FastQCFastQC Report
Thu 26 May 2016
SRR1364513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364513_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357316
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70700.2105848838774783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63030.18773925361806873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40770.12143629017941714No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58600.060.364421
GTATCAA103700.052.2313271
ATCAACG134150.038.6945153
TCAACGC135500.038.0011184
TATCAAC143250.037.484532
CAACGCA138750.036.833575
GTACATG143850.035.9113731
AACGCAG141800.035.705656
TACATGG143000.034.799452
ACATGGG143450.031.740573
CGCAGAG172750.029.8132138
ACGCAGA168800.029.6772257
GCAGAGT187550.026.2873279
CATGGGA91200.025.0279834
CATGGGG67550.024.7915044
AGAGTAC176900.024.44153410-11
GTGGTAT32800.023.824371
GAGTACT113100.023.82086412-13
GAGTACA113900.023.043741
AGTACTT123900.022.70448712-13
Produced by FastQC (version 0.11.5)