##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364513_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3357316 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.084267909246552 28.0 16.0 31.0 2.0 33.0 2 23.388054028873064 28.0 16.0 31.0 2.0 34.0 3 23.361401190713057 28.0 16.0 31.0 2.0 34.0 4 25.85252088275277 32.0 17.0 35.0 2.0 37.0 5 26.30945076364572 32.0 19.0 35.0 2.0 37.0 6 26.383424437854526 33.0 19.0 35.0 2.0 37.0 7 26.09833301363351 32.0 18.0 35.0 2.0 37.0 8 25.7388333418719 32.0 17.0 35.0 2.0 37.0 9 26.1760820250462 33.0 13.0 37.0 2.0 39.0 10-11 26.18494446158777 32.5 14.5 37.0 2.0 39.0 12-13 25.990439088843587 32.5 11.5 37.0 2.0 39.0 14-15 26.432628176793607 33.0 11.0 38.5 2.0 40.0 16-17 26.626137962586782 33.0 12.0 39.0 2.0 40.0 18-19 26.573023361518544 33.0 11.0 39.0 2.0 40.5 20-21 26.34597681600421 33.0 10.0 39.0 2.0 40.0 22-23 26.365102808314738 33.0 10.0 39.0 2.0 40.0 24-25 26.35365065427264 33.0 10.0 39.0 2.0 41.0 26-27 26.393979446677047 33.0 10.0 39.0 2.0 41.0 28-29 26.277562791229663 33.0 9.5 39.0 2.0 40.0 30-31 25.931234652919176 33.0 8.5 39.0 2.0 40.0 32-33 25.875515441501484 33.0 8.0 38.0 2.0 40.0 34-35 25.58756324992941 32.0 8.0 38.0 2.0 40.0 36-37 25.352278576100673 32.0 4.0 38.0 2.0 40.0 38-39 25.18931000239477 32.0 2.0 38.0 2.0 40.0 40-41 25.195561275733354 32.0 2.0 38.0 2.0 40.0 42-43 25.04640507476806 32.0 2.0 38.0 2.0 40.0 44-45 24.709747458982115 32.0 2.0 38.0 2.0 40.0 46-47 24.448121952178468 31.5 2.0 38.0 2.0 40.0 48-49 24.199411970752827 31.0 2.0 38.0 2.0 40.0 50-51 24.169851899553095 31.0 2.0 38.0 2.0 40.0 52-53 23.983347709896833 31.0 2.0 38.0 2.0 40.0 54-55 23.85012894228604 31.0 2.0 38.0 2.0 40.0 56-57 23.462228905470916 30.0 2.0 37.5 2.0 40.0 58-59 23.248190072069477 30.0 2.0 37.0 2.0 40.0 60-61 22.868503888225 29.5 2.0 37.0 2.0 40.0 62-63 22.80326606134186 30.0 2.0 36.0 2.0 40.0 64-65 22.644530482087475 29.5 2.0 36.0 2.0 40.0 66-67 22.41977371209621 29.0 2.0 36.0 2.0 39.0 68-69 22.26745471680354 29.0 2.0 35.5 2.0 39.0 70-71 22.16093674232631 29.0 2.0 35.0 2.0 39.0 72-73 22.134960486293217 29.5 2.0 35.0 2.0 39.0 74-75 21.886909513432755 29.0 2.0 35.0 2.0 38.5 76-77 21.6347393274866 29.0 2.0 35.0 2.0 37.0 78-79 21.35453558735609 29.0 2.0 35.0 2.0 37.0 80-81 21.358654502584805 29.0 2.0 35.0 2.0 36.5 82-83 21.18533882422745 29.0 2.0 34.0 2.0 36.0 84-85 21.01396338622876 29.0 2.0 34.0 2.0 36.0 86-87 20.823756834328375 29.0 2.0 34.0 2.0 35.0 88-89 20.616792104168926 29.0 2.0 34.0 2.0 35.0 90-91 20.458673833502715 29.0 2.0 34.0 2.0 35.0 92-93 20.35475659723422 29.0 2.0 34.0 2.0 35.0 94-95 20.289314142606774 29.0 2.0 34.0 2.0 35.0 96-97 20.1731785152187 29.0 2.0 34.0 2.0 35.0 98-99 20.08062348018477 28.5 2.0 34.0 2.0 35.0 100-101 20.015893052664687 28.0 2.0 34.0 2.0 35.0 102-103 19.104944098202253 25.5 2.0 32.5 2.0 34.5 104-105 19.72408897464522 27.5 2.0 34.0 2.0 35.0 106-107 19.79021739389441 28.0 2.0 34.0 2.0 35.0 108-109 19.739464202952597 28.0 2.0 34.0 2.0 35.0 110-111 19.63821725449734 27.0 2.0 34.0 2.0 35.0 112-113 19.611005338788484 27.5 2.0 34.0 2.0 35.0 114-115 19.56227444780295 28.0 2.0 34.0 2.0 35.0 116-117 19.37908108739243 27.0 2.0 34.0 2.0 35.0 118-119 19.228334479089845 27.0 2.0 34.0 2.0 35.0 120-121 19.076933330076763 27.0 2.0 34.0 2.0 35.0 122-123 18.813318138655998 27.0 2.0 34.0 2.0 35.0 124-125 17.9656505375127 24.0 2.0 33.