##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364517_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2959715 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.4553587085243 30.0 25.0 31.0 2.0 34.0 2 25.661464701837847 30.0 25.0 31.0 2.0 34.0 3 25.67452339161034 30.0 25.0 31.0 2.0 34.0 4 28.440914750237777 35.0 26.0 37.0 2.0 37.0 5 28.968960524915406 35.0 28.0 37.0 2.0 37.0 6 29.180473119878098 35.0 28.0 37.0 2.0 37.0 7 28.904452962531867 35.0 28.0 37.0 2.0 37.0 8 28.566703550848647 35.0 27.0 37.0 2.0 37.0 9 29.2100202215416 35.0 27.0 39.0 2.0 39.0 10-11 29.26061208596098 35.0 27.0 39.0 2.0 39.0 12-13 29.112061803247947 35.0 26.5 39.0 2.0 39.0 14-15 29.715139295506493 36.0 26.5 40.0 2.0 41.0 16-17 29.938686833022775 36.0 27.0 40.0 2.0 41.0 18-19 29.85715161763886 36.0 27.0 40.0 2.0 41.0 20-21 29.644413904717176 36.0 26.0 40.0 2.0 41.0 22-23 29.660079771194184 36.0 26.0 40.0 2.0 41.0 24-25 29.671464144351738 36.0 26.0 40.0 2.0 41.0 26-27 29.775451690449927 36.0 26.5 40.0 2.0 41.0 28-29 29.689352353182656 36.0 26.5 40.0 2.0 41.0 30-31 29.347200152717406 36.0 25.0 40.0 2.0 41.0 32-33 29.362804526787208 35.0 25.0 40.0 2.0 41.0 34-35 29.068187139640134 35.0 25.0 40.0 2.0 41.0 36-37 28.828194944445663 35.0 24.0 40.0 2.0 41.0 38-39 28.673310268049455 35.0 24.0 40.0 2.0 41.0 40-41 28.70145622129158 35.0 24.0 40.0 2.0 41.0 42-43 28.557032687268876 35.0 24.0 40.0 2.0 41.0 44-45 28.210128340059768 34.5 22.5 39.0 2.0 41.0 46-47 27.975731616050872 34.0 22.0 39.0 2.0 41.0 48-49 27.73911525265102 34.0 21.0 39.0 2.0 40.0 50-51 27.71547395610726 34.0 21.5 39.0 2.0 40.0 52-53 27.520610599331356 34.0 19.5 39.0 2.0 40.0 54-55 27.380761999043827 33.5 19.5 39.0 2.0 40.0 56-57 26.96550917909326 33.0 17.0 38.5 2.0 40.0 58-59 26.72651809380295 33.0 15.0 38.0 2.0 40.0 60-61 26.32220230664101 33.0 13.0 38.0 2.0 40.0 62-63 26.246796904431676 33.0 13.0 38.0 2.0 40.0 64-65 26.047974551603787 32.5 12.0 37.0 2.0 40.0 66-67 25.79431313487954 32.0 12.0 37.0 2.0 40.0 68-69 25.622856592611114 32.0 10.0 36.0 2.0 39.0 70-71 25.493273845623648 32.0 8.0 36.0 2.0 39.0 72-73 25.468561331074106 32.0 9.5 36.0 2.0 39.0 74-75 25.20583586595331 32.0 7.5 35.5 2.0 39.0 76-77 24.9241433719125 32.0 7.0 35.0 2.0 37.5 78-79 24.633712198640747 32.0 6.5 35.0 2.0 37.0 80-81 24.642699550463476 32.0 3.5 35.0 2.0 37.0 82-83 24.469719888570353 32.0 2.0 35.0 2.0 36.0 84-85 24.307098825393663 32.0 2.0 35.0 2.0 36.0 86-87 24.121718983077763 32.0 2.0 35.0 2.0 35.5 88-89 23.917726368924036 32.0 2.0 35.0 2.0 35.0 90-91 23.773186269623935 31.5 2.0 35.0 2.0 35.0 92-93 23.681206974320162 31.0 2.0 35.0 2.0 35.0 94-95 23.62695580486635 31.5 2.0 35.0 2.0 35.0 96-97 23.50719444270817 31.0 2.0 35.0 2.0 35.0 98-99 23.423880846635573 31.0 2.0 35.0 2.0 35.0 100-101 23.38100982695969 31.0 2.0 35.0 2.0 35.0 102-103 22.278001598126846 29.5 2.0 33.5 2.0 34.5 104-105 23.04159201139299 31.0 2.0 34.0 2.0 35.0 106-107 23.13653848427974 31.0 2.0 35.0 2.0 35.0 108-109 23.091664400119605 31.0 2.0 35.0 2.0 35.0 110-111 22.994954412840425 31.0 2.0 35.0 2.0 35.0 112-113 22.98538609291773 31.0 2.0 35.0 2.0 35.0 114-115 22.946504139756698 31.0 2.0 35.0 2.0 35.0 116-117 22.754502882878924 31.0 2.0 35.0 2.0 35.0 118-119 22.615802703976563 31.0 2.0 35.0 2.0 35.0 120-121 22.47343882772497 31.0 2.0 35.0 2.0 35.0 122-123 22.2139724601862 31.0 2.0 35.0 2.0 35.0 124-125 21.248794394054833 29.