.0 36.0 2.0 39.0 78-79 25.996036420800756 33.0 19.5 35.0 2.0 37.5 80-81 25.99139186444451 33.0 20.0 35.0 2.0 37.0 82-83 25.788725967557944 33.0 20.0 35.0 2.0 37.0 84-85 25.608318494198826 33.0 20.0 35.0 2.0 36.0 86-87 25.389460087365403 32.5 18.5 35.0 2.0 36.0 88-89 25.164845858490303 32.0 18.0 35.0 2.0 36.0 90-91 25.010970618513674 32.0 16.5 35.0 2.0 35.0 92-93 24.91303886925795 32.0 16.0 35.0 2.0 35.0 94-95 24.84639701176929 32.0 14.5 35.0 2.0 35.0 96-97 24.7192066163991 32.0 10.0 35.0 2.0 35.0 98-99 24.626349433794275 32.0 8.5 35.0 2.0 35.0 100-101 24.573973595615037 32.0 7.0 35.0 2.0 35.0 102-103 23.44638727358727 30.0 7.0 34.0 2.0 35.0 104-105 24.218046798920454 32.0 6.0 34.5 2.0 35.0 106-107 24.310700036170388 32.0 2.0 35.0 2.0 35.0 108-109 24.25634261706686 32.0 2.0 35.0 2.0 35.0 110-111 24.158320024484574 32.0 2.0 35.0 2.0 35.0 112-113 24.14962062825186 32.0 2.0 35.0 2.0 35.0 114-115 24.110497481984364 32.0 2.0 35.0 2.0 35.0 116-117 23.922219888150025 32.0 2.0 35.0 2.0 35.0 118-119 23.777723352160486 32.0 2.0 35.0 2.0 35.0 120-121 23.645011268467766 32.0 2.0 35.0 2.0 35.0 122-123 23.382937592164936 32.0 2.0 35.0 2.0 35.0 124-125 22.40402993795387 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.051338945301147 1101 2 1.610109343181989 1101 3 1.9682842844980044 1101 4 2.323481569084567 1101 5 1.8379518798990375 1101 6 1.7208503621482976 1101 7 1.5391633703470902 1101 8 1.9026868070673935 1101 9 2.4459767941871142 1101 10-11 2.5374756050820224 1101 12-13 2.3078129250126587 1101 14-15 2.7015037143507747 1101 16-17 2.4062794906435414 1101 18-19 2.2558145321904135 1101 20-21 2.6157587511423372 1101 22-23 2.525890300755048 1101 24-25 2.3353377321288846 1101 26-27 2.093658485604198 1101 28-29 2.0082925018367703 1101 30-31 2.215625528401489 1101 32-33 2.3382179561490446 1101 34-35 2.6081861945592664 1101 36-37 2.6439142020305226 1101 38-39 2.6097865736743913 1101 40-41 2.3620127382134797 1101 42-43 2.371693308601305 1101 44-45 2.482300719075912 1101 46-47 2.4716569661304 1101 48-49 2.4630276415121024 1101 50-51 2.371352116791954 1101 52-53 2.3295965260629607 1101 54-55 2.3296721351682734 1101 56-57 2.5454196969035863 1101 58-59 2.596216177502221 1101 60-61 2.650108344414864 1101 62-63 2.2929471061582767 1101 64-65 2.2959275388834683 1101 66-67 2.4056612606775083 1101 68-69 2.290459449241226 1101 70-71 2.3056454242965927 1101 72-73 2.1965203436588894 1101 74-75 2.241049101470203 1101 76-77 2.3922700931569985 1101 78-79 2.355738416497495 1101 80-81 2.190609699675111 1101 82-83 2.2078620178657182 1101 84-85 2.061108381065246 1101 86-87 2.0550306603992645 1101 88-89 2.1913777652425814 1101 90-91 2.23096495576144 1101 92-93 2.208018371475461 1101 94-95 2.2291387668172646 1101 96-97 2.2057498836070195 1101 98-99 2.076791158506566 1101 100-101 2.1849845894946043 1101 102-103 2.213618373291389 1101 104-105 2.1683521276879354 1101 106-107 2.2961860439834965 1101 108-109 2.2587519589474567 1101 110-111 2.41775120213606 1101 112-113 2.28405417406643 1101 114-115 2.250826771381451 1101 116-117 2.3807312528965845 1101 118-119 2.415198603351037 1101 120-121 2.4050410474280675 1101 122-123 2.452850629025381 1101 124-125 2.401762509665076 1102 1 1.0132384999172643 1102 2 0.7904606116123283 1102 3 0.8493858456603895 1102 4 1.3727208417804846 1102 5 1.2880472700617887 1102 6 1.2421501751657793 1102 7 1.2271636501064265 1102 8 1.36334442826573 1102 9 1.8050710908819205 