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6669296877052098 1101 2 1.2059202905719317 1101 3 1.581446384092846 1101 4 1.7938327046294695 1101 5 1.5025463204012688 1101 6 1.4235237052957288 1101 7 1.3372692492946214 1101 8 1.6732547430562015 1101 9 2.0914194389635377 1101 10-11 2.2122556542083345 1101 12-13 1.9520924532847488 1101 14-15 2.3960668492282267 1101 16-17 1.96248449755101 1101 18-19 1.936649504394616 1101 20-21 2.3622046021640415 1101 22-23 2.2661243286800428 1101 24-25 2.158563141858373 1101 26-27 1.748999236575699 1101 28-29 1.739634779981337 1101 30-31 1.8368165461064372 1101 32-33 2.0476768215409784 1101 34-35 2.319205935867508 1101 36-37 2.27031991408629 1101 38-39 2.4061744975915182 1101 40-41 2.212736197070985 1101 42-43 2.1550160523260367 1101 44-45 2.356982893184213 1101 46-47 2.32850858627409 1101 48-49 2.2729726309525446 1101 50-51 2.2277190147758716 1101 52-53 2.1973443918126705 1101 54-55 2.0831326982769625 1101 56-57 2.3299865648398317 1101 58-59 2.384971519672888 1101 60-61 2.431061806547458 1101 62-63 2.1634313594704544 1101 64-65 2.041191524451218 1101 66-67 2.163958748757885 1101 68-69 2.059445251396564 1101 70-71 2.011892209164923 1101 72-73 1.8189925078407967 1101 74-75 1.8771581946240232 1101 76-77 2.163924907257357 1101 78-79 2.1029524719741985 1101 80-81 2.0684941994554897 1101 82-83 2.0642597841114103 1101 84-85 1.8642266935738405 1101 86-87 1.8919927822329434 1101 88-89 2.0722221852625964 1101 90-91 1.9876929215620827 1101 92-93 1.9382869353286871 1101 94-95 1.9454783317699231 1101 96-97 1.8153946318067646 1101 98-99 1.8031051280814516 1101 100-101 1.791877108255079 1101 102-103 1.833541222175132 1101 104-105 1.8031078243584275 1101 106-107 1.8982318204277995 1101 108-109 1.9986050722688624 1101 110-111 2.097630672442712 1101 112-113 2.116749867576157 1101 114-115 2.0688698266888785 1101 116-117 2.222198250477714 1101 118-119 2.15414912843951 1101 120-121 2.138420452718478 1101 122-123 2.051241070161101 1101 124-125 2.103190906834179 1102 1 0.6145188301542284 1102 2 0.43025272783838275 1102 3 0.47326119753435236 1102 4 0.9729894727200623 1102 5 0.9273168248532819 1102 6 0.8110429652072888 1102 7 0.7423042139013454 1102 8 0.9503094973842501 1102 9 1.3012904612687954 1102 10-11 1.0148434977580507 1102 12-13 1.3003553056945023 1102 14-15 1.4561524148965432 1102 16-17 1.0255860039642712 1102 18-19 1.1262751730794385 1102 20-21 1.1571098773606074 1102 22-23 1.176996629272491 1102 24-25 0.8528254908396242 1102 26-27 0.930262342492064 1102 28-29 0.7292849404888422 1102 30-31 0.6329524989856488 1102 32-33 0.515201911843139 1102 34-35 0.9027287083522921 1102 36-37 1.2011174270554008 1102 38-39 1.2852126003927253 1102 40-41 0.8773920063061631 1102 42-43 0.8102714489380389 1102 44-45 1.1665970967535664 1102 46-47 1.1583913320930677 1102 48-49 1.1825812259355786 1102 50-51 1.1465078797679027 1102 52-53 1.2474126190330068 1102 54-55 0.9263530880498152 1102 56-57 1.1681176292871562 1102 58-59 1.2077778688166454 1102 60-61 1.5458887056709862 1102 62-63 1.4504016985998653 1102 64-65 1.5292172486692621 1102 66-67 1.5492716162029048 1102 68-69 1.3573725165456914 1102 70-71 1.6231408712911737 1102 72-73 1.4603007841612836 1102 74-75 1.545774965260513 1102 76-77 1.356819855261616 1102 78-79 1.299247224021471 1102 80-81 0.956085340383467 1102 82-83 0.9043246532747062 1102 84-85 0.975293207337451 1102 86-87 1.0636443507121989 1102 88-89 0.7795557890179126 1102 90-91 0.787089210642069 1102 92-93 0.7501248201493134 1102 94-95 0.992806940074658 1102 96-97 1.0848201030857787 1102 98-99 1.1832710907062385 1102 100-101 1.1224550275195924 1102 102-103 0.8761793981194685 1102 104-105 0.8839370191189033 1102 106-107 1.1120474115285468 1102 108-109 1.229198028668602 1102 110-111 1.0780767581890345 1102 112-113 0.9143095313402902 1102 114-115 1.0145457018551909 1102 116-117 1.1860209643337285 1102 118-119 1.3235392828014731 1102 120-121 1.2592304289223115 1102 122-123 1.4095260593100534 1102 124-125 1.2914688157963674 1103 1 1.3579532145758684 1103 2 1.1437316609492711 1103 3 1.3606538586084298 1103 4 1.7983563269963376 1103 5 1.3824836501710216 1103 6 1.2871994051437206 1103 7 1.2520876967613752 1103 8 1.606008507805111 1103 9 2.2396641092700698 