##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364516_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2448883 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.943160616493316 31.0 28.0 31.0 25.0 34.0 2 29.16843189323459 31.0 28.0 34.0 25.0 34.0 3 29.245161977930348 31.0 28.0 34.0 25.0 34.0 4 32.63924082939038 35.0 32.0 37.0 26.0 37.0 5 31.762552559677207 35.0 32.0 37.0 20.0 37.0 6 31.799407729973215 35.0 32.0 37.0 21.0 37.0 7 31.21081325649286 35.0 31.0 37.0 17.0 37.0 8 31.360422282322187 35.0 32.0 37.0 17.0 37.0 9 31.924598684379777 37.0 30.0 39.0 11.0 39.0 10-11 32.13579701439391 37.0 31.0 39.0 15.5 39.0 12-13 31.87293553836586 37.0 30.5 39.0 12.5 39.0 14-15 32.833677231619475 38.0 31.0 40.0 12.0 41.0 16-17 32.901237625480675 38.0 31.0 40.0 12.0 41.0 18-19 32.75192628639261 38.0 31.0 40.0 10.5 41.0 20-21 32.61935992858785 38.0 30.5 40.0 10.5 41.0 22-23 32.59151110935067 38.0 31.0 40.0 10.0 41.0 24-25 32.390435966111895 38.0 30.0 40.0 10.0 41.0 26-27 32.48982821964137 37.0 31.0 40.0 10.0 41.0 28-29 32.38213381366117 37.0 30.5 40.0 9.0 41.0 30-31 31.84160696938155 37.0 30.0 40.0 8.0 41.0 32-33 31.78273420984179 37.0 30.0 40.0 8.0 41.0 34-35 31.57384346250923 37.0 30.0 40.0 2.0 41.0 36-37 31.57058565068237 37.0 30.0 40.0 2.0 41.0 38-39 31.32443730468136 37.0 30.0 40.0 2.0 41.0 40-41 31.0266058035439 36.0 29.5 40.0 2.0 41.0 42-43 30.90539053927852 36.0 29.0 40.0 2.0 41.0 44-45 30.831883556707282 36.0 29.0 40.0 2.0 41.0 46-47 30.668385953922666 36.0 28.5 40.0 2.0 41.0 48-49 30.371284785757425 36.0 28.0 40.0 2.0 41.0 50-51 30.01481348843534 36.0 27.0 40.0 2.0 41.0 52-53 29.934586707490723 35.5 27.0 40.0 2.0 41.0 54-55 29.46185117868024 35.0 26.0 40.0 2.0 41.0 56-57 29.491648845616552 35.0 26.0 39.0 2.0 41.0 58-59 29.25052381024328 35.0 26.0 39.0 2.0 41.0 60-61 28.96278915734235 35.0 26.0 39.0 2.0 41.0 62-63 28.69259229616115 34.0 25.5 39.0 2.0 41.0 64-65 28.200668631371933 34.0 23.5 38.5 2.0 40.0 66-67 27.80562505436152 34.0 22.5 38.0 2.0 40.0 68-69 27.613389859785052 34.0 22.5 37.5 2.0 40.0 70-71 27.714330778563124 34.0 24.5 37.0 2.0 40.0 72-73 27.483108625442703 34.0 23.5 37.0 2.0 39.0 74-75 27.10625007401334 34.0 22.5 36.0 2.0 39.0 76-77 27.037751497315305 33.5 23.5 36.0 2.0 39.0 78-79 26.626539120080462 33.0 22.0 35.5 2.0 37.5 80-81 26.404075449909204 33.0 22.0 35.0 2.0 37.0 82-83 26.268745178924434 33.0 22.0 35.0 2.0 37.0 84-85 25.815430545273088 33.0 20.0 35.0 2.0 36.0 86-87 25.701176413899724 33.0 20.0 35.0 2.0 36.0 88-89 25.668983573327104 33.0 20.0 35.0 2.0 36.0 90-91 25.504181702433314 33.0 20.0 35.0 2.0 35.0 92-93 25.534983092291462 33.0 20.0 35.0 2.0 35.0 94-95 25.553039487799133 33.0 20.0 35.0 2.0 35.0 96-97 25.43448584517921 33.0 20.0 35.0 2.0 35.0 98-99 25.295064116987213 33.0 19.0 35.0 2.0 35.0 100-101 25.20027314493996 33.0 18.5 35.0 2.0 35.0 102-103 24.089146153572877 30.5 17.5 34.0 2.0 35.0 104-105 25.040905180035143 32.0 18.5 35.0 2.0 35.0 106-107 25.1280185700991 33.0 18.0 35.0 2.0 35.0 108-109 25.205693983746876 33.0 18.0 35.0 2.0 35.0 110-111 25.136969998158342 33.0 18.0 35.0 2.0 35.0 112-113 25.09047431012425 33.0 17.5 35.0 2.0 35.0 114-115 25.040382696927537 33.0 17.0 35.0 2.0 35.0 116-117 24.9665900739235 33.0 12.5 35.0 2.0 35.0 118-119 24.84351906563115 33.0 4.5 35.0 2.0 35.0 120-121 24.682573646842254 33.0 2.0 35.0 2.0 35.0 122-123 24.407022303638026 33.0 2.0 35.0 2.0 35.0 124-125 23.630920709564318 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9512170322892288 1101 2 0.985407608731844 1101 3 0.9253535773812764 1101 4 0.9239930696988381 1101 5 1.1090155931236794 1101 6 