##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364516_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2448883 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.823503205338923 31.0 26.0 31.0 10.0 34.0 2 27.078335714691146 31.0 27.0 31.0 10.0 34.0 3 27.12038713160245 31.0 27.0 31.0 10.0 34.0 4 29.98007132231307 35.0 28.0 37.0 10.0 37.0 5 30.701201323215525 35.0 30.0 37.0 12.0 37.0 6 30.840761277692728 35.0 31.0 37.0 12.0 37.0 7 30.638671181922533 35.0 30.0 37.0 10.0 37.0 8 30.25340083621798 35.0 30.0 37.0 10.0 37.0 9 30.965122057689157 37.0 29.0 39.0 10.0 39.0 10-11 31.061357974227434 36.0 29.5 39.0 8.0 39.0 12-13 30.910070836377237 37.0 29.5 39.0 8.0 39.0 14-15 31.638277941412472 37.0 29.5 40.0 8.0 41.0 16-17 31.921950946615254 37.0 30.0 40.0 8.0 41.0 18-19 31.9010934781286 37.0 30.0 40.0 8.0 41.0 20-21 31.688220302889114 37.0 29.5 40.0 4.5 41.0 22-23 31.750140778469202 37.0 30.0 40.0 2.0 41.0 24-25 31.777975713825448 37.0 30.0 40.0 2.0 41.0 26-27 31.90877473525685 37.0 30.0 40.0 2.0 41.0 28-29 31.86242931981642 37.0 30.0 40.0 2.0 41.0 30-31 31.53621671594764 37.0 30.0 40.0 2.0 41.0 32-33 31.539011459510316 37.0 30.0 40.0 2.0 41.0 34-35 31.25929148105483 37.0 30.0 40.0 2.0 41.0 36-37 31.04270906368332 37.0 29.0 40.0 2.0 41.0 38-39 30.897874255323753 36.0 29.0 40.0 2.0 41.0 40-41 30.918652095669742 36.5 29.0 40.0 2.0 41.0 42-43 30.795445515363532 36.0 29.0 40.0 2.0 41.0 44-45 30.447620609069524 36.0 28.0 40.0 2.0 41.0 46-47 30.210857970756464 36.0 27.5 40.0 2.0 41.0 48-49 29.964241043773836 35.5 27.0 40.0 2.0 41.0 50-51 29.96294269673153 36.0 27.5 40.0 2.0 41.0 52-53 29.771307571656138 35.0 27.0 40.0 2.0 41.0 54-55 29.658669483188866 35.0 26.5 40.0 2.0 41.0 56-57 29.23791500043081 35.0 26.0 39.0 2.0 41.0 58-59 29.022467794500592 35.0 26.0 39.0 2.0 41.0 60-61 28.59790606574508 34.0 24.5 39.0 2.0 40.5 62-63 28.566425386594624 34.0 25.5 39.0 2.0 40.0 64-65 28.387466653163912 34.0 25.5 38.5 2.0 40.0 66-67 28.129803669673073 34.0 25.0 38.0 2.0 40.0 68-69 27.955653455065026 34.0 25.0 37.5 2.0 40.0 70-71 27.796532541570993 34.0 24.5 37.0 2.0 40.0 72-73 27.774668287541708 34.0 25.5 37.0 2.0 39.5 74-75 27.494295970856918 34.0 25.0 36.0 2.0 39.0 76-77 27.18289277192908 33.0 24.5 36.0 2.0 39.0 78-79 26.839771030302387 33.0 23.5 35.5 2.0 38.5 80-81 26.84908425596486 33.0 25.0 35.0 2.0 37.0 82-83 26.643099935766635 33.0 24.5 35.0 2.0 37.0 84-85 26.454995808293006 33.0 24.0 35.0 2.0 36.5 86-87 26.248228682219604 33.0 24.0 35.0 2.0 36.0 88-89 26.011544855348337 33.0 23.0 35.0 2.0 36.0 90-91 25.835307566755944 33.0 22.0 35.0 2.0 35.5 92-93 25.740340596100346 33.0 22.5 35.0 2.0 35.0 94-95 25.67666870977503 33.0 22.0 35.0 2.0 35.0 96-97 25.53871908131177 33.0 20.0 35.0 2.0 35.0 98-99 25.45431794822374 33.0 20.0 35.0 2.0 35.0 100-101 25.41203724310226 33.0 20.0 35.0 2.0 35.0 102-103 24.235852223238105 30.5 17.5 34.0 2.0 35.0 104-105 25.034995342774643 32.0 18.0 35.0 2.0 35.0 106-107 25.137304436349144 33.0 18.0 35.0 2.0 35.0 108-109 25.098941639923183 33.0 17.5 35.0 2.0 35.0 110-111 24.99785187777448 33.0 17.0 35.0 2.0 35.0 112-113 25.004061443523433 33.0 16.5 35.0 2.0 35.0 114-115 24.968371702527236 33.0 13.5 35.0 2.0 35.0 116-117 24.769605162843632 33.0 7.5 35.0 2.0 35.0 118-119 24.64400361307584 33.0 2.0 35.0 2.0 35.0 120-121 24.52337024676148 33.0 2.0 35.0 2.0 35.0 122-123 24.263283913523022 33.0 2.0 35.0 2.0 35.0 124-125 23.278706046797666 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.8853485634026725 1101 2 1.3716456794900402 1101 3 1.7666468791014367 1101 4 2.073963966997532 1101 5 1.5747728760713962 1101 6 1.3997901197939484 1101 7 1.3292759191339591 1101 8 1.7512539171786052 1101 9 2.309405169886599 1101 10-11 2.2454474026388453 1101 12-13 2.135855628663773 1101 14-15 2.4119477592042706 1101 16-17 2.118321808508501 1101 18-19 2.0727483963084197 