1102 10-11 1.4811614105294595 1102 12-13 1.7960300213915055 1102 14-15 2.1614275469885094 1102 16-17 1.6483054408288353 1102 18-19 1.8997272566778562 1102 20-21 1.884164819187525 1102 22-23 1.8963181407698038 1102 24-25 1.59796450434537 1102 26-27 1.6015856895174174 1102 28-29 1.3142961190297413 1102 30-31 1.2355871467240185 1102 32-33 1.2591599824188577 1102 34-35 1.6612122925206592 1102 36-37 1.8078680575328256 1102 38-39 1.846426106902456 1102 40-41 1.4825458640001479 1102 42-43 1.441167049577757 1102 44-45 1.7950578545057532 1102 46-47 1.714993833848606 1102 48-49 1.9034368659996659 1102 50-51 1.7288921009439377 1102 52-53 1.8161821003329273 1102 54-55 1.604038286619847 1102 56-57 1.6729362148624531 1102 58-59 1.794075091058339 1102 60-61 2.168894851316221 1102 62-63 2.0487287677833415 1102 64-65 2.1389237507264625 1102 66-67 2.092589395993791 1102 68-69 1.8326603680851967 1102 70-71 2.066905690599402 1102 72-73 1.9496257725693091 1102 74-75 2.0595662460047564 1102 76-77 1.9662199051842997 1102 78-79 1.8644695524833423 1102 80-81 1.4148067264550477 1102 82-83 1.3582748226962273 1102 84-85 1.4683463360595894 1102 86-87 1.6120914438808285 1102 88-89 1.4244675542022698 1102 90-91 1.3346510106358842 1102 92-93 1.28353308729287 1102 94-95 1.491273531727927 1102 96-97 1.6038557706358887 1102 98-99 1.6974635999306997 1102 100-101 1.5992299236428167 1102 102-103 1.4043105846260033 1102 104-105 1.4216973324098525 1102 106-107 1.6725002830318338 1102 108-109 1.794708867486456 1102 110-111 1.6264243432092158 1102 112-113 1.5032461941318935 1102 114-115 1.523551765195915 1102 116-117 1.8160868073352212 1102 118-119 1.9672049647424465 1102 120-121 1.9987986383442085 1102 122-123 2.0965184074178858 1102 124-125 1.9884976022651628 1103 1 1.4276520467128933 1103 2 1.1747542195637841 1103 3 1.389622723284507 1103 4 1.935191150391006 1103 5 1.354441371543178 1103 6 1.395175816780931 1103 7 1.4197826424497038 1103 8 1.758614354077551 1103 9 2.3371426645924096 1103 10-11 1.835505262372024 1103 12-13 1.9521945629814503 1103 14-15 2.203513334456016 1103 16-17 2.061313480651094 1103 18-19 1.7382979834730818 1103 20-21 2.126578864411151 1103 22-23 2.0127967588460116 1103 24-25 2.0336458637880455 1103 26-27 1.8203760251372678 1103 28-29 1.6736433214229933 1103 30-31 1.8144482893573866 1103 32-33 1.8645576208772496 1103 34-35 1.7689780574770566 1103 36-37 2.1794171714955297 1103 38-39 2.1342615118336496 1103 40-41 2.196964208899324 1103 42-43 2.1460846652651604 1103 44-45 1.954897474770842 1103 46-47 2.1552962574406784 1103 48-49 2.114714827135529 1103 50-51 2.008132512421305 1103 52-53 1.9713705589696495 1103 54-55 1.830284253526429 1103 56-57 2.0230377434832647 1103 58-59 2.218532824139338 1103 60-61 2.1794287659114744 1103 62-63 2.032515332393679 1103 64-65 1.9317209445106158 1103 66-67 1.9692447260995678 1103 68-69 1.8501155939071943 1103 70-71 1.9821526423667137 1103 72-73 1.8818642428139434 1103 74-75 1.988656944781063 1103 76-77 1.961048667865846 1103 78-79 2.1822032807272507 1103 80-81 1.6824855513994912 1103 82-83 1.825501510722784 1103 84-85 1.8111034082018236 1103 86-87 1.8411140789343214 1103 88-89 1.8121754376613275 1103 90-91 1.8232234023980425 1103 92-93 1.7908848065258418 1103 94-95 1.5805768127311097 1103 96-97 1.7765968853353016 1103 98-99 1.720333466865796 1103 100-101 1.5201330304398546 1103 102-103 1.5762471267077451 1103 104-105 1.7867364881835321 