1.2111274776708605 1101 7 1.6699787876792875 1101 8 1.3911221541803442 1101 9 2.0419893632386668 1101 10-11 1.8953607515354634 1101 12-13 2.0344721708779296 1101 14-15 1.893478097534846 1101 16-17 1.895369749859242 1101 18-19 1.8474733303376922 1101 20-21 2.0716247293881125 1101 22-23 2.0688188197070687 1101 24-25 2.150811840723236 1101 26-27 2.007428549910628 1101 28-29 1.9241809883896508 1101 30-31 2.51767971029377 1101 32-33 2.362979199135463 1101 34-35 2.373699134095741 1101 36-37 2.1497495465894616 1101 38-39 2.3614210297591463 1101 40-41 2.490514561421559 1101 42-43 2.467515617796863 1101 44-45 2.2498475327137477 1101 46-47 2.003202155111463 1101 48-49 2.152210191948644 1101 50-51 2.399552245158258 1101 52-53 2.30009957870168 1101 54-55 2.67594413405088 1101 56-57 2.257512968312465 1101 58-59 2.375785506208537 1101 60-61 2.3409291218065675 1101 62-63 2.394631366248511 1101 64-65 2.536163307228719 1101 66-67 2.7127726560307295 1101 68-69 2.75256817240966 1101 70-71 2.290758937399918 1101 72-73 2.5269973139963717 1101 74-75 2.5076711653353243 1101 76-77 2.343398741506217 1101 78-79 2.3841175050134638 1101 80-81 2.4418811937229314 1101 82-83 2.376122735173979 1101 84-85 2.6475706104420063 1101 86-87 2.440248173971206 1101 88-89 2.288254652687854 1101 90-91 2.3324046365148092 1101 92-93 2.2055102581567283 1101 94-95 2.1409856419695004 1101 96-97 2.0956930058713183 1101 98-99 2.2426923304256157 1101 100-101 2.3683941701744047 1101 102-103 2.0932258312699794 1101 104-105 2.126896763012361 1101 106-107 2.3856208261383074 1101 108-109 2.298239346792041 1101 110-111 2.2300085103580187 1101 112-113 2.093764875678236 1101 114-115 2.0947309646165984 1101 116-117 2.202806154747279 1101 118-119 2.1025822545521358 1101 120-121 2.0002340852400984 1101 122-123 2.0653102565562875 1101 124-125 1.8883343175918483 1102 1 0.5420294245328847 1102 2 -1.5558188951468495 1102 3 0.16448412187254746 1102 4 0.5037225964900074 1102 5 1.07986460532085 1102 6 0.6413850927585365 1102 7 1.392991490732232 1102 8 1.087291694196658 1102 9 1.7385607960046734 1102 10-11 1.4225824094562185 1102 12-13 1.5996785048789413 1102 14-15 1.5884095387301898 1102 16-17 1.5478473697243658 1102 18-19 1.5920417375914582 1102 20-21 1.7681213425088416 1102 22-23 1.705704973645446 1102 24-25 1.9118300179591614 1102 26-27 1.5885820585658479 1102 28-29 1.6330363770404333 1102 30-31 1.8890638607325698 1102 32-33 1.5088686376447988 1102 34-35 1.6972908884432307 1102 36-37 1.3058490184094111 1102 38-39 1.257752619110395 1102 40-41 1.506212808783797 1102 42-43 1.2443857724888687 1102 44-45 1.1629664406665547 1102 46-47 0.9531338195805645 1102 48-49 1.0753712573498113 1102 50-51 1.245455470057017 1102 52-53 1.0646726639992927 1102 54-55 1.3451766901774675 1102 56-57 1.0230411988312937 1102 58-59 0.9411072238014988 1102 60-61 0.9491660421703259 1102 62-63 1.3705958323841543 1102 64-65 1.4370814899394375 1102 66-67 1.5939952648529783 1102 68-69 1.5156619926510473 1102 70-71 0.9572058476429532 1102 72-73 1.2570444400503114 1102 74-75 1.4763718558629222 1102 76-77 1.2612433683622584 1102 78-79 1.1536330453252326 1102 80-81 1.160429071720035 1102 82-83 0.8835222719268252 1102 84-85 0.9771889973717443 1102 86-87 1.1168232249953718 1102 88-89 1.183568090800808 1102 90-91 1.0097645865259608 1102 92-93 1.0665434910780434 1102 94-95 1.0488167087684168 1102 96-97 1.0849700928768158 1102 98-99 0.8367947607756712 1102 100-101 0.6840290827260276 1102 102-103 0.5374476519053033 1102 104-105 0.7996414075773721 1102 106-107 1.0619107443497668 1102 108-109 0.8510010366256537 1102 110-111 0.731561104019061 