FastQCFastQC Report
Thu 26 May 2016
SRR1364515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692255
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70090.2603393809278839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64700.24031898910021524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41540.1542944483342031No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55800.060.8598141
GTATCAA95250.051.6315041
ATCAACG128850.036.2681963
TATCAAC138600.035.3941082
TCAACGC131100.035.3729484
CAACGCA130950.035.352925
AACGCAG132450.034.5482146
GTACATG125600.034.260851
TACATGG130300.032.1134532
ACATGGG131300.030.0529823
CGCAGAG158550.029.2939418
ACGCAGA156850.028.908247
CATGGGG66250.026.7217484
GCAGAGT168700.025.9098749
GAGTACT96500.025.26723112-13
AGTACTT105200.024.25172612-13
GTGGTAT30450.023.9125061
AGAGTAC161950.023.70371410-11
GTACTTT106100.023.37339214-15
CAGAGTA165500.023.1054310-11
Produced by FastQC (version 0.11.5)