1103 106-107 1.6170609610488462 1103 108-109 1.858694264758185 1103 110-111 2.024912340828159 1103 112-113 1.9103997823011696 1103 114-115 1.828440008117898 1103 116-117 1.9775421952181595 1103 118-119 2.0832208750788865 1103 120-121 1.9469887880625336 1103 122-123 2.0170318401086114 1103 124-125 1.9801152747551143 1104 1 1.3647214236381693 1104 2 1.1802665202326637 1104 3 1.2123146486664744 1104 4 1.5514037040849296 1104 5 1.2195181930361798 1104 6 1.125725956459732 1104 7 1.1040619404337804 1104 8 1.5132617155616686 1104 9 2.0456889087437027 1104 10-11 1.833661435933763 1104 12-13 1.3576362235369217 1104 14-15 1.6854915439062843 1104 16-17 1.4451457461365464 1104 18-19 0.8818451498850877 1104 20-21 -3.684106977273668 1104 22-23 -0.33581956469836527 1104 24-25 1.1329586886180465 1104 26-27 1.2573357126291462 1104 28-29 0.8408558721891026 1104 30-31 1.5888691600537186 1104 32-33 1.4621468813560554 1104 34-35 1.6683621641731463 1104 36-37 1.7821841488188 1104 38-39 1.804744502297062 1104 40-41 1.8878301518207792 1104 42-43 1.7707630015192528 1104 44-45 1.980067798742681 1104 46-47 1.9103454684856054 1104 48-49 1.8842827315045625 1104 50-51 1.8146099795529906 1104 52-53 1.8414791355388296 1104 54-55 1.7197784633941673 1104 56-57 2.0041310735760938 1104 58-59 1.9254216754431432 1104 60-61 2.070542218351939 1104 62-63 1.779630646685849 1104 64-65 1.9158782000614742 1104 66-67 1.8627503929975688 1104 68-69 1.849239838084447 1104 70-71 2.002093805770876 1104 72-73 1.865218849115088 1104 74-75 1.8942355618655426 1104 76-77 1.8826944801826428 1104 78-79 1.8339674692062928 1104 80-81 1.5707252234916673 1104 82-83 1.6111989668003588 1104 84-85 1.543978348732498 1104 86-87 1.4051933505917553 1104 88-89 1.5109206396358346 1104 90-91 1.5306712587355804 1104 92-93 1.1989799961340388 1104 94-95 1.3809030508132523 1104 96-97 1.5221867653272554 1104 98-99 1.4426522411572051 1104 100-101 0.9406671171336605 1104 102-103 1.3363171357150563 1104 104-105 1.557128867308304 1104 106-107 1.6755063095529366 1104 108-109 1.6884730768585925 1104 110-111 1.7277798426467257 1104 112-113 1.672268000073231 1104 114-115 1.583163403986827 1104 116-117 1.6494104694762868 1104 118-119 1.667250137936616 1104 120-121 1.6416462324572514 1104 122-123 1.5179820629017833 1104 124-125 1.7337342793174422 1105 1 1.305820234393078 1105 2 0.9497530383506785 1105 3 1.0164318374675467 1105 4 1.4996367353933167 1105 5 1.167797157423479 1105 6 1.0932883211556401 1105 7 0.9795272857349531 1105 8 1.256691079831409 1105 9 1.685969503635686 1105 10-11 1.3504673955593667 1105 12-13 1.495352969131627 1105 14-15 1.7191071264401465 1105 16-17 1.4402619474063272 1105 18-19 1.1408829541547263 1105 20-21 1.3206524642243522 1105 22-23 1.2741193158975541 1105 24-25 1.349282303068101 1105 26-27 1.2402799619826048 1105 28-29 0.8326319521375432 1105 30-31 1.2301182820246765 1105 32-33 1.167578704181885 1105 34-35 0.9870378214026871 1105 36-37 1.1397381352134595 1105 38-39 1.1393077416652488 1105 40-41 1.2405958390213527 1105 42-43 1.5136134923677282 1105 44-45 1.6858573672866335 1105 46-47 1.851156255510336 1105 48-49 1.6105225231693403 1105 50-51 1.6977263822584625 1105 52-53 1.653911975979728 1105 54-55 1.6352707638767683 1105 56-57 1.9249982919002342 1105 58-59 1.5843092043819524 1105 60-61 2.0172818713652063 1105 62-63 1.9753809816220205 1105 64-65 1.6514417488499546 1105 66-67 1.834431947095446 1105 68-69 1.358057748205315 