1102 112-113 0.78090020159215 1102 114-115 1.0932984952915028 1102 116-117 0.6781809717316243 1102 118-119 0.22397634526061339 1102 120-121 0.5362702390714311 1102 122-123 0.9444379535426108 1102 124-125 0.9526594849171737 1103 1 0.7766742400426594 1103 2 0.7064452501926475 1103 3 0.5566047345804215 1103 4 0.6565090401336491 1103 5 0.9186612228877564 1103 6 0.9995269310817001 1103 7 1.156926450549907 1103 8 1.165238184420076 1103 9 1.8095650994781032 1103 10-11 1.5922914899610774 1103 12-13 1.5891654229182954 1103 14-15 1.3117287688190018 1103 16-17 1.3463518805312589 1103 18-19 1.5882976646819742 1103 20-21 1.7042174769983092 1103 22-23 1.6747580655565812 1103 24-25 2.059442873044837 1103 26-27 1.4946441614289867 1103 28-29 1.585246376552277 1103 30-31 2.247133503603443 1103 32-33 2.0338354700030727 1103 34-35 2.0816232644252715 1103 36-37 2.0640044581120804 1103 38-39 2.0117120789269656 1103 40-41 2.209362434391739 1103 42-43 2.1353675171681346 1103 44-45 2.029552671851466 1103 46-47 2.0290402932660463 1103 48-49 2.0315664762897505 1103 50-51 2.202645737287572 1103 52-53 1.9557911078735337 1103 54-55 2.305238592446912 1103 56-57 2.1787532337141897 1103 58-59 2.1386292215197287 1103 60-61 2.2046280127208284 1103 62-63 2.302802018026533 1103 64-65 2.419529694081369 1103 66-67 2.5595700286127823 1103 68-69 2.629247143417313 1103 70-71 2.4142708770873504 1103 72-73 2.3790590191146492 1103 74-75 2.3614981152756513 1103 76-77 2.2094277860095985 1103 78-79 2.4430750087490978 1103 80-81 2.382746361441832 1103 82-83 2.3021032794509146 1103 84-85 2.4903376702738704 1103 86-87 2.304479919779425 1103 88-89 2.1650284814284646 1103 90-91 2.2084532871228113 1103 92-93 2.063743366091572 1103 94-95 1.946201535127308 1103 96-97 1.8590088367033601 1103 98-99 2.0061598467046338 1103 100-101 2.0096637382490066 1103 102-103 1.8519541552457532 1103 104-105 1.81350582832577 1103 106-107 1.925301565215868 1103 108-109 1.7820886496909267 1103 110-111 1.8351528201544625 1103 112-113 1.8750610821512446 1103 114-115 1.6903353898330629 1103 116-117 1.820566434370054 1103 118-119 1.8514928574952698 1103 120-121 1.798987700265041 1103 122-123 1.924011133724104 1103 124-125 1.7412463739336488 1104 1 0.7072186875607862 1104 2 0.5469324225425929 1104 3 0.5304723923588917 1104 4 0.5357382984102514 1104 5 0.8458870262121749 1104 6 0.8419341029039025 1104 7 1.225316635284873 1104 8 0.9353522887618695 1104 9 1.4790423924028921 1104 10-11 1.0406193258018064 1104 12-13 1.2229607478339481 1104 14-15 1.3187245020419667 1104 16-17 1.132311304489825 1104 18-19 1.1492331074692572 1104 20-21 1.4232607211198243 1104 22-23 1.4406580947971932 1104 24-25 1.6426105000339106 1104 26-27 1.4091164200645423 1104 28-29 1.3438742563184505 1104 30-31 1.895126692936465 1104 32-33 1.7861989902855733 1104 34-35 1.717663512352356 1104 36-37 1.671511414408485 1104 38-39 1.800810171776721 1104 40-41 1.9080583233112698 1104 42-43 1.8073568637774713 1104 44-45 1.5671905626900617 1104 46-47 1.6645373527851 1104 48-49 1.3440957760538907 1104 50-51 1.5961058793547664 1104 52-53 1.497426565675493 1104 54-55 1.7336822406022208 1104 56-57 1.6438636589592583 1104 58-59 1.792915810176062 1104 60-61 1.7408746084270348 1104 62-63 1.872332643871644 1104 64-65 2.012544802398537 1104 66-67 1.999617494768458 1104 68-69 2.067629228546636 1104 70-71 1.8372214423890583 1104 72-73 2.065510887050351 1104 74-75 2.2115297507750427 1104 76-77 1.9765646614576546 1104 78-79 2.0086406209325816 1104 80-81 1.9813625736457645 1104 82-83 1.8928636414034763 1104 84-85 2.198969916771489 1104 86-87 2.1372551504166992 