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.733067023294204 1101 2 1.2666776611575763 1101 3 1.6151313676095391 1101 4 2.0785315456446156 1101 5 1.5136684779046554 1101 6 1.5458653922124483 1101 7 1.4263800154832857 1101 8 1.7946537791867812 1101 9 2.235465176066043 1101 10-11 2.190703527477357 1101 12-13 1.9082924510432449 1101 14-15 2.334402417622897 1101 16-17 2.0015826585675676 1101 18-19 1.885065697850571 1101 20-21 2.36811690303616 1101 22-23 2.216511654053811 1101 24-25 2.184720668529799 1101 26-27 1.8468014386020961 1101 28-29 1.7634745993076884 1101 30-31 1.9359340103445 1101 32-33 2.116217972043369 1101 34-35 2.2482804555991187 1101 36-37 2.2809948220203893 1101 38-39 2.3896628120808536 1101 40-41 2.181975804931657 1101 42-43 2.1898867052009514 1101 44-45 2.3207526928304247 1101 46-47 2.3819670397454438 1101 48-49 2.3133801583351534 1101 50-51 2.2519113349923465 1101 52-53 2.1526572845634533 1101 54-55 2.1465191146118414 1101 56-57 2.437193653286947 1101 58-59 2.448916206539206 1101 60-61 2.5091913136392776 1101 62-63 2.148203723741979 1101 64-65 2.2298776779612837 1101 66-67 2.2772654020933842 1101 68-69 2.1480503824040014 1101 70-71 2.1969958298503798 1101 72-73 2.104995492934478 1101 74-75 1.9416215102135013 1101 76-77 2.201651526416317 1101 78-79 2.1305834144554474 1101 80-81 2.011029435860465 1101 82-83 2.003357569767253 1101 84-85 1.854670553475831 1101 86-87 1.7773709136369291 1101 88-89 1.970004178574161 1101 90-91 1.9187690616264774 1101 92-93 1.8620970595329958 1101 94-95 1.9011727906168367 1101 96-97 1.9370798832724994 1101 98-99 1.7985759070948788 1101 100-101 1.8432298561055624 1101 102-103 1.8838193898330573 1101 104-105 1.7755849113266287 1101 106-107 1.8941048337441728 1101 108-109 1.961315255592023 1101 110-111 2.033718728729351 1101 112-113 1.8940217086287277 1101 114-115 1.885396879336362 1101 116-117 1.9297305530426883 1101 118-119 1.9628510192423505 1101 120-121 1.941012744643782 1101 122-123 1.8691020145892097 1101 124-125 1.932163614000224 1102 1 0.6626573425022428 1102 2 0.3663487235750509 1102 3 0.5099181564272648 1102 4 0.9429305896814242 1102 5 0.9501135441066992 1102 6 0.8087198788317309 1102 7 0.701797535960516 1102 8 0.9673015728513761 1102 9 1.2498602986282137 1102 10-11 0.9116292202424567 1102 12-13 1.2937350792164821 1102 14-15 1.5494451756850722 1102 16-17 1.0143607440430316 1102 18-19 1.21174199769818 1102 20-21 1.3438610902776666 1102 22-23 1.2365193580495273 1102 24-25 0.938294316294833 1102 26-27 0.9998273109157125 1102 28-29 0.7831189257545539 1102 30-31 0.6627935097367903 1102 32-33 0.6295179027437818 1102 34-35 1.0623135901480332 1102 36-37 1.1478937245390597 1102 38-39 1.2598419045686242 1102 40-41 0.7663720008867685 1102 42-43 0.9292576670962482 1102 44-45 1.3161035771486027 1102 46-47 1.144849317076794 1102 48-49 1.2297896132576405 1102 50-51 1.0448387293323869 1102 52-53 1.0494519756421745 1102 54-55 0.9164757135397359 1102 56-57 1.0253882940253156 1102 58-59 1.0328525734556848 1102 60-61 1.5812067007410455 1102 62-63 1.2980013737854819 1102 64-65 1.4152914802650578 1102 66-67 1.3555873457422116 1102 68-69 1.2372972699608233 1102 70-71 1.4867195286225652 1102 72-73 1.2167950096185542 1102 74-75 1.3948727997072012 1102 76-77 1.2166214644197133 1102 78-79 1.1898330027472355 1102 80-81 0.7910755787008092 1102 82-83 0.7933111014584995 1102 84-85 0.7956134018764693 1102 86-87 0.9961705703004 1102 88-89 0.7672680197709454 1102 90-91 0.7354185331786276 1102 92-93 0.6802594462590328 1102 94-95 0.8637152005956779 1102 96-97 0.9318287209819225 1102 98-99 1.1414573861426902 1102 100-101 0.9787182736735076 1102 102-103 0.6609527070079864 1102 104-105 0.7000352777210601 1102 106-107 1.0220920438616368 1102 108-109 1.1784659111870432 1102 110-111 0.9754122294423482 1102 112-113 0.8990079498948198 1102 114-115 0.935858540883352 1102 116-117 1.1150421685251715 1102 118-119 1.2052405140920683 1102 120-121 1.1529067124459083 1102 122-123 1.3237969756201977 1102 124-125 1.2287671693275684 1103 1 1.4282890818082024 1103 2 1.048828787236296 1103 3 1.2935148658204412 1103 4 1.7684915540638961 1103 5 1.2554070592559476 1103 6 1.26066227707879 1103 7 1.2771482747971135 1103 8 1.6216375720724905 1103 9 2.2725100087328514 1103 10-11 1.7766854260250327 1103 12-13 1.9800668143351885 1103 14-15 2.143600481188461 1103 16-17 2.0563079795526242 