1105 70-71 1.4633676000253146 1105 72-73 1.4486979437178817 1105 74-75 1.7974169598776761 1105 76-77 1.6326941138543738 1105 78-79 1.4668139328361889 1105 80-81 1.3692776018398085 1105 82-83 1.3959718102310923 1105 84-85 1.3095885306606831 1105 86-87 1.3803937792526462 1105 88-89 1.5185433477179906 1105 90-91 1.5196815752273558 1105 92-93 1.4947453938904296 1105 94-95 1.286070112501406 1105 96-97 1.4235617164224337 1105 98-99 1.2895100402695476 1105 100-101 1.4155701919077224 1105 102-103 1.3142603313888053 1105 104-105 1.4601091436262799 1105 106-107 1.5576319662486888 1105 108-109 1.6368320951861577 1105 110-111 1.5647427713924706 1105 112-113 1.6303407581300355 1105 114-115 1.4525701731889242 1105 116-117 1.5931259555596533 1105 118-119 1.623260019290214 1105 120-121 1.6586517138943826 1105 122-123 1.7189296688987135 1105 124-125 1.380439733718049 1106 1 -0.29902251860101714 1106 2 -0.015249510240568753 1106 3 -0.07539453426834442 1106 4 -0.35984244364422224 1106 5 -0.9420053904312695 1106 6 -0.35104529051270106 1106 7 -0.2834620445517295 1106 8 0.1147436001441875 1106 9 0.13152113017699918 1106 10-11 -2.2000107146349066 1106 12-13 -0.41373528270662163 1106 14-15 -0.13914684152156198 1106 16-17 -0.3531869455905863 1106 18-19 -3.1004939847923154 1106 20-21 -5.663822579681693 1106 22-23 -6.54356439364836 1106 24-25 -6.3925318090386085 1106 26-27 -5.165899857777831 1106 28-29 -1.7580506683113981 1106 30-31 -3.6569770543141864 1106 32-33 -4.271665258756286 1106 34-35 -3.5028965166985557 1106 36-37 -6.057365907149929 1106 38-39 -6.336302593002678 1106 40-41 -2.582953374260292 1106 42-43 -1.113095099468861 1106 44-45 -0.3948184804460091 1106 46-47 -0.1703004277981286 1106 48-49 -0.06915748669973709 1106 50-51 -0.14238453132787754 1106 52-53 -0.2799876785718496 1106 54-55 -0.21248971334962619 1106 56-57 -0.08263192331379443 1106 58-59 0.05443856418381188 1106 60-61 0.22699812616050252 1106 62-63 -0.06900062519034478 1106 64-65 -0.23579485708321357 1106 66-67 -0.5613430686088492 1106 68-69 -0.4337048243589585 1106 70-71 -0.10971020826599798 1106 72-73 -0.15715180202139933 1106 74-75 -0.21469150301032158 1106 76-77 -0.2867342836202944 1106 78-79 -0.28041483859553296 1106 80-81 -0.5604555586619107 1106 82-83 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106-107 -6.347664543447664 2216 108-109 -8.602517626826561 2216 110-111 -6.071592151698532 2216 112-113 -5.527290944190987 2216 114-115 -5.933199575817444 2216 116-117 -5.411924134144321 2216 118-119 -5.735794942917789 2216 120-121 -6.591848295610426 2216 122-123 -9.397936576686114 2216 124-125 -8.077466182287907 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 314215.0 3 48538.0 4 28606.0 5 23997.0 6 24672.0 7 27086.0 8 29566.0 9 31453.0 10 32935.0 11 34733.0 12 35431.0 13 36667.0 14 38330.0 15 32411.0 16 26017.0 17 23424.0 18 21467.0 19 19735.0 20 19825.0 21 21855.0 22 26847.0 23 35993.0 24 48216.0 25 61163.0 26 74552.0 27 84233.0 28 96399.0 29 110180.0 30 123587.0 31 138860.0 32 165704.0 33 202634.0 34 251282.0 35 316159.0 36 413012.0 37 442470.0 38 131445.0 39 401.