##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364515_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2692255 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.495300779458113 30.0 25.0 31.0 2.0 34.0 2 25.642962126544475 30.0 25.0 31.0 2.0 34.0 3 25.736792020072393 30.0 25.0 31.0 2.0 34.0 4 28.45330104317756 35.0 26.0 37.0 2.0 37.0 5 29.078593966767635 35.0 28.0 37.0 2.0 37.0 6 29.106444968994392 35.0 28.0 37.0 2.0 37.0 7 28.93016449036217 35.0 28.0 37.0 2.0 37.0 8 28.588418630478913 35.0 27.0 37.0 2.0 37.0 9 29.18156489634154 35.0 27.0 39.0 2.0 39.0 10-11 29.23437750138824 35.0 27.0 39.0 2.0 39.0 12-13 29.06470096629034 35.0 26.5 39.0 2.0 39.0 14-15 29.659871928922037 36.0 26.5 40.0 2.0 41.0 16-17 29.89499843068357 36.0 27.0 40.0 2.0 41.0 18-19 29.83966600489181 36.0 27.0 40.0 2.0 41.0 20-21 29.61953213941473 36.0 26.0 40.0 2.0 41.0 22-23 29.649200020057535 36.0 26.0 40.0 2.0 41.0 24-25 29.653241799160924 36.0 26.0 40.0 2.0 41.0 26-27 29.745065381993903 36.0 26.5 40.0 2.0 41.0 28-29 29.65040792941233 36.0 26.5 40.0 2.0 41.0 30-31 29.288931397657354 35.5 25.0 40.0 2.0 41.0 32-33 29.273230061788354 35.0 25.0 40.0 2.0 41.0 34-35 28.96272511333436 35.0 24.5 40.0 2.0 41.0 36-37 28.711142703792916 35.0 24.0 40.0 2.0 41.0 38-39 28.53990205236874 35.0 24.0 40.0 2.0 41.0 40-41 28.539342298556413 35.0 24.0 40.0 2.0 41.0 42-43 28.374130607984757 35.0 23.5 39.5 2.0 41.0 44-45 28.00748220358027 34.5 22.0 39.0 2.0 41.0 46-47 27.725913778598237 34.0 20.5 39.0 2.0 41.0 48-49 27.459410419889647 33.5 19.0 39.0 2.0 40.0 50-51 27.422851661525378 33.5 19.5 39.0 2.0 40.0 52-53 27.212357298992853 33.0 18.0 39.0 2.0 40.0 54-55 27.071645330772903 33.0 17.0 39.0 2.0 40.0 56-57 26.65142157782231 33.0 14.0 38.0 2.0 40.0 58-59 26.42449563655746 33.0 13.0 38.0 2.0 40.0 60-61 26.008593539616417 32.5 10.0 38.0 2.0 40.0 62-63 25.946877988897782 32.0 11.0 38.0 2.0 40.0 64-65 25.76837093811693 32.0 9.0 37.0 2.0 40.0 66-67 25.525597872415503 32.0 8.0 37.0 2.0 40.0 68-69 25.349661343372006 32.0 7.5 36.0 2.0 39.5 70-71 25.230724429892412 32.0 7.0 36.0 2.0 39.0 72-73 25.21373588311657 32.0 7.0 36.0 2.0 39.0 74-75 24.956975657952164 32.0 4.0 35.5 2.0 39.0 76-77 24.68790623473631 32.0 2.0 35.0 2.0 38.0 78-79 24.39163767919458 32.0 2.0 35.0 2.0 37.0 80-81 24.40543967789084 32.0 2.0 35.0 2.0 37.0 82-83 24.224940245259084 32.0 2.0 35.0 2.0 36.0 84-85 24.058864037767595 32.0 2.0 35.0 2.0 36.0 86-87 23.86617686660439 32.0 2.0 35.0 2.0 36.0 88-89 23.65255705718812 31.0 2.0 35.0 2.0 35.0 90-91 23.499404588346945 31.0 2.0 35.0 2.0 35.0 92-93 23.40205719740515 31.0 2.0 35.0 2.0 35.0 94-95 23.33939355670247 31.0 2.0 35.0 2.0 35.0 96-97 23.21445145426418 31.0 2.0 35.0 2.0 35.0 98-99 23.132723869024296 31.0 2.0 35.0 2.0 35.0 100-101 23.07924713669396 31.0 2.0 35.0 2.0 35.0 102-103 21.98890261137968 29.5 2.0 33.5 2.0 34.5 104-105 22.72749869533161 30.5 2.0 34.0 2.0 35.0 106-107 22.819674213623898 31.0 2.0 34.5 2.0 35.0 108-109 22.76723118723895 31.0 2.0 35.0 2.0 35.0 110-111 22.668120590360125 31.0 2.0 35.0 2.0 35.0 112-113 22.656697452507288 31.0 2.0 35.0 2.0 35.0 114-115 22.609080863439758 31.0 2.0 35.0 2.0 35.0 116-117 22.415695578613466 31.0 2.0 35.0 2.0 35.0 118-119 22.270891873169518 31.0 2.0 35.0 2.0 35.0 120-121 22.130043402278016 31.0 2.0 35.0 2.0 35.0 122-123 21.85868649143562 31.0 2.0 34.5 2.0 35.0 124-125 20.901539972996616 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.706331124711312 1101 2 1.3299993570806627 1101 3 1.6573098837452847 1101 4 2.0538484415188165 1101 5 1.4440953313371523 1101 6 1.4162765634109675 1101 7 1.4492846630329304 1101 8 1.7736718552042134 1101 9 2.223280295019112 1101 10-11 2.21665236473768 1101 