1101 20-21 2.4180905238420998 1101 22-23 2.351680889654606 1101 24-25 2.229251880368487 1101 26-27 1.8778285167119755 1101 28-29 1.7918138306834592 1101 30-31 1.8757691345264753 1101 32-33 2.0912714701340285 1101 34-35 2.389743899690739 1101 36-37 2.4113426546246366 1101 38-39 2.3710456543196052 1101 40-41 2.19147612793817 1101 42-43 2.2163610433802248 1101 44-45 2.3972393381137387 1101 46-47 2.415784526350887 1101 48-49 2.4703848786969544 1101 50-51 2.335741045384353 1101 52-53 2.1662688572210875 1101 54-55 2.264088498592713 1101 56-57 2.525699967963419 1101 58-59 2.4301293349444073 1101 60-61 2.6010634922798417 1101 62-63 2.298395499521053 1101 64-65 2.3540582151429064 1101 66-67 2.4384944286329038 1101 68-69 2.174026409045993 1101 70-71 2.249543915412108 1101 72-73 2.080149198061008 1101 74-75 2.0123215226568014 1101 76-77 2.232200385615638 1101 78-79 2.240093436473021 1101 80-81 2.0336609234049092 1101 82-83 2.004935317293672 1101 84-85 1.8789973600282615 1101 86-87 1.8415428871286004 1101 88-89 1.9004614793852284 1101 90-91 1.965529781946028 1101 92-93 2.003574378227622 1101 94-95 1.9393040172545994 1101 96-97 1.9389206295376233 1101 98-99 1.9151820336228624 1101 100-101 1.8841973155990281 1101 102-103 1.9378275502504607 1101 104-105 1.9363483154816343 1101 106-107 2.1097035467638534 1101 108-109 2.1782585030527493 1101 110-111 2.2596675711763083 1101 112-113 2.1389480865648665 1101 114-115 2.067288007346047 1101 116-117 2.1350381537132 1101 118-119 2.1905441180789538 1101 120-121 2.0854033703721413 1101 122-123 2.178829426333685 1101 124-125 2.1965557101269013 1102 1 1.0555824870045143 1102 2 0.7707246446865561 1102 3 0.8784245999902893 1102 4 1.4331985081195135 1102 5 1.2063248519064445 1102 6 1.1372831960137972 1102 7 1.1094403556628905 1102 8 1.3280064096629935 1102 9 1.7408147093034323 1102 10-11 1.467753123126414 1102 12-13 1.7131276913981885 1102 14-15 1.985458806208289 1102 16-17 1.4160984530955396 1102 18-19 1.5854742354124625 1102 20-21 1.7340769013906758 1102 22-23 1.6185652788127989 1102 24-25 1.4793046784932358 1102 26-27 1.4708933740660477 1102 28-29 1.1931093613241046 1102 30-31 1.0945577582089676 1102 32-33 1.1006968338484917 1102 34-35 1.4408657126491171 1102 36-37 1.7013746057099475 1102 38-39 1.5963824306565009 1102 40-41 1.310927392936886 1102 42-43 1.3454523436867802 1102 44-45 1.7754621647043543 1102 46-47 1.6561264456303881 1102 48-49 1.7027331007191435 1102 50-51 1.5003527524528621 1102 52-53 1.5682358530788996 1102 54-55 1.4214954217305689 1102 56-57 1.6388157638471696 1102 58-59 1.5902752229301065 1102 60-61 1.941240663058874 1102 62-63 1.805081363142385 1102 64-65 1.8885363846284804 1102 66-67 1.8474187721698456 1102 68-69 1.6680418314508714 1102 70-71 1.87840444853477 1102 72-73 1.7572615588934113 1102 74-75 1.9031217724787162 1102 76-77 1.8134338752100128 1102 78-79 1.6984893170417514 1102 80-81 1.3574680757500914 1102 82-83 1.2471648555543808 1102 84-85 1.3582274341459737 1102 86-87 1.5213564015522394 1102 88-89 1.2346873342407747 1102 90-91 1.0931819605931672 1102 92-93 1.1615407091578582 1102 94-95 1.3400380755853263 1102 96-97 1.455294547615729 1102 98-99 1.6059523250012262 1102 100-101 1.4370151679563534 1102 102-103 1.2653787434039714 1102 104-105 1.3008312043013994 1102 106-107 1.5624071318979809 1102 108-109 1.6773167389637926 1102 110-111 1.3678139586223281 1102 112-113 1.3085331781789833 1102 114-115 1.4103117272763477 1102 116-117 1.7206281576556677 1102 118-119 1.7621421219504576 1102 120-121 1.696683580763299 1102 122-123 1.912788277899157 1102 124-125 1.8075234901955284 1103 1 1.335240862389032 1103 2 1.119473839436914 1103 3 1.2763104155715759 1103 4 1.7727316639952058 1103 5 1.218740185032484 1103 6 1.2563617505298552 1103 7 1.327203391758868 1103 8 1.597522237868585 1103 9 2.1749657932138184 1103 10-11 