##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364514_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3088587 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.517160112375013 31.0 26.0 31.0 10.0 34.0 2 26.694708615946386 31.0 26.0 31.0 10.0 34.0 3 26.780454298357146 31.0 26.0 31.0 10.0 34.0 4 29.583814864208133 35.0 28.0 37.0 10.0 37.0 5 30.207625687733582 35.0 30.0 37.0 10.0 37.0 6 30.312749163290526 35.0 30.0 37.0 10.0 37.0 7 30.156493244321755 35.0 30.0 37.0 10.0 37.0 8 29.780175206332217 35.0 30.0 37.0 8.0 37.0 9 30.396906740849456 35.0 28.0 39.0 8.0 39.0 10-11 30.517944775394056 35.0 28.5 39.0 7.5 39.0 12-13 30.34524347217676 35.0 27.5 39.0 7.0 39.0 14-15 31.032822938126724 36.0 27.5 40.0 2.0 41.0 16-17 31.30333239763037 37.0 29.0 40.0 2.0 41.0 18-19 31.251878286090047 37.0 29.0 40.0 2.0 41.0 20-21 31.03006973739124 37.0 27.5 40.0 2.0 41.0 22-23 31.1003798824511 37.0 28.0 40.0 2.0 41.0 24-25 31.13100229975714 37.0 28.5 40.0 2.0 41.0 26-27 31.253423976724633 37.0 29.0 40.0 2.0 41.0 28-29 31.214121214652526 37.0 29.0 40.0 2.0 41.0 30-31 30.86192375348339 36.0 28.5 40.0 2.0 41.0 32-33 30.885353075694482 36.0 29.0 40.0 2.0 41.0 34-35 30.595502571240505 36.0 28.0 40.0 2.0 41.0 36-37 30.35679341394625 36.0 27.5 40.0 2.0 41.0 38-39 30.21389586888762 36.0 27.0 40.0 2.0 41.0 40-41 30.24678469474876 36.0 27.5 40.0 2.0 41.0 42-43 30.119761560869097 36.0 27.0 40.0 2.0 41.0 44-45 29.76993557248023 35.0 26.5 40.0 2.0 41.0 46-47 29.522237676970086 35.0 26.0 40.0 2.0 41.0 48-49 29.27614116099045 35.0 25.5 40.0 2.0 41.0 50-51 29.26439274658606 35.0 26.0 40.0 2.0 41.0 52-53 29.08348348290011 35.0 25.5 39.5 2.0 41.0 54-55 28.966943459905778 35.0 25.5 39.0 2.0 41.0 56-57 28.564579854800918 34.0 24.0 39.0 2.0 41.0 58-59 28.326394075996564 34.0 23.5 39.0 2.0 40.0 60-61 27.926881936626685 34.0 22.5 39.0 2.0 40.0 62-63 27.88191720032494 34.0 23.0 38.0 2.0 40.0 64-65 27.705379191196492 33.5 22.5 38.0 2.0 40.0 66-67 27.464846222560674 33.0 22.0 38.0 2.0 40.0 68-69 27.2912132635409 33.0 22.5 37.0 2.0 40.0 70-71 27.15152754317751 33.0 22.0 37.0 2.0 40.0 72-73 27.1328714392698 33.0 22.5 37.0 2.0 39.5 74-75 26.851385277474783 33.0 22.0 36.0 2.0 39.0 76-77 26.54537948906733 33.0 22.0 36.0 2.0 39.0 78-79 26.234091673635874 33.0 20.5 35.0 2.0 38.0 80-81 26.220743498564232 33.0 22.0 35.0 2.0 37.0 82-83 26.01261547756304 33.0 21.0 35.0 2.0 37.0 84-85 25.818789789635197 33.0 20.0 35.0 2.0 36.0 86-87 25.597108321701803 33.0 20.0 35.0 2.0 36.0 88-89 25.36548411943714 32.0 19.5 35.0 2.0 36.0 90-91 25.199637245122123 32.0 18.5 35.0 2.0 35.5 92-93 25.0863950084618 32.0 18.0 35.0 2.0 35.0 94-95 25.025866358953138 32.0 18.0 35.0 2.0 35.0 96-97 24.903248475759305 32.0 16.5 35.0 2.0 35.0 98-99 24.811583096088924 32.0 13.5 35.0 2.0 35.0 100-101 24.750437012135322 32.0 12.0 35.0 2.0 35.0 102-103 23.59217839743546 30.0 7.5 34.0 2.0 35.0 104-105 24.394497710441698 32.0 7.5 34.5 2.0 35.0 106-107 24.48136736960947 32.0 7.0 35.0 2.0 35.0 108-109 24.437084336623833 32.0 6.0 35.0 2.0 35.0 110-111 24.336573811908163 32.0 2.0 35.0 2.0 35.0 112-113 24.3256124888177 32.0 2.0 35.0 2.0 35.0 114-115 24.27996507788189 32.0 2.0 35.0 2.0 35.0 116-117 24.091600301367585 32.0 2.0 35.0 2.0 35.0 118-119 23.948489875791097 32.0 2.0 35.0 2.0 35.0 120-121 23.81248221274 32.0 2.0 35.0 2.0 35.0 122-123 23.55258067847854 32.0 2.0 35.0 2.0 35.0 124-125 22.560499509970093 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.8509913970239467 1101 2 1.4695789268087438 1101 3 1.7793642701924597 1101 4 2.1489131640010264 1101 5 1.6505818703378274 1101 6 1.6111871483462075 1101 7 1.4651986605785652 1101 8 1.83664660762782 1101 9 2.410787902850359 1101 10-11 2.2354948548007414 1101 12-13 2.102252127375344 1101 14-15 2.328568503191505 1101 16-17 2.0022666474942206 1101 18-19 2.017345545688471 