1104 88-89 1.9047291258547752 1104 90-91 1.9367489468212078 1104 92-93 1.652817086987021 1104 94-95 1.6418330039308522 1104 96-97 1.6956724301662582 1104 98-99 1.7355345336300694 1104 100-101 1.7502854475153242 1104 102-103 1.6452160591779474 1104 104-105 1.6157529813100808 1104 106-107 1.7518922780026749 1104 108-109 1.6192234414408588 1104 110-111 1.637290697297729 1104 112-113 1.6493726264268211 1104 114-115 1.5697717272625198 1104 116-117 1.6187701661256888 1104 118-119 1.6274065763313992 1104 120-121 1.622575586077236 1104 122-123 1.8164732374503991 1104 124-125 1.5844613910728889 1105 1 0.6002071018550943 1105 2 0.6032877449480374 1105 3 0.46415107429181646 1105 4 0.6467395112283185 1105 5 0.8354094867770172 1105 6 0.9755039612389247 1105 7 1.247380951931401 1105 8 1.0956620781281288 1105 9 1.8331025469789743 1105 10-11 1.4903392732658816 1105 12-13 1.549110764099627 1105 14-15 1.4737790559959407 1105 16-17 1.4254417809406021 1105 18-19 1.6272000434970693 1105 20-21 1.7117217980034027 1105 22-23 1.7356806974456163 1105 24-25 1.705876806679285 1105 26-27 1.4873270055132082 1105 28-29 1.4778826521797512 1105 30-31 1.8971184348012287 1105 32-33 1.7476253323396982 1105 34-35 1.8567683443229832 1105 36-37 1.5564547474165522 1105 38-39 1.7806962720301591 1105 40-41 1.8803075914986458 1105 42-43 1.854670483900108 1105 44-45 1.9339154081447028 1105 46-47 1.745146072016933 1105 48-49 1.8434859024375676 1105 50-51 1.9710908244950751 1105 52-53 1.8927148196275105 1105 54-55 2.0480257465679443 1105 56-57 1.8539548153310683 1105 58-59 1.878549709419726 1105 60-61 1.6823701735475325 1105 62-63 1.600250147097995 1105 64-65 2.0076273816078185 1105 66-67 2.2675879610983642 1105 68-69 2.1799680201089444 1105 70-71 2.0003515445220827 1105 72-73 2.2477723762835 1105 74-75 2.290190731907572 1105 76-77 2.114981255963386 1105 78-79 2.1881218722568008 1105 80-81 2.1088848430513885 1105 82-83 2.0007029050776097 1105 84-85 2.2316671432913004 1105 86-87 2.1961398678375232 1105 88-89 1.9934930786558809 1105 90-91 2.0796501036487705 1105 92-93 1.887792908489459 1105 94-95 1.7160145303214662 1105 96-97 1.8359199313452876 1105 98-99 1.8963859764892455 1105 100-101 1.9335525227979105 1105 102-103 1.8266237610655836 1105 104-105 1.4895427527490916 1105 106-107 1.632614142630807 1105 108-109 1.6883340339044146 1105 110-111 1.8360582061174107 1105 112-113 1.8891213563538507 1105 114-115 1.862871593951109 1105 116-117 1.7608870415554563 1105 118-119 1.8059206020315948 1105 120-121 1.8936607475074787 1105 122-123 1.9419199721066924 1105 124-125 1.7195778460381064 1106 1 0.2602397067118467 1106 2 0.25045057466960685 1106 3 0.32371026826803373 1106 4 0.18147185699214674 1106 5 0.39575457505176104 1106 6 0.5711184382631878 1106 7 0.7703673902052621 1106 8 0.480114605239887 1106 9 1.1672757261536368 1106 10-11 0.7424044282052833 1106 12-13 0.6875445056902301 1106 14-15 0.8025217891553282 1106 16-17 0.7392891668749471 1106 18-19 0.8441225104657164 1106 20-21 0.6548298319382369 1106 22-23 0.7916265620862433 1106 24-25 0.6201911347293958 1106 26-27 0.7758945014750438 1106 28-29 0.8180588022790616 1106 30-31 1.1426048131659101 1106 32-33 0.9092266661462212 1106 34-35 0.8737874005765569 1106 36-37 0.9289681956005325 1106 38-39 1.152829653538081 1106 40-41 1.374023938944493 1106 42-43 1.2500876742966085 1106 44-45 1.1304988875898552 1106 46-47 1.0663257707365297 1106 48-49 1.0268479256104328 1106 50-51 1.3503136369970328 1106 52-53 1.0031226912940632 1106 54-55 1.5065230931619382 1106 56-57 1.2353063292930457 1106 58-59 1.0724531446690335 1106 60-61 