1.7296295225320648 1103 12-13 1.8971385919976207 1103 14-15 2.1365956446917487 1103 16-17 1.9870401186370081 1103 18-19 1.7510357328771278 1103 20-21 2.093345346700449 1103 22-23 1.8650967937650655 1103 24-25 1.8992344387152258 1103 26-27 1.7180139902009195 1103 28-29 1.523098171790565 1103 30-31 1.7678992676011127 1103 32-33 1.890782425821886 1103 34-35 1.7592802898552584 1103 36-37 1.9670904247616399 1103 38-39 2.039505056079161 1103 40-41 1.974858552593087 1103 42-43 1.9841512689264889 1103 44-45 1.8800475964084065 1103 46-47 2.020841801732555 1103 48-49 2.1215967976091292 1103 50-51 2.0010605801229246 1103 52-53 2.025864852377584 1103 54-55 1.895721911810817 1103 56-57 2.125751623594862 1103 58-59 2.2514698474760593 1103 60-61 2.2462420030156096 1103 62-63 2.081240505679741 1103 64-65 1.9017740452795415 1103 66-67 1.964288777937103 1103 68-69 1.8669448718161235 1103 70-71 2.0667866390742553 1103 72-73 1.9481154917292507 1103 74-75 1.970055828863213 1103 76-77 2.0004534908210125 1103 78-79 2.146282180978229 1103 80-81 1.653230454014512 1103 82-83 1.81281591955009 1103 84-85 1.7414550597814795 1103 86-87 1.742058965351351 1103 88-89 1.7858510632455484 1103 90-91 1.7505967191256495 1103 92-93 1.7016373246457164 1103 94-95 1.6010418848093053 1103 96-97 1.7606102576431546 1103 98-99 1.7456524671303093 1103 100-101 1.5300491749844447 1103 102-103 1.636703143844727 1103 104-105 1.8738096878481123 1103 106-107 1.6030363411574875 1103 108-109 1.8636082314532167 1103 110-111 1.9522074722721818 1103 112-113 1.9205559218788046 1103 114-115 1.9226752746349263 1103 116-117 2.0843421826351403 1103 118-119 2.081433820592931 1103 120-121 2.1083171087273485 1103 122-123 2.109325546402495 1103 124-125 1.9857973654714876 1104 1 1.3964192718347093 1104 2 1.2069765408590314 1104 3 1.344919510505175 1104 4 1.5530609800790174 1104 5 1.1772934503365846 1104 6 1.0763449318019838 1104 7 1.0599716169829705 1104 8 1.448423598639618 1104 9 1.940484074043841 1104 10-11 1.8264881791074998 1104 12-13 1.3416973434652029 1104 14-15 1.6271343573793295 1104 16-17 1.3433717143123793 1104 18-19 0.7011880235325094 1104 20-21 -3.626597128603855 1104 22-23 -0.320894272863228 1104 24-25 1.033195864343341 1104 26-27 1.084607297213406 1104 28-29 0.7987051080243788 1104 30-31 1.6213683238235355 1104 32-33 1.4111061906356106 1104 34-35 1.5374621994261979 1104 36-37 1.7096227762185556 1104 38-39 1.7387734600596083 1104 40-41 1.75460560196613 1104 42-43 1.8043634347119948 1104 44-45 1.8176934319735132 1104 46-47 1.7564033727176103 1104 48-49 1.7613984935070839 1104 50-51 1.7143478412128808 1104 52-53 1.8288864727891614 1104 54-55 1.6108353485948115 1104 56-57 1.8527280499851813 1104 58-59 1.970934240975783 1104 60-61 1.9176942918497275 1104 62-63 1.6400526342655688 1104 64-65 1.6843936725686177 1104 66-67 1.7205949763008626 1104 68-69 1.6708843304188647 1104 70-71 1.8757246786685364 1104 72-73 1.7169291131779474 1104 74-75 1.7736848121432907 1104 76-77 1.7316725302290088 1104 78-79 1.7520465676374535 1104 80-81 1.4606938433811116 1104 82-83 1.393680651403379 1104 84-85 1.3328475222437248 1104 86-87 1.2432978551030693 1104 88-89 1.4038714453410037 1104 90-91 1.4065523815286944 1104 92-93 1.1470119522008098 1104 94-95 1.3524861328344855 1104 96-97 1.424331173168195 1104 98-99 1.3519496810696054 1104 100-101 0.8688697832034507 1104 102-103 1.3049965434548625 1104 104-105 1.5408315009981841 1104 106-107 1.713089625319565 1104 108-109 1.658693944870297 1104 110-111 1.6774178820436774 1104 112-113 1.6496116724912575 1104 114-115 1.5417150985086607 1104 116-117 1.6385501342738387 1104 118-119 1.6458961339796971 1104 120-121 1.6309666061803725 1104 122-123 1.4799062743602889 1104 124-125 1.6110898611897255 1105 1 1.278899929822444 1105 2 1.0344369192542686 1105 3 1.1332304673999154 1105 4 1.5524735012361859 