1103 18-19 1.6855287582589078 1103 20-21 2.0729158927225413 1103 22-23 1.9418326391095881 1103 24-25 2.0170717525121162 1103 26-27 1.7427180877285977 1103 28-29 1.599930082363887 1103 30-31 1.841743313649065 1103 32-33 1.8775313797470154 1103 34-35 1.7843781192488741 1103 36-37 2.034730877587638 1103 38-39 2.0619423321818857 1103 40-41 2.2236512416818144 1103 42-43 2.2521893783224023 1103 44-45 2.084591884579531 1103 46-47 2.165591539850684 1103 48-49 2.2395009750053276 1103 50-51 2.1283583987683947 1103 52-53 2.2406387621471175 1103 54-55 2.168514484007762 1103 56-57 2.265229856191599 1103 58-59 2.2815830135272073 1103 60-61 2.2778715914755203 1103 62-63 2.1827227723633236 1103 64-65 2.0713847291084093 1103 66-67 2.0579431541455833 1103 68-69 1.996819062681837 1103 70-71 2.196890588848486 1103 72-73 2.2353890942815617 1103 74-75 2.278497957277274 1103 76-77 2.3093130713542216 1103 78-79 2.3736901288313668 1103 80-81 1.9729321931747172 1103 82-83 2.1459591273340806 1103 84-85 2.0751504524444684 1103 86-87 2.0158470239294957 1103 88-89 2.018836003453135 1103 90-91 2.0119073483029695 1103 92-93 2.0171170553233573 1103 94-95 1.8436059625806323 1103 96-97 2.0612642655078197 1103 98-99 2.016109058010475 1103 100-101 1.8637518514749587 1103 102-103 1.9091824712895935 1103 104-105 2.092044604022906 1103 106-107 1.8544289760300074 1103 108-109 2.172744428397749 1103 110-111 2.2880862303079645 1103 112-113 2.1906960929688637 1103 114-115 2.111559792407295 1103 116-117 2.2746053162504367 1103 118-119 2.4566418001305834 1103 120-121 2.4431175646816676 1103 122-123 2.407620221282535 1103 124-125 2.353127621577574 1104 1 1.120176491290927 1104 2 0.9221769151256964 1104 3 1.0159890039010868 1104 4 1.2767660332766866 1104 5 0.8973995539927877 1104 6 0.8085107104563427 1104 7 0.7393423694641754 1104 8 1.1368121675621232 1104 9 1.587350146983482 1104 10-11 1.3688776116692694 1104 12-13 0.9175953551164078 1104 14-15 1.3722216727658 1104 16-17 0.9695296758417733 1104 18-19 0.3164886585447242 1104 20-21 -3.995065678309796 1104 22-23 -0.8021834420714953 1104 24-25 0.6506690190050293 1104 26-27 0.8330749192490998 1104 28-29 0.3757600956736482 1104 30-31 1.1423843715236082 1104 32-33 1.0273447716533788 1104 34-35 1.2834594075767356 1104 36-37 1.2903299881342996 1104 38-39 1.3558239140275674 1104 40-41 1.3772370006385373 1104 42-43 1.3297364219402361 1104 44-45 1.4546786093950033 1104 46-47 1.3702670215452066 1104 48-49 1.5035712519649351 1104 50-51 1.3741352753845568 1104 52-53 1.4394702977802076 1104 54-55 1.3536339180826147 1104 56-57 1.7274116912831765 1104 58-59 1.723723446408144 1104 60-61 1.7649277714807994 1104 62-63 1.3699148756837332 1104 64-65 1.5203577949768174 1104 66-67 1.3524622784158495 1104 68-69 1.2973894333126097 1104 70-71 1.5822761291353196 1104 72-73 1.3773966520539744 1104 74-75 1.4043823265632795 1104 76-77 1.4136983059304065 1104 78-79 1.391623017556693 1104 80-81 1.199884302511439 1104 82-83 1.1925925945066744 1104 84-85 1.1775568224397936 1104 86-87 0.9858680676799381 1104 88-89 1.1698394419191906 1104 90-91 1.223133848743732 1104 92-93 0.858105021359659 1104 94-95 0.980955526909586 1104 96-97 1.0252996327143684 1104 98-99 0.9812755323657143 1104 100-101 0.561425491340696 1104 102-103 1.052894666296499 1104 104-105 1.105818225955396 1104 106-107 1.2248007415379263 1104 108-109 1.2059897716502874 1104 110-111 1.3492314304060393 1104 112-113 1.323066200430386 1104 114-115 1.265288194083496 1104 116-117 1.355002789042409 1104 118-119 1.2760953879587618 1104 120-121 1.2662955492430576 1104 122-123 1.1000248914020574 1104 124-125 1.2147554173059056 1105 1 1.4263411615998436 1105 2 1.1647963158956323 1105 3 1.1763180245077152 1105 4 1.799655652595181 1105 5 1.4799307153107044 1105 6 1.501821976350648 1105 7 1.3197485018606265 1105 8 1.497195144602685 1105 9 2.054445131671585 1105 10-11 1.7107743962385484 