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04006029599854 18.60315941228113 13.819838408506504 23.536941883213824 2 15.286580769997249 20.485860226605997 40.12564976380828 24.10190923958847 3 18.19715341184448 25.686879756689112 29.230681867844176 26.88528496362223 4 10.927878789397246 16.687503705986455 37.98462819456032 34.39998931005598 5 13.554949333367484 38.3637680195122 33.56770542612783 14.513577220992484 6 31.46988746981401 36.726694081111994 17.824444228650833 13.97897422042316 7 28.62267668336116 33.18386199220924 20.675041736227044 17.518419588202562 8 25.764688598702506 35.1665214250815 20.423415392046547 18.645374584169446 9 26.290947348152528 16.530058408065106 20.4002222684122 36.77877197537017 10-11 24.47300464190206 27.128554573946662 27.576417110840513 20.82202367331076 12-13 25.17206180186956 24.558080570932564 28.023622089968125 22.24623553722975 14-15 22.240152001489292 25.915316159146318 25.841922792125775 26.002609047238618 16-17 22.37904002900949 29.604822188143427 25.86420056290773 22.151937219939352 18-19 22.039736551619153 27.646426035488137 28.477268303054416 21.836569109838294 20-21 23.1089751537307 27.052995474518077 28.03397162233359 21.804057749417634 22-23 22.47708917644037 27.03541881206703 27.90141267004755 22.58607934144505 24-25 22.332686290194324 27.13754858910878 28.193892545386824 22.335872575310077 26-27 22.36259176374917 27.661818280472726 27.70841231757683 22.267177638201265 28-29 22.072848032232216 27.51481669041047 28.195299283233904 22.217035994123407 30-31 22.082123333580967 27.05256163799256 28.339522800304618 22.525792228121855 32-33 22.187324569732812 27.31089356308742 27.9173753440781 22.584406523101666 34-35 22.739297310955987 27.140969155639954 27.682045062664955 22.4376884707391 36-37 22.938496256731213 26.79375773601695 27.7212380854731 22.546507921778737 38-39 22.263165371195136 26.98170831389558 27.93680059737268 22.81832571753661 40-41 22.4743422103691 26.908754737861084 28.094437699596924 22.522465352172887 42-43 22.691847302727666 27.010527396679894 27.512666171981103 22.784959128611334 44-45 22.410612839060633 27.25200827068528 27.498942209101934 22.838436681152157 46-47 22.359014654854708 27.522556444848583 27.12701435212192 22.99141454817479 48-49 22.824297291288286 27.17836013624951 27.21829111548234 22.779051456979865 50-51 22.806722431851828 27.411719036965305 26.72579891141016 23.055759619772708 52-53 23.04905830281489 27.34672263110596 26.76641181948477 22.837807246594387 54-55 22.584329490065425 27.18409076584727 26.906270204704835 23.32530953938247 56-57 22.5343126227016 27.687101548055757 26.97670354792748 22.80188228131516 58-59 22.76772974426781 27.041323684735225 27.068580895547555 23.12236567544941 60-61 22.82196538758229 27.154912258215536 27.411686593084276 22.6114357611179 62-63 22.521694016387432 27.293563717751496 27.181203244874734 23.003539020986338 64-65 22.36583802867654 27.02697889620734 27.08232407454041 23.524859000575713 66-67 22.44663110270631 27.61483735623852 26.78288444291461 23.15564709814056 68-69 22.462800258780806 27.265702499460875 27.128534758019075 23.142962483739243 70-71 22.30954254818451 27.290803258114156 27.360768653833233 23.0388855398681 72-73 22.59204838203915 27.21162773809348 27.163798833687018 23.032525046180353 74-75 22.443511079645486 27.47815796989771 26.823004884050576 23.255326066406226 76-77 22.642926201213413 27.383752048520883 26.92630528293345 23.047016467332256 78-79 22.745495192747892 27.3764011469388 27.02023006125152 22.857873599061787 80-81 22.475219507417453 28.098340658594594 26.722572563492708 22.70386727049524 82-83 22.79995536873246 27.750979230600354 26.533344203625425 22.915721197041762 84-85 22.791583071819016 27.34184174804948 