1105 5 1.2866668810058677 1105 6 1.1165052969532283 1105 7 1.1370403547220995 1105 8 1.4259671770115396 1105 9 1.8925821178621582 1105 10-11 1.5890686842107158 1105 12-13 1.7870809912944985 1105 14-15 1.9497777164324752 1105 16-17 1.6476485374291023 1105 18-19 1.3521373690550718 1105 20-21 1.3844046342612835 1105 22-23 1.5050298312136796 1105 24-25 1.4381807337628771 1105 26-27 1.4109799753897256 1105 28-29 1.0093337032427456 1105 30-31 1.4111649792801977 1105 32-33 1.4229153797362208 1105 34-35 1.2568250777639314 1105 36-37 1.5066404781619092 1105 38-39 1.3355101308118584 1105 40-41 1.3724403638264597 1105 42-43 1.6714231659661394 1105 44-45 1.8746072664573497 1105 46-47 2.021746864253526 1105 48-49 1.776635833102283 1105 50-51 1.768673954261697 1105 52-53 1.5793274748120503 1105 54-55 1.623379636313146 1105 56-57 1.9989008796316021 1105 58-59 1.7301680668417383 1105 60-61 2.1332548805343876 1105 62-63 1.9917400143713841 1105 64-65 1.7378283521933469 1105 66-67 1.9385557703745278 1105 68-69 1.5594126844880236 1105 70-71 1.5754246601383777 1105 72-73 1.524038535827117 1105 74-75 1.8083825312766848 1105 76-77 1.6939073343215298 1105 78-79 1.6040570468198858 1105 80-81 1.5376135326516618 1105 82-83 1.5554293853307541 1105 84-85 1.5994321199828718 1105 86-87 1.5673660860845473 1105 88-89 1.6129555869696937 1105 90-91 1.5598536068764055 1105 92-93 1.5687561128797043 1105 94-95 1.494559611693603 1105 96-97 1.5947562209687156 1105 98-99 1.432743380416163 1105 100-101 1.479562362468446 1105 102-103 1.464872438549314 1105 104-105 1.5503148124986943 1105 106-107 1.7130424427106483 1105 108-109 1.7482282493057788 1105 110-111 1.6179755811953633 1105 112-113 1.5968261198418254 1105 114-115 1.5952087474651897 1105 116-117 1.7258865720633025 1105 118-119 1.8535035063819905 1105 120-121 1.8133389263260575 1105 122-123 1.8258702718509277 1105 124-125 1.6405580971144857 1106 1 -0.529456744316704 1106 2 -0.29892598829217576 1106 3 -0.3166686284279514 1106 4 -0.6260087767732081 1106 5 -1.0532020205964479 1106 6 -0.5236158508153288 1106 7 -0.48285103279157227 1106 8 -0.20102209296945261 1106 9 0.044696869042226695 1106 10-11 -2.4494559125006674 1106 12-13 -0.5423464895017567 1106 14-15 -0.29867733998770163 1106 16-17 -0.5380395308879038 1106 18-19 -3.0289732600882857 1106 20-21 -5.469145740513419 1106 22-23 -6.254126038487858 1106 24-25 -6.086283250265904 1106 26-27 -5.140455726657404 1106 28-29 -1.842662884216388 1106 30-31 -3.7642429237673554 1106 32-33 -4.295606840577783 1106 34-35 -3.5466723201807824 1106 36-37 -5.757320214570765 1106 38-39 -6.2827714828332795 1106 40-41 -2.6658543314155523 1106 42-43 -1.3010829433435767 1106 44-45 -0.5461457648044039 1106 46-47 -0.3299218987588901 1106 48-49 -0.16916155234708796 1106 50-51 -0.42362598331864376 1106 52-53 -0.5439187909918495 1106 54-55 -0.3576486605031626 1106 56-57 -0.2215430701976011 1106 58-59 -0.15140172335631874 1106 60-61 0.16404292015203836 1106 62-63 -0.15947752555237926 1106 64-65 -0.26153479185171236 1106 66-67 -0.4355030136517435 1106 68-69 -0.37196934036230545 1106 70-71 -0.10002671272322061 1106 72-73 -0.1609880709798226 1106 74-75 -0.2372720777645796 1106 76-77 -0.2372545243007984 1106 78-79 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82-83 -4.343775819578603 2216 84-85 -4.082269835237632 2216 86-87 -4.297744990139279 2216 88-89 -4.401450388955421 2216 90-91 -4.512467584965989 2216 92-93 -4.387336266457837 2216 94-95 -7.677900692331647 2216 96-97 -9.819665454233219 2216 98-99 -6.388424529033109 2216 100-101 -5.368949447473835 2216 102-103 -6.562558960065768 2216 104-105 -9.421138986618764 2216 106-107 -5.724507523847738 2216 108-109 -8.26600626348053 2216 110-111 -5.5253311964988825 2216 112-113 -4.871995607957295 2216 114-115 -5.281198834025886 2216 116-117 -4.831628389101727 2216 118-119 -5.2375126976680555 2216 120-121 -5.9494121276914385 2216 122-123 -9.049148000448263 2216 124-125 -7.546998766680002 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 190841.0 3 29341.0 4 17011.0 5 13797.0 6 14483.0 7 15982.0 8 17839.0 9 19285.0 10 20599.0 11 21370.0 12 21858.0 13 22730.0 14 23878.0 15 20488.0 16 16250.0 17 14794.0 18 13475.0 19 12755.0 20 12867.0 21 14082.0 22 17082.0 23 22785.0 24 30527.0 25 39251.0 26 48183.0 27 55231.0 28 63634.0 29 73168.0 30 83020.0 31 94333.0 32 113660.0 33 139648.0 34 175625.0 35 224650.0 36 299100.0 37 331820.0 38 102981.0 39 460.