1.4809920698955281 1106 62-63 1.5502861425289218 1106 64-65 1.5632513413990594 1106 66-67 1.542056081713227 1106 68-69 1.4953904111926306 1106 70-71 1.337022936406271 1106 72-73 1.2593432990503004 1106 74-75 1.5328772833812536 1106 76-77 1.1824525765841791 1106 78-79 1.1254722776137065 1106 80-81 1.2594274775603296 1106 82-83 1.1079941800761404 1106 84-85 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-4.467272501872941 2216 100-101 -4.8249475603068745 2216 102-103 -4.860591681861401 2216 104-105 -4.630594688121317 2216 106-107 -4.764586439747848 2216 108-109 -4.7697590047413065 2216 110-111 -4.389706519174428 2216 112-113 -4.458244959388406 2216 114-115 -4.329637608252195 2216 116-117 -4.264308669438982 2216 118-119 -4.446814897105558 2216 120-121 -4.3948898098475695 2216 122-123 -4.499659393574934 2216 124-125 -4.362368231400801 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 124741.0 3 41607.0 4 20205.0 5 16169.0 6 16327.0 7 19016.0 8 21155.0 9 22725.0 10 24067.0 11 26345.0 12 28747.0 13 31171.0 14 31835.0 15 25844.0 16 18442.0 17 15663.0 18 13592.0 19 12695.0 20 12569.0 21 13477.0 22 16436.0 23 22102.0 24 31310.0 25 41563.0 26 50830.0 27 57725.0 28 65313.0 29 73627.0 30 82467.0 31 93157.0 32 108777.0 33 129498.0 34 156937.0 35 199702.0 36 281707.0 37 366628.0 38 133910.0 39 802.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.8795557779282 19.892746073324112 13.966433175383347 24.261264973364334 2 15.710371970195341 20.606489744899605 39.12990392747049 24.553234357434565 3 18.97946941523952 25.573496161311095 29.038994513008582 26.4080399104408 4 12.730171405848695 17.278166107150543 36.485783272117935 33.505879214882825 5 13.031328977333748 38.943428493725506 34.007463811051814 14.017778717888932 6 31.629915867696308 37.482343976697926 17.03627085187071 13.851469303735053 7 27.74953060580856 32.39992385110767 21.110809711108097 18.739735831975672 8 25.977394591738356 36.25812258078479 19.21288195475243 18.55160087272442 9 25.784653656381295 15.88111804443087 20.09140493849645 38.24282336069139 10-11 24.912541187414426 27.171422539484585 27.01755546544586 20.898480807655133 12-13 25.67418381182983 24.28427659549706 27.812559147214316 22.22898044545879 14-15 22.63007868720046 25.565326261238074 25.814685813701686 25.989909237859777 16-17 22.072447051727565 28.74876749926443 26.475984076161964 22.702801372846046 18-19 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28.54580149710416 22.693917702964768 44-45 22.310333569701964 26.672427173721964 28.437351525210623 22.57988773136545 46-47 22.500674289076763 26.995635343650587 27.9338618888547 22.569828478417953 48-49 22.55429212187439 27.03409147521195 27.685797029011606 22.725819373902052 50-51 22.543171954728557 27.10187464011881 27.45310029407361 22.90185311107902 52-53 22.66438126817157 27.208050210708567 27.39295106334324 22.734617457776615 54-55 22.506877674012053 27.03337371506852 27.45882340913854 23.000925201780888 56-57 22.339142051467277 27.252803479473137 27.461205168982595 22.946849300076995 58-59 22.525678033617776 26.993551754003764 27.483754838430418 22.99701537394804 60-61 22.655022718521057 27.121181371261915 27.544823497080095 22.67897241313693 62-63 22.41839903171588 27.14139757634956 27.657452341245016 22.78275105068954 64-65 22.300200857982926 26.903017528030936 27.555954097893085 23.240827516093056 66-67 22.177583352313988 27.14318836198136 27.340539513639005 23.338688772065648 68-69 22.225405129930696 26.94281465050448 27.62417695859761 23.207603260967215 70-71 22.21184534017309 26.950189673024084 27.664778886316448 23.173186100486376 