1101 20-21 2.420904915163412 1101 22-23 2.310010531058662 1101 24-25 2.173184225261913 1101 26-27 1.9625232906169323 1101 28-29 1.932704798577678 1101 30-31 2.007485563943355 1101 32-33 2.2459445088807826 1101 34-35 2.4624423062970813 1101 36-37 2.5170201381078066 1101 38-39 2.5755491549306626 1101 40-41 2.307119648066717 1101 42-43 2.284517600656301 1101 44-45 2.5120936444867255 1101 46-47 2.446452040238107 1101 48-49 2.4040170120604927 1101 50-51 2.3284538467906124 1101 52-53 2.189108928362913 1101 54-55 2.2411735908142987 1101 56-57 2.5419075663847934 1101 58-59 2.391699156796605 1101 60-61 2.5698748977930954 1101 62-63 2.242622102854842 1101 64-65 2.15758021149119 1101 66-67 2.2647538761803006 1101 68-69 2.147319517996923 1101 70-71 2.210888396821023 1101 72-73 2.1080426710452222 1101 74-75 2.0902150993142854 1101 76-77 2.2795035573738325 1101 78-79 2.207461666238448 1101 80-81 2.0642252151857896 1101 82-83 2.111313280499008 1101 84-85 1.9468505196514343 1101 86-87 1.9227735605093237 1101 88-89 2.118204001242546 1101 90-91 2.0768237226263047 1101 92-93 2.1045755029357487 1101 94-95 2.0341771812876104 1101 96-97 2.053866360858688 1101 98-99 1.9409938800295343 1101 100-101 2.029294541949117 1101 102-103 2.0586388821672266 1101 104-105 1.9262644303856433 1101 106-107 2.1202048006317433 1101 108-109 2.137686354072045 1101 110-111 2.28040771750479 1101 112-113 2.199076252502117 1101 114-115 2.1432588346134764 1101 116-117 2.2678842203089395 1101 118-119 2.1757626446739273 1101 120-121 2.196671864663376 1101 122-123 2.170901041233556 1101 124-125 2.148374965538686 1102 1 0.7505778032787624 1102 2 0.5864045620104008 1102 3 0.6979829431890003 1102 4 1.1451821928233379 1102 5 1.0025956467360864 1102 6 0.9713125517542878 1102 7 0.8060703697560285 1102 8 1.032445094835218 1102 9 1.4181556975178644 1102 10-11 1.214327535004994 1102 12-13 1.4378396745558 1102 14-15 1.7068180746063035 1102 16-17 1.2263222346510325 1102 18-19 1.4067399726702092 1102 20-21 1.4363986886224325 1102 22-23 1.3132733061126025 1102 24-25 1.0797947215153556 1102 26-27 1.1035261832800423 1102 28-29 0.8496972916937402 1102 30-31 0.7894546087084535 1102 32-33 0.747062717699702 1102 34-35 1.1995094647624036 1102 36-37 1.3999689749081945 1102 38-39 1.3667872363910476 1102 40-41 0.9221915775563261 1102 42-43 1.0394941424641075 1102 44-45 1.3092584377128098 1102 46-47 1.1656651722331617 1102 48-49 1.2355742509023528 1102 50-51 1.1078542506743503 1102 52-53 1.191169680192889 1102 54-55 1.0215949394422026 1102 56-57 1.183309028527912 1102 58-59 1.2327402990081637 1102 60-61 1.6807056707685746 1102 62-63 1.5102077100024545 1102 64-65 1.6343355738088903 1102 66-67 1.5288159158981571 1102 68-69 1.4244734990007402 1102 70-71 1.6078211996468958 1102 72-73 1.3631866776427763 1102 74-75 1.4924425614403454 1102 76-77 1.3506669078768176 1102 78-79 1.2127519789990373 1102 80-81 0.7696704779824906 1102 82-83 0.7559958879766349 1102 84-85 0.754762747106998 1102 86-87 0.9464898387429699 1102 88-89 0.7191174470413628 1102 90-91 0.7092171044703015 1102 92-93 0.7480306640332657 1102 94-95 0.8752852438069212 1102 96-97 0.9901028710040016 1102 98-99 1.0402690084410757 1102 100-101 0.9548208504871667 1102 102-103 0.8736291623205652 1102 104-105 0.805274236178974 1102 106-107 1.078703630510482 1102 108-109 1.0724900643438495 1102 110-111 0.9711302665649235 1102 112-113 0.9204905377571464 1102 114-115 0.9198856541733633 1102 116-117 1.1441707140194133 1102 118-119 1.2529412779381737 1102 120-121 1.2825431670703438 1102 122-123 1.4160225015921277 1102 124-125 1.326552007149342 1103 1 1.6136629813289005 1103 2 1.3551102661544654 1103 3 1.443249023643471 1103 4 1.965581825652798 1103 5 1.516525573104996 1103 6 1.5542007105043325 1103 7 1.501176502528363 1103 8 1.7898554788278425 1103 9 2.4806790888814803 