1105 12-13 1.8949903821241705 1105 14-15 2.153033410267522 1105 16-17 1.9554526831470334 1105 18-19 1.5238895084835633 1105 20-21 1.8062627090928558 1105 22-23 1.6976290740938396 1105 24-25 1.741514084945976 1105 26-27 1.6422203075195085 1105 28-29 1.1967151700103429 1105 30-31 1.6740243425589583 1105 32-33 1.5348322609882352 1105 34-35 1.6199377979567622 1105 36-37 1.65884529526096 1105 38-39 1.667367568055461 1105 40-41 1.7476047589646235 1105 42-43 1.9452072816021406 1105 44-45 2.1155224662027194 1105 46-47 2.4659724394166496 1105 48-49 1.9887176411794663 1105 50-51 2.0465886067057006 1105 52-53 2.0841779534572495 1105 54-55 2.1726097867834717 1105 56-57 2.421806608741651 1105 58-59 2.133099070009518 1105 60-61 2.510658940253524 1105 62-63 2.2887661718427346 1105 64-65 2.0139650257753487 1105 66-67 2.2789560011506893 1105 68-69 1.8528078956860696 1105 70-71 1.9409892522764487 1105 72-73 1.8048833640888944 1105 74-75 2.172722513924384 1105 76-77 2.067953977606191 1105 78-79 1.9231444844444 1105 80-81 1.8915912395269636 1105 82-83 1.9455990406413797 1105 84-85 1.910620684220408 1105 86-87 1.9126634146079908 1105 88-89 2.025691942211246 1105 90-91 2.043709364341325 1105 92-93 2.0070506890034814 1105 94-95 1.8034542689110111 1105 96-97 1.9292417479611288 1105 98-99 1.7729021938443204 1105 100-101 1.8773293888862028 1105 102-103 1.8966594935199943 1105 104-105 2.0010905150309704 1105 106-107 2.054988457198885 1105 108-109 2.15196597172638 1105 110-111 2.1135173167757024 1105 112-113 2.0646050406534293 1105 114-115 2.0635622700397924 1105 116-117 2.1526295468880257 1105 118-119 2.1774703679012433 1105 120-121 2.1667170639960744 1105 122-123 2.2735876933728534 1105 124-125 1.9224414135885013 1106 1 -0.19472252766197684 1106 2 -0.12267212149451368 1106 3 -0.03090848524955092 1106 4 -0.3921358697445818 1106 5 -0.921490184280966 1106 6 -0.38605176109292927 1106 7 -0.21103491961160614 1106 8 0.103135235061238 1106 9 -0.0403194466502832 1106 10-11 -2.3848747468137894 1106 12-13 -0.4155224082799336 1106 14-15 -0.17131025139712008 1106 16-17 -0.41135593596771614 1106 18-19 -3.2595755294845254 1106 20-21 -5.733994848555781 1106 22-23 -6.400212230003341 1106 24-25 -6.208426437655046 1106 26-27 -5.108104342744092 1106 28-29 -1.8927263186908476 1106 30-31 -3.728545383539771 1106 32-33 -4.263467001275579 1106 34-35 -3.5636920405739296 1106 36-37 -5.845905543205955 1106 38-39 -6.3763843127979065 1106 40-41 -2.574441689043077 1106 42-43 -1.1868876718997647 1106 44-45 -0.3420944355254285 1106 46-47 -0.15033167048295226 1106 48-49 -0.009806650577928622 1106 50-51 -0.018821108424823763 1106 52-53 -0.2120979996599175 1106 54-55 -0.1926259336575349 1106 56-57 -0.0381508180109158 1106 58-59 0.0890231516801876 1106 60-61 0.2721215029295614 1106 62-63 -0.014209469409465214 1106 64-65 -0.08154067872254345 1106 66-67 -0.44646438157195334 1106 68-69 -0.3765261419753507 1106 70-71 -0.0015329557255014947 1106 72-73 -0.008852203663934688 1106 74-75 -0.12516107020712752 1106 76-77 -0.18955816287304117 1106 78-79 -0.24830332091796947 1106 80-81 -0.5156079468390544 1106 82-83 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78-79 -4.4603602950174555 2216 80-81 -4.48475440925084 2216 82-83 -4.786361716314559 2216 84-85 -4.5242469407468455 2216 86-87 -4.780368445386692 2216 88-89 -5.089804749102356 2216 90-91 -5.0645289883427616 2216 92-93 -4.839963366070808 2216 94-95 -7.868069568321957 2216 96-97 -9.827368867393872 2216 98-99 -6.756410073340064 2216 100-101 -5.918278951363732 2216 102-103 -6.68014190557413 2216 104-105 -9.336223222792128 2216 106-107 -6.19580788427281 2216 108-109 -8.414371917727479 2216 110-111 -6.024013156108214 2216 112-113 -5.469380825553181 2216 114-115 -5.831431600855506 2216 116-117 -5.3679588480751015 2216 118-119 -5.620732580331904 2216 120-121 -6.372327245102973 2216 122-123 -9.113635025205634 2216 124-125 -7.796902339868565 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 334931.0 3 48616.0 4 28293.0 5 23301.0 6 23386.0 7 25198.0 8 26721.0 9 28374.0 10 29275.0 11 29896.0 12 29819.0 13 29546.0 14 28970.0 15 24752.0 16 20914.0 17 18895.0 18 17407.0 19 16510.0 20 16710.0 21 18458.0 22 22903.0 23 31230.0 24 41757.0 25 52987.0 26 64126.0 27 72693.0 28 82418.0 29 93013.0 30 104532.0 31 118449.0 32 139365.0 33 167868.0 34 204214.0 35 254513.0 36 321721.0 37 298728.0 38 69130.0 39 96.