26.546162093939813 23.320413086191696 86-87 22.775031177499443 27.028596486639444 26.93210066506434 23.26427167079677 88-89 22.65794973578467 27.147954741559815 26.953664441661296 23.24043108099422 90-91 22.508464012209604 27.53097094584771 27.051198665513787 22.9093663764289 92-93 22.685796224218553 27.027008602847342 26.960119054492303 23.327076118441802 94-95 22.68818606004864 27.046067731024188 26.862091191650155 23.403655017277018 96-97 22.808084127277162 27.156600368305167 26.853987466160902 23.181328038256773 98-99 22.52537239511236 27.169658023937348 26.76606477803763 23.53890480291266 100-101 22.317132568896874 27.104489970927748 26.95419683462037 23.62418062555501 102-103 22.79968820815542 26.52218678880137 27.13764597157341 23.5404790314698 104-105 22.444640947682313 26.740057658056728 27.174663399321137 23.640637994939826 106-107 22.570391703883093 27.03100693448397 27.00855326291914 23.390048098713798 108-109 22.785714573888626 27.059620270087727 26.566305547119555 23.588359608904096 110-111 22.279903177612375 27.180760953627342 27.115109932419372 23.42422593634091 112-113 22.505237644147382 26.931612137060373 27.064975725072344 23.498174493719905 114-115 21.971187776946298 27.3058077006496 26.840364713867892 23.88263980853621 116-117 22.03159355106906 27.324912949671916 27.147417650695704 23.496075848563315 118-119 22.318957039177253 27.360428696976495 26.56137984822037 23.759234415625883 120-121 22.24829468167739 27.45431371902487 26.83466846864369 23.462723130654048 122-123 22.249275583303607 27.31139984518323 26.922006741374194 23.51731783013896 124-125 22.409292852694648 27.421963583237495 26.521942231045198 23.646801333022655 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1459.0 1 1523.0 2 2001.5 3 2333.0 4 1938.5 5 1484.0 6 1312.0 7 1443.0 8 1775.0 9 1975.0 10 1947.5 11 1931.5 12 1985.5 13 2230.0 14 2670.0 15 3048.0 16 3396.0 17 3727.5 18 4136.5 19 4700.0 20 5286.5 21 6314.0 22 7875.0 23 9654.5 24 11789.5 25 15072.0 26 19842.0 27 25433.0 28 31152.0 29 36599.5 30 42314.0 31 50049.0 32 58145.5 33 64694.5 34 71567.5 35 76805.5 36 81806.5 37 88554.0 38 92587.5 39 94667.0 40 98005.0 41 106808.5 42 117637.5 43 124242.0 44 126648.5 45 127299.0 46 125963.0 47 125526.5 48 131213.0 49 133675.0 50 130928.0 51 128896.5 52 127595.0 53 126701.5 54 123962.5 55 114308.0 56 100912.0 57 90364.5 58 81861.0 59 72757.5 60 63162.5 61 54211.5 62 45978.5 63 37395.0 64 30165.0 65 24692.5 66 19310.0 67 15783.5 68 11935.5 69 8058.5 70 6407.0 71 5471.5 72 4796.5 73 4022.0 74 3137.0 75 2529.0 76 1784.5 77 1113.0 78 697.5 79 460.0 80 326.5 81 223.0 82 160.5 83 120.0 84 86.5 85 57.0 86 43.0 87 33.0 88 24.5 89 22.0 90 16.0 91 14.0 92 16.0 93 12.0 94 5.5 95 5.0 96 5.5 97 3.5 98 2.5 99 3.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10606271389221225 2 0.03494616176511505 3 0.04615898277732951 4 0.05394952839375643 5 0.058178681728388186 6 0.003644862413399739 7 0.002782337720152472 8 0.002448457193734175 9 0.006872374168776607 10-11 0.05670404273670738 12-13 0.00724798976099719 14-15 0.012548343117887651 16-17 0.022077849809409866 18-19 0.0211040316073565 20-21 0.13547202359422386 22-23 0.01826604713280098 24-25 0.015956706825074427 26-27 0.00813833783144598 28-29 0.005008207896274449 30-31 0.0025319273253387497 32-33 0.007164519629392615 34-35 0.0195876575498734 36-37 0.003213600066776105 38-39 0.010113797612754237 40-41 0.034334047466681505 42-43 0.01590106007067138 