72-73 22.376246241914266 27.0344744728055 27.260582814376615 23.32869647090362 74-75 22.23523110839862 27.191181145836037 27.255662932181618 23.317924813583726 76-77 22.37829416845153 27.026586111354987 27.395373718523295 23.19974600167018 78-79 22.3912470006354 27.052390262866027 27.322455583269225 23.23390715322935 80-81 22.169599440723008 27.16905633441628 27.376763870445053 23.284580354415656 82-83 22.412447659703748 27.14739158096075 27.251582153132986 23.188578606202515 84-85 22.1878550726709 27.045172645073823 27.174415269005355 23.59255701324992 86-87 22.191024254990296 27.03991930996957 27.384526175768286 23.38453025927185 88-89 22.334934900108298 27.002999772954837 27.18624890356788 23.475816423368993 90-91 22.222108791521336 27.145243951665183 27.37618659005466 23.25646066675882 92-93 22.24964130705321 27.161065026585234 27.279999787652958 23.309293878708594 94-95 22.089612457422835 26.858724863595846 27.406444573215577 23.645218105765746 96-97 22.246351806894353 26.83644946637455 27.315701676116817 23.601497050614277 98-99 21.926568153725594 26.770878804744857 27.242726581874265 24.05982645965528 100-101 21.991270608878807 26.819530037219458 27.387591785298714 23.80160756860302 102-103 22.063517212991577 26.72215670388689 27.42258809040006 23.791737992721476 104-105 22.020694762361824 26.609485436304464 27.51034605404388 23.859473747289833 106-107 21.70626537309599 26.671085012110634 27.49834056827191 24.12430904652146 108-109 21.895594848753493 26.623852589119203 27.24403330008008 24.236519262047228 110-111 21.663567972473956 26.72894272935271 27.362252392010884 24.245236906162447 112-113 21.82560561856734 26.734481327284037 27.411892903716616 24.028020150432003 114-115 21.79931940357261 26.797723777161124 27.262895325886532 24.140061493379733 116-117 21.55282632939181 26.731697676042504 27.411068638232205 24.304407356333478 118-119 21.52374130920014 26.57347069282203 27.36496336653798 24.53782463143985 120-121 21.48728944113887 26.75358097329295 27.378925400720068 24.380204184848115 122-123 21.58122716409591 26.761622511722305 27.26110032943934 24.396049994742448 124-125 21.44614084058391 26.594599892594097 27.2847338641009 24.674525402721088 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 184.0 1 158.5 2 281.5 3 453.5 4 334.0 5 224.0 6 433.0 7 933.0 8 1500.5 9 1783.0 10 1522.0 11 1171.5 12 1156.5 13 1276.5 14 1484.5 15 1737.5 16 2011.0 17 2355.5 18 2765.0 19 3207.5 20 3679.0 21 4423.0 22 5478.5 23 6696.0 24 8233.5 25 10320.0 26 13427.0 27 16887.5 28 19951.5 29 23885.5 30 28821.0 31 33679.5 32 39095.0 33 44958.5 34 51187.0 35 56458.0 36 61483.0 37 66582.5 38 69747.5 39 72720.5 40 74542.5 41 76022.0 42 77991.0 43 80206.0 44 82883.0 45 85369.0 46 86796.0 47 86923.5 48 88338.0 49 88265.5 50 86172.0 51 84442.5 52 83062.5 53 82626.5 54 81036.0 55 76257.5 56 70351.0 57 65250.5 58 60296.5 59 54140.5 60 46646.5 61 38248.0 62 30260.0 63 23621.0 64 17746.5 65 13271.5 66 10412.5 67 8283.0 68 6408.5 69 4789.0 70 3619.5 71 2844.5 72 2229.5 73 1916.0 74 1530.5 75 1092.5 76 785.0 77 524.0 78 364.0 79 246.5 80 160.0 81 109.5 82 75.5 83 48.5 84 29.5 85 18.5 86 14.0 87 10.0 88 6.0 89 3.5 90 2.