12-13 2.0704442674564127 1101 14-15 2.305421821921417 1101 16-17 1.9802818582426198 1101 18-19 2.0336713840440126 1101 20-21 2.2976997972868993 1101 22-23 2.154409803868507 1101 24-25 2.1239555863729755 1101 26-27 1.8420504086553748 1101 28-29 1.7321972172065045 1101 30-31 1.946053403479251 1101 32-33 2.143343499792625 1101 34-35 2.375010102898802 1101 36-37 2.3407426613248994 1101 38-39 2.361591036696133 1101 40-41 2.0635455065369364 1101 42-43 2.0725765070070565 1101 44-45 2.291542909283372 1101 46-47 2.274076333504553 1101 48-49 2.309120877891907 1101 50-51 2.2167625455620197 1101 52-53 2.211074044199016 1101 54-55 2.2621271475437084 1101 56-57 2.4582230923523802 1101 58-59 2.4709250551400537 1101 60-61 2.578774243965846 1101 62-63 2.300622001088321 1101 64-65 2.1446864932850147 1101 66-67 2.318307882234887 1101 68-69 2.1149617199773196 1101 70-71 2.1262625584875643 1101 72-73 1.9938335513666061 1101 74-75 1.9276046552741626 1101 76-77 2.2039621184624814 1101 78-79 2.2205077390397285 1101 80-81 2.036252455454626 1101 82-83 2.1237543583086165 1101 84-85 1.9351284260076902 1101 86-87 1.8039663522608365 1101 88-89 2.0592160426322934 1101 90-91 2.0106891319704374 1101 92-93 1.9448314492581922 1101 94-95 1.8958155437715725 1101 96-97 1.9670112572743683 1101 98-99 1.8795999154565983 1101 100-101 1.910131386291745 1101 102-103 1.9257383208701455 1101 104-105 1.88495702410588 1101 106-107 2.0482221036539165 1101 108-109 2.098527938531003 1101 110-111 2.250858729087504 1101 112-113 2.0806843665668247 1101 114-115 2.0870749811375866 1101 116-117 2.292498756609959 1101 118-119 2.223506366552261 1101 120-121 2.192744131138447 1101 122-123 2.1407097288415464 1101 124-125 2.0577328580749032 1102 1 0.9047887405615 1102 2 0.5722921951905846 1102 3 0.7877749661576274 1102 4 1.1524014914686163 1102 5 1.0220611485990716 1102 6 1.0613536201401885 1102 7 0.9223449667100283 1102 8 1.217038805740227 1102 9 1.591405772875703 1102 10-11 1.1870832090573593 1102 12-13 1.4676659054451626 1102 14-15 1.7418979738484914 1102 16-17 1.223229579578316 1102 18-19 1.4629362880604546 1102 20-21 1.57240183724333 1102 22-23 1.5234510555266603 1102 24-25 1.2853021893125849 1102 26-27 1.3285108709455358 1102 28-29 1.0025946502487777 1102 30-31 0.9671664170958962 1102 32-33 0.8990250810931499 1102 34-35 1.2803324283003512 1102 36-37 1.4932323854010043 1102 38-39 1.568222299398343 1102 40-41 1.1481656392314399 1102 42-43 1.2514411705463857 1102 44-45 1.540955321578771 1102 46-47 1.4308692923084863 1102 48-49 1.524038306725359 1102 50-51 1.4598189031815743 1102 52-53 1.4937022411450087 1102 54-55 1.324312851183926 1102 56-57 1.4369938516338792 1102 58-59 1.4712119880606949 1102 60-61 1.8667823747829644 1102 62-63 1.6928642960285032 1102 64-65 1.7548924415200773 1102 66-67 1.7663449083766132 1102 68-69 1.533358324335829 1102 70-71 1.6516572313916242 1102 72-73 1.5830086127967604 1102 74-75 1.6227627761006005 1102 76-77 1.577735894802185 1102 78-79 1.5598274639438152 1102 80-81 1.0642498738758164 1102 82-83 1.060818185526653 1102 84-85 1.203827968234652 1102 86-87 1.352018916223109 1102 88-89 1.1069093273392347 1102 90-91 0.994382244774549 1102 92-93 0.9476473507840275 1102 94-95 1.128176993533728 1102 96-97 1.2409664191298795 1102 98-99 1.3211389094303065 1102 100-101 1.2155199795344913 1102 102-103 1.068476121053422 1102 104-105 1.0947223019993046 1102 106-107 1.3767363575167977 1102 108-109 1.4483974406912914 1102 110-111 1.2693467502511737 1102 112-113 1.1996858832122612 1102 114-115 1.3021747647759163 1102 116-117 1.480391530349742 1102 118-119 1.627898320425313 1102 120-121 1.5466394528238894 1102 122-123 1.6340712081495 1102 124-125 