1103 10-11 1.9510641393866166 1103 12-13 2.0453564769416133 1103 14-15 2.27555839796851 1103 16-17 2.113916144820319 1103 18-19 1.8855983657869473 1103 20-21 2.2414119749169643 1103 22-23 2.04368882537133 1103 24-25 2.204159953774358 1103 26-27 1.948322269683306 1103 28-29 1.7802008117246224 1103 30-31 2.079398932338556 1103 32-33 2.10883970296166 1103 34-35 1.922049235005037 1103 36-37 2.2270774970467677 1103 38-39 2.2265813726932677 1103 40-41 2.283400522019818 1103 42-43 2.288618074340576 1103 44-45 2.241999157004628 1103 46-47 2.3245139527305767 1103 48-49 2.3461358701559547 1103 50-51 2.1664277025107985 1103 52-53 2.289862767774096 1103 54-55 2.155444015409472 1103 56-57 2.3770102021341764 1103 58-59 2.479753591212095 1103 60-61 2.5046992019485224 1103 62-63 2.25497838599809 1103 64-65 2.1902685100307373 1103 66-67 2.2307860596540934 1103 68-69 2.0662236851467526 1103 70-71 2.240415267400106 1103 72-73 2.251119885696891 1103 74-75 2.2891361552599 1103 76-77 2.363205417448075 1103 78-79 2.4044880318369266 1103 80-81 1.9526845068912628 1103 82-83 2.0411233142766037 1103 84-85 2.0357506407220924 1103 86-87 2.0480994600294053 1103 88-89 2.1549248972152952 1103 90-91 2.1575145579141015 1103 92-93 1.9875370520915574 1103 94-95 1.7988469457920857 1103 96-97 2.0639055779018456 1103 98-99 2.028667630763625 1103 100-101 1.7639167915192608 1103 102-103 1.828601279815139 1103 104-105 2.008654509823458 1103 106-107 1.7955287675100031 1103 108-109 2.015504270534535 1103 110-111 2.186496521149632 1103 112-113 2.0331221695780677 1103 114-115 1.9833451925312602 1103 116-117 2.138612926024944 1103 118-119 2.196492254367559 1103 120-121 2.183847617240332 1103 122-123 2.2183777903055777 1103 124-125 2.056827670676146 1104 1 1.1967844610014637 1104 2 1.0715156878762357 1104 3 1.1346649916569689 1104 4 1.4443123366623851 1104 5 1.084530752849684 1104 6 0.8834645978983922 1104 7 0.979310266620498 1104 8 1.263330781948678 1104 9 1.7486325268488727 1104 10-11 1.603265298922011 1104 12-13 1.1059122170315057 1104 14-15 1.4088213866082029 1104 16-17 1.1113248807374596 1104 18-19 0.501816412997222 1104 20-21 -3.9304023491121285 1104 22-23 -0.7123991838970376 1104 24-25 0.7446190545746418 1104 26-27 0.7931105459182071 1104 28-29 0.46242325868698586 1104 30-31 1.1929411286393758 1104 32-33 1.0934361634900824 1104 34-35 1.2761668815180833 1104 36-37 1.3824655851185135 1104 38-39 1.3186440046765462 1104 40-41 1.4029764497532824 1104 42-43 1.4108590283439 1104 44-45 1.5777915391799695 1104 46-47 1.512220394071548 1104 48-49 1.572106484661468 1104 50-51 1.4311366867142432 1104 52-53 1.463056769439234 1104 54-55 1.3841543461980876 1104 56-57 1.69067470139872 1104 58-59 1.7092252807589574 1104 60-61 1.734126510473022 1104 62-63 1.4284634931496498 1104 64-65 1.559433101459355 1104 66-67 1.4328490658606299 1104 68-69 1.3401920660651996 1104 70-71 1.6268553650816493 1104 72-73 1.4906914279049452 1104 74-75 1.580845038021014 1104 76-77 1.4931562210074638 1104 78-79 1.523159560931699 1104 80-81 1.2495074133131787 1104 82-83 1.2031143551863188 1104 84-85 1.132950337384763 1104 86-87 1.1463437344136054 1104 88-89 1.300262307417178 1104 90-91 1.2996733900159008 1104 92-93 1.049823574734848 1104 94-95 1.1976703538587294 1104 96-97 1.2752081871966858 1104 98-99 1.23185160571974 1104 100-101 0.743806930705567 1104 102-103 1.1405895836374143 1104 104-105 1.3320526796552485 1104 106-107 1.5284065072262862 1104 108-109 1.5352589285315936 1104 110-111 1.5562814417462612 1104 112-113 1.4753908099167354 1104 114-115 1.4168545345045125 1104 116-117 1.580197913053933 1104 118-119 1.5222294741794933 1104 120-121 1.6014134248636012 1104 122-123 1.4102858155078088 1104 124-125 1.6241883977702791 1105 1 1.1487164058691697 1105 2 0.9857210491177568 1105 3 0.9911186179593798 1105 4 1.5103776606398398 