44-45 0.11326896858740713 46-47 0.00477170919006149 48-49 0.011101527503408364 50-51 0.012798753512701372 52-53 0.014148187306975321 54-55 0.011504966472830473 56-57 0.0074984001558109125 58-59 0.015455886035446982 60-61 0.034960073453715816 62-63 0.008611335243871901 64-65 0.008096602765643694 66-67 0.010767646976990067 68-69 0.009042597590495534 70-71 0.005022119584875212 72-73 0.001085111710859464 74-75 0.0015163740574830973 76-77 0.009696446954731366 78-79 0.024568042068946326 80-81 0.00400656631701956 82-83 0.005759439080715617 84-85 0.0035753039703959265 86-87 0.0045073871066470044 88-89 0.006983667677582704 90-91 0.00577335076931638 92-93 0.003213600066776105 94-95 0.004549122172449292 96-97 0.0019337247155059682 98-99 0.003756155922205837 100-101 0.0034361870843883035 102-103 9.877298906541276E-4 104-105 0.004256976711833282 106-107 9.181714476503158E-4 108-109 0.0012659636626693748 110-111 0.0026849558999471356 112-113 0.004604768926852342 114-115 0.0029075429175593336 116-117 0.005620322194707993 118-119 0.0038813611196126985 120-121 0.0074984001558109125 122-123 0.004841267633065301 124-125 0.054617289446593026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3594100.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.374809927817125 #Duplication Level Percentage of deduplicated Percentage of total 1 77.55829482967464 39.84542655200136 2 10.942019052558605 11.24288298103504 3 4.36292674376818 6.72433596570241 4 2.202920252606624 4.526984370552168 5 1.314076585041959 3.3755217393562833 6 0.8118960525600942 2.502660322885192 7 0.5487875546139711 1.9735699416331032 8 0.396882517554772 1.6311811122439963 9 0.2879405190960508 1.3313600495168947 >10 1.4065921919974427 12.750472392510897 >50 0.09204029280094685 3.2908739987805684 >100 0.06741465863684892 6.7995210490429585 >500 0.00635889014245706 2.1891874587472295 >1k 0.0018498589473783808 1.816022065991751 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4731 0.13163239754041345 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3962 0.11023622047244094 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 3695 0.10280737875963385 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.5646754403049445E-5 0.0 14-15 0.0 1.3911688600762361E-5 0.0 8.347013160457417E-5 0.0 16-17 0.0 2.7823377201524723E-5 0.0 1.2520519740686123E-4 0.0 18-19 0.0 2.7823377201524723E-5 0.0 2.2258701761219778E-4 0.0 20-21 2.7823377201524723E-5 2.7823377201524723E-5 0.0 3.895272808213461E-4 0.0 22-23 2.7823377201524723E-5 2.7823377201524723E-5 0.0 8.625246932472664E-4 0.0 24-25 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.0014189922372777608 0.0 26-27 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.0017806961408975822 0.0 28-29 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.002629309145544086 0.0 30-31 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.005662057260510281 0.0 32-33 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.011184997635012938 0.0 34-35 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.018836426365432236 0.0 36-37 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.03240032275117554 0.0 38-39 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.05605019337247155 0.0 40-41 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.08558470827189005 0.0 42-43 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.11374196599983306 0.0 44-45 