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67262390912119 18.597148031472095 13.519270994502698 24.21095706490401 2 15.318010017037547 20.236119264867465 39.6508806563833 24.794990061711683 3 18.8238443654683 25.133872224952658 28.538969138235366 27.50331427134367 4 11.47690664286849 16.213656231302036 37.01606714871493 35.293369977114544 5 13.975898889869617 37.95842022689403 33.528310708047435 14.537370175188913 6 32.53919851061709 36.31215546616705 17.098710474166403 14.049935549049456 7 29.132553576599324 31.94735442575637 20.609965870860687 18.310126126783622 8 26.199569948277563 35.566338383940774 19.497682829421375 18.736408838360287 9 26.151619171706642 16.45997120466348 19.994918072503292 37.39349155112659 10-11 24.844453399577276 26.714308799725217 27.10194134057437 21.33929646012314 12-13 25.525397522099013 24.005877978226884 27.569545882593836 22.899178617080267 14-15 22.74973769622097 25.404961554122433 24.876045624806757 26.969255124849838 16-17 23.047889820918606 28.831169621166897 25.174552102203613 22.946388455710885 18-19 22.918251099943785 27.503773492769916 27.108555779176204 22.469419628110103 20-21 23.440142609346406 26.868558989933916 27.13109659861552 22.560201802104157 22-23 23.412754600146865 26.63886409802112 26.88997934914144 23.058401952690577 24-25 23.18865777114545 26.916622567535875 26.885144912109247 23.009574749209428 26-27 22.98977460336125 27.319197133023415 26.57023464146236 23.120793622152977 28-29 22.94182193235352 27.03784577359437 26.94594076350425 23.074391530547857 30-31 22.972416388931688 26.726416853850584 27.069936506134496 23.231230251083236 32-33 22.89819975661565 27.08070727093429 26.690052243330964 23.3310407291191 34-35 23.26999307233496 26.813105114644408 26.85505972999003 23.0618420830306 36-37 23.394861190653575 26.552649228705565 26.644924120710833 23.407565459930023 38-39 23.089236972054906 26.852796097648007 26.863085185538104 23.194881744758984 40-41 23.139665714568654 26.678229063898723 27.036845888893506 23.145259332639117 42-43 23.172405240207727 26.693879440619178 26.676206175032235 23.457509144140857 44-45 23.085035676052314 26.784064753007392 26.780851456046324 23.35004811489397 46-47 23.18353977764867 27.035839863927354 26.464258375849 23.316361982574985 48-49 23.34130507321977 27.019078955704888 26.20807339294539 23.43154257812995 50-51 23.201967871547012 27.04983662739608 26.153707609417577 23.594487891639336 52-53 23.543100385174704 26.84966900184215 26.203994077666508 23.403236535316633 54-55 23.297702020219084 26.746952573206162 26.258962175210904 23.696383231363846 56-57 23.14583013037653 27.1179470891247 26.27405596544017 23.4621668150586 58-59 23.28211378913169 26.674319494994432 26.275773171925564 23.767793543948315 60-61 23.394785645264484 26.722478020033684 26.41790343584736 23.46483289885447 62-63 23.319990890285382 26.994895721475203 26.327734147711183 23.35737924052823 64-65 23.201589869061856 26.678879360592315 26.36015879768433 23.759371972661498 66-67 23.106275187532173 27.142159855012398 26.071935245540057 23.67962971191537 68-69 22.986035775837816 26.949298612531937 26.453987904193866 23.610677707436384 70-71 22.8811805464866 26.739804333623855 26.77185281620339 23.60716230368615 72-73 23.251963379973322 26.929592673832275 26.17921053947505 23.63923340671935 74-75 23.072728240275772 27.112773076166107 