##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364513_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3357316 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.885009930551668 31.0 28.0 31.0 25.0 34.0 2 29.107467095739572 31.0 28.0 33.0 25.0 34.0 3 29.18205078104057 31.0 28.0 34.0 25.0 34.0 4 32.582167719690375 35.0 32.0 37.0 26.0 37.0 5 31.703204881518452 35.0 32.0 37.0 20.0 37.0 6 31.73772948390917 35.0 32.0 37.0 19.0 37.0 7 31.165751749314037 35.0 31.0 37.0 17.0 37.0 8 31.294133766377666 35.0 32.0 37.0 17.0 37.0 9 31.878731701156518 37.0 30.0 39.0 11.0 39.0 10-11 32.06442765590132 37.0 31.0 39.0 15.5 39.0 12-13 31.80665463125902 37.0 30.5 39.0 11.5 39.0 14-15 32.75329400032645 37.5 31.0 40.0 12.0 41.0 16-17 32.81563159380886 38.0 31.0 40.0 11.5 41.0 18-19 32.669767606028145 38.0 31.0 40.0 10.0 41.0 20-21 32.55641426067728 38.0 30.5 40.0 10.0 41.0 22-23 32.52893725225746 37.5 30.5 40.0 10.0 41.0 24-25 32.32971456961454 37.0 30.0 40.0 10.0 41.0 26-27 32.416620151335174 37.0 30.0 40.0 10.0 41.0 28-29 32.295448953866725 37.0 30.0 40.0 9.0 41.0 30-31 31.775984000314537 37.0 30.0 40.0 8.0 41.0 32-33 31.717169608103617 37.0 30.0 40.0 7.5 41.0 34-35 31.517715341659827 37.0 30.0 40.0 2.0 41.0 36-37 31.498136159956346 37.0 30.0 40.0 2.0 41.0 38-39 31.267618240284797 36.0 30.0 40.0 2.0 41.0 40-41 30.96953906036846 36.0 29.0 40.0 2.0 41.0 42-43 30.856740026854787 36.0 28.5 40.0 2.0 41.0 44-45 30.782620700583443 36.0 29.0 40.0 2.0 41.0 46-47 30.628584261952106 36.0 28.0 40.0 2.0 41.0 48-49 30.357757804150697 36.0 28.0 40.0 2.0 41.0 50-51 30.00953812509755 35.5 27.0 40.0 2.0 41.0 52-53 29.917372091277677 35.0 27.0 40.0 2.0 41.0 54-55 29.446750767577434 35.0 26.0 39.5 2.0 41.0 56-57 29.47231553419458 35.0 26.0 39.0 2.0 41.0 58-59 29.230917494808352 35.0 26.0 39.0 2.0 41.0 60-61 28.94830721921916 34.5 26.0 39.0 2.0 41.0 62-63 28.679467169608102 34.0 25.5 39.0 2.0 40.5 64-65 28.208126670233007 34.0 24.0 38.0 2.0 40.0 66-67 27.8177352087203 34.0 23.0 38.0 2.0 40.0 68-69 27.640694382059955 34.0 22.5 37.5 2.0 40.0 70-71 27.719144995585758 34.0 24.5 37.0 2.0 40.0 72-73 27.497545062782294 34.0 23.5 37.0 2.0 39.0 74-75 27.127881766268054 34.0 22.5 36.0 2.0 39.0 76-77 27.05524159775249 33.5 24.0 36.0 2.0 39.0 78-79 26.65169498492248 33.0 22.0 35.5 2.0 37.5 80-81 26.422023276927163 33.0 22.0 35.0 2.0 37.0 82-83 26.288397785612077 33.0 22.0 35.0 2.0 37.0 84-85 25.85317884881852 33.0 20.0 35.0 2.0 36.0 86-87 25.726430428354078 33.0 20.0 35.0 2.0 36.0 88-89 25.68821478228442 33.0 20.0 35.0 2.0 36.0 90-91 25.527309314940865 33.0 20.0 35.0 2.0 35.0 92-93 25.54347445995551 33.0 20.0 35.0 2.0 35.0 94-95 25.558350926752205 33.0 20.0 35.0 2.0 35.0 96-97 25.442447925664432 33.0 20.0 35.0 2.0 35.0 98-99 25.305180834928855 33.0 19.5 35.0 2.0 35.0 100-101 25.209065813286564 33.0 18.5 35.0 2.0 35.0 102-103 24.081888329844436 30.5 17.5 34.0 2.0 35.0 104-105 25.02780956573644 32.0 19.0 35.0 2.0 35.0 106-107 25.128074926518682 33.0 18.0 35.0 2.0 35.0 108-109 25.202408263029156 33.0 18.5 35.0 2.0 35.0 110-111 25.137409168514374 33.0 18.0 35.0 2.0 35.0 112-113 25.089448535675523 33.0 18.0 35.0 2.0 35.0 114-115 25.041540772450375 33.0 17.0 35.0 2.0 35.0 116-117 24.961404139497148 33.0 14.0 35.0 2.0 35.0 118-119 24.837757154822484 33.0 6.5 35.0 2.0 35.0 120-121 24.676156042505383 33.0 2.0 35.0 2.0 35.0 122-123 24.3895629723267 33.0 2.0 35.0 2.0 35.0 124-125 23.625458699747057 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8962294626379155 1101 2 0.8749994405731059 