1.6205565115776501 1103 1 1.3930401523805926 1103 2 1.0112248344037305 1103 3 1.272829545655064 1103 4 1.7861018629204857 1103 5 1.2527420507694735 1103 6 1.3848028260137255 1103 7 1.2774867526060092 1103 8 1.5518889589437954 1103 9 2.158915117248057 1103 10-11 1.6601565493133101 1103 12-13 1.751667805680487 1103 14-15 2.1192861027206824 1103 16-17 1.9016796217412413 1103 18-19 1.5092365205953655 1103 20-21 2.0181825830740756 1103 22-23 1.8618712351895006 1103 24-25 1.8183618120996101 1103 26-27 1.598296606636275 1103 28-29 1.3700487066007447 1103 30-31 1.7411108340511952 1103 32-33 1.7021274243295395 1103 34-35 1.4894675540805977 1103 36-37 1.8002456591719458 1103 38-39 1.878550008685572 1103 40-41 2.0609124317131986 1103 42-43 2.0646448441798064 1103 44-45 1.8392498139162683 1103 46-47 1.9345218442687724 1103 48-49 1.9780494758346876 1103 50-51 1.796173912671133 1103 52-53 1.8428577050184707 1103 54-55 1.733349422583295 1103 56-57 1.9992061158176924 1103 58-59 2.053122990609058 1103 60-61 2.064850488230263 1103 62-63 1.8306253751205759 1103 64-65 1.6504468084606252 1103 66-67 1.8283702270377127 1103 68-69 1.6902474323635062 1103 70-71 1.8733326907119547 1103 72-73 1.8533938634396563 1103 74-75 1.833202861773799 1103 76-77 1.8446828926394474 1103 78-79 1.99330273416982 1103 80-81 1.5597984913268519 1103 82-83 1.672945310805126 1103 84-85 1.7346909375730277 1103 86-87 1.8099363117392073 1103 88-89 1.8790615296840976 1103 90-91 1.8678872613493382 1103 92-93 1.7590522472583707 1103 94-95 1.571786755353866 1103 96-97 1.8425655902741802 1103 98-99 1.682859679374065 1103 100-101 1.5309851771194758 1103 102-103 1.649235657656181 1103 104-105 1.7645085603709063 1103 106-107 1.5969091674258955 1103 108-109 1.8864839176596497 1103 110-111 1.9663531827103498 1103 112-113 1.8309430700741594 1103 114-115 1.7856653669739622 1103 116-117 1.8964735494310787 1103 118-119 1.953420652836403 1103 120-121 1.9384673374995813 1103 122-123 1.9592009491553277 1103 124-125 1.8719161206588204 1104 1 1.0990706105939196 1104 2 0.9588345764623831 1104 3 0.8116628888745758 1104 4 1.1702121399311665 1104 5 0.9065263695349763 1104 6 0.7233823141733815 1104 7 0.7580699711498404 1104 8 1.15749497061827 1104 9 1.5483170254999408 1104 10-11 1.3181572127856427 1104 12-13 0.7044848169353486 1104 14-15 1.0980993660159442 1104 16-17 0.8323572532768253 1104 18-19 0.3054011232479361 1104 20-21 -3.930418687629224 1104 22-23 -0.986583555322408 1104 24-25 0.38654748988492926 1104 26-27 0.6838036357082267 1104 28-29 0.34464815165666707 1104 30-31 1.0274195222433669 1104 32-33 0.9783419961152724 1104 34-35 1.1224291122299945 1104 36-37 1.2685758948364416 1104 38-39 1.2876105801944888 1104 40-41 1.2941560205273213 1104 42-43 1.2712531494473822 1104 44-45 1.5202062356474073 1104 46-47 1.374144708579891 1104 48-49 1.4940665108163778 1104 50-51 1.3062655026100813 1104 52-53 1.276339660548249 1104 54-55 1.3169180469327273 1104 56-57 1.5477914038679401 1104 58-59 1.5493012691400132 1104 60-61 1.6618990798894089 1104 62-63 1.2560616850719981 1104 64-65 1.4091871227531811 1104 66-67 1.4426443839115741 1104 68-69 1.3977996478558055 1104 70-71 1.5512864058274793 1104 72-73 1.3812254293442088 1104 74-75 1.4704154760964556 1104 76-77 1.4392615877080033 1104 78-79 1.3896339738305272 1104 80-81 1.1569349051929834 1104 82-83 1.098347733138219 1104 84-85 1.1020481537134224 1104 86-87 0.9821721366088738 1104 88-89 1.2198897386822765 1104 90-91 1.1647965677571186 1104 92-93 0.834151932877937 1104 94-95 1.060899492924193 1104 96-97 1.116996750625777 1104 98-99 0.9970573831446536 1104 100-101 0.43498820009275363 1104 102-103 0.881417251351877 1104 104-105 1.050257965552582 