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.036759121724636 19.089957090105873 13.650521025357632 23.22276276281186 2 15.035617606727211 20.046959115423594 41.07443893946579 23.8429843383834 3 18.743409982143376 25.84504404103061 28.64932260437914 26.762223372446876 4 11.093765576253986 16.228148303097356 38.69215030339478 33.98593581725387 5 13.309189678587597 38.64625841446136 34.19474483283842 13.84980707411263 6 31.50065726418835 37.05365146090697 17.777577224968933 13.668114049935745 7 28.744380463594144 32.26488807071806 21.204921711369096 17.785809754318706 8 25.602159086871882 36.003892498120514 20.074542237402365 18.31940617760523 9 26.018475033793305 16.526906003569223 20.623797836411825 36.83082112622564 10-11 24.50976546298759 27.12842226387447 27.67834632139706 20.683465951740875 12-13 25.2383083719726 24.461636660489287 28.176846006767626 22.123208960770494 14-15 22.554879296381895 25.84604433307019 25.67239415486639 25.926682215681524 16-17 22.302695558375582 29.15681143426153 26.064211936066616 22.476281071296267 18-19 22.32743832707074 28.00871998039536 27.936897565757228 21.726944126776672 20-21 23.186047833406185 27.122995339000898 28.01741322114333 21.673543606449588 22-23 22.78716370753256 27.005216331545 27.80034748315679 22.407272477765652 24-25 22.461126152084322 27.2108154512748 27.969219484442903 22.358838912197974 26-27 22.35109121300022 27.43869494674106 27.742591263843586 22.46762257641513 28-29 22.230398682764037 27.45966944956721 27.842454397402115 22.467477470266633 30-31 22.437563065805683 27.30496714554822 27.87195196401875 22.385517824627343 32-33 22.416684124745835 27.306110164359136 27.754895102467696 22.522310608427333 34-35 22.64181999114126 27.215037231008694 27.826490409464515 22.31665236838553 36-37 22.682868015200043 27.024040467027127 27.747514760749976 22.545576757022854 38-39 22.417705876203108 27.37999947726426 27.814237687939837 22.388056958592795 40-41 22.575914066075775 27.224187113873597 27.782229051063194 22.417669768987427 42-43 22.367709557715415 27.218570120489403 27.627001520105665 22.786718801689513 44-45 22.50233077433628 27.334540573257037 27.624167774052765 22.53896087835392 46-47 22.669164788005098 27.54625938250312 27.256338734472585 22.5282370950192 48-49 22.626164777797335 27.479689771028283 27.230388423302216 22.663757027872173 50-51 22.581981674396268 27.55695508773191 27.117213292842102 22.743849945029723 52-53 22.755026465506624 27.585918198193266 27.027264342025703 22.6317909942744 54-55 22.646551935375843 27.43573931438933 27.16040055214774 22.757308198087088 56-57 22.426531319405065 27.611131314463698 27.23238141170864 22.7299559544226 58-59 22.658027880017578 27.275461794443274 27.179851925184973 22.886658400354175 60-61 22.795878968704606 27.387186747863996 27.242948201572858 22.57398608185854 62-63 22.571608726138763 27.52037927233716 27.219238539479697 22.688773462044384 64-65 22.47965397100049 27.344956796401675 27.19965500011945 22.97573423247838 66-67 22.377225556703124 27.58316089186848 27.074433046359697 22.965180505068698 68-69 22.48172627836416 27.41245081761849 27.23858067237821 22.86724223163914 70-71 22.27838884531818 27.30445197794562 27.558355424396495 22.8588037523397 72-73 22.602963620864788 