1105 5 1.1285142656240055 1105 6 1.1536529241402356 1105 7 1.015772919621103 1105 8 1.247171333425932 1105 9 1.623373973666574 1105 10-11 1.4244357415002504 1105 12-13 1.5754706996203964 1105 14-15 1.8012090061444468 1105 16-17 1.5295449045772038 1105 18-19 1.1854329364839984 1105 20-21 1.3709130799839286 1105 22-23 1.191812098704201 1105 24-25 1.2195868053451946 1105 26-27 1.18783845268862 1105 28-29 0.7229738294134798 1105 30-31 1.2135848209446891 1105 32-33 1.1435105758867508 1105 34-35 1.0913267196547274 1105 36-37 1.3765958396019649 1105 38-39 1.1140816645243277 1105 40-41 1.157306622456396 1105 42-43 1.3868400838503803 1105 44-45 1.5703601170121004 1105 46-47 1.818346686837458 1105 48-49 1.448611628722169 1105 50-51 1.4855968400138089 1105 52-53 1.470437888981266 1105 54-55 1.60370767893993 1105 56-57 1.884088934264387 1105 58-59 1.6111644971335402 1105 60-61 1.9099659355417806 1105 62-63 1.7493952297089344 1105 64-65 1.5480612554995723 1105 66-67 1.8210391550089184 1105 68-69 1.2591759260202195 1105 70-71 1.3459047264620416 1105 72-73 1.2949490205539718 1105 74-75 1.6272912770896433 1105 76-77 1.4966432846688313 1105 78-79 1.4255557249707245 1105 80-81 1.3572988618667878 1105 82-83 1.3545445082158558 1105 84-85 1.2741987220301176 1105 86-87 1.4214162951798741 1105 88-89 1.4958304879202835 1105 90-91 1.547250878819323 1105 92-93 1.5222986066561859 1105 94-95 1.4128710821666886 1105 96-97 1.4678875349598748 1105 98-99 1.2718628890924855 1105 100-101 1.3924280639805247 1105 102-103 1.424026711141451 1105 104-105 1.4924189360057625 1105 106-107 1.5294223386662011 1105 108-109 1.6145144573842387 1105 110-111 1.5544207267854695 1105 112-113 1.6028565643982624 1105 114-115 1.4890528634080766 1105 116-117 1.6629536212832754 1105 118-119 1.660697462190189 1105 120-121 1.5653957032176606 1105 122-123 1.7105583909328175 1105 124-125 1.5083340053557954 1106 1 -0.37190576729420854 1106 2 -0.2515551671926133 1106 3 -0.21859851734322078 1106 4 -0.6430642153913233 1106 5 -0.9122546926518389 1106 6 -0.5673834178964761 1106 7 -0.5034882228367827 1106 8 -0.14519235702579536 1106 9 -0.12558551559952136 1106 10-11 -2.3331455334165234 1106 12-13 -0.6434305061703753 1106 14-15 -0.3235008192595501 1106 16-17 -0.5465829824413184 1106 18-19 -3.3537163557181913 1106 20-21 -5.683851564766744 1106 22-23 -6.46204975889076 1106 24-25 -6.385949811668965 1106 26-27 -5.165609157747607 1106 28-29 -1.9402851915102488 1106 30-31 -3.8955857453131273 1106 32-33 -4.371911920595302 1106 34-35 -3.6332925686919033 1106 36-37 -5.9807035491848275 1106 38-39 -6.524365270700379 1106 40-41 -2.831828715374435 1106 42-43 -1.5188692111700846 1106 44-45 -0.7609888186001257 1106 46-47 -0.5040253214373074 1106 48-49 -0.332710415857445 1106 50-51 -0.47091114216060603 1106 52-53 -0.6499657113639756 1106 54-55 -0.5744588771486221 1106 56-57 -0.494413877159797 1106 58-59 -0.3036792550070757 1106 60-61 -0.08619833461328241 1106 62-63 -0.346052141956843 1106 64-65 -0.47724594616947513 1106 66-67 -0.9034743110572983 1106 68-69 -0.7066115565368136 1106 70-71 -0.3906800145405178 1106 72-73 -0.4597730565140843 1106 74-75 -0.43533954269542363 1106 76-77 -0.5846476414669439 1106 78-79 -0.605797594623521 1106 80-81 -0.9226457120747433 1106 82-83 -0.9892045792700266 1106 84-85 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-5.202892460174592 2216 92-93 -4.932010685584142 2216 94-95 -8.044036229338325 2216 96-97 -10.183916318912942 2216 98-99 -7.01990522359517 2216 100-101 -5.986329064374303 2216 102-103 -6.930755590422457 2216 104-105 -9.500065216708276 2216 106-107 -6.307648568289764 2216 108-109 -8.711491044138505 2216 110-111 -6.209190536366023 2216 112-113 -5.6353909479871795 2216 114-115 -5.92363914020347 2216 116-117 -5.453099761264362 2216 118-119 -5.7180894192978435 2216 120-121 -6.505152835089504 2216 122-123 -9.295180852794932 2216 124-125 -7.875114410851243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 263001.0 3 41388.0 4 24085.0 5 20103.0 6 20143.0 7 22350.0 8 24101.0 9 25909.0 10 27596.0 11 28949.0 12 29606.0 13 30473.0 14 31865.0 15 27083.0 16 22361.0 17 19846.0 18 18141.0 19 16993.0 20 17230.0 21 18620.0 22 22967.0 23 31166.0 24 42423.0 25 53837.0 26 64454.0 27 73007.0 28 83340.0 29 95377.0 30 107095.0 31 120757.0 32 144029.0 33 175132.0 34 216904.0 35 273623.0 36 355275.0 37 381375.0 38 117557.0 39 426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70010500797283 19.1644735989214 13.645267511051765 23.490153882054006 2 14.80731996914062 20.924557108960684 40.37921280081255 23.88891012108614 3 18.09931080322504 26.477860156898593 29.521054075563853 25.90177496431252 4 10.923783114888986 16.764831647759298 38.67462698171028 33.63675825564144 5 13.131054295338496 39.156773651395696 33.74722776363539 13.964944289630427 6 30.852562531386706 37.34764799227065 17.90101891248439 13.898770563858248 7 27.97922898048071 33.46769049652683 21.20378147043225 17.34929905256021 8 25.47675608679731 36.13882207355022 20.23827524365159 18.14614659600088 9 26.253828868209222 16.718117589156776 20.633050337069918 36.39500320556408 10-11 24.00054224787984 27.73178424494574 27.693107783265404 20.574565723909018 12-13 24.815224171574677 25.207578864750392 28.116394727439364 21.86080223623557 14-15 22.093576367357816 26.40151970201769 25.939897099866364 25.56500683075813 16-17 22.031260119949742 29.989021878521744 25.99007111491082 21.989646886617702 18-19 21.767252755437607 28.452048827957388 28.22652959777978 21.55416881882522 20-21 22.858969994650586 27.539350613561574 28.094473893238664 21.507205498549173 22-23 22.372851717613955 27.74036597203276 27.8135020898925 22.073280220460788 24-25 22.133070562973405 27.714004729736953 28.099724719335807 22.05319998795384 26-27 22.133551569666444 28.094609012124423 27.68076946116025 22.091069957048887 28-29 21.867158820934364 27.989897886084098 27.984102122010547 22.15884117097099 30-31 21.949108242401614 27.69451735296551 28.196972319081326 22.159402085551548 32-33 21.89877946707119 27.801114167345283 27.905116071240656 22.394990294342872 34-35 22.31206177648188 27.71907451856131 27.826035706837615 22.142827998119195 36-37 22.430335475030727 27.500666986993107 27.675152079510028 22.39384545846614 38-39 22.130797332860585 27.673862308968207 27.84016591504625 22.35517444312496 40-41 22.303863183043106 27.457122031707453 28.043213912038606 22.195800873210832 42-43 22.157622414782143 27.639692068914208 27.631709965664005 22.570975550639645 44-45 22.103730723815715 27.77293929539524 27.621912739419795 22.50141724136925 46-47 22.18420994691233 27.94875207060052 27.24696581744524 22.62007216504192 48-49 22.410411312781214 27.89912037210182 27.145824337672458 22.544643977444508 50-51 22.327560846520583 27.895519181385264 27.136414040158453 22.6405059319357 52-53 22.641974381176336 27.890569192778443 27.011804294931867 22.455652131113354 54-55 22.29038884603852 27.784329458667095 27.15711664733543 22.768165047958952 56-57 22.203505243164585 28.11334146267228 27.124286281384354 22.558867012778776 58-59 22.45560337667241 27.642627623392674 27.16199679482301 22.739772205111898 60-61 22.426521432011455 27.615890136063566 27.495618766803094 22.461969665121888 62-63 22.27185825016785 27.769126145972443 27.351494906456335 22.607520697403373 64-65 22.23350288323334 27.672839388247468 27.270443045577718 22.82321468294147 66-67 22.128282304805378 28.173524032391057 26.951727661773557 22.74646600103001 68-69 22.122652628525024 27.81459537590971 27.394419281764883 22.66833271380039 70-71 21.96853563979804 27.727261174070016 27.630951615819892 22.673251570312047 72-73 22.228418921167844 