FastQCFastQC Report
Thu 26 May 2016
SRR1364513_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364513_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357316
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40660.12110864750294581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41100.053.4458921
GTATCAA75700.052.7662731
ATCAACG91600.042.1565783
TCAACGC93750.041.242034
CAACGCA95250.040.7164275
TATCAAC101900.039.2961962
AACGCAG97700.038.7007186
GTACATG133000.036.5679131
TACATGG133250.035.0519452
ACATGGG136900.032.987893
ACGCAGA114450.032.6721157
CGCAGAG113600.032.448728
GCAGAGT122500.027.3689129
CATGGGG65050.024.5444954
CATGGGA85200.024.4035174
AGAGTAC115550.023.28184110-11
GAGTACT61150.023.15138212-13
GAGTACA100600.022.9595571
CAGAGTA121750.022.90284710-11
AGTACAT101350.021.5452732
Produced by FastQC (version 0.11.5)