FastQCFastQC Report
Thu 26 May 2016
SRR1364517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959715
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66600.2250216659374298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63870.2157978048562108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41080.1387971476983426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46300.063.9133571
GTATCAA83400.049.789081
ATCAACG105050.036.700293
CAACGCA107000.035.696215
TCAACGC107900.035.3995634
TATCAAC116950.034.8511542
AACGCAG109100.034.518336
GTACATG101000.033.67011
TACATGG101950.031.5622432
ACATGGG100900.029.1740743
ACGCAGA132850.028.2130367
CGCAGAG135300.028.1748948
GTGGTAT26600.026.0176031
TGGTATC25250.025.487292
GCAGAGT147300.024.6289
GAGTACT91850.024.37866212-13
CATGGGG56600.023.0562674
AGAGTAC136650.023.04519710-11
CAGAGTA142800.022.8023710-11
GTACTTT100200.022.61420414-15
Produced by FastQC (version 0.11.5)