FastQCFastQC Report
Thu 26 May 2016
SRR1364516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448883
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51920.21201502889276455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47040.19208757625415343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30050.1227090065143986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48650.060.4663541
GTATCAA83100.049.544761
ATCAACG105850.037.048363
TATCAAC111400.036.486582
TCAACGC106350.036.425864
CAACGCA108850.035.5252845
AACGCAG110900.034.3321536
GTACATG106400.032.6385651
TACATGG109150.031.2324642
CGCAGAG131650.029.000028
ACGCAGA131300.028.8167517
ACATGGG109400.028.4372183
GCAGAGT140300.025.9404729
CATGGGA61750.024.2253884
GAGTACT88000.023.88909712-13
AGAGTAC137950.023.68857810-11
AGTACTT92550.023.26082612-13
GTACTTT92900.023.0133914-15
CAGAGTA144400.022.6716510-11
CATGGGG50700.021.6293684
Produced by FastQC (version 0.11.5)