1101 3 0.849020084509231 1101 4 0.8344479180567532 1101 5 0.9085359913539186 1101 6 1.1076987757018237 1101 7 1.501985885161492 1101 8 1.3047946148464185 1101 9 1.976922857969246 1101 10-11 1.7335326843421512 1101 12-13 1.8505120958697638 1101 14-15 1.7442020004789 1101 16-17 1.6898390739586517 1101 18-19 1.8114393296146076 1101 20-21 2.034810135184344 1101 22-23 2.0459902319194967 1101 24-25 2.1188318547577083 1101 26-27 1.7760545991861818 1101 28-29 1.860272680057676 1101 30-31 2.275154709130369 1101 32-33 2.1733859291368844 1101 34-35 2.1690468794744326 1101 36-37 2.090553934239839 1101 38-39 2.264168849725241 1101 40-41 2.3090510787982126 1101 42-43 2.2944091957319337 1101 44-45 2.1679652248392713 1101 46-47 1.9183683850303126 1101 48-49 2.025584850977097 1101 50-51 2.1708138400274883 1101 52-53 2.099672848524243 1101 54-55 2.4415844821152533 1101 56-57 2.130034900874776 1101 58-59 2.1297817357230784 1101 60-61 2.2547825688711143 1101 62-63 2.239201141371275 1101 64-65 2.422009172555555 1101 66-67 2.598664275455839 1101 68-69 2.472566887491844 1101 70-71 2.0483514893390975 1101 72-73 2.2783121060345337 1101 74-75 2.425806206707559 1101 76-77 2.1332917569039793 1101 78-79 2.180162062955713 1101 80-81 2.2712386426951596 1101 82-83 2.2337882301859686 1101 84-85 2.415973330189143 1101 86-87 2.288185015533614 1101 88-89 2.1558732837460184 1101 90-91 2.1214114118498806 1101 92-93 2.03718677259322 1101 94-95 1.928169685593133 1101 96-97 1.7834905901138 1101 98-99 1.9042702608689126 1101 100-101 1.9691072448200515 1101 102-103 1.884517472843573 1101 104-105 1.9916502884793061 1101 106-107 2.065649393494887 1101 108-109 2.027725655437031 1101 110-111 2.039662084298463 1101 112-113 1.9468237684772092 1101 114-115 1.9629739324122681 1101 116-117 1.9550551438257386 1101 118-119 1.9082463155896434 1101 120-121 1.7746670338728556 1101 122-123 1.8680608604283542 1101 124-125 1.7772330473743914 1102 1 0.30521482120301613 1102 2 -1.7121010274675292 1102 3 -0.15128573654243738 1102 4 0.3675381479255222 1102 5 0.6809877654464778 1102 6 0.2750653020828828 1102 7 1.093191076636277 1102 8 0.8234167084121999 1102 9 1.5474587658602807 1102 10-11 1.2219519617926835 1102 12-13 1.3669460752846128 1102 14-15 1.3844289062565522 1102 16-17 1.319711285941409 1102 18-19 1.411049073573679 1102 20-21 1.5100842231010603 1102 22-23 1.5347503560806004 1102 24-25 1.7145165218124916 1102 26-27 1.449695722177509 1102 28-29 1.3808539140639198 1102 30-31 1.7419790435392244 1102 32-33 1.4118023966931332 1102 34-35 1.8226195881680773 1102 36-37 1.0891807267703548 1102 38-39 1.063008275637511 1102 40-41 1.2972093628127652 1102 42-43 1.1283144405704384 1102 44-45 1.1101336424714887 1102 46-47 0.7900650275744496 1102 48-49 0.9793235170678187 1102 50-51 1.1250846496844282 1102 52-53 0.9242477693981819 1102 54-55 1.3106327852994504 1102 56-57 0.9410548730805317 1102 58-59 0.8775361260942063 1102 60-61 0.87056935028453 1102 62-63 1.154229807707324 1102 64-65 1.3637383318310796 1102 66-67 1.5536587495793661 1102 68-69 1.5006749600959282 1102 70-71 0.8701436813579768 1102 72-73 1.2861605608372635 1102 74-75 1.4744410704493411 1102 76-77 1.3155053539021075 1102 78-79 1.2004305305237608 1102 80-81 1.2742332431799106 1102 82-83 0.9220085969942353 1102 84-85 0.9795438742373257 1102 86-87 1.1017466631391173 1102 88-89 1.1580983279360382 1102 90-91 0.9103560891203593 1102 92-93 0.9512007030221774 1102 94-95 1.0165636758621979 1102 96-97 1.1233497862181672 1102 98-99 0.8784946058900225 1102 100-101 