1103 10-11 1.7586251184912953 1103 12-13 1.8441764284385478 1103 14-15 2.1219003244385988 1103 16-17 1.9326941374759627 1103 18-19 1.5986498193296725 1103 20-21 2.048326517119783 1103 22-23 1.8543943614158174 1103 24-25 1.8251741941470385 1103 26-27 1.6362533551471188 1103 28-29 1.34589691632776 1103 30-31 1.663489116562502 1103 32-33 1.6604139310976826 1103 34-35 1.4799956614556535 1103 36-37 1.8491497435362412 1103 38-39 1.9344208306472055 1103 40-41 2.110498272369444 1103 42-43 2.0428412836814935 1103 44-45 1.901737001279102 1103 46-47 2.072137810116846 1103 48-49 2.134487112034531 1103 50-51 2.0389684503751724 1103 52-53 1.917602703196792 1103 54-55 1.8522174148847732 1103 56-57 2.01727416213037 1103 58-59 2.2285475227577436 1103 60-61 2.054335905241178 1103 62-63 1.9049930673162656 1103 64-65 1.8644925886603971 1103 66-67 1.790532669393258 1103 68-69 1.7557853849548835 1103 70-71 1.922027187320122 1103 72-73 1.911220394963923 1103 74-75 1.807950575923016 1103 76-77 1.873449004441742 1103 78-79 1.9831971013690044 1103 80-81 1.5304503425110099 1103 82-83 1.7126372932307845 1103 84-85 1.6405469259922185 1103 86-87 1.6182102310416155 1103 88-89 1.7061758426466298 1103 90-91 1.6903454823484942 1103 92-93 1.6922917920335934 1103 94-95 1.4734861551432061 1103 96-97 1.6670362823022877 1103 98-99 1.590904402509146 1103 100-101 1.4467908161196412 1103 102-103 1.5546309791644504 1103 104-105 1.7734719131530738 1103 106-107 1.4454024194145383 1103 108-109 1.7221909950268142 1103 110-111 1.8258595789496859 1103 112-113 1.7570727917872588 1103 114-115 1.7001628013144447 1103 116-117 1.8287668518783846 1103 118-119 1.9327630633002286 1103 120-121 1.816296310914737 1103 122-123 1.985359997424382 1103 124-125 1.9019777143366206 1104 1 1.2261089898793713 1104 2 1.0353782040977357 1104 3 1.1364250863216725 1104 4 1.3644274939602674 1104 5 1.0467317951536508 1104 6 1.0382220350203042 1104 7 1.0933162915425996 1104 8 1.4829144461508648 1104 9 1.9111660928714898 1104 10-11 1.7361423410730161 1104 12-13 1.210450549971135 1104 14-15 1.5100663427767032 1104 16-17 1.2941929092067426 1104 18-19 0.6079010003686136 1104 20-21 -3.6153456861833106 1104 22-23 -0.7610221103224362 1104 24-25 0.6548831802042905 1104 26-27 0.8831854479007006 1104 28-29 0.5443918963176912 1104 30-31 1.3657680508274623 1104 32-33 1.268282207335755 1104 34-35 1.496233154415151 1104 36-37 1.736970369509958 1104 38-39 1.746255717805763 1104 40-41 1.8439806391784614 1104 42-43 1.7221555542313531 1104 44-45 1.9615947171007058 1104 46-47 1.8539182589570942 1104 48-49 1.9227850932984296 1104 50-51 1.8495949046750368 1104 52-53 1.9012125265882993 1104 54-55 1.8052799737112295 1104 56-57 2.0578424560626196 1104 58-59 2.0406570361311545 1104 60-61 2.1250251756412943 1104 62-63 1.8592185023017116 1104 64-65 1.998928418789788 1104 66-67 1.8433085028782692 1104 68-69 1.842849558313592 1104 70-71 2.008229940917847 1104 72-73 1.91256387718839 1104 74-75 1.9956203961843961 1104 76-77 2.026617376014162 1104 78-79 1.9755531240527873 1104 80-81 1.7529780142423306 1104 82-83 1.7274424278830693 1104 84-85 1.6403076388104694 1104 86-87 1.5679167972735506 1104 88-89 1.7179684392354666 1104 90-91 1.7205245761993133 1104 92-93 1.3704808156242088 1104 94-95 1.5434897672645143 1104 96-97 1.6225295711626977 1104 98-99 1.514193596932106 1104 100-101 1.1226010259926298 1104 102-103 1.4983455005192639 1104 104-105 1.7343621206611957 1104 106-107 1.770972880549106 1104 108-109 1.7812929030034077 1104 110-111 1.8786652198345024 1104 112-113 1.8433708515510254 1104 114-115 1.8286033098667396 1104 116-117 1.9311177995528723 1104 118-119 1.9022170671351653 1104 120-121 1.9217478271354267 1104 122-123 1.7341622402656256 1104 124-125 1.7975429968758903 1105 1 1.116085117608538 1105 2 0.8761722120447679 1105 3 