FastQCFastQC Report
Thu 26 May 2016
SRR1364514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088587
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35450.11477740468375992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88800.051.282721
GGTATCA49300.050.3514181
ATCAACG106250.041.4961433
TCAACGC108100.040.5495384
CAACGCA110750.039.739965
GTACATG152600.039.2043341
TATCAAC116700.039.1059652
AACGCAG110150.038.8542756
TACATGG157150.035.968952
ACATGGG156250.033.129723
ACGCAGA128150.032.93397
CGCAGAG126850.032.663488
CATGGGA107600.029.280414
GCAGAGT136100.028.4323659
CATGGGG59800.024.6992784
AGAGTAC127100.023.73762110-11
GAGTACT56200.023.2849212-13
ATGGGAG50450.022.9016765
CAGAGTA137400.022.6727810-11
ATGGGAT49000.022.485495
Produced by FastQC (version 0.11.5)