0.704126970277887 1102 102-103 0.4701426623714049 1102 104-105 0.8870857953937552 1102 106-107 1.1002431128416 1102 108-109 1.0136325700062017 1102 110-111 0.8546147882699984 1102 112-113 0.8615228836909772 1102 114-115 1.1366890461745278 1102 116-117 0.7330556469555134 1102 118-119 0.34531980677816776 1102 120-121 0.7053851229864243 1102 122-123 1.0484285715952701 1102 124-125 1.0376565509059148 1103 1 0.7308013700754188 1103 2 0.6355352932732785 1103 3 0.5638330344627533 1103 4 0.52407575842971 1103 5 0.8517782513489287 1103 6 0.8814266003564306 1103 7 1.232664771933532 1103 8 1.0120878687513333 1103 9 1.717516197327118 1103 10-11 1.4728430575316764 1103 12-13 1.4178149753947444 1103 14-15 1.1776547271566713 1103 16-17 1.207557615913025 1103 18-19 1.2993569994531455 1103 20-21 1.5212580370484758 1103 22-23 1.468256708886443 1103 24-25 1.744278287990305 1103 26-27 1.2690219728657155 1103 28-29 1.3526767184428223 1103 30-31 1.9003226265523168 1103 32-33 1.7451418831603434 1103 34-35 1.8587553358359337 1103 36-37 1.7608929077802564 1103 38-39 1.7707676767945735 1103 40-41 1.8932412677542771 1103 42-43 1.8652975690099574 1103 44-45 1.7644045160635002 1103 46-47 1.6142755361514745 1103 48-49 1.7145849875626027 1103 50-51 1.798283006542686 1103 52-53 1.6763664430619016 1103 54-55 1.9149182046782052 1103 56-57 1.7565531538616668 1103 58-59 1.8780665151474345 1103 60-61 1.7786396454597835 1103 62-63 1.928813462278594 1103 64-65 1.990117714774854 1103 66-67 2.2509004493343703 1103 68-69 2.2394533623521227 1103 70-71 2.032972178328084 1103 72-73 2.065620546287043 1103 74-75 2.07242074714706 1103 76-77 1.9376587534143361 1103 78-79 2.073331175483059 1103 80-81 2.154054833340396 1103 82-83 1.8885648731131823 1103 84-85 2.1560601560947603 1103 86-87 1.9268836341164999 1103 88-89 1.8389201959299193 1103 90-91 1.9335431486030892 1103 92-93 1.7776108183751482 1103 94-95 1.6716628388807742 1103 96-97 1.587745203674487 1103 98-99 1.7541627752517641 1103 100-101 1.7599193198592644 1103 102-103 1.632175187726201 1103 104-105 1.5644645137990771 1103 106-107 1.5868017859110743 1103 108-109 1.5870284812653708 1103 110-111 1.5696942568254855 1103 112-113 1.5469919753354198 1103 114-115 1.4181300635636163 1103 116-117 1.4986554489022126 1103 118-119 1.635451931746875 1103 120-121 1.6664054079286323 1103 122-123 1.7083655945472351 1103 124-125 1.622291798673217 1104 1 0.5809930076974865 1104 2 0.5161001427948833 1104 3 0.5475904679032872 1104 4 0.5620929124121332 1104 5 0.8523424427118762 1104 6 0.9890973409124761 1104 7 1.3448436174598193 1104 8 1.0456959938961923 1104 9 1.5106354021925306 1104 10-11 1.12218732644601 1104 12-13 1.1050754195588262 1104 14-15 1.2298354167956163 1104 16-17 1.113660466099411 1104 18-19 1.0352872725460003 1104 20-21 1.3534069490396234 1104 22-23 1.4415686922292466 1104 24-25 1.649427653716316 1104 26-27 1.5047709880764444 1104 28-29 1.4490761924232487 1104 30-31 1.9355282486366363 1104 32-33 1.8110321093421362 1104 34-35 1.8324714102760744 1104 36-37 1.6733688513412481 1104 38-39 1.767535687915906 1104 40-41 1.8849064704759542 1104 42-43 1.7088288382759806 1104 44-45 1.4489739263286765 1104 46-47 1.593424978122421 1104 48-49 1.4042673358106974 1104 50-51 1.7765446918361611 1104 52-53 1.548593976121868 1104 54-55 1.9833418660599413 1104 56-57 1.8114093218829481 1104 58-59 1.9167176929590042 1104 60-61 1.9855103990944336 1104 62-63 2.0061672585680554 1104 64-65 2.171899727975095 1104 66-67 2.199753153355278 1104 68-69 2.3504368818196326 