0.957166837294313 1105 4 1.5826767372875175 1105 5 1.156415590185368 1105 6 1.0863144130280098 1105 7 1.0422444922304344 1105 8 1.0698781782059008 1105 9 1.5877182052477998 1105 10-11 1.2932930880772915 1105 12-13 1.3665637199243683 1105 14-15 1.4742763330882056 1105 16-17 1.3260940207097391 1105 18-19 0.9311941887834934 1105 20-21 1.070986569887161 1105 22-23 0.9304433955262787 1105 24-25 0.9558418290145987 1105 26-27 0.9699774786727744 1105 28-29 0.5276227023168403 1105 30-31 0.9984315167419311 1105 32-33 0.9308291874098238 1105 34-35 0.8415630511483769 1105 36-37 1.011737510818758 1105 38-39 0.9954255124250011 1105 40-41 1.1141432576709427 1105 42-43 1.283287897375331 1105 44-45 1.6246284463553167 1105 46-47 1.8567882305266252 1105 48-49 1.4490231374971039 1105 50-51 1.5791244967940266 1105 52-53 1.514773026940567 1105 54-55 1.571295659385033 1105 56-57 1.8199155913695257 1105 58-59 1.5384615874380145 1105 60-61 1.9386254118610644 1105 62-63 1.8427025873591383 1105 64-65 1.624956498993523 1105 66-67 1.7835732439447227 1105 68-69 1.4154335582140476 1105 70-71 1.458197109572989 1105 72-73 1.3494608845944995 1105 74-75 1.6641238410799417 1105 76-77 1.51206267088412 1105 78-79 1.3435392589417745 1105 80-81 1.4571852069885693 1105 82-83 1.5464783755954876 1105 84-85 1.4993483943018333 1105 86-87 1.4770062419854533 1105 88-89 1.5931603248945692 1105 90-91 1.6345992400123457 1105 92-93 1.5753516792394713 1105 94-95 1.4357074870657556 1105 96-97 1.6248138203840021 1105 98-99 1.3159673441277455 1105 100-101 1.430317597018604 1105 102-103 1.4264973811381765 1105 104-105 1.5518929110420316 1105 106-107 1.662994227287868 1105 108-109 1.7749663972453753 1105 110-111 1.5744084546895323 1105 112-113 1.6907013793097043 1105 114-115 1.6234108370907983 1105 116-117 1.717970876888959 1105 118-119 1.7617753400190637 1105 120-121 1.7361298548381896 1105 122-123 1.817315934439108 1105 124-125 1.472243545833713 1106 1 -0.24738963750627363 1106 2 -0.01854641510410815 1106 3 0.027561700074564044 1106 4 -0.2489591494816814 1106 5 -0.7789752223012094 1106 6 -0.16940473003723255 1106 7 -0.3004587928262019 1106 8 0.24259927521685398 1106 9 0.1853979822598717 1106 10-11 -2.155727779689375 1106 12-13 -0.46414319638979507 1106 14-15 -0.03982980678349435 1106 16-17 -0.30285880912656893 1106 18-19 -3.0144325153746507 1106 20-21 -5.330171236324375 1106 22-23 -6.080516909261238 1106 24-25 -5.959511702094456 1106 26-27 -4.973922014515264 1106 28-29 -1.986672529075154 1106 30-31 -3.6829450403450323 1106 32-33 -4.041522159808899 1106 34-35 -3.3875986547839574 1106 36-37 -5.6663788224865606 1106 38-39 -5.922833987673052 1106 40-41 -2.386147528903095 1106 42-43 -1.152187539402739 1106 44-45 -0.3102830716527336 1106 46-47 -0.17687049779788566 1106 48-49 0.030214163120167115 1106 50-51 -0.02041219980046094 1106 52-53 -0.16513135928236267 1106 54-55 -0.18729837512767133 1106 56-57 -0.06981822729871467 1106 58-59 0.11918280233935974 1106 60-61 0.3281345384449992 1106 62-63 0.025504771949812266 1106 64-65 -0.1243907267303932 1106 66-67 -0.3297586712022067 1106 68-69 -0.29944881985693783 1106 70-71 -0.12260158032625768 1106 72-73 -0.09340969393217335 1106 74-75 -0.06772410249141458 1106 76-77 -0.07890100136658162 1106 78-79 -0.0968006155574841 1106 80-81 -0.4878626961965722 1106 82-83 -0.6020922617596582 1106 84-85 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-5.683362906484703 2216 102-103 -6.398165430964344 2216 104-105 -8.53360882214222 2216 106-107 -5.867781068306652 2216 108-109 -7.750616314712078 2216 110-111 -5.726473033718413 2216 112-113 -5.249535237417236 2216 114-115 -5.5705451116961555 2216 116-117 -5.190338325644301 2216 118-119 -5.448316096932899 2216 120-121 -6.071303922250079 2216 122-123 -8.477897236986484 2216 124-125 -7.278039611483472 