26.17358449766149 23.64091418589663 76-77 23.29356643259763 26.765236633981026 26.446022400411966 23.495174533009376 78-79 23.060221957346258 26.878406724890564 26.486133833405933 23.575237484357242 80-81 23.11611085967079 27.154287440378656 26.23540118640411 23.49420051354644 82-83 23.27714487674536 26.9829778980958 26.296947793661502 23.44292943149734 84-85 23.112210799607787 26.9440125205009 26.176195911240136 23.767580768651175 86-87 23.26863034385891 26.758932497815163 26.41098013059533 23.5614570277306 88-89 23.263075484697385 26.75511999045794 26.326098731317536 23.65570579352714 90-91 23.106878193891244 26.964923116845906 26.564542113749496 23.363656575513353 92-93 23.23434283537371 26.88640715765751 26.253084214145193 23.62616579282359 94-95 23.17942542466544 26.51479788869725 26.40518827832664 23.900588408310668 96-97 23.326673233850244 26.60842922958693 26.273864394472955 23.791033142089873 98-99 23.00971444577382 26.59398374044174 26.302949068003144 24.093352745781292 100-101 22.94018916263814 26.611113410578547 26.588002355030078 23.860695071753238 102-103 23.155717764845484 26.403610786001767 26.610051464884137 23.830619984268612 104-105 23.164856611314182 26.461711771070064 26.63106023899353 23.742371378622224 106-107 22.97791071200809 26.567354547811977 26.69067817011396 23.76405657006598 108-109 23.127241691960798 26.468972896887283 26.33862090088501 24.06516451026691 110-111 22.846534958725755 26.542337965461847 26.67561365693192 23.93551341888048 112-113 22.905558445384845 26.627818125576198 26.75769994967248 23.70892347936648 114-115 22.95853075944921 26.732792695356196 26.4378086406457 23.8708679045489 116-117 22.652833669528547 26.639973780151983 26.82297210086532 23.884220449454148 118-119 22.908298856901226 26.54988519681764 26.40464747204085 24.13716847424028 120-121 22.77287405521731 26.675148832100092 26.61938008672285 23.93259702595975 122-123 22.888607958053868 26.66441524047715 26.605758774269074 23.841218027199908 124-125 23.060615961436362 26.690163544557894 26.18266275900922 24.066557734996525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1354.0 1 1421.0 2 1695.0 3 1731.5 4 1327.0 5 992.5 6 856.5 7 920.0 8 1107.5 9 1261.5 10 1248.5 11 1187.5 12 1241.0 13 1321.0 14 1441.0 15 1607.0 16 1760.0 17 1900.0 18 2121.0 19 2409.0 20 2871.0 21 3514.0 22 4417.5 23 5550.5 24 6960.5 25 8894.5 26 11490.0 27 14775.5 28 17831.0 29 21247.0 30 26639.5 31 32947.5 32 39365.5 33 45765.5 34 52178.5 35 57999.5 36 64217.0 37 70958.5 38 75442.0 39 78433.0 40 80858.0 41 83871.5 42 87055.5 43 92037.5 44 97794.0 45 100766.0 46 101646.5 47 103853.5 48 109246.0 49 112088.0 50 111042.0 51 109237.0 52 109142.0 53 110674.0 54 109552.5 55 103739.5 56 96479.5 57 90362.5 58 84998.5 59 77989.5 60 67646.0 61 55725.0 62 44574.0 63 34671.0 64 26709.5 65 20765.0 66 15867.0 67 12391.0 68 9558.5 69 7036.0 70 5358.5 71 4132.0 72 3192.0 73 2619.5 74 2084.5 75 1575.5 76 1143.5 77 772.0 78 515.0 79 341.0 80 228.5 81 166.5 82 116.0 83 74.5 84 56.5 85 50.5 86 40.0 87 25.0 88 19.5 89 23.0 90 15.5 91 9.0 92 10.0 93 8.0 94 7.5 95 5.0 96 4.0 97 5.0 98 5.0 99 4.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09494157376639305 2 0.03230040730273016 3 0.04382178689502199 4 0.05084949057595073 5 0.05385653686250197 6 0.003378703692754201 7 0.002500240732638109 8 0.002398879621855483 9 0.0070277036809287385 10-11 0.059431397955546396 12-13 0.00655468516394315 14-15 0.012163333293915123 16-17 0.021674384189018202 18-19 0.01981609715800339 20-21 0.13339122178993584 22-23 0.01743411105461168 24-25 0.015981268466727372 26-27 0.007466935160986785 28-29 0.004645717577537027 30-31 0.0019934351787249784 32-33 0.0067067268301170884 34-35 0.019089675864061236 36-37 0.0027029629542033606 38-39 0.0090887129335088 40-41 0.03331401841055642 42-43 0.014967657358901109 44-45 0.10980787001451152 46-47 0.00403755091284127 48-49 