FastQCFastQC Report
Thu 26 May 2016
SRR1364515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692255
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34040.126436760262308No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.053.925751
GTATCAA69400.050.7792931
ATCAACG86900.038.8234373
GTACATG126050.038.7725831
TCAACGC91200.037.37964
CAACGCA92100.037.2070735
AACGCAG91050.036.634566
TACATGG131250.036.0858042
TATCAAC99300.035.7124442
ACATGGG132450.033.7379463
ACGCAGA106200.030.9620557
CGCAGAG105650.030.4483478
GCAGAGT113850.026.7902289
CATGGGA73200.025.483954
GTATAGG9950.025.127581
CATGGGG65700.024.8552784
GAGTACT49900.024.37710812-13
GAGTACA100900.024.0119631
AGTACAT93400.023.698382
CATGGGT27250.023.6205884
Produced by FastQC (version 0.11.5)