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 607153.0 3 76012.0 4 41453.0 5 33875.0 6 32903.0 7 35574.0 8 38109.0 9 39466.0 10 40444.0 11 40630.0 12 41041.0 13 40137.0 14 39551.0 15 33826.0 16 27994.0 17 24939.0 18 22489.0 19 21533.0 20 21440.0 21 23053.0 22 29011.0 23 39491.0 24 52173.0 25 64980.0 26 75780.0 27 83799.0 28 92814.0 29 102773.0 30 112809.0 31 124025.0 32 141848.0 33 164539.0 34 194679.0 35 231480.0 36 272111.0 37 238478.0 38 54788.0 39 116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.40246306652498 18.50394827703416 13.722424376868739 23.371164279572124 2 14.96936993844192 20.046898557288344 40.84330995357818 24.14042155069156 3 19.02522577986318 25.801050296902716 28.487940180218622 26.685783743015477 4 11.363173084144234 16.50430752395155 38.05569562309791 34.0768237688063 5 13.440920781663046 38.72894817622884 33.77134239928951 14.058788642818612 6 31.58438895420482 36.76518466203997 17.59878136070466 14.051645023050549 7 28.201834130021396 32.77199788397108 20.670987289528995 18.355180696478527 8 25.677569133051076 35.810977051860135 19.937168289734785 18.574285525353996 9 26.313887747016736 16.602771472657622 20.1243945595253 36.95894622080034 10-11 24.55388018617776 27.390359475444022 27.097320099470057 20.95844023890816 12-13 25.569875750263733 24.669864594700826 27.63611238909412 22.12414726594132 14-15 22.31460955651845 25.906723248943997 25.790007779541817 25.988659414995734 16-17 22.189140872871935 29.490721175567526 25.85476028071164 22.4653776708489 18-19 22.00860519255027 27.835834591062685 28.36913070394908 21.786429512437966 20-21 22.88227423432409 27.013556737745187 28.406483802424535 21.697685225506184 22-23 22.45137687927226 27.142120872229597 27.96686314027083 22.439639108227315 24-25 22.117691254358206 27.10065016941286 28.314744616094096 22.46691396013484 26-27 22.218536763223046 27.53346179979547 27.714544105412003 22.533457331569476 28-29 22.073690371758232 27.48190059738082 27.860077952909535 22.58433107795142 30-31 22.10425998483279 27.103303842856725 28.265343993670992 22.527092178639492 32-33 22.047010013487945 27.24905810777964 27.84140465262586 22.86252722610656 34-35 22.489887335172053 26.952329711304195 28.169618093105036 22.388164860418712 36-37 22.310412052847376 26.889400887342635 28.078496611030275 22.721690448779718 38-39 22.255319015838154 27.093718074994893 28.08548495176555 22.565477957401402 40-41 22.539008581999774 27.001708057068324 28.02466514855925 22.43461821237265 42-43 22.38410023891944 27.099243918278827 27.780045162328182 22.73661068047355 44-45 22.38212477547949 27.19535845080513 27.80352258087025 22.618994192845133 46-47 22.39313907264647 27.32768857444941 27.417645682483283 22.861526670420837 48-49 22.585318184059126 27.33509316571285 27.33717839281717 22.74241025741086 50-51 22.542262609074886 27.52851765395601 27.114366725173582 22.81485301179552 52-53 22.813473340152253 27.298574118816926 27.189617367423168 22.69833517360765 54-55 22.57646694866367 27.254246972236068 27.222223297952887 22.94706278114738 56-57 22.540083308522284 27.574802143376626 27.191607367145963 22.693507180955123 58-59 22.758826554049666 27.184705068539593 27.119478208955933 22.936990168454805 60-61 22.70323380342122 27.067870242632097 27.607557559198 22.621338394748683 62-63 22.571237330273476 27.289234076195 27.270169537369178 22.869359056162345 64-65 22.497163793125278 27.135540528081837 27.301877712679023 23.065417966113863 66-67 22.56051460876186 27.556421617428633 26.896881045279684 22.986182728529815 68-69 22.64307249328043 27.272059736900978 27.100924132265035 22.983943637553555 70-71 22.32294134047593 27.1420490561002 27.54155625509363 22.99345334833024 72-73 22.58363097019938 27.18761234916729 27.23973789488452 22.989018785748815 74-75 22.462577536841636 