1104 106-107 1.1556568963513136 1104 108-109 1.2070518895213915 1104 110-111 1.2385572070435273 1104 112-113 1.156494389219727 1104 114-115 1.0257620537864618 1104 116-117 1.129033179556238 1104 118-119 1.0758449741503426 1104 120-121 1.0991742321195233 1104 122-123 0.8086624271168397 1104 124-125 0.9795102974807897 1105 1 1.0726844958034896 1105 2 0.7804281121233672 1105 3 0.8189875792010319 1105 4 1.3476364276205643 1105 5 0.9849893284091245 1105 6 0.9394134523843505 1105 7 0.8331552167748342 1105 8 1.1678337363001567 1105 9 1.6022196994343432 1105 10-11 1.267434211182934 1105 12-13 1.3092440884282404 1105 14-15 1.5048376514832071 1105 16-17 1.375154188974264 1105 18-19 1.0042933896532205 1105 20-21 1.2301179196558003 1105 22-23 1.2056285257382804 1105 24-25 1.19861889490247 1105 26-27 1.063452116124818 1105 28-29 0.7067417470012138 1105 30-31 1.2072687453936801 1105 32-33 1.1182183459437844 1105 34-35 0.9341730881209322 1105 36-37 1.2576204728116345 1105 38-39 1.1788331874870437 1105 40-41 1.3027313528119642 1105 42-43 1.56634363925345 1105 44-45 1.726108995508426 1105 46-47 1.9777536042877912 1105 48-49 1.5692446036928622 1105 50-51 1.6405138916521373 1105 52-53 1.5689941418759297 1105 54-55 1.6414716216238858 1105 56-57 1.941396979885205 1105 58-59 1.647206508914028 1105 60-61 1.9828460066952118 1105 62-63 1.882841202027116 1105 64-65 1.6574086854172698 1105 66-67 1.863123398218132 1105 68-69 1.4122632232755663 1105 70-71 1.4102889129800928 1105 72-73 1.3974684386714529 1105 74-75 1.760689369398186 1105 76-77 1.5915763134260352 1105 78-79 1.4497750750775502 1105 80-81 1.4276661960565704 1105 82-83 1.4882928464052227 1105 84-85 1.4196702962935817 1105 86-87 1.4894099591132743 1105 88-89 1.5747414698710465 1105 90-91 1.552075741337962 1105 92-93 1.5803086546092437 1105 94-95 1.4190772263705291 1105 96-97 1.5113128268090215 1105 98-99 1.3343546168169915 1105 100-101 1.4173670430111471 1105 102-103 1.4993860790360145 1105 104-105 1.5462176983030886 1105 106-107 1.6725973396839287 1105 108-109 1.6392786002527657 1105 110-111 1.5943938831362843 1105 112-113 1.598152597946548 1105 114-115 1.6470356235346237 1105 116-117 1.7908242489904822 1105 118-119 1.7969874725907111 1105 120-121 1.8015211692453796 1105 122-123 1.8814451494890498 1105 124-125 1.5381632405448862 1106 1 -0.24684272853621536 1106 2 -0.015140948693741052 1106 3 0.11910417395725759 1106 4 -0.37638197934646556 1106 5 -0.888153940993579 1106 6 -0.262613615423934 1106 7 -0.13780056126530127 1106 8 0.1372557884468364 1106 9 0.3657813843780531 1106 10-11 -2.1701203844385866 1106 12-13 -0.3419826948595066 1106 14-15 -0.03844610698570605 1106 16-17 -0.16723835573026236 1106 18-19 -3.0920488235713464 1106 20-21 -5.587590973641664 1106 22-23 -6.381663153502608 1106 24-25 -6.161370235329983 1106 26-27 -5.2446321818275266 1106 28-29 -1.9600802195185487 1106 30-31 -3.7727648115586554 1106 32-33 -4.2466665512841715 1106 34-35 -3.5072641072421256 1106 36-37 -5.7663442058025645 1106 38-39 -6.229514308884532 1106 40-41 -2.6097652697758313 1106 42-43 -1.2984124227431444 1106 44-45 -0.6074384803307353 1106 46-47 -0.3397694537777518 1106 48-49 -0.2199531205941021 1106 50-51 -0.2760216364679593 1106 52-53 -0.3961108300696914 1106 54-55 -0.4214981771190125 1106 56-57 -0.3245377778182572 1106 58-59 -0.17995699506581886 1106 60-61 0.016492421674392688 1106 62-63 -0.19287132618379843 1106 64-65 -0.22094169338877023 1106 66-67 -0.5423943484965399 1106 68-69 -0.47869656535342386 1106 70-71 -0.21952992798792792 1106 72-73 -0.1423900174379824 1106 74-75 -0.2563942161246615 1106 76-77 -0.2926048185302328 1106 78-79 -0.2791863121462157 1106 80-81 