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10367992264228222 2 1.6333977572632094E-4 3 0.0 4 0.1841247621874953 5 0.0 6 8.166988786316047E-5 7 0.04275418629636451 8 0.0 9 0.0 10-11 6.533591029052837E-4 12-13 5.921066870079135E-4 14-15 3.879319673500122E-4 16-17 0.004144746809055394 18-19 0.0014700579815368883 20-21 0.0032055430986290484 22-23 0.0016946501731605796 24-25 0.005043115575550159 26-27 0.004267251640850134 28-29 0.003634310009910641 30-31 8.371163505973948E-4 32-33 4.0834943931580234E-5 34-35 0.001225048317947407 36-37 0.0013067182058105675 38-39 5.921066870079135E-4 40-41 0.0014496405095710983 42-43 8.983687664947651E-4 44-45 0.0010004561263237159 46-47 3.0626207948685174E-4 48-49 4.0834943931580234E-5 50-51 0.04191706994576711 52-53 1.225048317947407E-4 54-55 0.1417176729145492 56-57 2.6542713555527153E-4 58-59 0.0 60-61 0.0 62-63 6.125241589737035E-4 64-65 0.06662221102437316 66-67 0.049634874348835775 68-69 0.0015925628133316293 70-71 0.0025930189396553447 72-73 0.07456460761906551 74-75 0.00528812523913964 76-77 0.0019600773087158512 78-79 0.0012658832618789872 80-81 2.8584460752106167E-4 82-83 2.0417471965790117E-4 84-85 2.0417471965790117E-5 86-87 2.2459219162369127E-4 88-89 0.0012658832618789872 90-91 2.6542713555527153E-4 92-93 0.0025113490517921847 94-95 0.003838484729568542 96-97 0.002776776187347456 98-99 0.0 100-101 0.001204630845981617 102-103 0.0014292230376053083 104-105 1.0208735982895059E-4 106-107 1.0208735982895059E-4 108-109 0.0 110-111 8.166988786316047E-5 112-113 1.0208735982895059E-4 114-115 6.125241589737035E-5 116-117 0.0 118-119 0.001184213374015827 120-121 0.002960533435039567 122-123 0.001041291070255296 124-125 0.009310367216400293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2448883.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.6940887104218 #Duplication Level Percentage of deduplicated Percentage of total 1 80.19764713679885 48.67523109687974 2 11.038634435442962 13.399597153333842 3 3.7963479730870087 6.912476419625187 4 1.6521553345734858 4.011042497599991 5 0.8955964238383104 2.7178704398589466 6 0.50178141295236 1.8273099354582798 7 0.35188103940440063 1.4949969314788287 8 0.2445631779736882 1.1874831375390165 9 0.18587626672284846 1.015343155947473 >10 1.0263312519557086 11.4732017085161 >50 0.07715863582218374 3.1883349849414357 >100 0.03099799076178197 3.1865169887858307 >500 6.859471109752133E-4 0.2659095545942168 >1k 2.743788443893427E-4 0.4285277979383385 >5k 6.859471109733568E-5 0.2161581975027546 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5192 0.21201502889276455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4704 0.19208757625415343 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3005 0.1227090065143986 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.166988786316047E-5 0.0 0.0 0.0 0.0 3 8.166988786316047E-5 0.0 0.0 0.0 0.0 4 8.166988786316047E-5 0.0 0.0 0.0 0.0 5 8.166988786316047E-5 0.0 0.0 0.0 0.0 6 1.225048317947407E-4 0.0 0.0 0.0 0.0 7 2.450096635894814E-4 0.0 0.0 0.0 0.0 8 3.2667955145264187E-4 0.0 0.0 0.0 0.0 9 3.2667955145264187E-4 0.0 0.0 0.0 0.0 10-11 3.6751449538422213E-4 0.0 0.0 2.0417471965790117E-5 0.0 12-13 4.0834943931580234E-4 0.0 0.0 4.0834943931580234E-5 0.0 14-15 4.0834943931580234E-4 0.0 0.0 4.0834943931580234E-5 0.0 16-17 4.696018552131727E-4 0.0 0.0 4.0834943931580234E-5 0.0 18-19 6.329416309394937E-4 0.0 0.0 1.0208735982895059E-4 0.0 20-21 7.350289907684443E-4 0.0 0.0 1.8375724769211107E-4 0.0 22-23 8.779512945289751E-4 0.0 0.0 3.47097023418432E-4 0.0 24-25 0.0011842133740158267 0.0 0.0 4.491843832473826E-4 0.0 26-27 0.0011842133740158267 0.0 0.0 5.716892150421232E-4 4.0834943931580234E-5 28-29 0.0016538152292289994 0.0 0.0 9.187862384605553E-4 4.0834943931580234E-5 30-31 0.0019600773087158512 0.0 0.0 0.0028584460752106165 4.0834943931580234E-5 32-33 0.0019804947806816415 0.0 0.0 0.007452377267513393 4.0834943931580234E-5 34-35 0.002102999612476382 0.0 0.0 0.01423097796015571 4.0834943931580234E-5 36-37 0.0023275918041000734 