27.310929250657157 27.171068798355808 23.0554244141454 76-77 22.612896249481675 27.12022665637169 27.325187907211728 22.94168918693491 78-79 22.642520428691558 27.22100526069997 27.249636339124844 22.886837971483626 80-81 22.416664457471907 27.53965306011204 27.05746279393336 22.986219688482688 82-83 22.676806874204026 27.50797146551199 26.940701292368587 22.874520367915395 84-85 22.68682110646075 27.26592237778464 26.921350443180632 23.125906072573972 86-87 22.708362655013953 27.142217783943707 27.059647931322857 23.08977162971949 88-89 22.648863660566263 27.178508304926897 27.07060182030569 23.10202621420115 90-91 22.6374816564776 27.391136695936375 27.05839529011771 22.912986357468316 92-93 22.6809692600977 27.306773501727633 27.035252591445254 22.97700464672942 94-95 22.604945709198496 27.234881346617406 26.946631110740427 23.21354183344367 96-97 22.723682813318423 27.082550514787435 26.952578434881186 23.241188237012956 98-99 22.47450925607925 27.091602255332496 26.947389773439106 23.486498715149146 100-101 22.487437054957642 27.19233276475343 27.0123163850054 23.307913795283532 102-103 22.6335343932853 26.939462045360685 27.246450672052358 23.180552889301655 104-105 22.470356002015983 26.920381201797227 27.331679759035282 23.27758303715151 106-107 22.388571842170755 27.036224045817953 27.309554903938043 23.265649208073246 108-109 22.458705982784803 27.065788054923264 27.017668543392155 23.457837418899782 110-111 22.30173537074317 27.111211855345907 27.2181301496037 23.368922624307224 112-113 22.464162146117435 27.173516261243286 27.1100550627545 23.252266529884775 114-115 22.27505021257127 27.236079061677987 27.04066481057134 23.4482059151794 116-117 22.175797089037907 27.137732666594683 27.309322302110058 23.377147942257352 118-119 22.310132874259818 27.174340256818454 26.97929656485702 23.536230304064716 120-121 22.214960630273083 27.214909246401152 27.105052017606347 23.46507810571941 122-123 22.38507009655497 27.113984906863102 27.090363715705706 23.41058128087622 124-125 22.449320490833856 27.189068845276232 26.70770608082585 23.653904583064058 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1167.0 1 1280.0 2 1346.0 3 1161.5 4 934.5 5 837.5 6 827.5 7 974.5 8 1273.5 9 1470.0 10 1488.5 11 1583.0 12 1761.5 13 1925.5 14 2202.5 15 2457.0 16 2697.0 17 2996.5 18 3444.0 19 3936.5 20 4540.0 21 5575.0 22 6861.0 23 8557.0 24 10881.5 25 13984.5 26 18244.5 27 23060.0 28 27803.0 29 33158.0 30 39667.0 31 46163.5 32 52527.0 33 59264.0 34 66433.5 35 72519.0 36 78667.5 37 85859.5 38 90769.0 39 94140.5 40 97974.5 41 103310.0 42 108582.0 43 113415.0 44 117985.5 45 121391.5 46 123571.5 47 125477.5 48 128655.5 49 130024.0 50 127996.0 51 123906.5 52 119351.0 53 116049.0 54 110996.5 55 102722.5 56 93455.5 57 84407.0 58 76666.5 59 68344.0 60 58171.0 61 47721.0 62 38826.5 63 31038.5 64 24357.0 65 19627.0 66 15711.0 67 12321.5 68 9420.5 69 7029.0 70 5417.5 71 4282.0 72 3457.5 73 2868.0 74 2340.0 75 1860.0 76 1375.0 77 965.0 78 670.5 79 462.0 80 341.5 81 261.5 82 178.5 83 107.0 84 80.5 85 72.5 86 53.5 87 36.0 88 27.0 89 21.0 90 15.5 91 13.0 92 8.5 93 6.0 94 6.0 95 5.0 96 2.5 97 2.0 98 2.5 99 4.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10270108622482961 2 0.03383655277012947 3 0.04476790388512729 4 0.0533759705669648 5 0.05677154012312216 6 0.003842355024072801 7 0.002501998620326475 8 0.0027998555989367695 9 0.007625138652423543 10-11 0.06360735778222842 12-13 0.007342174522743763 14-15 0.012941885720617302 16-17 0.02309880869122835 18-19 0.021981845021439746 20-21 0.1367759245778473 22-23 0.018794775350309594 24-25 0.017141669119022457 26-27 0.00831020970322722 28-29 0.004795497355625743 30-31 0.0022041416417161804 32-33 0.00726771027809119 34-35 0.01888413244389268 36-37 0.003008355483963976 38-39 0.00975481604948715 40-41 