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2216 84-85 -4.124614009104832 2216 86-87 -4.452036296537386 2216 88-89 -4.674786836450902 2216 90-91 -4.771242328552098 2216 92-93 -4.467255932948699 2216 94-95 -7.5769625839490615 2216 96-97 -9.557291388241506 2216 98-99 -6.407334798752974 2216 100-101 -5.45953187663795 2216 102-103 -6.365130228610646 2216 104-105 -9.230743748182398 2216 106-107 -5.797687107797184 2216 108-109 -8.039043862709095 2216 110-111 -5.531991188150577 2216 112-113 -5.030162081808442 2216 114-115 -5.360774641731691 2216 116-117 -4.964908317932039 2216 118-119 -5.291687856941913 2216 120-121 -6.06803625358334 2216 122-123 -8.722483750655016 2216 124-125 -7.294616074720963 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 305704.0 3 44403.0 4 25751.0 5 21720.0 6 22238.0 7 23980.0 8 26322.0 9 27399.0 10 28339.0 11 28678.0 12 29267.0 13 29416.0 14 29481.0 15 24857.0 16 20141.0 17 17878.0 18 16464.0 19 15864.0 20 15798.0 21 17388.0 22 21175.0 23 29038.0 24 38157.0 25 48507.0 26 58051.0 27 65861.0 28 74818.0 29 84308.0 30 93725.0 31 105448.0 32 124104.0 33 150019.0 34 183458.0 35 226285.0 36 280940.0 37 267710.0 38 69389.0 39 174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10489068217166 18.5363461376152 13.554087069810745 23.804676110402397 2 15.469523550490392 20.207971987022 39.798329744021906 24.52417471846571 3 18.718566728279036 25.559546610241924 28.670302465461027 27.051584196018013 4 11.159040087528295 16.40523757547513 37.5503523690281 34.88536996796848 5 13.625992909659669 38.44266721373559 33.377596858516156 14.55374301808858 6 31.542705761828948 36.58641406213435 17.631899370246856 14.238980805789836 7 28.95590578673868 32.47415672742265 20.587105664904783 17.982831820933885 8 26.3206475934214 35.02139341939237 19.875246222117887 18.78271276506835 9 26.603014743110887 16.46058356553049 20.058305290751946 36.87809640060668 10-11 24.898341707524803 26.855369533305694 26.95336216183669 21.29292659733281 12-13 25.56872276462357 24.352545150686783 27.51651999356622 22.562212091123428 14-15 22.800548462844095 25.769915304765767 25.17150608476507 26.258030147625067 16-17 22.73744155205566 29.091718341234756 25.422810074808538 22.748030031901045 18-19 22.648551842563162 27.43196584838748 27.73237699109225 22.187105317957105 20-21 23.51525942302335 26.694794058322525 27.618564450433514 22.17138206822061 22-23 23.153712524974683 26.55628767564009 27.39324136260526 22.89675843677997 24-25 22.702585629628558 26.88388712085286 27.546775249031167 22.86675200048741 26-27 22.68050236597293 27.144136850318617 27.306134070623134 22.869226713085318 28-29 22.59128141761424 26.994497419394257 27.53194020802715 22.882280954964347 30-31 22.695096314728218 26.799673648530316 27.610129227530233 22.89510080921124 32-33 22.541096872499587 26.98227821581588 27.2809349292399 23.19568998244463 34-35 22.94806075813178 26.765561228091162 27.49308806778709 22.793289945989965 36-37 23.040452572557772 26.60893741654022 27.309228817698767 23.04138119320324 38-39 22.80267322100248 26.74225847092368 27.41973919731675 23.035329110757093 40-41 22.896427262992294 26.731098350775788 27.54378325033831 22.828691135893607 42-43 22.86874636859554 26.813844734147757 27.149457091102004 23.1679518061547 44-45 22.809648266395115 26.862550821180285 27.15362159535045 23.17417931707415 46-47 22.87625582047123 27.051932597963425 26.847762232995663 23.224049348569682 48-49 23.080466414036255 27.06034265955854 26.66423471819426 23.194956208210947 50-51 23.059653254776382 27.28809498159286 26.392497464606652 23.259754299024106 52-53 23.31942657418774 27.036210882712357 26.504663231490984 23.139699311608922 54-55 23.152679897158638 