27.760200378040008 27.223397166707354 22.787983534084795 74-75 22.13617682469495 27.966403657638107 27.11487152306559 22.78254799460135 76-77 22.270469887213146 27.785085713314324 27.263528472751204 22.68091592672133 78-79 22.216805105396027 27.814890695230183 27.311870648101806 22.656433551271984 80-81 22.14121730143366 28.187365013159322 27.146939343120298 22.52447834228672 82-83 22.288001523098053 28.054436508763963 27.125408215533913 22.532153752604064 84-85 22.302741195905078 27.86667728668813 26.932083020075076 22.898498497331722 86-87 22.26826965480129 27.702573626784936 27.146604707425848 22.882552010987922 88-89 22.29805240987545 27.749925406516272 27.13943258440882 22.812589599199455 90-91 22.35840346426257 27.920935731667296 27.194665154796933 22.525995649273202 92-93 22.391304031084232 27.623517024301186 27.120070623016947 22.86510832159764 94-95 22.293150676060716 27.594706781329325 27.055558073874526 23.05658446873543 96-97 22.418683261346786 27.48076761188134 27.16000116559928 22.94054796117259 98-99 22.12999479844669 27.491431521542577 27.106115087240557 23.27245859277018 100-101 22.01119022360211 27.63733651243913 27.19913019250934 23.152343071449422 102-103 22.28648616533668 27.255995463264572 27.35239947231149 23.105118899087262 104-105 22.192543713022722 27.364995746272218 27.35248144913294 23.089979091572115 106-107 22.104573004895485 27.480427772165672 27.39045056595954 23.024548656979306 108-109 22.191841097761227 27.43446297693037 27.02933634834965 23.344359576958762 110-111 21.899345816285578 27.70203906063952 27.321369510269612 23.077245612805285 112-113 21.996893917722332 27.634693899687434 27.320637347490567 23.04777483509967 114-115 21.899205667981324 27.726697721585218 27.107110328712224 23.266986281721234 116-117 21.704862234818357 27.640006210284174 27.410908891925672 23.244222662971794 118-119 21.909872613424174 27.74523202890231 26.985811434971264 23.359083922702254 120-121 21.857295017433906 27.801126060163934 27.177870199523795 23.163708722878365 122-123 21.954956089666897 27.764078002805938 27.144334072754223 23.136631834772942 124-125 22.046716078994287 27.805975059295278 26.88313432180665 23.264174539903784 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1508.0 1 1698.0 2 2240.5 3 2399.0 4 1885.0 5 1448.5 6 1291.0 7 1412.5 8 1706.5 9 1798.0 10 1682.5 11 1579.0 12 1675.5 13 1877.0 14 2025.0 15 2193.0 16 2504.5 17 2829.5 18 3172.0 19 3668.5 20 4431.5 21 5561.0 22 6935.0 23 8678.5 24 10899.0 25 14071.5 26 18362.5 27 23247.5 28 27860.5 29 32901.5 30 40051.5 31 47574.5 32 54797.5 33 61837.0 34 68253.5 35 73172.0 36 78119.0 37 83771.0 38 88183.0 39 90521.5 40 92854.0 41 97838.5 42 101941.0 43 106464.0 44 111245.5 45 112670.0 46 110918.5 47 109554.5 48 111075.5 49 110243.0 50 106550.0 51 102453.0 52 99231.0 53 97918.0 54 95388.5 55 89552.5 56 82040.5 57 74286.5 58 67905.5 59 61815.0 60 53529.5 61 44859.5 62 37300.0 63 29639.5 64 23419.5 65 19049.0 66 14683.0 67 11457.0 68 8454.5 69 5679.0 70 4257.5 71 3345.0 72 2705.0 73 2362.0 74 1904.0 75 1466.0 76 1069.0 77 695.5 78 473.0 79 329.0 80 239.5 81 181.5 82 129.5 83 77.5 84 62.0 85 48.0 86 33.5 87 28.5 88 16.0 89 12.5 90 14.0 91 11.0 92 11.0 93 8.5 94 6.5 95 6.5 96 4.5 97 2.5 98 1.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10059616258178902 2 0.03344571482040169 3 0.044356853150000305 4 0.05203026497229963 5 0.055980291311204765 6 0.0031729719771533067 7 0.0017159950488686252 8 0.0018778813742335897 9 0.006702093870109536 10-11 0.04655850717496383 12-13 0.005908850875821208 14-15 0.011332042775547524 16-17 0.020300545200766563 18-19 0.01949111357394174 20-21 0.13394474560697173 22-23 0.016496216554689896 24-25 0.015087805524014702 26-27 0.006216434894014642 28-29 0.004030969501587619 30-31 0.0016674291512591356 32-33 0.005892662243284713 34-35 