0.0 0.0 0.02446013141501656 4.0834943931580234E-5 38-39 0.0027155237714500853 0.0 0.0 0.04555137995567775 4.0834943931580234E-5 40-41 0.002756358715381666 0.0 0.0 0.07235952064676018 4.0834943931580234E-5 42-43 0.002940115963073777 0.0 0.0 0.0989430691462189 4.0834943931580234E-5 44-45 0.003185125626663258 0.0 0.0 0.12920176259951988 4.0834943931580234E-5 46-47 0.0035730575940132706 0.0 0.0 0.16946501731605795 4.0834943931580234E-5 48-49 0.0037159798977738014 0.0 0.0 0.21060622332712506 4.0834943931580234E-5 50-51 0.0037568148417053814 0.0 0.0 0.25913855418980813 4.0834943931580234E-5 52-53 0.0037568148417053814 0.0 0.0 0.3072421181412097 4.0834943931580234E-5 54-55 0.0038793196735001224 0.0 0.0 0.3598579433970508 4.0834943931580234E-5 56-57 0.004042659449226443 0.0 0.0 0.4177414764200658 4.0834943931580234E-5 58-59 0.004042659449226443 0.0 0.0 0.48889636622084437 4.0834943931580234E-5 60-61 0.004042659449226443 0.0 0.0 0.5653802162046941 4.0834943931580234E-5 62-63 0.004042659449226443 0.0 0.0 0.6454983761984545 4.0834943931580234E-5 64-65 0.004124329337089604 0.0 0.0 0.727556196028965 6.125241589737035E-5 66-67 0.004287669112815925 0.0 0.0 0.8217011592632233 8.166988786316047E-5 68-69 0.004410173944610666 0.0 0.0 0.9345076918742137 8.166988786316047E-5 70-71 0.0044918438324738266 0.0 0.0 1.0671600072359522 8.166988786316047E-5 72-73 0.004614348664268566 0.0 0.0 1.2002819244529037 8.166988786316047E-5 74-75 0.004655183608200147 0.0 0.0 1.3332813368380605 8.166988786316047E-5 76-77 0.004655183608200147 0.0 0.0 1.4822676297724309 8.166988786316047E-5 78-79 0.004655183608200147 0.0 0.0 1.6515897247847284 8.166988786316047E-5 80-81 0.00522687282324227 0.0 0.0 1.8465357471140924 8.166988786316047E-5 82-83 0.0054310475429001715 0.0 0.0 2.0548143786371176 8.166988786316047E-5 84-85 0.005573969846660702 0.0 0.0 2.261541282290742 8.166988786316047E-5 86-87 0.005737309622387023 0.0 0.0 2.4737604859031648 8.166988786316047E-5 88-89 0.005757727094352813 0.0 0.0 2.7208527316331566 8.166988786316047E-5 90-91 0.006023154229908085 0.0 0.0 3.0089432610704554 8.166988786316047E-5 92-93 0.006084406645805455 0.0 0.0 3.3171450003940572 8.166988786316047E-5 94-95 0.006084406645805455 0.0 0.0 3.625305904773727 8.166988786316047E-5 96-97 0.006084406645805455 0.0 0.0 3.9316904890923743 8.166988786316047E-5 98-99 0.006349833781360727 0.0 0.0 4.269170883214919 8.166988786316047E-5 100-101 0.006635678388881789 0.0 0.0 4.667393256435689 8.166988786316047E-5 102-103 0.00692152299640285 0.0 0.0 5.100059904862748 8.166988786316047E-5 104-105 0.007942396594692355 0.0 0.0 5.531317747724167 8.166988786316047E-5 106-107 0.008350746034008158 0.0 0.0 5.9471195643074815 8.166988786316047E-5 108-109 0.008452833393837109 0.0 0.0 6.3875040171376085 8.166988786316047E-5 110-111 0.00859575569759764 0.0 0.0 6.883138149107165 8.166988786316047E-5 112-113 0.0086774255854608 0.0 0.0 7.428100893346068 8.166988786316047E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4865 0.0 60.466354 1 GTATCAA 8310 0.0 49.54476 1 ATCAACG 10585 0.0 37.04836 3 TATCAAC 11140 0.0 36.48658 2 TCAACGC 10635 0.0 36.42586 4 CAACGCA 10885 0.0 35.525284 5 AACGCAG 11090 0.0 34.332153 6 GTACATG 10640 0.0 32.638565 1 TACATGG 10915 0.0 31.232464 2 CGCAGAG 13165 0.0 29.00002 8 ACGCAGA 13130 0.0 28.816751 7 ACATGGG 10940 0.0 28.437218 3 GCAGAGT 14030 0.0 25.940472 9 CATGGGA 6175 0.0 24.225388 4 GAGTACT 8800 0.0 23.889097 12-13 AGAGTAC 13795 0.0 23.688578 10-11 AGTACTT 9255 0.0 23.260826 12-13 GTACTTT 9290 0.0 23.01339 14-15 CAGAGTA 14440 0.0 22.67165 10-11 CATGGGG 5070 0.0 21.629368 4 >>END_MODULE