FastQCFastQC Report
Thu 26 May 2016
SRR1364518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364518_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594100
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117990.3282880276007902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101760.2831306864027156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68810.19145265852369162No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC46120.12832141565343202No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG42980.11958487521215326No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG36160.1006093319607134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72050.070.792421
GTATCAA139450.056.6401141
ATCAACG182500.041.0118063
TATCAAC192450.040.5325432
CAACGCA184200.040.1427845
TCAACGC186000.039.855624
AACGCAG188150.038.667696
GTACATG146950.034.7909161
GTGGTAT30950.034.694971
ACGCAGA218650.032.9201557
TACATGG152350.032.8563232
CGCAGAG221550.032.8009578
TGGTATC32750.031.1147962
ACATGGG154800.030.4885223
GCAGAGT235550.029.6648249
GAGTACT156350.026.91236312-13
AGTACTT156950.026.5252812-13
GTACTTT169550.025.53623414-15
CATGGGG62800.025.5255324
AGAGTAC230150.025.47939910-11
Produced by FastQC (version 0.11.5)