0.017839873055219103 36-37 0.0022987858201824977 38-39 0.008401900286441664 40-41 0.03291148994669731 42-43 0.01387365808377747 44-45 0.10904662876584016 46-47 0.003593876423102215 48-49 0.00947035003385043 50-51 0.011186345082719056 52-53 0.01259475611339425 54-55 0.01039310208843073 56-57 0.005989794038503691 58-59 0.014828787403430759 60-61 0.031923983361971026 62-63 0.007009677888302969 64-65 0.006928734725620486 66-67 0.008596163876879621 68-69 0.007738166352445309 70-71 0.0035129332604197324 72-73 8.094316268248232E-4 74-75 0.0011817701751642418 76-77 0.007754354984981806 78-79 0.020672883749105985 80-81 0.0029625197541788524 82-83 0.004694703435583974 84-85 0.0026225584709124268 86-87 0.003399612832664257 88-89 0.005844096345675223 90-91 0.004451873947536527 92-93 0.0024444835130109656 94-95 0.003464367362810243 96-97 0.0015217314584306675 98-99 0.002800633428813888 100-101 0.002833010693886881 102-103 7.284884641423408E-4 104-105 0.0038690831762226547 106-107 8.741861569708089E-4 108-109 0.001505542825894171 110-111 0.0026387471034489234 112-113 0.0042252330920255765 114-115 0.002590181205839434 116-117 0.005228928309288358 118-119 0.0036910082183211936 120-121 0.0067506597677190255 122-123 0.004338553519781052 124-125 0.05521942558198943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3088587.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.05728705732139 #Duplication Level Percentage of deduplicated Percentage of total 1 77.92650656867932 42.90422041526018 2 11.024943873007329 12.140069992140424 3 4.231249743343691 6.98883395191473 4 2.175361134601784 4.790779297644431 5 1.243063250392124 3.421984510362307 6 0.8099613375363678 2.6756564319643066 7 0.5179039784834817 1.9960071608045697 8 0.3440008130554556 1.5151801209876934 9 0.2660898233159589 1.3185165406804809 >10 1.3177345121184938 12.796102195305533 >50 0.08981441823891811 3.4133599033460054 >100 0.0513250848201345 4.989113194664758 >500 0.001549592732649365 0.5624737766644671 >1k 4.958696743576586E-4 0.4877025082600288 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3545 0.11477740468375992 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.475453014598585E-5 0.0 0.0 0.0 0.0 3 6.475453014598585E-5 0.0 0.0 0.0 0.0 4 9.713179521897878E-5 0.0 0.0 0.0 0.0 5 1.9426359043795755E-4 0.0 0.0 0.0 0.0 6 2.913953856569363E-4 0.0 0.0 0.0 0.0 7 2.913953856569363E-4 0.0 0.0 0.0 0.0 8 2.913953856569363E-4 0.0 0.0 0.0 0.0 9 2.913953856569363E-4 0.0 0.0 0.0 0.0 10-11 2.913953856569363E-4 0.0 0.0 4.856589760948939E-5 0.0 12-13 3.399612832664257E-4 0.0 0.0 6.475453014598585E-5 0.0 14-15 4.370930784854045E-4 0.0 0.0 6.475453014598585E-5 0.0 16-17 4.8565897609489385E-4 0.0 0.0 6.475453014598585E-5 0.0 18-19 7.122998316058443E-4 0.0 0.0 6.475453014598585E-5 0.0 20-21 8.74186156970809E-4 0.0 0.0 1.295090602919717E-4 0.0 22-23 8.74186156970809E-4 0.0 0.0 5.018476086313903E-4 0.0 24-25 0.0011979588077007382 0.0 0.0 0.0011493929100912488 0.0 26-27 0.0012303360727737311 0.0 0.0 0.0013922223981386959 0.0 28-29 0.0013760337656021993 0.0 0.0 0.0022664085551095045 0.0 30-31 0.0014245996632116886 0.0 0.0 0.005293682839434343 1.6188632536496463E-5 32-33 0.0014245996632116886 0.0 0.0 0.011979588077007382 3.2377265072992925E-5 34-35 0.0016350518861861427 0.0 0.0 0.020073904345255612 3.2377265072992925E-5 36-37 0.0018616927416970934 3.2377265072992925E-5 0.0 0.03177828566914256 3.2377265072992925E-5 38-39 0.0019102586393065827 3.2377265072992925E-5 0.0 0.05627168669686171 3.2377265072992925E-5 40-41 0.0019102586393065827 3.2377265072992925E-5 0.0 0.08584831834104073 3.2377265072992925E-5 42-43 0.0020235790670620575 3.2377265072992925E-5 0.0 0.11374133220142414 3.2377265072992925E-5 44-45 0.0022340312900365118 3.2377265072992925E-5 0.0 0.146539501720366 3.2377265072992925E-5 46-47 0.002525426675693448 