26.982762815885952 26.52225387670404 23.342303410251372 56-57 22.94890937183733 27.241512461045016 26.411587156922632 23.39799101019502 58-59 23.058958143906498 26.81518659104144 26.538158265015838 23.58769700003622 60-61 23.12458710374035 26.85267341271345 26.84098784477999 23.18175163876621 62-63 23.028890426779505 27.064924615499486 26.589255790956834 23.316929166764176 64-65 22.87380486584464 26.845169008504616 26.642792308125212 23.638233817525535 66-67 22.945336132284233 27.174037811097563 26.440149021475424 23.44047703514278 68-69 22.99205180692167 26.908885991822796 26.612934531279862 23.486127669975673 70-71 22.788288650541872 26.72303268834275 27.05043429745443 23.43824436366095 72-73 23.049297988897305 26.945028274892614 26.622210327908526 23.38346340830156 74-75 22.954421443506057 27.08870299445548 26.468450257762306 23.488425304276152 76-77 23.048082364821806 26.890020689274728 26.682143534420554 23.37975341148291 78-79 23.05363993499811 26.836463177855368 26.729092655930604 23.38080423121592 80-81 22.93875942414863 27.25846190762561 26.489170563485803 23.31360810473996 82-83 23.213633343864124 27.100498104550507 26.366165813338295 23.319702738247074 84-85 23.03191760046149 26.93480046252978 26.37041904410265 23.66286289290608 86-87 22.96930811199534 26.92540280642113 26.545162311226278 23.56012677035725 88-89 23.01961891424549 26.89646718386951 26.486054282153532 23.59785961973147 90-91 23.00470416577207 26.9951658233293 26.542189279524177 23.457940731374453 92-93 23.100052912892824 26.85157515683036 26.467237001657217 23.581134928619598 94-95 22.978240354754337 26.79284124591191 26.520490476545127 23.70842792278863 96-97 23.08991787760275 26.752608739094892 26.45576983293595 23.70170355036641 98-99 22.83502457794413 26.74747446232091 26.498491261350825 23.919009698384137 100-101 22.929963395515827 26.79872065386986 26.515899057131335 23.755416893482977 102-103 23.019803037509494 26.47932194714547 26.815863453405036 23.68501156194 104-105 22.870402648901827 26.6601092924592 26.70161910558332 23.76786895305565 106-107 22.804976910426568 26.7800979298779 26.731086561060387 23.68383859863515 108-109 22.900362712487674 26.681691469663456 26.470787065668976 23.9471587521799 110-111 22.718380823595417 26.863547748509713 26.633047115844814 23.78502431205005 112-113 22.77453611347346 26.85282984385717 26.724529399430224 23.648104643239144 114-115 22.621251539649027 26.98900738882954 26.608088190501945 23.78165288101949 116-117 22.592428187764895 27.065328425126943 26.673885345586513 23.668358041521643 118-119 22.681318983378983 26.87516668932527 26.460347070391 23.983167256904743 120-121 22.58150672400872 26.972928447186277 26.62796604424352 23.81759878456148 122-123 22.68146872537938 26.886787021150376 26.689803473344075 23.74194078012617 124-125 22.837612390044228 26.950680128633707 26.266574895168283 23.94513258615378 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1569.0 1 1626.5 2 1999.0 3 2069.5 4 1498.5 5 1031.0 6 880.0 7 967.5 8 1258.5 9 1419.5 10 1324.0 11 1269.0 12 1299.0 13 1377.5 14 1560.0 15 1748.5 16 1934.5 17 2148.0 18 2457.0 19 2732.0 20 3071.0 21 3795.5 22 4686.0 23 5857.5 24 7371.5 25 9445.5 26 12454.5 27 15934.5 28 19235.0 29 23032.0 30 28032.5 31 33687.5 32 39401.0 33 44891.5 34 50730.5 35 56150.0 36 61765.5 37 67194.5 38 69839.5 39 71647.5 40 74055.0 41 77158.0 42 80419.0 43 85250.0 44 89936.0 45 92354.5 46 93351.5 47 93971.0 48 96764.0 49 98832.5 50 97929.5 51 95758.5 52 94136.5 53 93556.0 54 91360.0 55 86248.0 56 80262.5 57 74788.0 58 70538.0 59 64554.5 60 56556.5 61 48086.0 62 39324.5 63 31001.5 64 24335.0 65 20036.5 66 16250.5 67 12898.5 68 10232.5 69 8070.5 70 6585.0 71 5524.5 72 4750.0 73 4202.5 74 3470.0 75 2761.5 76 2108.0 77 1458.0 78 1003.5 79 725.5 80 576.5 81 439.0 82 292.0 83 190.5 84 123.5 85 97.0 86 79.0 87 60.0 88 48.0 89 33.5 90 23.5 91 20.0 92 14.0 93 9.5 94 7.5 95 4.0 96 3.5 97 4.5 98 4.5 99 5.