27.438453567727873 27.018789335865122 22.939793475542217 74-75 22.397390290031524 27.597780921651328 27.07435251869803 22.93047626961912 76-77 22.5860681161332 27.361536114896612 27.244390936961317 22.808004832008873 78-79 22.50610689938203 27.386929182840596 27.241008955151948 22.86595496262543 80-81 22.458952829316093 27.542445815774553 27.094613950474077 22.903987404435277 82-83 22.634746286844422 27.606018475885442 26.99323882153392 22.76599641573621 84-85 22.63202621024962 27.468961492772216 26.875204435781747 23.023807861196424 86-87 22.485084953337072 27.466513502305528 27.1574623420467 22.890939202310697 88-89 22.619486864933947 27.333641928484198 27.09639664454841 22.950474562033445 90-91 22.53027312942637 27.530707024713653 27.180875120852598 22.758144725007377 92-93 22.595805952620136 27.47671924743671 27.050286588881754 22.8771882110614 94-95 22.49725372329782 27.253866226717903 27.18040077263279 23.068479277351486 96-97 22.581836639521864 27.215087784348686 27.13229354935925 23.070782026770203 98-99 22.349370407198613 27.1853822578768 27.11937046845298 23.345876866471606 100-101 22.424615766591412 27.218506009769257 27.165010518397853 23.191867705241474 102-103 22.492959503542146 27.08234435617919 27.250197387078973 23.174498753199693 104-105 22.518522541697415 27.023452153384575 27.25042296693079 23.20760233798723 106-107 22.297080015215208 27.16564057928049 27.363752725496767 23.17352668000754 108-109 22.263391172680098 27.096043370526274 27.15417264906168 23.48639280773195 110-111 22.190184135511714 27.237428289485372 27.26094980789739 23.311437767105524 112-113 22.274958254705712 27.293357220067634 27.26650710415272 23.16517742107393 114-115 22.336213771792664 27.353105601602735 27.110764095479574 23.199916531125027 116-117 21.99813782922342 27.298259727902547 27.408744443605016 23.294857999269016 118-119 22.21455177825325 27.18528532520198 27.15126857368741 23.44889432285736 120-121 22.213249234545163 27.359515172800347 27.15054778898664 23.276687803667855 122-123 22.226541842349747 27.342977611254604 27.192596298149073 23.237884248246573 124-125 22.383309499489272 27.19197139938713 26.982982635342186 23.44173646578141 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 559.0 1 553.5 2 639.5 3 712.0 4 619.5 5 510.5 6 463.0 7 549.0 8 727.0 9 831.5 10 821.5 11 837.0 12 954.0 13 1069.0 14 1227.5 15 1388.5 16 1542.5 17 1772.5 18 2013.5 19 2341.5 20 2838.0 21 3569.0 22 4614.5 23 5956.0 24 7538.5 25 9800.0 26 13197.5 27 16933.0 28 20432.0 29 24655.5 30 29632.0 31 34678.5 32 40559.0 33 46781.5 34 52854.0 35 57862.5 36 63163.5 37 68467.0 38 71318.0 39 73571.0 40 75387.5 41 77330.0 42 79321.0 43 81356.0 44 84117.5 45 86269.5 46 87194.5 47 86902.0 48 87457.5 49 87441.5 50 85425.0 51 83874.0 52 82765.0 53 81956.5 54 79814.0 55 74857.0 56 69237.5 57 64057.0 58 59213.5 59 53177.0 60 45552.5 61 37474.0 62 29680.0 63 23065.5 64 17513.5 65 13304.5 66 10484.5 67 8360.0 68 6456.0 69 4895.5 70 3746.5 71 2946.5 72 2365.0 73 1936.0 74 1511.5 75 1124.0 76 829.5 77 605.5 78 449.5 79 314.0 80 229.0 81 168.5 82 116.0 83 79.5 84 52.5 85 39.0 86 28.5 87 22.5 88 21.0 89 16.5 90 15.5 91 11.0 92 5.5 93 4.5 94 5.0 95 5.5 96 4.0 97 3.0 98 4.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09641130262246093 2 0.032504615369537866 3 0.042958361016022406 4 0.050921175082680556 5 0.05353461149430169 6 0.0039201546174317025 7 0.002450096635894814 8 0.0028584460752106165 9 0.00706444530016338 10-11 0.06680596827206527 12-13 0.006737765748710739 14-15 0.011515454188705627 16-17 0.021315840732284885 18-19 0.019723277918953255 20-21 0.13353026665626738 22-23 0.017109841507332118 24-25 0.01576228835758997 26-27 0.007595299571273924 28-29 0.004859358327858047 30-31 0.0021234170844421723 32-33 0.0065335910290528374 34-35 0.018518647072971635 36-37 0.0030422033229027274 38-39 