3.2377265072992925E-5 0.0 0.18914798255642468 3.2377265072992925E-5 48-49 0.0026225584709124268 3.2377265072992925E-5 0.0 0.23604645101465493 3.2377265072992925E-5 50-51 0.0026225584709124268 3.2377265072992925E-5 0.0 0.29244764677180857 3.2377265072992925E-5 52-53 0.0026225584709124268 3.2377265072992925E-5 0.0 0.3469385838896557 3.2377265072992925E-5 54-55 0.00265493573598542 3.2377265072992925E-5 0.0 0.40558999956938235 3.2377265072992925E-5 56-57 0.0027520675312043986 3.2377265072992925E-5 0.0 0.4731937290417916 3.2377265072992925E-5 58-59 0.0027520675312043986 3.2377265072992925E-5 0.0 0.5549625119836352 3.2377265072992925E-5 60-61 0.0027520675312043986 3.2377265072992925E-5 0.0 0.6469948879536176 3.2377265072992925E-5 62-63 0.0027844447962773917 3.2377265072992925E-5 0.0 0.7442076263352789 3.2377265072992925E-5 64-65 0.0027844447962773917 3.2377265072992925E-5 0.0 0.8428611530126884 6.475453014598585E-5 66-67 0.0028491993264233772 3.2377265072992925E-5 0.0 0.9560196944427986 6.475453014598585E-5 68-69 0.0028815765914963704 3.2377265072992925E-5 0.0 1.0911623988574712 6.475453014598585E-5 70-71 0.0029948970192518456 3.2377265072992925E-5 0.0 1.2483054548892423 6.475453014598585E-5 72-73 0.0030434629168613354 3.2377265072992925E-5 0.0 1.409608989482893 6.475453014598585E-5 74-75 0.003075840181934328 3.2377265072992925E-5 0.0 1.5690022654372373 6.475453014598585E-5 76-77 0.003108217447007321 3.2377265072992925E-5 0.0 1.7428034243490633 6.475453014598585E-5 78-79 0.0031405947120803136 3.2377265072992925E-5 0.0 1.9550525855350682 6.475453014598585E-5 80-81 0.0036910082183211936 3.2377265072992925E-5 0.0 2.194773856135508 6.475453014598585E-5 82-83 0.003788140013540172 3.2377265072992925E-5 0.0 2.4519788498753634 6.475453014598585E-5 84-85 0.003950026338905137 3.2377265072992925E-5 0.0 2.7057194762524093 6.475453014598585E-5 86-87 0.0041442899293430945 3.2377265072992925E-5 0.0 2.970419806856663 6.475453014598585E-5 88-89 0.004176667194416088 6.475453014598585E-5 0.0 3.2733576875121213 6.475453014598585E-5 90-91 0.0046299489054379885 6.475453014598585E-5 0.0 3.6154882475384373 6.475453014598585E-5 92-93 0.00475945796572996 6.475453014598585E-5 0.0 3.9862726871543526 6.475453014598585E-5 94-95 0.00475945796572996 6.475453014598585E-5 0.0 4.363338316194428 6.475453014598585E-5 96-97 0.00475945796572996 6.475453014598585E-5 0.0 4.738461309330124 6.475453014598585E-5 98-99 0.005018476086313903 6.475453014598585E-5 0.0 5.141655391284106 6.475453014598585E-5 100-101 0.005261305574361351 6.475453014598585E-5 0.0 5.603177116267083 6.475453014598585E-5 102-103 0.005439380532262812 6.475453014598585E-5 0.0 6.097351313076174 6.475453014598585E-5 104-105 0.006281189424160628 6.475453014598585E-5 0.0 6.593597654849937 6.475453014598585E-5 106-107 0.006443075749525592 6.475453014598585E-5 0.0 7.082510546084666 6.475453014598585E-5 108-109 0.006475453014598585 6.475453014598585E-5 0.0 7.600951503065965 6.475453014598585E-5 110-111 0.006540207544744572 6.475453014598585E-5 0.0 8.177736291708797 6.475453014598585E-5 112-113 0.006637339339963549 6.475453014598585E-5 0.0 8.794944095795262 6.475453014598585E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8880 0.0 51.28272 1 GGTATCA 4930 0.0 50.351418 1 ATCAACG 10625 0.0 41.496143 3 TCAACGC 10810 0.0 40.549538 4 CAACGCA 11075 0.0 39.73996 5 GTACATG 15260 0.0 39.204334 1 TATCAAC 11670 0.0 39.105965 2 AACGCAG 11015 0.0 38.854275 6 TACATGG 15715 0.0 35.96895 2 ACATGGG 15625 0.0 33.12972 3 ACGCAGA 12815 0.0 32.9339 7 CGCAGAG 12685 0.0 32.66348 8 CATGGGA 10760 0.0 29.28041 4 GCAGAGT 13610 0.0 28.432365 9 CATGGGG 5980 0.0 24.699278 4 AGAGTAC 12710 0.0 23.737621 10-11 GAGTACT 5620 0.0 23.28492 12-13 ATGGGAG 5045 0.0 22.901676 5 CAGAGTA 13740 0.0 22.67278 10-11 ATGGGAT 4900 0.0 22.48549 5 >>END_MODULE