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10073340006797277 2 0.034580676793245814 3 0.04594661352657902 4 0.05452678145272271 5 0.057646842516774974 6 0.003305778984531554 7 0.0024143329662309103 8 0.0026743380549019316 9 0.008097301332897516 10-11 0.06487126962341977 12-13 0.007948726996514074 14-15 0.01366883894727654 16-17 0.024161901454357038 18-19 0.02267615809052263 20-21 0.13572265628627303 22-23 0.019648955986710025 24-25 0.017587487069389784 26-27 0.008673026886383347 28-29 0.005738683742810394 30-31 0.002432904758278841 32-33 0.0074658604032678925 34-35 0.019797530323093466 36-37 0.0033429225686274146 38-39 0.011013072684422538 40-41 0.03450638962505409 42-43 0.017048905099999814 44-45 0.11315792894803797 46-47 0.005218673565468353 48-49 0.011588798237908371 50-51 0.013557408194988959 52-53 0.014801718262200274 54-55 0.012703105760784174 56-57 0.007633006531699263 58-59 0.017550343485293926 60-61 0.03346636927037001 62-63 0.009508757528540203 64-65 0.00911874989553367 66-67 0.010920213724182889 68-69 0.009638760072875712 70-71 0.005515822238235235 72-73 9.471613944444341E-4 74-75 0.0017086048684095674 76-77 0.010418775338888776 78-79 0.023809037405446362 80-81 0.0037886455777777365 82-83 0.005552965822331094 84-85 0.0038629327459694567 86-87 0.005311532525708003 88-89 0.0078001526601306345 90-91 0.0064258400485838075 92-93 0.003510068697058785 94-95 0.0051443863972766325 96-97 0.0020057535411764486 98-99 0.003918648122113247 100-101 0.0036586430334422256 102-103 6.128691375816927E-4 104-105 0.0044758018835511495 106-107 8.357306421568536E-4 108-109 0.0014857433638344065 110-111 0.0026557662628540015 112-113 0.005070099229084911 114-115 0.0030829174799563933 116-117 0.0061286913758169265 118-119 0.00449437367559908 120-121 0.008264447461328886 122-123 0.005645824782570745 124-125 0.056235386321132286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2692255.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.30113788984682 #Duplication Level Percentage of deduplicated Percentage of total 1 81.58492628157538 50.0124881571984 2 9.789881012690465 12.002616917682628 3 3.509614908287052 6.454301622995 4 1.670156888584591 4.0953007089920614 5 0.9433307271826656 2.8913623491377027 6 0.5625191245282274 2.068983745102847 7 0.3742750220017774 1.6060439312719488 8 0.2606612107710213 1.278306305920704 9 0.20596766715420936 1.1363447128563235 >10 0.9904549249800255 10.853082337519192 >50 0.07168905211165105 3.0335657208726055 >100 0.03512445106227452 3.782230322782075 >500 0.0011444146942101033 0.42665277487830744 >1k 2.5431437648299246E-4 0.3587203927901514 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3404 0.126436760262308 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.714358409586016E-5 0.0 10-11 0.0 0.0 0.0 7.428716819172032E-5 0.0 12-13 0.0 0.0 0.0 7.428716819172032E-5 0.0 14-15 0.0 0.0 0.0 9.28589602396504E-5 0.0 16-17 0.0 0.0 0.0 1.1143075228758049E-4 0.0 18-19 0.0 0.0 0.0 1.4857433638344064E-4 0.0 20-21 0.0 0.0 0.0 2.2286150457516098E-4 0.0 22-23 3.714358409586016E-5 0.0 0.0 4.0857942505446174E-4 0.0 24-25 3.714358409586016E-5 0.0 0.0 7.800152660130634E-4 0.0 26-27 3.714358409586016E-5 0.0 0.0 0.001151451106971665 0.0 28-29 3.714358409586016E-5 0.0 0.0 0.002005753541176449 0.0 30-31 3.714358409586016E-5 3.714358409586016E-5 0.0 0.005032955644989052 0.0 32-33 3.714358409586016E-5 3.714358409586016E-5 0.0 0.011663085406100091 0.0 