0.009269532272468713 40-41 0.031422489355350997 42-43 0.015068094310753108 44-45 0.11464410508791152 46-47 0.0044714263605080354 48-49 0.009800386543579256 50-51 0.011719628908363528 52-53 0.013005929642208305 54-55 0.010739590254005602 56-57 0.00692152299640285 58-59 0.01596646307724787 60-61 0.0349138770615011 62-63 0.007921979122726566 64-65 0.007615717043239714 66-67 0.009228697328537133 68-69 0.00884076536118712 70-71 0.0047981059119606775 72-73 8.371163505973948E-4 74-75 0.0011637959020500367 76-77 0.00865700811349501 78-79 0.025623927317066596 80-81 0.0034505527622185296 82-83 0.005043115575550159 84-85 0.0028584460752106165 86-87 0.003981407033329073 88-89 0.006186494005634405 90-91 0.005533134902729122 92-93 0.0032259605705948387 94-95 0.004614348664268566 96-97 0.0016742327011947896 98-99 0.0031851256266632586 100-101 0.0032055430986290484 102-103 5.921066870079135E-4 104-105 0.004491843832473826 106-107 7.146115188026541E-4 108-109 0.0012658832618789872 110-111 0.0025113490517921847 112-113 0.0042468341688843446 114-115 0.0026951062994842955 116-117 0.00522687282324227 118-119 0.003654727481876431 120-121 0.0071665326599923305 122-123 0.004410173944610665 124-125 0.056474727457375466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2448883.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.78614076881407 #Duplication Level Percentage of deduplicated Percentage of total 1 81.16511523305844 51.772094657728815 2 10.76405221904831 13.73194700174162 3 3.522032936994791 6.7397066613464816 4 1.5434343852543697 3.9379889186105297 5 0.8098351940095915 2.5828130842317822 6 0.4935997236234671 1.8890892872696519 7 0.33092855624389506 1.4776058831095447 8 0.22885901933813457 1.1678426898971994 9 0.16005938291939587 0.918861329723949 >10 0.8987837225945289 10.400008598748082 >50 0.05940599853422357 2.577343298252045 >100 0.02342740689645174 2.4478794420731065 >500 1.9980920759668099E-4 0.09346376044622445 >1k 2.664122767955603E-4 0.26335538682096343 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.166988786316047E-5 0.0 0.0 0.0 0.0 3 8.166988786316047E-5 0.0 0.0 0.0 0.0 4 8.166988786316047E-5 0.0 0.0 0.0 0.0 5 8.166988786316047E-5 0.0 0.0 0.0 0.0 6 8.166988786316047E-5 0.0 0.0 0.0 0.0 7 3.2667955145264187E-4 0.0 0.0 0.0 0.0 8 3.2667955145264187E-4 0.0 0.0 0.0 0.0 9 3.2667955145264187E-4 0.0 0.0 0.0 0.0 10-11 3.2667955145264187E-4 0.0 0.0 0.0 0.0 12-13 3.6751449538422213E-4 0.0 0.0 0.0 0.0 14-15 5.716892150421233E-4 0.0 0.0 0.0 0.0 16-17 5.921066870079135E-4 0.0 0.0 2.0417471965790117E-5 4.0834943931580234E-5 18-19 6.737765748710739E-4 0.0 0.0 4.0834943931580234E-5 4.0834943931580234E-5 20-21 7.350289907684443E-4 0.0 0.0 1.0208735982895059E-4 4.0834943931580234E-5 22-23 7.350289907684443E-4 0.0 0.0 2.2459219162369127E-4 4.0834943931580234E-5 24-25 9.392037104263454E-4 0.0 0.0 2.8584460752106167E-4 4.0834943931580234E-5 26-27 9.596211823921355E-4 0.0 0.0 3.2667955145264187E-4 4.0834943931580234E-5 28-29 0.001225048317947407 0.0 4.0834943931580234E-5 6.737765748710739E-4 4.0834943931580234E-5 30-31 0.0014292230376053083 0.0 4.0834943931580234E-5 0.002756358715381666 4.0834943931580234E-5 32-33 0.0014292230376053083 0.0 4.0834943931580234E-5 0.0077178044030686635 4.0834943931580234E-5 34-35 0.0015313103974342588 0.0 4.0834943931580234E-5 0.014557657511608353 4.0834943931580234E-5 36-37 0.0017150676451263699 0.0 4.0834943931580234E-5 0.025174742933819216 4.0834943931580234E-5 38-39 0.0020009122526474313 0.0 4.0834943931580234E-5 0.046327243890377776 4.0834943931580234E-5 40-41 0.0020825821405105922 0.0 8.166988786316047E-5 0.07444210278727077 4.0834943931580234E-5 42-43 0.002307174332134283 0.0 8.166988786316047E-5 0.10218944718877954 4.0834943931580234E-5 44-45 0.0025317665237579745 0.0 8.166988786316047E-5 0.13314233468891734 4.0834943931580234E-5 46-47 