0.010220578670581458 50-51 0.012687032366292025 52-53 0.013802004584900912 54-55 0.01125108329687149 56-57 0.007095277754783822 58-59 0.015558930505133095 60-61 0.033719462853686925 62-63 0.008007527751827457 64-65 0.007635870345624495 66-67 0.009781347190523412 68-69 0.008767736082697151 70-71 0.0046119305406094845 72-73 7.771018493334662E-4 74-75 0.0010980787001451152 76-77 0.008801523119624694 78-79 0.022907611036873485 80-81 0.00336181017429043 82-83 0.0051018425760588435 84-85 0.003007046286551239 86-87 0.00386861572820356 88-89 0.006351962942377898 90-91 0.00537213887147918 92-93 0.002956365731159926 94-95 0.004071337949768813 96-97 0.0016724583279133292 98-99 0.003446277766609285 100-101 0.0031253009157976358 102-103 9.122499970436342E-4 104-105 0.004628824059073255 106-107 7.602083308696952E-4 108-109 0.0012332268478552834 110-111 0.0020441157341162916 112-113 0.0034293842481455143 114-115 0.0021454768448989176 116-117 0.004223379615942751 118-119 0.0028550046203773 120-121 0.005811370351537226 122-123 0.0036996805435658498 124-125 0.05377206927018311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2959715.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.82994844085788 #Duplication Level Percentage of deduplicated Percentage of total 1 80.7414614967683 45.88533093900854 2 9.714969012221397 11.04202376138148 3 3.5950001851314797 6.129110254976896 4 1.7816339999305486 4.050006734261298 5 1.0445945974039486 2.9682128556032548 6 0.6515925462340242 2.221798248415614 7 0.44171508986603164 1.7571852047844838 8 0.3203708163884467 1.456532558184878 9 0.23553844522101597 1.204707392797515 >10 1.319275036336537 13.732957671322943 >50 0.10336342496388277 4.062010566110063 >100 0.04866046220036737 4.705409951872698 >500 0.0015731787362277673 0.5346696869361965 >1k 2.517085977623536E-4 0.2500441743440568 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.378703692754201E-5 2 3.378703692754201E-5 0.0 0.0 0.0 3.378703692754201E-5 3 3.378703692754201E-5 0.0 0.0 0.0 3.378703692754201E-5 4 3.378703692754201E-5 0.0 0.0 0.0 3.378703692754201E-5 5 6.757407385508402E-5 0.0 0.0 0.0 3.378703692754201E-5 6 6.757407385508402E-5 0.0 0.0 0.0 3.378703692754201E-5 7 1.0136111078262603E-4 0.0 0.0 0.0 3.378703692754201E-5 8 1.0136111078262603E-4 0.0 0.0 0.0 3.378703692754201E-5 9 1.0136111078262603E-4 0.0 0.0 0.0 3.378703692754201E-5 10-11 1.0136111078262603E-4 0.0 0.0 1.6893518463771006E-5 3.378703692754201E-5 12-13 1.0136111078262603E-4 0.0 0.0 3.378703692754201E-5 3.378703692754201E-5 14-15 1.3514814771016805E-4 0.0 0.0 3.378703692754201E-5 3.378703692754201E-5 16-17 1.3514814771016805E-4 0.0 0.0 3.378703692754201E-5 3.378703692754201E-5 18-19 1.8582870310148106E-4 0.0 0.0 3.378703692754201E-5 3.378703692754201E-5 20-21 2.3650925849279407E-4 0.0 0.0 6.757407385508402E-5 3.378703692754201E-5 22-23 2.702962954203361E-4 0.0 0.0 2.0272222156525205E-4 3.378703692754201E-5 24-25 3.716574062029621E-4 0.0 0.0 6.250601831595272E-4 3.378703692754201E-5 26-27 3.716574062029621E-4 0.0 0.0 0.0010136111078262603 3.378703692754201E-5 28-29 4.561249985218171E-4 0.0 0.0 0.0016555648094495586 3.378703692754201E-5 30-31 5.068055539131301E-4 0.0 0.0 0.003767254617420934 5.0680555391313014E-5 32-33 5.236990723769012E-4 3.378703692754201E-5 0.0 0.008058208307218769 6.757407385508402E-5 34-35 6.250601831595271E-4 3.378703692754201E-5 0.0 0.014275023101886499 6.757407385508402E-5 36-37 6.757407385508401E-4 6.757407385508402E-5 0.0 0.024647643438641897 6.757407385508402E-5 38-39 6.757407385508401E-4 6.757407385508402E-5 0.0 0.04333187485957263 6.757407385508402E-5 40-41 6.757407385508401E-4 1.0136111078262603E-4 0.0 0.06546238404711265 6.757407385508402E-5 42-43 7.771018493334663E-4 1.0136111078262603E-4 0.0 0.08592043490673934 6.757407385508402E-5 44-45 8.784629601160923E-4 1.0136111078262603E-4 0.0 0.11072012001155516 6.757407385508402E-5 