1104 70-71 2.0986158064115017 1104 72-73 2.1781400100610817 1104 74-75 2.257732065707337 1104 76-77 2.0066297470091286 1104 78-79 2.0399505167367025 1104 80-81 1.9654452872352195 1104 82-83 2.0536471144069743 1104 84-85 2.3591151573712636 1104 86-87 2.2306612217282336 1104 88-89 2.0586790914128414 1104 90-91 2.073089150898756 1104 92-93 1.850274599995565 1104 94-95 1.8210991901111626 1104 96-97 1.882164976564649 1104 98-99 1.9034509350831286 1104 100-101 1.9513595471790879 1104 102-103 1.8299609559541459 1104 104-105 1.6329443899637788 1104 106-107 1.7918106692913511 1104 108-109 1.7793461418960739 1104 110-111 1.8063534018802052 1104 112-113 1.6790869556635926 1104 114-115 1.6252374090300385 1104 116-117 1.757847473174877 1104 118-119 1.6807136227481472 1104 120-121 1.5959390588270388 1104 122-123 1.8139811043168415 1104 124-125 1.601026667630233 1105 1 0.49777591777750274 1105 2 0.6016527563301217 1105 3 0.3711845983939739 1105 4 0.7454866522734633 1105 5 0.8787973482768798 1105 6 0.9507751663747221 1105 7 1.1535973865210956 1105 8 1.1932090642183404 1105 9 1.6705458271850269 1105 10-11 1.3474559055832032 1105 12-13 1.4450175896227613 1105 14-15 1.372559730469618 1105 16-17 1.3639958950322395 1105 18-19 1.615721135442989 1105 20-21 1.7686298338957869 1105 22-23 1.654226720340489 1105 24-25 1.8153016232583212 1105 26-27 1.665240210996913 1105 28-29 1.6386640864601745 1105 30-31 1.8765354672417587 1105 32-33 1.7729408483581892 1105 34-35 1.902678355732423 1105 36-37 1.706708542127334 1105 38-39 1.81439696200378 1105 40-41 1.9927281897657423 1105 42-43 2.0360698868537845 1105 44-45 1.8989294384618987 1105 46-47 1.769303139721579 1105 48-49 1.9205017779295588 1105 50-51 2.1133481824710394 1105 52-53 1.9952850542628973 1105 54-55 2.2242777581929225 1105 56-57 1.9913967650198607 1105 58-59 1.9273392516033212 1105 60-61 1.7398374537816892 1105 62-63 1.6787368451434048 1105 64-65 2.024663482170368 1105 66-67 2.2527214973882934 1105 68-69 2.2532965591785477 1105 70-71 2.1162557533264135 1105 72-73 2.311396117148906 1105 74-75 2.3588075729376676 1105 76-77 2.121727996471286 1105 78-79 2.2777240767128717 1105 80-81 2.190194793585089 1105 82-83 2.0738608463904953 1105 84-85 2.3717629663599133 1105 86-87 2.292542550039684 1105 88-89 2.15199525247251 1105 90-91 2.1844270278442117 1105 92-93 2.048608514724748 1105 94-95 1.9308723041359777 1105 96-97 1.9654365410586863 1105 98-99 2.070786403633832 1105 100-101 2.0785876412848374 1105 102-103 1.8661950576739592 1105 104-105 1.5090589822987361 1105 106-107 1.7080182263217765 1105 108-109 1.7656688224555985 1105 110-111 1.8717452204892737 1105 112-113 1.8684844300457044 1105 114-115 1.7281774591802588 1105 116-117 1.7837514471763996 1105 118-119 1.8852663813105437 1105 120-121 1.916080320139944 1105 122-123 2.0338756335320873 1105 124-125 1.8666010502564916 1106 1 0.4605622396906419 1106 2 0.47106054970211275 1106 3 0.4838668032477891 1106 4 0.39192519519490077 1106 5 0.5779203488091404 1106 6 0.7176714274984413 1106 7 0.9494036400811474 1106 8 0.7461281353821185 1106 9 1.3692106893116325 1106 10-11 1.1270723649495409 1106 12-13 1.0103956909571359 1106 14-15 1.1588237197404254 1106 16-17 1.1436867654135057 1106 18-19 1.2090347367260108 1106 20-21 1.0614818242591184 1106 22-23 1.1065616504466576 1106 24-25 0.8370162164547708 1106 26-27 0.9343655926816439 1106 28-29 0.9541117839791013 1106 30-31 1.331899071559583 1106 32-33 1.2210521508952752 1106 34-35 1.1408887328882962 1106 36-37 1.1715092139858463 1106 38-39 