34-35 3.714358409586016E-5 3.714358409586016E-5 0.0 0.019296091937799353 0.0 36-37 3.714358409586016E-5 5.571537614379024E-5 0.0 0.0314234721450977 0.0 38-39 3.714358409586016E-5 7.428716819172032E-5 0.0 0.05361676364237414 0.0 40-41 3.714358409586016E-5 7.428716819172032E-5 0.0 0.08101015691307101 0.0 42-43 3.714358409586016E-5 7.428716819172032E-5 0.0 0.1088492731929182 0.0 44-45 3.714358409586016E-5 7.428716819172032E-5 0.0 0.14053275042668695 0.0 46-47 3.714358409586016E-5 7.428716819172032E-5 0.0 0.1781034857396495 0.0 48-49 3.714358409586016E-5 7.428716819172032E-5 0.0 0.2210414689544638 0.0 50-51 3.714358409586016E-5 7.428716819172032E-5 0.0 0.27122245106797094 0.0 52-53 3.714358409586016E-5 7.428716819172032E-5 0.0 0.3198991180255956 0.0 54-55 3.714358409586016E-5 9.28589602396504E-5 0.0 0.3726244356496692 0.0 56-57 3.714358409586016E-5 1.1143075228758049E-4 0.0 0.433762775071455 0.0 58-59 3.714358409586016E-5 1.1143075228758049E-4 0.0 0.5050970283275544 0.0 60-61 3.714358409586016E-5 1.1143075228758049E-4 0.0 0.5879457926533704 0.0 62-63 3.714358409586016E-5 1.1143075228758049E-4 0.0 0.6723731593032607 0.0 64-65 3.714358409586016E-5 1.1143075228758049E-4 0.0 0.7570419592497739 0.0 66-67 3.714358409586016E-5 1.1143075228758049E-4 0.0 0.8561781852016247 0.0 68-69 3.714358409586016E-5 1.1143075228758049E-4 0.0 0.9725861777580505 0.0 70-71 3.714358409586016E-5 1.1143075228758049E-4 0.0 1.1069716650168724 0.0 72-73 3.714358409586016E-5 1.1143075228758049E-4 0.0 1.2443100672113154 0.0 74-75 3.714358409586016E-5 1.1143075228758049E-4 0.0 1.3867742840109871 0.0 76-77 3.714358409586016E-5 1.1143075228758049E-4 0.0 1.5431859166386541 0.0 78-79 3.714358409586016E-5 1.1143075228758049E-4 0.0 1.7273623783779768 0.0 80-81 3.714358409586016E-5 1.1143075228758049E-4 0.0 1.9378922130333123 0.0 82-83 3.714358409586016E-5 1.1143075228758049E-4 0.0 2.1531764264529176 0.0 84-85 3.714358409586016E-5 1.1143075228758049E-4 0.0 2.374923623505203 0.0 86-87 3.714358409586016E-5 1.1143075228758049E-4 0.0 2.611936833620887 0.0 88-89 3.714358409586016E-5 1.1143075228758049E-4 0.0 2.8698247379984436 0.0 90-91 3.714358409586016E-5 1.1143075228758049E-4 0.0 3.1632033369795955 0.0 92-93 3.714358409586016E-5 1.1143075228758049E-4 0.0 3.4873925389682627 0.0 94-95 3.714358409586016E-5 1.1143075228758049E-4 0.0 3.8089074029020282 0.0 96-97 3.714358409586016E-5 1.1143075228758049E-4 0.0 4.128509372254857 0.0 98-99 3.714358409586016E-5 1.1143075228758049E-4 0.0 4.475653309214765 0.0 100-101 3.714358409586016E-5 1.1143075228758049E-4 0.0 4.877194025083062 0.0 102-103 3.714358409586016E-5 1.1143075228758049E-4 0.0 5.303899519176303 0.0 104-105 5.571537614379024E-5 1.1143075228758049E-4 0.0 5.736492271348739 0.0 106-107 7.428716819172032E-5 1.1143075228758049E-4 0.0 6.173412251068342 0.0 108-109 7.428716819172032E-5 1.1143075228758049E-4 0.0 6.624781085001235 0.0 110-111 7.428716819172032E-5 1.1143075228758049E-4 0.0 7.126312329255587 0.0 112-113 7.428716819172032E-5 1.3000254433551058E-4 0.0 7.662981404064622 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4250 0.0 53.92575 1 GTATCAA 6940 0.0 50.779293 1 ATCAACG 8690 0.0 38.823437 3 GTACATG 12605 0.0 38.772583 1 TCAACGC 9120 0.0 37.3796 4 CAACGCA 9210 0.0 37.207073 5 AACGCAG 9105 0.0 36.63456 6 TACATGG 13125 0.0 36.085804 2 TATCAAC 9930 0.0 35.712444 2 ACATGGG 13245 0.0 33.737946 3 ACGCAGA 10620 0.0 30.962055 7 CGCAGAG 10565 0.0 30.448347 8 GCAGAGT 11385 0.0 26.790228 9 CATGGGA 7320 0.0 25.48395 4 GTATAGG 995 0.0 25.12758 1 CATGGGG 6570 0.0 24.855278 4 GAGTACT 4990 0.0 24.377108 12-13 GAGTACA 10090 0.0 24.011963 1 AGTACAT 9340 0.0 23.69838 2 CATGGGT 2725 0.0 23.620588 4 >>END_MODULE