0.002817611131279036 0.0 8.166988786316047E-5 0.17387519126066864 4.0834943931580234E-5 48-49 0.002940115963073777 0.0 8.166988786316047E-5 0.21599643592609363 4.0834943931580234E-5 50-51 0.002940115963073777 0.0 8.166988786316047E-5 0.2651208734757847 4.0834943931580234E-5 52-53 0.002940115963073777 0.0 8.166988786316047E-5 0.3141636411376125 4.0834943931580234E-5 54-55 0.002940115963073777 0.0 8.166988786316047E-5 0.3685149515105458 4.0834943931580234E-5 56-57 0.0030626207948685176 0.0 8.166988786316047E-5 0.42795021240296083 4.0834943931580234E-5 58-59 0.0030626207948685176 0.0 8.166988786316047E-5 0.5011672668722842 4.0834943931580234E-5 60-61 0.0030626207948685176 0.0 8.166988786316047E-5 0.5808566599547631 4.0834943931580234E-5 62-63 0.0031034557388000977 0.0 8.166988786316047E-5 0.6661812752998 4.0834943931580234E-5 64-65 0.0032259605705948387 0.0 8.166988786316047E-5 0.7530984534581685 4.0834943931580234E-5 66-67 0.0033484654023895792 0.0 8.166988786316047E-5 0.8527152991792585 4.0834943931580234E-5 68-69 0.0035118051781159003 0.0 8.166988786316047E-5 0.9702382678143464 4.0834943931580234E-5 70-71 0.0036751449538422213 0.0 8.166988786316047E-5 1.1087912325741982 4.0834943931580234E-5 72-73 0.003858902201534332 0.0 8.166988786316047E-5 1.251141847119687 4.0834943931580234E-5 74-75 0.0039201546174317025 0.0 8.166988786316047E-5 1.3932066170576545 4.0834943931580234E-5 76-77 0.0039201546174317025 0.0 8.166988786316047E-5 1.5520545489515016 4.0834943931580234E-5 78-79 0.0039201546174317025 0.0 8.166988786316047E-5 1.7327083409048125 4.0834943931580234E-5 80-81 0.004573513720336986 0.0 8.166988786316047E-5 1.9433962341198008 4.0834943931580234E-5 82-83 0.004655183608200147 0.0 8.166988786316047E-5 2.1672533967527237 4.0834943931580234E-5 84-85 0.004818523383926468 0.0 8.166988786316047E-5 2.3869249776326598 4.0834943931580234E-5 86-87 0.005022698103584369 0.0 8.166988786316047E-5 2.612211363303188 4.0834943931580234E-5 88-89 0.005104367991447529 0.0 8.166988786316047E-5 2.871697014516414 4.0834943931580234E-5 90-91 0.005512717430763332 0.0 8.166988786316047E-5 3.171445920446179 4.0834943931580234E-5 92-93 0.005512717430763332 0.0 8.166988786316047E-5 3.490305580135923 4.0834943931580234E-5 94-95 0.005512717430763332 0.0 8.166988786316047E-5 3.808450628306865 4.0834943931580234E-5 96-97 0.005512717430763332 0.0 8.166988786316047E-5 4.124043492482082 4.0834943931580234E-5 98-99 0.005798562038284393 0.0 8.166988786316047E-5 4.467975807745818 4.0834943931580234E-5 100-101 0.006104824117771246 0.0 8.166988786316047E-5 4.8697304036166695 4.0834943931580234E-5 102-103 0.006472338613155467 0.0 8.166988786316047E-5 5.302703314123214 4.0834943931580234E-5 104-105 0.007595299571273924 0.0 8.166988786316047E-5 5.737330039858989 4.0834943931580234E-5 106-107 0.008064901426487097 0.0 8.166988786316047E-5 6.155296108470678 4.0834943931580234E-5 108-109 0.008166988786316047 0.0 8.166988786316047E-5 6.595598891412942 4.0834943931580234E-5 110-111 0.008248658674179208 0.0 8.166988786316047E-5 7.0936627025464265 4.0834943931580234E-5 112-113 0.008371163505973948 0.0 8.166988786316047E-5 7.644648601015239 4.0834943931580234E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6325 0.0 52.992165 1 GGTATCA 3500 0.0 51.879475 1 ATCAACG 7420 0.0 42.499832 3 TCAACGC 7485 0.0 42.03361 4 CAACGCA 7500 0.0 41.550594 5 AACGCAG 7700 0.0 39.986603 6 TATCAAC 8275 0.0 39.83347 2 GTACATG 11090 0.0 36.128284 1 TACATGG 11160 0.0 34.121033 2 ACGCAGA 9160 0.0 32.899364 7 CGCAGAG 9115 0.0 32.08366 8 ACATGGG 11125 0.0 31.28752 3 GCAGAGT 10015 0.0 27.773146 9 ACGCATA 270 5.820766E-11 26.47138 8 CATGGGA 6130 0.0 26.148478 4 CATGGGG 5595 0.0 23.96277 4 AGAGTAC 9890 0.0 22.990166 10-11 CAGAGTA 10470 0.0 21.830292 10-11 CGCATAG 275 5.4624252E-8 21.658403 9 GAGTACT 4830 0.0 21.182264 12-13 >>END_MODULE