FastQCFastQC Report
Thu 26 May 2016
SRR1364516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448883
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63250.052.9921651
GGTATCA35000.051.8794751
ATCAACG74200.042.4998323
TCAACGC74850.042.033614
CAACGCA75000.041.5505945
AACGCAG77000.039.9866036
TATCAAC82750.039.833472
GTACATG110900.036.1282841
TACATGG111600.034.1210332
ACGCAGA91600.032.8993647
CGCAGAG91150.032.083668
ACATGGG111250.031.287523
GCAGAGT100150.027.7731469
ACGCATA2705.820766E-1126.471388
CATGGGA61300.026.1484784
CATGGGG55950.023.962774
AGAGTAC98900.022.99016610-11
CAGAGTA104700.021.83029210-11
CGCATAG2755.4624252E-821.6584039
GAGTACT48300.021.18226412-13
Produced by FastQC (version 0.11.5)