1.3253512847293116 1106 40-41 1.498719443699315 1106 42-43 1.587317482356685 1106 44-45 1.5053653492760688 1106 46-47 1.4353422734956567 1106 48-49 1.3652216890331985 1106 50-51 1.6909651449141947 1106 52-53 1.3014914873431742 1106 54-55 1.7878198042098568 1106 56-57 1.659197105384596 1106 58-59 1.612348962970291 1106 60-61 1.8316510165460649 1106 62-63 1.8892126320909561 1106 64-65 1.9282876487670038 1106 66-67 1.988457051729931 1106 68-69 1.9284297403048782 1106 70-71 1.778521227315828 1106 72-73 1.6732552778160006 1106 74-75 1.9924729544823379 1106 76-77 1.6264767323594533 1106 78-79 1.566827794578984 1106 80-81 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-4.603850300557543 2216 106-107 -4.713197218939733 2216 108-109 -4.850392959033144 2216 110-111 -4.497957399651465 2216 112-113 -4.515227819449819 2216 114-115 -4.3178205598598645 2216 116-117 -4.250898437983544 2216 118-119 -4.378284807804928 2216 120-121 -4.316704081589144 2216 122-123 -4.300117916001039 2216 124-125 -4.259396225871797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 172718.0 3 56972.0 4 27209.0 5 22160.0 6 22825.0 7 26317.0 8 29191.0 9 30722.0 10 32606.0 11 35280.0 12 38330.0 13 40737.0 14 41367.0 15 34188.0 16 25283.0 17 21611.0 18 19436.0 19 17777.0 20 17620.0 21 18945.0 22 22866.0 23 31451.0 24 43577.0 25 57743.0 26 71081.0 27 80892.0 28 91161.0 29 102680.0 30 115084.0 31 129746.0 32 151271.0 33 180113.0 34 218571.0 35 275700.0 36 383858.0 37 492730.0 38 176564.0 39 934.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.00327331071018 19.663091707265 13.764274518000674 24.569360464024147 2 15.73334477106516 20.5270351939604 39.03819993929662 24.70142009567782 3 19.221515043564562 25.230422158652928 29.255929438873196 26.292133358909318 4 12.635759004622452 17.154996745991273 36.459548986333765 33.749695263052516 5 13.061654011716502 38.95156130671048 33.97639662158701 14.010388059986012 6 32.03244142933394 37.22757968279385 16.958924282282968 13.781054605589233 7 27.722440373566215 32.65584577729651 20.750404204080002 18.87130964505727 8 26.043154710488974 36.209698461509134 19.227978540000404 18.519168288001488 9 25.884665012170437 15.878427887038338 19.832628206579304 38.40427889421193 10-11 25.03769031879688 27.023455940490177 27.033895894447152 20.904957846265795 12-13 25.711696893896363 24.175861326372168 27.804141610517757 22.30830016921371 14-15 22.482565587733227 25.465977540860997 25.870141962417982 26.181314908987797 16-17 22.27135247005221 28.939724935481486 26.285366033833196 22.503556560633104 18-19 22.098634152008113 27.120028118209667 28.865188971347127 21.916148758435092 20-21 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26.766232594132198 28.25553656196562 22.564578454001207 46-47 22.41510496134179 26.946206148826107 27.775397398063777 22.86329149176833 48-49 22.656459896214653 27.08619152095991 27.539857755336556 22.717490827488877 50-51 22.46807757450354 27.173469314780803 27.350900550306502 23.00755256040916 52-53 22.84989854236089 27.020764376268218 27.350939181264778 22.77839790010611 54-55 22.560820294767332 26.876357469369882 27.43057481837276 23.13224741749002 56-57 22.565382819071495 27.36791187214503 27.213710763159966 22.852994545623513 58-59 22.733821521066684 27.043515428251446 27.242483949227285 22.980179101454585 60-61 22.72790228861388 27.003996644938994 27.504202761968195 22.763898304478936 62-63 22.558624248557432 27.01560855957591 27.53355568512425 22.892211506742413 64-65 22.466390988300834 26.811953904320063 