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.14664310954063603 0.0 46-47 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.19014495979521995 0.0 48-49 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.2374725244150135 0.0 50-51 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.29238195932222255 0.0 52-53 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.3454967863999332 0.0 54-55 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.40037839792994073 0.0 56-57 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.46534598369550095 0.0 58-59 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.5441696113074206 0.0 60-61 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.6359311093180491 0.0 62-63 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.728777719039537 0.0 64-65 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.8237806404941432 0.0 66-67 2.7823377201524723E-5 2.7823377201524723E-5 0.0 0.9344202999360063 0.0 68-69 2.7823377201524723E-5 2.7823377201524723E-5 0.0 1.0656353468183968 0.0 70-71 2.7823377201524723E-5 2.7823377201524723E-5 0.0 1.220235942238669 0.0 72-73 2.7823377201524723E-5 2.7823377201524723E-5 0.0 1.3765198519796333 0.0 74-75 2.7823377201524723E-5 2.7823377201524723E-5 0.0 1.539036198213739 0.0 76-77 2.7823377201524723E-5 2.7823377201524723E-5 0.0 1.7176344564703263 0.0 78-79 2.7823377201524723E-5 5.5646754403049445E-5 0.0 1.9237639464678222 0.0 80-81 2.7823377201524723E-5 5.5646754403049445E-5 0.0 2.161389499457444 0.0 82-83 2.7823377201524723E-5 5.5646754403049445E-5 0.0 2.411966834534376 0.0 84-85 2.7823377201524723E-5 5.5646754403049445E-5 0.0 2.662808491694722 0.0 86-87 2.7823377201524723E-5 5.5646754403049445E-5 0.0 2.9249186166216856 0.0 88-89 2.7823377201524723E-5 5.5646754403049445E-5 0.0 3.2139478589911246 0.0 90-91 2.7823377201524723E-5 5.5646754403049445E-5 0.0 3.5473414763083944 0.0 92-93 2.7823377201524723E-5 5.5646754403049445E-5 0.0 3.904593639575972 0.0 94-95 2.7823377201524723E-5 5.5646754403049445E-5 0.0 4.26551848863415 0.0 96-97 2.7823377201524723E-5 5.5646754403049445E-5 0.0 4.630964636487577 0.0 98-99 2.7823377201524723E-5 5.5646754403049445E-5 0.0 5.02281516930525 0.0 100-101 2.7823377201524723E-5 5.5646754403049445E-5 0.0 5.47006204613116 0.0 102-103 2.7823377201524723E-5 8.347013160457416E-5 0.0 5.9509056509279095 0.0 104-105 2.7823377201524723E-5 8.347013160457416E-5 0.0 6.434044684343785 0.0 106-107 2.7823377201524723E-5 8.347013160457416E-5 0.0 6.916112517737403 0.0 108-109 2.7823377201524723E-5 8.347013160457416E-5 0.0 7.418853120391754 1.3911688600762361E-5 110-111 2.7823377201524723E-5 8.347013160457416E-5 0.0 7.980982721682758 2.7823377201524723E-5 112-113 2.7823377201524723E-5 8.347013160457416E-5 0.0 8.597854817617762 2.7823377201524723E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4570 0.0 61.10717 1 GTATCAA 9135 0.0 58.14225 1 ATCAACG 11270 0.0 44.72653 3 CAACGCA 11550 0.0 43.64298 5 TCAACGC 11620 0.0 43.021736 4 AACGCAG 11785 0.0 42.08553 6 TATCAAC 12565 0.0 41.77397 2 GTACATG 15390 0.0 39.695778 1 TACATGG 15930 0.0 36.199924 2 ACGCAGA 14220 0.0 34.04337 7 ACATGGG 16165 0.0 33.3916 3 CGCAGAG 14030 0.0 33.35989 8 GCAGAGT 15300 0.0 28.87874 9 CATGGGA 10705 0.0 28.609385 4 CATGGGG 6655 0.0 26.501822 4 GAGTACT 7745 0.0 24.461784 12-13 AGAGTAC 14885 0.0 23.51064 10-11 ATGGGAG 4850 0.0 23.464842 5 CAGAGTA 15455 0.0 23.221176 10-11 AGTACAT 11550 0.0 22.67098 2 >>END_MODULE