FastQCFastQC Report
Thu 26 May 2016
SRR1364514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088587
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109730.3552757296459514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98420.31865704284839635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68620.22217279293087747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72850.068.159551
GTATCAA130800.052.4714471
ATCAACG172900.038.2707823
TATCAAC180350.037.74762
TCAACGC174800.037.3773464
CAACGCA174900.037.1479035
AACGCAG177800.035.8392756
GTACATG142950.032.6061741
TACATGG149600.030.3642622
ACGCAGA208650.030.0554837
CGCAGAG210600.029.9326428
ACATGGG149300.027.63033
GTGGTAT33450.027.2978381
GCAGAGT222350.027.1740349
GAGTACT136650.026.20067812-13
AGTACTT143750.025.87885912-13
AGAGTAC209000.025.48267410-11
TGGTATC35350.024.6208362
CAGAGTA220400.024.21857510-11
GTACTTT153550.023.8594314-15
Produced by FastQC (version 0.11.5)