27.432669838522656 23.288985268856447 66-67 22.37614373928414 27.211075316449 27.25480668870966 23.1579742555572 68-69 22.475252862679266 26.96907028565284 27.429788663781174 23.125888187886716 70-71 22.30641732709741 26.984028877502798 27.571643822090063 23.137909973309725 72-73 22.496874007991213 26.96770578267537 27.3312150607985 23.20420514853492 74-75 22.381105501353606 27.113949301884798 27.181164122566592 23.323781074195004 76-77 22.467753756309293 26.97167608682281 27.344376791551067 23.216193365316837 78-79 22.595552179789337 27.117688890874636 27.17623361295103 23.110525316385 80-81 22.211636697236774 27.273114487491473 27.294679371282367 23.220569443989383 82-83 22.474097190026715 27.280981988702564 27.126244988732793 23.11867583253793 84-85 22.33760539669188 26.972274876716995 27.089675204836244 23.60044452175488 86-87 22.462606579049854 26.914967748718954 27.23893261521841 23.38349305701278 88-89 22.390780015622802 27.029709384626955 27.116893251888254 23.462617347861993 90-91 22.341520952073164 27.193627031205086 27.186940120126113 23.277911896595633 92-93 22.415973423300454 27.12486035720259 27.22814501791881 23.231021201578148 94-95 22.28541692668206 27.025953086123355 27.23039593133866 23.45823405585592 96-97 22.407991689595985 26.90664571690266 27.1606491944642 23.524713399037157 98-99 22.047284199640426 26.834888345332995 27.12077147340316 23.997055981623415 100-101 22.122159093870778 26.98051199047321 27.23412582755123 23.663203088104783 102-103 22.412858435149783 26.85051561284819 27.166903913212742 23.56972203878929 104-105 22.258095839199886 26.72878681778364 27.31002579147419 23.70309155154229 106-107 21.942127239532443 26.815696538318512 27.41269172509508 23.82948449705397 108-109 22.18878115732925 26.900684951908012 26.978440516174228 23.93209337458851 110-111 21.93814902191828 26.94044028979736 27.219949653167426 23.901461035116938 112-113 22.047156496249006 27.004800419859887 27.172315459055696 23.775727624835415 114-115 21.864760220307264 26.99662021183024 27.08475622306066 24.053863344801837 116-117 21.81850025436986 26.991546223233083 27.218811097912738 23.97114242448432 118-119 21.83271718226718 27.050905848552688 26.872427315496104 24.243949653684034 120-121 21.729240006725803 27.04315966139251 27.078412037576914 24.149188294304768 122-123 21.801277137534374 27.092341449095525 26.9423387757953 24.164042637574806 124-125 21.719130219012108 26.994255932850148 26.86542177621939 24.421192071918355 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 208.0 1 198.5 2 318.0 3 468.5 4 377.5 5 317.0 6 605.0 7 1370.5 8 2238.0 9 2620.0 10 2192.0 11 1612.0 12 1530.5 13 1689.5 14 1948.0 15 2268.5 16 2649.5 17 3028.0 18 3533.5 19 4089.0 20 4725.5 21 5725.5 22 7080.0 23 8709.5 24 10897.5 25 13771.0 26 17769.0 27 22732.0 28 27525.5 29 32915.0 30 39236.5 31 45654.5 32 52180.5 33 58320.0 34 65288.0 35 71335.0 36 77377.5 37 85180.0 38 90033.0 39 92955.0 40 96708.5 41 101813.5 42 106778.0 43 112238.5 44 117970.0 45 122139.0 46 124104.5 47 125942.0 48 130742.5 49 132924.0 50 130642.5 51 126487.0 52 121149.5 53 118060.0 54 114548.0 55 105369.0 56 94897.0 57 85517.5 58 77518.5 59 69282.0 60 59123.5 61 47885.5 62 37996.5 63 29917.5 64 23485.5 65 18989.0 66 14698.5 67 11455.0 68 8504.5 69 5937.5 70 4519.5 71 3668.0 72 3084.0 73 2756.5 74 2211.5 75 1768.0 76 1337.5 77 846.0 78 543.0 79 347.5 80 243.0 81 198.0 82 143.5 83 87.0 84 56.5 85 46.0 86 31.0 87 18.5 88 7.5 89 3.0 90 1.5 91 2.5 92 2.