46-47 9.967175893624892E-4 1.0136111078262603E-4 0.0 0.14349354583127094 6.757407385508402E-5 48-49 0.0010136111078262603 1.0136111078262603E-4 0.0 0.18052413830385697 6.757407385508402E-5 50-51 0.0010136111078262603 1.0136111078262603E-4 0.0 0.22184568446624084 6.757407385508402E-5 52-53 0.0010136111078262603 1.0136111078262603E-4 0.0 0.2612075824868273 6.757407385508402E-5 54-55 0.0010136111078262603 1.0136111078262603E-4 0.0 0.3052320916034145 6.757407385508402E-5 56-57 0.0010473981447538022 1.0136111078262603E-4 0.0 0.3575682118041771 6.757407385508402E-5 58-59 0.0010473981447538022 1.0136111078262603E-4 0.0 0.4200742301201298 6.757407385508402E-5 60-61 0.0010473981447538022 1.0136111078262603E-4 0.0 0.4889997854523155 6.757407385508402E-5 62-63 0.0010473981447538022 1.3514814771016805E-4 0.0 0.5621825074373715 6.757407385508402E-5 64-65 0.0010473981447538022 1.3514814771016805E-4 0.0 0.6360578636794421 1.0136111078262603E-4 66-67 0.0011149722186088863 1.3514814771016805E-4 0.0 0.7228229745093699 1.0136111078262603E-4 68-69 0.0011487592555364282 1.3514814771016805E-4 0.0 0.8286777612033591 1.0136111078262603E-4 70-71 0.0012839074032465962 1.3514814771016805E-4 0.0 0.9509361543256699 1.0136111078262603E-4 72-73 0.0013514814771016805 1.3514814771016805E-4 0.0 1.0755765335513723 1.0136111078262603E-4 74-75 0.0013852685140292224 1.3514814771016805E-4 0.0 1.2003013803693936 1.0136111078262603E-4 76-77 0.0013852685140292224 1.3514814771016805E-4 0.0 1.3426968475005197 1.0136111078262603E-4 78-79 0.0013852685140292224 1.3514814771016805E-4 0.0 1.5083715830747217 1.0136111078262603E-4 80-81 0.0016555648094495586 1.3514814771016805E-4 0.0 1.6931697815499127 1.0136111078262603E-4 82-83 0.0016555648094495586 1.3514814771016805E-4 0.0 1.8930538920132514 1.0136111078262603E-4 84-85 0.0017569259202321846 1.3514814771016805E-4 0.0 2.093461701548967 1.0136111078262603E-4 86-87 0.0018244999940872685 1.3514814771016805E-4 0.0 2.302451418464278 1.0136111078262603E-4 88-89 0.0018582870310148106 1.3514814771016805E-4 0.0 2.538538338995478 1.0136111078262603E-4 90-91 0.0019596481417974367 1.3514814771016805E-4 0.0 2.8086488057127124 1.0136111078262603E-4 92-93 0.0020272222156525205 1.3514814771016805E-4 0.0 3.1054003510473134 1.0136111078262603E-4 94-95 0.0020272222156525205 1.3514814771016805E-4 0.0 3.405767109333162 1.0136111078262603E-4 96-97 0.0020272222156525205 1.3514814771016805E-4 0.0 3.7085158537224023 1.0136111078262603E-4 98-99 0.0022130509187540015 1.3514814771016805E-4 0.0 4.033986380445414 1.0136111078262603E-4 100-101 0.0023313055480003986 1.3514814771016805E-4 0.0 4.4074007125686085 1.0136111078262603E-4 102-103 0.002381986103391712 1.3514814771016805E-4 0.0 4.811138910334272 1.0136111078262603E-4 104-105 0.0028043240649859866 1.3514814771016805E-4 0.0 5.216313057169356 1.0136111078262603E-4 106-107 0.002905685175768613 1.3514814771016805E-4 0.0 5.625288245658789 1.0136111078262603E-4 108-109 0.002973259249623697 1.3514814771016805E-4 0.0 6.053995063713905 1.0136111078262603E-4 110-111 0.003074620360406323 1.3514814771016805E-4 0.0 6.5338216686403925 1.0136111078262603E-4 112-113 0.003091513878870094 1.3514814771016805E-4 0.0 7.048060370677582 1.0136111078262603E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5725 0.0 47.928116 1 GGTATCA 3335 0.0 46.759556 1 ATCAACG 6810 0.0 38.268173 3 GTACATG 9830 0.0 37.84351 1 CAACGCA 7025 0.0 37.314304 5 TCAACGC 7040 0.0 36.896927 4 AACGCAG 7290 0.0 35.123425 6 TACATGG 10205 0.0 34.631355 2 TATCAAC 7910 0.0 34.07358 2 ACATGGG 10285 0.0 31.759876 3 ACGCAGA 8515 0.0 29.581417 7 CGCAGAG 8520 0.0 28.587029 8 GCAGAGT 9525 0.0 23.507593 9 CATGGGG 5780 0.0 23.50084 4 CATGGGA 6180 0.0 23.329384 4 GTATAGG 970 0.0 22.09 1 GAGTACA 8090 0.0 21.556786 1 AGTACAT 7435 0.0 21.28613 2 AGAGTAC 8925 0.0 20.8392 10-11 CAGAGTA 9150 0.0 19.87144 10-11 >>END_MODULE