FastQCFastQC Report
Thu 26 May 2016
SRR1364517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959715
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57250.047.9281161
GGTATCA33350.046.7595561
ATCAACG68100.038.2681733
GTACATG98300.037.843511
CAACGCA70250.037.3143045
TCAACGC70400.036.8969274
AACGCAG72900.035.1234256
TACATGG102050.034.6313552
TATCAAC79100.034.073582
ACATGGG102850.031.7598763
ACGCAGA85150.029.5814177
CGCAGAG85200.028.5870298
GCAGAGT95250.023.5075939
CATGGGG57800.023.500844
CATGGGA61800.023.3293844
GTATAGG9700.022.091
GAGTACA80900.021.5567861
AGTACAT74350.021.286132
AGAGTAC89250.020.839210-11
CAGAGTA91500.019.8714410-11
Produced by FastQC (version 0.11.5)