0.03465565946130778 42-43 0.015965134053511795 44-45 0.11260483076362189 46-47 0.004780604506695229 48-49 0.010946243963928329 50-51 0.0133439926417412 52-53 0.01428224212436363 54-55 0.012644028742007008 56-57 0.008235745458574648 58-59 0.016977847780786797 60-61 0.03592155162040154 62-63 0.00887613796258678 64-65 0.008325102552157735 66-67 0.010663279834248548 68-69 0.009799494596278694 70-71 0.0053614256149853035 72-73 0.0011020708208580902 74-75 0.0012212136123022081 76-77 0.010127137272750018 78-79 0.024528522188557764 80-81 0.004021069211238977 82-83 0.005525246953220966 84-85 0.003187069671130153 86-87 0.00472103311097317 88-89 0.007223031731299645 90-91 0.006091175212580525 92-93 0.0034551409518794184 94-95 0.004869961600278317 96-97 0.0016828919291481648 98-99 0.003484926649740447 100-101 0.0036189622901150803 102-103 7.595352954562514E-4 104-105 0.004289140491988243 106-107 8.786780869003692E-4 108-109 0.0014892848930514733 110-111 0.0023679629799518427 112-113 0.004363604736640817 114-115 0.0026509271096316223 116-117 0.005316747068193759 118-119 0.003559390894393021 120-121 0.006895389054828322 122-123 0.004795497355625743 124-125 0.05488014830894679 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3357316.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.74540195559655 #Duplication Level Percentage of deduplicated Percentage of total 1 85.71921304875457 59.78520969402804 2 8.018321050814647 11.18482049396178 3 2.741261179576496 5.735710885045064 4 1.2050765249442514 3.361941864779612 5 0.6335078093882183 2.2092128403895366 6 0.4053040161341846 1.6960854911697778 7 0.25236115956067 1.2320721358077558 8 0.18158724020612413 1.0131900046546873 9 0.12922625341467217 0.8111643288859884 >10 0.6521451470666677 8.131539993258139 >50 0.04237018853118279 2.021604890752852 >100 0.018830185787688716 2.2521418258568056 >500 6.19263418507496E-4 0.2567383124153762 >1k 1.769324022727463E-4 0.3085672389946273 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4066 0.12110864750294581 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.9785697861029466E-5 0.0 0.0 0.0 5 0.0 2.9785697861029466E-5 0.0 0.0 0.0 6 0.0 2.9785697861029466E-5 0.0 0.0 0.0 7 0.0 5.957139572205893E-5 0.0 5.957139572205893E-5 0.0 8 0.0 5.957139572205893E-5 0.0 5.957139572205893E-5 0.0 9 0.0 5.957139572205893E-5 0.0 1.1914279144411786E-4 0.0 10-11 2.9785697861029466E-5 5.957139572205893E-5 0.0 1.4892848930514734E-4 0.0 12-13 2.9785697861029466E-5 5.957139572205893E-5 0.0 1.6382133823566206E-4 0.0 14-15 2.9785697861029466E-5 5.957139572205893E-5 0.0 1.787141871661768E-4 0.0 16-17 2.9785697861029466E-5 5.957139572205893E-5 0.0 2.0849988502720626E-4 0.0 18-19 2.9785697861029466E-5 5.957139572205893E-5 0.0 2.5317843181875047E-4 0.0 20-21 2.9785697861029466E-5 5.957139572205893E-5 0.0 2.978569786102947E-4 0.0 22-23 2.9785697861029466E-5 5.957139572205893E-5 0.0 4.3189261898492724E-4 0.0 24-25 2.9785697861029466E-5 5.957139572205893E-5 0.0 6.701782018731629E-4 0.0 26-27 2.9785697861029466E-5 7.446424465257367E-5 0.0 7.446424465257366E-4 0.0 28-29 2.9785697861029466E-5 8.93570935830884E-5 0.0 0.0012361064612327229 0.0 30-31 5.957139572205893E-5 8.93570935830884E-5 0.0 0.003246641066852212 0.0 32-33 5.957139572205893E-5 8.93570935830884E-5 0.0 0.007148567486647071 0.0 34-35 5.957139572205893E-5 8.93570935830884E-5 0.0 0.012390850310188257 0.0 36-37 5.957139572205893E-5 8.93570935830884E-5 0.0 0.02123720257491401 0.0 38-39 5.957139572205893E-5 8.93570935830884E-5 0.0 0.03697894389446808 0.0 40-41 5.957139572205893E-5 8.93570935830884E-5 0.0 0.057009825706010396 0.0 42-43 5.957139572205893E-5 8.93570935830884E-5 0.0 0.07754706438119022 2.9785697861029466E-5 44-45 5.957139572205893E-5 8.93570935830884E-5 0.0 0.10030333754701673 2.9785697861029466E-5 46-47 