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10499458496012887 2 2.0849988502720626E-4 3 0.0 4 0.1806502575271437 5 0.0 6 1.1914279144411786E-4 7 0.03985326373805743 8 0.0 9 0.0 10-11 6.403925040121335E-4 12-13 6.850710508036778E-4 14-15 5.659282593595598E-4 16-17 0.004169997700544125 18-19 0.0018169275695227975 20-21 0.0032019625200606677 22-23 0.0017871418716617678 24-25 0.004735925959903685 26-27 0.004408283283432361 28-29 0.0035444980454625062 30-31 9.084637847613987E-4 32-33 5.957139572205893E-5 34-35 0.0011467493676496343 36-37 0.0012509993101632376 38-39 4.7657116577647145E-4 40-41 0.0016531062312871352 42-43 8.488923890393398E-4 44-45 0.001280785008024267 46-47 2.531784318187504E-4 48-49 5.957139572205893E-5 50-51 0.04074683467388831 52-53 1.0424994251360313E-4 54-55 0.13612063922490467 56-57 3.574283743323536E-4 58-59 2.9785697861029466E-5 60-61 0.0 62-63 6.999638997341924E-4 64-65 0.06187978730628871 66-67 0.047701795124438694 68-69 0.0017573561738007384 70-71 0.0029934626350334613 72-73 0.07091974660711116 74-75 0.005048675787444494 76-77 0.0019211775120364005 78-79 0.0015935348355650765 80-81 1.787141871661768E-4 82-83 1.9360703609669152E-4 84-85 0.0 86-87 2.2339273395772098E-4 88-89 0.0012956778569547817 90-91 3.276426764713241E-4 92-93 0.002665819958562137 94-95 0.0042593547941272136 96-97 0.0023381772820908133 98-99 0.0 100-101 0.00138503495053787 102-103 0.0014594991951904438 104-105 1.4892848930514734E-4 106-107 7.446424465257367E-5 108-109 0.0 110-111 1.340356403746326E-4 112-113 1.6382133823566206E-4 114-115 1.4892848930514734E-4 116-117 0.0 118-119 0.0014743920441209586 120-121 0.002844534145728314 122-123 0.0012658921590937523 124-125 0.008548495286115456 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3357316.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.01053423403107 #Duplication Level Percentage of deduplicated Percentage of total 1 78.04493002867912 43.71338225163892 2 11.366733710989548 12.733136552969903 3 4.278093128239882 7.188548448469591 4 2.0955762834758493 4.694973886625906 5 1.1545570289915437 3.233367799873603 6 0.704769617927881 2.3684713687232746 7 0.4621359099095697 1.8119135441935539 8 0.31972409773972843 1.4326334017516607 9 0.24346405221148804 1.2272896468032732 >10 1.1923159043033313 11.924494297949597 >50 0.0880161522081002 3.4220783090779583 >100 0.04675566309402895 4.622576982961419 >500 0.002440351859474411 0.8524917230824772 >1k 3.7961028819874553E-4 0.3684431189550169 >5k 1.084600823424987E-4 0.4061986669237792 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7070 0.2105848838774783 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6303 0.18773925361806873 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4077 0.12143629017941714 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.787141871661768E-4 0.0 10-11 0.0 0.0 0.0 2.0849988502720626E-4 0.0 12-13 0.0 0.0 0.0 2.0849988502720626E-4 0.0 14-15 0.0 0.0 0.0 2.0849988502720626E-4 0.0 16-17 0.0 0.0 0.0 3.723212232628683E-4 1.4892848930514733E-5 18-19 0.0 0.0 0.0 4.4678546791544196E-4 2.9785697861029466E-5 20-21 0.0 0.0 0.0 6.106068061511041E-4 2.9785697861029466E-5 22-23 0.0 0.0 0.0 8.637852379698545E-4 2.9785697861029466E-5 24-25 0.0 0.0 0.0 0.0012212136123022081 2.9785697861029466E-5 26-27 0.0 0.0 0.0 0.0013403564037463259 4.46785467915442E-5 28-29 0.0 0.0 0.0 0.0019062846631058858 5.957139572205893E-5 30-31 0.0 0.0 0.0 0.003946604966586404 5.957139572205893E-5 32-33 0.0 0.0 0.0 0.008295316854296707 5.957139572205893E-5 34-35 0.0 0.0 0.0 0.01419288503078054 