5.957139572205893E-5 8.93570935830884E-5 0.0 0.12880825040002192 4.46785467915442E-5 48-49 5.957139572205893E-5 8.93570935830884E-5 0.0 0.16160230374501536 5.957139572205893E-5 50-51 5.957139572205893E-5 8.93570935830884E-5 0.0 0.20001096113681285 5.957139572205893E-5 52-53 5.957139572205893E-5 8.93570935830884E-5 0.0 0.23620058403796368 5.957139572205893E-5 54-55 5.957139572205893E-5 8.93570935830884E-5 0.0 0.2777963111008913 8.93570935830884E-5 56-57 5.957139572205893E-5 8.93570935830884E-5 0.0 0.3261533915782726 8.93570935830884E-5 58-59 5.957139572205893E-5 8.93570935830884E-5 0.0 0.3839823239754614 8.93570935830884E-5 60-61 5.957139572205893E-5 8.93570935830884E-5 0.0 0.45092567991812504 8.93570935830884E-5 62-63 5.957139572205893E-5 8.93570935830884E-5 0.0 0.5230964258353995 1.0424994251360313E-4 64-65 5.957139572205893E-5 1.1914279144411786E-4 0.0 0.5930630301109576 1.1914279144411786E-4 66-67 5.957139572205893E-5 1.1914279144411786E-4 0.0 0.6762991627836046 1.1914279144411786E-4 68-69 5.957139572205893E-5 1.1914279144411786E-4 0.0 0.7746515371207239 1.1914279144411786E-4 70-71 5.957139572205893E-5 1.1914279144411786E-4 0.0 0.8918731510528053 1.340356403746326E-4 72-73 5.957139572205893E-5 1.1914279144411786E-4 0.0 1.0112244423819505 1.4892848930514734E-4 74-75 5.957139572205893E-5 1.1914279144411786E-4 0.0 1.1311565548193854 1.4892848930514734E-4 76-77 7.446424465257367E-5 1.1914279144411786E-4 0.0 1.2648049811218247 1.4892848930514734E-4 78-79 8.93570935830884E-5 1.1914279144411786E-4 0.0 1.425513713931009 1.4892848930514734E-4 80-81 8.93570935830884E-5 1.1914279144411786E-4 0.0 1.6095744338632407 1.4892848930514734E-4 82-83 8.93570935830884E-5 1.1914279144411786E-4 0.0 1.8072770034158239 1.4892848930514734E-4 84-85 8.93570935830884E-5 1.1914279144411786E-4 0.0 2.005694429717072 1.4892848930514734E-4 86-87 8.93570935830884E-5 1.1914279144411786E-4 0.0 2.209249888899347 1.4892848930514734E-4 88-89 8.93570935830884E-5 1.1914279144411786E-4 0.0 2.4446611519439934 1.4892848930514734E-4 90-91 8.93570935830884E-5 1.1914279144411786E-4 0.0 2.714594038809573 1.4892848930514734E-4 92-93 8.93570935830884E-5 1.1914279144411786E-4 0.0 2.999360203209945 1.4892848930514734E-4 94-95 8.93570935830884E-5 1.1914279144411786E-4 0.0 3.2917812919606018 1.4892848930514734E-4 96-97 8.93570935830884E-5 1.1914279144411786E-4 0.0 3.5815365607526966 1.4892848930514734E-4 98-99 8.93570935830884E-5 1.1914279144411786E-4 0.0 3.893854495674521 1.4892848930514734E-4 100-101 8.93570935830884E-5 1.1914279144411786E-4 0.0 4.259861150990851 1.4892848930514734E-4 102-103 8.93570935830884E-5 1.1914279144411786E-4 0.0 4.655147147304573 1.4892848930514734E-4 104-105 8.93570935830884E-5 1.1914279144411786E-4 0.0 5.051460750194501 1.4892848930514734E-4 106-107 8.93570935830884E-5 1.1914279144411786E-4 0.0 5.4495317092582285 1.4892848930514734E-4 108-109 8.93570935830884E-5 1.1914279144411786E-4 0.0 5.860753053927602 1.4892848930514734E-4 110-111 8.93570935830884E-5 1.340356403746326E-4 0.0 6.320614443204035 1.4892848930514734E-4 112-113 8.93570935830884E-5 1.4892848930514734E-4 0.0 6.829994555174431 1.4892848930514734E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4110 0.0 53.445892 1 GTATCAA 7570 0.0 52.766273 1 ATCAACG 9160 0.0 42.156578 3 TCAACGC 9375 0.0 41.24203 4 CAACGCA 9525 0.0 40.716427 5 TATCAAC 10190 0.0 39.296196 2 AACGCAG 9770 0.0 38.700718 6 GTACATG 13300 0.0 36.567913 1 TACATGG 13325 0.0 35.051945 2 ACATGGG 13690 0.0 32.98789 3 ACGCAGA 11445 0.0 32.672115 7 CGCAGAG 11360 0.0 32.44872 8 GCAGAGT 12250 0.0 27.368912 9 CATGGGG 6505 0.0 24.544495 4 CATGGGA 8520 0.0 24.403517 4 AGAGTAC 11555 0.0 23.281841 10-11 GAGTACT 6115 0.0 23.151382 12-13 GAGTACA 10060 0.0 22.959557 1 CAGAGTA 12175 0.0 22.902847 10-11 AGTACAT 10135 0.0 21.545273 2 >>END_MODULE