5.957139572205893E-5 36-37 0.0 0.0 0.0 0.023530701310213276 5.957139572205893E-5 38-39 0.0 0.0 0.0 0.04077662037174934 5.957139572205893E-5 40-41 0.0 0.0 0.0 0.06198403724880232 5.957139572205893E-5 42-43 0.0 0.0 0.0 0.08411481075954721 5.957139572205893E-5 44-45 1.4892848930514733E-5 0.0 0.0 0.10880715428634063 5.957139572205893E-5 46-47 2.9785697861029466E-5 0.0 0.0 0.14042467256582342 5.957139572205893E-5 48-49 2.9785697861029466E-5 0.0 0.0 0.17603347435868413 5.957139572205893E-5 50-51 5.957139572205893E-5 0.0 0.0 0.21801641549380518 5.957139572205893E-5 52-53 5.957139572205893E-5 0.0 0.0 0.25770585789362693 5.957139572205893E-5 54-55 5.957139572205893E-5 0.0 0.0 0.30283119015308657 5.957139572205893E-5 56-57 5.957139572205893E-5 0.0 0.0 0.35562633961176127 7.446424465257367E-5 58-59 5.957139572205893E-5 0.0 0.0 0.4178039838966603 8.93570935830884E-5 60-61 5.957139572205893E-5 0.0 0.0 0.4889769089355902 8.93570935830884E-5 62-63 5.957139572205893E-5 0.0 0.0 0.5623837613140974 8.93570935830884E-5 64-65 5.957139572205893E-5 0.0 0.0 0.6350906497928702 8.93570935830884E-5 66-67 5.957139572205893E-5 0.0 0.0 0.7220648875470763 8.93570935830884E-5 68-69 5.957139572205893E-5 0.0 0.0 0.8244085453975736 8.93570935830884E-5 70-71 7.446424465257367E-5 0.0 0.0 0.9451150859793954 8.93570935830884E-5 72-73 8.93570935830884E-5 0.0 0.0 1.0660896978419667 8.93570935830884E-5 74-75 8.93570935830884E-5 0.0 0.0 1.188196166223257 8.93570935830884E-5 76-77 8.93570935830884E-5 0.0 0.0 1.3248380551607295 8.93570935830884E-5 78-79 8.93570935830884E-5 0.0 0.0 1.4872445727479926 8.93570935830884E-5 80-81 8.93570935830884E-5 0.0 0.0 1.6732711487390524 8.93570935830884E-5 82-83 8.93570935830884E-5 0.0 0.0 1.8739225023798771 8.93570935830884E-5 84-85 8.93570935830884E-5 0.0 0.0 2.073844106423107 8.93570935830884E-5 86-87 8.93570935830884E-5 0.0 0.0 2.2830439553500477 8.93570935830884E-5 88-89 8.93570935830884E-5 0.0 0.0 2.5244719293626217 8.93570935830884E-5 90-91 8.93570935830884E-5 0.0 0.0 2.803772418205495 8.93570935830884E-5 92-93 8.93570935830884E-5 0.0 0.0 3.1020017180390527 8.93570935830884E-5 94-95 8.93570935830884E-5 0.0 0.0 3.4062179431426767 8.93570935830884E-5 96-97 8.93570935830884E-5 0.0 0.0 3.7099427042315947 8.93570935830884E-5 98-99 8.93570935830884E-5 0.0 0.0 4.0439744128941095 8.93570935830884E-5 100-101 8.93570935830884E-5 0.0 0.0 4.436177589479215 8.93570935830884E-5 102-103 8.93570935830884E-5 0.0 0.0 4.859432356084444 8.93570935830884E-5 104-105 8.93570935830884E-5 0.0 0.0 5.280259588313998 8.93570935830884E-5 106-107 8.93570935830884E-5 0.0 0.0 5.704944068416557 8.93570935830884E-5 108-109 8.93570935830884E-5 0.0 0.0 6.145221361349364 8.93570935830884E-5 110-111 8.93570935830884E-5 0.0 0.0 6.637027911581752 8.93570935830884E-5 112-113 8.93570935830884E-5 0.0 0.0 7.182478503661853 8.93570935830884E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5860 0.0 60.36442 1 GTATCAA 10370 0.0 52.231327 1 ATCAACG 13415 0.0 38.694515 3 TCAACGC 13550 0.0 38.001118 4 TATCAAC 14325 0.0 37.48453 2 CAACGCA 13875 0.0 36.83357 5 GTACATG 14385 0.0 35.911373 1 AACGCAG 14180 0.0 35.70565 6 TACATGG 14300 0.0 34.79945 2 ACATGGG 14345 0.0 31.74057 3 CGCAGAG 17275 0.0 29.813213 8 ACGCAGA 16880 0.0 29.677225 7 GCAGAGT 18755 0.0 26.287327 9 CATGGGA 9120 0.0 25.027983 4 CATGGGG 6755 0.0 24.791504 4 AGAGTAC 17690 0.0 24.441534 10-11 GTGGTAT 3280 0.0 23.82437 1 GAGTACT 11310 0.0 23.820864 12-13 GAGTACA 11390 0.0 23.04374 1 AGTACTT 12390 0.0 22.704487 12-13 >>END_MODULE